##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364126_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2358603 Sequences flagged as poor quality 0 Sequence length 125 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.086391817529275 33.0 31.0 34.0 30.0 34.0 2 32.127969819422766 33.0 31.0 34.0 30.0 34.0 3 32.26122751476191 34.0 31.0 34.0 30.0 34.0 4 35.887627549019484 37.0 35.0 37.0 35.0 37.0 5 35.816307364995296 37.0 35.0 37.0 35.0 37.0 6 35.854463002039765 37.0 35.0 37.0 35.0 37.0 7 35.77631547148884 37.0 35.0 37.0 33.0 37.0 8 35.76493203815988 37.0 35.0 37.0 33.0 37.0 9 37.4362281401321 39.0 37.0 39.0 34.0 39.0 10-11 37.34693651284256 39.0 37.0 39.0 34.0 39.0 12-13 37.28591967363732 39.0 37.0 39.0 33.0 39.0 14-15 38.60858164769569 40.0 38.0 41.0 34.0 41.0 16-17 38.58872031452516 40.0 38.0 41.0 34.0 41.0 18-19 38.708118746563116 40.0 38.0 41.0 34.0 41.0 20-21 38.755491068229794 40.0 38.0 41.0 34.0 41.0 22-23 38.72087905425373 40.0 38.0 41.0 34.0 41.0 24-25 38.69003622059329 40.0 38.0 41.0 34.0 41.0 26-27 38.57366118842383 40.0 38.0 41.0 34.0 41.0 28-29 38.49584754195598 40.0 38.0 41.0 34.0 41.0 30-31 38.37594457397027 40.0 38.0 41.0 34.0 41.0 32-33 38.24461959049488 40.0 38.0 41.0 33.5 41.0 34-35 38.13391804385901 40.0 37.5 41.0 33.0 41.0 36-37 38.024867050537964 40.0 37.0 41.0 33.0 41.0 38-39 37.91534692358146 40.0 37.0 41.0 33.0 41.0 40-41 37.78254415855487 40.0 37.0 41.0 33.0 41.0 42-43 37.638674249121195 40.0 37.0 41.0 33.0 41.0 44-45 37.47530847709428 40.0 37.0 41.0 32.0 41.0 46-47 37.228040708843324 40.0 36.5 41.0 31.5 41.0 48-49 36.98644769806534 40.0 36.0 41.0 31.0 41.0 50-51 36.80717653628016 40.0 36.0 41.0 31.0 41.0 52-53 36.64974881317458 39.5 35.5 41.0 30.5 41.0 54-55 36.46826405291607 39.0 35.0 41.0 30.0 41.0 56-57 36.27022860566191 39.0 35.0 41.0 30.0 41.0 58-59 36.05135455182581 39.0 35.0 41.0 29.5 41.0 60-61 35.79802302464637 38.0 35.0 40.5 29.0 41.0 62-63 35.53336042564179 38.0 35.0 40.0 29.0 41.0 64-65 35.31185875706933 37.0 35.0 40.0 29.0 41.0 66-67 35.105472815899915 37.0 34.0 40.0 29.0 41.0 68-69 34.79580730627409 36.0 34.0 39.0 29.0 41.0 70-71 34.48824388843735 36.0 34.0 39.0 29.0 41.0 72-73 34.16502416896782 35.5 34.0 38.5 29.0 40.5 74-75 33.89385178429774 35.0 34.0 37.0 28.5 39.5 76-77 33.7054436460905 35.0 34.0 37.0 29.0 39.0 78-79 33.437608618321946 35.0 34.0 36.5 29.0 39.0 80-81 33.18955712343281 35.0 34.0 36.0 29.0 38.5 82-83 32.95557412587027 35.0 34.0 36.0 29.0 37.0 84-85 32.76714881648162 35.0 34.0 35.5 29.0 37.0 86-87 32.59446375672379 35.0 33.0 35.0 28.0 36.5 88-89 32.46676295247653 35.0 33.0 35.0 28.0 36.0 90-91 32.3464565677225 35.0 33.0 35.0 27.0 36.0 92-93 32.25395498945774 35.0 33.0 35.0 27.0 36.0 94-95 32.17598828628642 35.0 33.0 35.0 27.0 35.5 96-97 32.12089296079077 35.0 33.0 35.0 27.0 35.0 98-99 32.063467230390195 35.0 33.0 35.0 27.0 35.0 100-101 