FastQCFastQC Report
Thu 26 May 2016
SRR1364126_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364126_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2358603
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN95530.4050278915103559No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21150.054.8745731
GTATCAA33650.047.225121
ATCAACG37200.041.7655833
TCAACGC39150.039.840754
CAACGCA39600.039.2385185
GTACATG92750.039.1437871
AACGCAG40700.038.031746
TACATGG95950.036.9706762
TATCAAC44000.035.305632
ACATGGG95150.035.285093
CATGGGG50100.029.9447564
ACGCAGA51850.029.7397147
CGCAGAG53850.028.5246128
GAGTACA70250.026.433531
AGTACAT68150.025.5019362
CATGGGA57950.024.7583434
GCAGAGT65600.022.417079
GTGGTAT13150.021.725131
ATGGGAG29050.021.7233565
TAGTGCG1954.022046E-521.3721989
Produced by FastQC (version 0.11.5)