FastQCFastQC Report
Thu 26 May 2016
SRR1364126_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364126_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2358603
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58860.24955450323772163No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58290.24713781844591903No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33650.1426691986739608No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA41100.073.686991
GTATCAA70200.050.6997871
ATCAACG90650.038.9198383
TCAACGC91850.038.411364
TATCAAC94000.037.5328032
CAACGCA94250.037.370125
AACGCAG97100.036.5796286
GTGGTAT18400.036.2885061
GTACATG91250.036.1947671
TACATGG94250.034.7188642
ACATGGG93300.033.2868773
TGGTATC19600.031.8725932
ACGCAGA114150.031.0657717
CGCAGAG115750.030.5849488
GCAGAGT128350.027.165249
GAGTACT85900.026.73658412-13
GAGTACA67750.026.5745871
CATGGGG49000.025.2552364
GTACCGT2151.2340752E-724.9050946
CAGAGTA128400.024.8608810-11
Produced by FastQC (version 0.11.5)