32.0056132804037 35.0 33.0 35.0 27.0 35.0 102-103 30.801999531078355 34.0 31.0 34.5 24.5 35.0 104-105 31.753204757222814 35.0 33.0 35.0 25.5 35.0 106-107 31.909447880800627 35.0 33.0 35.0 27.0 35.0 108-109 31.906176876735934 35.0 33.0 35.0 27.0 35.0 110-111 31.8686116315463 35.0 33.0 35.0 27.0 35.0 112-113 31.819185127806588 35.0 33.0 35.0 26.5 35.0 114-115 31.752179574095344 35.0 33.0 35.0 25.0 35.0 116-117 31.686113347604493 35.0 33.0 35.0 25.0 35.0 118-119 31.602423553264366 35.0 33.0 35.0 25.0 35.0 120-121 31.488189195044697 35.0 33.0 35.0 25.0 35.0 122-123 31.34472821411658 35.0 33.0 35.0 25.0 35.0 124-125 30.50354786286628 34.5 31.5 35.0 21.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.24826634642654 1101 2 0.30240807018653015 1101 3 0.2536913404406391 1101 4 0.2591037612608531 1101 5 0.21880195566602367 1101 6 0.3045989231767692 1101 7 0.2897615242328939 1101 8 0.3125980954073313 1101 9 0.3194107202054397 1101 10-11 0.30644840560817954 1101 12-13 0.32980633905992107 1101 14-15 0.36853197918181735 1101 16-17 0.4547551677356907 1101 18-19 0.42938021041177166 1101 20-21 0.3472298196468273 1101 22-23 0.4299445014989587 1101 24-25 0.44557742239414466 1101 26-27 0.49936737483655236 1101 28-29 0.5099027475050448 1101 30-31 0.5008674088512137 1101 32-33 0.45458813423782374 1101 34-35 0.5518151270377203 1101 36-37 0.6542578617417689 1101 38-39 0.48340482153079734 1101 40-41 0.4900539900405363 1101 42-43 0.4955065501123812 1101 44-45 0.5240387191950333 1101 46-47 0.5736132460983328 1101 48-49 0.5498583155523562 1101 50-51 0.5165602989281197 1101 52-53 0.5240080393273985 1101 54-55 0.6304024460955802 1101 56-57 0.5714689323705571 1101 58-59 0.5758926166423066 1101 60-61 0.6442489832798444 1101 62-63 0.5459456181365141 1101 64-65 0.5337934823692478 1101 66-67 0.5776969227565019 1101 68-69 0.5931219049891894 1101 70-71 0.5723075475957415 1101 72-73 0.4797681582738207 1101 74-75 0.4234652016837046 1101 76-77 0.4254944164494461 1101 78-79 0.3664856182743108 1101 80-81 0.40639218968642865 1101 82-83 0.33643835887612283 1101 84-85 0.3178212400229654 1101 86-87 0.32668397509448255 1101 88-89 0.3124341845796508 1101 90-91 0.3167834943765371 1101 92-93 0.3543736628157248 1101 94-95 0.39144373878568217 1101 96-97 0.4526204457391785 1101 98-99 0.4817112557354122 1101 100-101 0.4251863587267515 1101 102-103 0.4807120229765687 1101 104-105 0.4754304240659373 1101 106-107 0.447667613591733 1101 108-109 0.4198161028040346 1101 110-111 0.3921121899462534 1101 112-113 0.4901749753768776 1101 114-115 0.4857921870462363 1101 116-117 0.4854392886735077 1101 118-119 0.4643621779865015 1101 120-121 0.5123131719762668 1101 122-123 0.49841441155643906 1101 124-125 0.5877615023620208 1102 1 0.2803317564120533 1102 2 0.3819592624695076 1102 3 0.2568103145665006 1102 4 0.17818517670126965 1102 5 0.24007999698351057 1102 6 0.237413893861536 1102 7 0.19787471840748339 1102 8 0.2869918115127419 1102 9 0.34505904010924837 1102 10-11 0.27820900564776707 1102 12-13 0.3381759040116563 1102 14-15 0.44713381752404047 1102 16-17 0.4354957102446022 1102 18-19 0.4520107358863257 1102 20-21 0.41099551288660763 1102 22-23 0.3907108521623712 1102 24-25 0.39498561892715145 1102 26-27 0.4355989932473463 1102 28-29 0.4156404183679854 1102 30-31 0.5120331016022064 1102 32-33 0.4817016045792215 1102 34-35 0.5284206215351546 1102 36-37 0.5308062899127322 1102 38-39 0.577445972828663 1102 40-41 0.5565302726887111 1102 42-43 0.5076107212087138 1102 44-45 0.5017015904011046 1102 46-47 0.5927563713778596 1102 48-49 0.6618617957428796 1102 50-51 0.6752434231811861 1102 52-53 0.6406420792078436 1102 54-55 0.6517997523694632 1102 56-57 0.6601346067918854 1102 58-59 0.6916900910602166 1102 60-61 0.7287885301218893 1102 62-63 0.6159444939176595 1102 64-65 0.6316105426347178 1102 66-67 0.5682920988800504 1102 68-69 0.6233235556357357 1102 70-71 0.6105185243708462 1102 72-73 0.5811741650240236 1102 74-75 0.5917981562041561 1102 76-77 0.530254627900355 1102 78-79 0.5266144635304784 1102 80-81 0.5208610084990042 1102 82-83 0.5018362468110524 1102 84-85 0.4524276290075804 1102 86-87 0.5104452447774719 1102 88-89 0.4874531863221918 1102 90-91 0.5129879115790388 1102 92-93 0.539987205253766 1102 94-95 0.6003443749469142 1102 96-97 0.5686008501158142 1102 98-99 0.4963845113021321 1102 100-101 0.5699302690043133 1102 102-103 0.6562836027240131 1102 104-105 0.5924557409856739 1102 106-107 0.5463952911266645 1102 108-109 0.5435636374654109 1102 110-111 0.6021967996345765 1102 112-113 0.5980671139099663 1102 114-115 0.634705360691612 1102 116-117 0.554813734201435 1102 118-119 0.647186924487368 1102 120-121 0.733293050902784 1102 122-123 0.7007843137738412 1102 124-125 0.9012070376246122 1103 1 0.24280892547367472 1103 2 0.29058695296381387 1103 3 0.23621937566831264 1103 4 0.14390950126294655 1103 5 0.19354563635321398 1103 6 0.2031034526152098 1103 7 0.23859999558133893 1103 8 0.22427850350256762 1103 9 0.34152802432709706 1103 10-11 0.2627285679469722 1103 12-13 0.32055907474770606 1103 14-15 0.29101966228780896 1103 16-17 0.36607488755784345 1103 18-19 0.35210802934276586 1103 20-21 0.3803352274513401 1103 22-23 0.3979781398958835 1103 24-25 0.3713647926839698 1103 26-27 0.4277491423447586 1103 28-29 0.5176250814934775 1103 30-31 0.46085097340492354 1103 32-33 0.48529257142069326 1103 34-35 0.48191021229659725 1103 36-37 0.4652856973407893 1103 38-39 0.501357445581597 1103 40-41 0.4787131444678394 1103 42-43 0.4639578581645907 1103 44-45 0.46163809743900686 1103 46-47 0.43197477940142903 1103 48-49 0.4583721868364492 1103 50-51 0.4485363512424527 1103 52-53 0.49506653633685005 1103 54-55 0.5373084345675139 1103 56-57 0.5145476325748319 1103 58-59 0.4839312554462367 1103 60-61 0.4301365942869424 1103 62-63 0.5109755236714548 1103 64-65 0.4808279309096335 1103 66-67 0.4829477377453131 1103 68-69 0.4555909431667118 1103 70-71 0.3944209754328938 1103 72-73 0.4143539191303276 1103 74-75 0.4578036555888616 1103 76-77 0.40831918125588373 1103 78-79 0.37144493666843914 1103 80-81 0.29850840844841287 1103 82-83 0.29029138567287305 1103 84-85 0.24858110807424794 1103 86-87 0.13446232139398262 1103 88-89 0.10983300386339323 1103 90-91 0.09757127167623736 1103 92-93 0.13826789451675836 1103 94-95 0.20457310202841938 1103 96-97 0.14122281259849956 1103 98-99 0.1621871859467916 1103 100-101 0.1971449289979752 1103 102-103 0.3214240576770706 1103 104-105 0.29064018920185575 1103 106-107 0.2675657448349753 1103 108-109 0.2403299818407021 1103 110-111 0.2613436002548397 1103 112-113 0.3283073427401142 1103 114-115 0.35946155548320746 1103 116-117 0.4073676239256052 1103 118-119 0.3961618593559173 1103 120-121 0.4254859496054557 1103 122-123 0.5234842815846221 1103 124-125 0.7065950120662983 1104 1 0.24103709897386238 1104 2 0.2874886143090336 1104 3 0.17172910549940212 1104 4 0.15524637633363625 1104 5 0.12235443153255687 1104 6 0.18714294147627442 1104 7 0.2556479313423097 1104 8 0.2590571559698631 1104 9 0.2906495342109139 1104 10-11 0.2790634039583466 1104 12-13 0.26001640130701986 1104 14-15 0.2749101358837365 1104 16-17 0.3100445584385625 1104 18-19 0.28982812157381943 1104 20-21 0.3332245947608925 1104 22-23 0.27958774053129076 1104 24-25 0.3642515517658964 1104 26-27 0.46112572510238437 1104 28-29 0.4190460498016719 1104 30-31 0.4130054688952285 1104 32-33 0.43934193691774936 1104 34-35 0.42861152270994296 1104 36-37 0.419269857147043 1104 38-39 0.4331201322328795 1104 40-41 0.42911088076202475 1104 42-43 0.3968785168378446 1104 44-45 0.5404533210889966 1104 46-47 0.4718332867363344 1104 48-49 0.4421058352201044 1104 50-51 0.44321081459409584 1104 52-53 0.4891742259297658 1104 54-55 0.5169792526631625 1104 56-57 0.4442437040609093 1104 58-59 0.5331827714785504 1104 60-61 0.5618902921311388 1104 62-63 0.5385090871035203 1104 64-65 0.4896789219071991 1104 66-67 0.4915510576359594 1104 68-69 0.4986420242733871 1104 70-71 0.3650445591892364 1104 72-73 0.39465089605451453 1104 74-75 0.36020488278206386 1104 76-77 0.3296660140091845 1104 78-79 0.31480067939257594 1104 80-81 0.29654144378805825 1104 82-83 0.24212482602458607 1104 84-85 0.28114184607004233 1104 86-87 0.32741970155458233 1104 88-89 0.3471386163050809 1104 90-91 0.33629829449859727 1104 92-93 0.3457767903044342 1104 94-95 0.40248769878279944 1104 96-97 0.4037341453107217 1104 98-99 0.4255990571401256 1104 100-101 0.4326790833335785 1104 102-103 0.33286846687851224 1104 104-105 0.3567657223136358 1104 106-107 0.38912805108289916 1104 108-109 0.33435485652236707 1104 110-111 0.31877312561267246 1104 112-113 0.32237041985192505 1104 114-115 0.33810418666912767 1104 116-117 0.38628938704327354 1104 118-119 0.4382443228195463 1104 120-121 0.46290252223962014 1104 122-123 0.5507615166570297 1104 124-125 0.5537145230457234 1105 1 0.18404435509695816 1105 2 0.266040091014645 1105 3 0.1582283807951015 1105 4 0.11006455330222309 1105 5 0.07402749398425357 1105 6 0.12601580779812593 1105 7 0.1567267589671104 1105 8 0.12555833808345795 1105 9 0.2520346558102773 1105 10-11 0.16036024442919228 1105 12-13 0.18883205174189754 1105 14-15 0.1698510529042494 1105 16-17 0.23091570181424714 1105 18-19 0.3264769345229368 1105 20-21 0.2910142296166214 1105 22-23 0.30147006489689687 1105 24-25 0.2527693300052434 1105 26-27 0.27352809999867134 1105 28-29 0.26650050466172814 1105 30-31 0.28992309776717917 1105 32-33 0.37701895601657043 1105 34-35 0.34049939727764666 1105 36-37 0.37656746488508475 1105 38-39 0.38660308267036925 1105 40-41 0.3309363183937535 1105 42-43 0.3257467182659184 1105 44-45 0.3291364703803481 1105 46-47 0.4152831970137427 1105 48-49 0.3863684131244014 1105 50-51 0.3085091124186192 1105 52-53 0.3283014481387383 1105 54-55 0.3569929181914162 1105 56-57 0.3608291607154115 1105 58-59 0.3834141433999747 1105 60-61 0.24481984542499902 1105 62-63 0.3339222820563492 1105 64-65 0.308751981407859 1105 66-67 0.24280984119964444 1105 68-69 0.22429360551347344 1105 70-71 0.22653228575154571 1105 72-73 0.23454345594618786 1105 74-75 0.24136495446933992 1105 76-77 0.19875048036690401 1105 78-79 0.1955470396971677 1105 80-81 0.1547512595871794 1105 82-83 0.20579392955912112 1105 84-85 0.21067232621359722 1105 86-87 0.20853879935731356 1105 88-89 0.21285516555489892 1105 90-91 0.1650804621192421 1105 92-93 0.19227329617414313 1105 94-95 0.16284450073138146 1105 96-97 0.07164818868400857 1105 98-99 0.1661666078665931 1105 100-101 0.22710674853722068 1105 102-103 0.23780122317243269 1105 104-105 0.19069800076806942 1105 106-107 0.15707489626403515 1105 108-109 0.18817206759688787 1105 110-111 0.19749178938733536 1105 112-113 0.13670083734288951 1105 114-115 0.09375977449682438 1105 116-117 0.14144647819257372 1105 118-119 0.1367858341316328 1105 120-121 0.15768238124859835 1105 122-123 0.08409030994775435 1105 124-125 0.16601679000249803 1106 1 0.09569076436873303 1106 2 0.1328808062063871 1106 3 0.03804968421872701 1106 4 0.077510745954541 1106 5 0.0898365879472891 1106 6 0.10079940553462308 1106 7 0.10393474261060476 1106 8 0.1795108468727591 1106 9 0.19723586864758857 1106 10-11 0.15079684269276328 1106 12-13 0.12564291659798954 1106 14-15 0.19449582449814073 1106 16-17 0.18051992965853714 1106 18-19 0.2707154143060819 1106 20-21 0.18767175945971104 1106 22-23 0.19887055895144812 1106 24-25 0.27274621282801803 1106 26-27 0.2657642977479142 1106 28-29 0.2856962325089114 1106 30-31 0.3692759760838058 1106 32-33 0.2771304513279773 1106 34-35 0.32955537226794007 1106 36-37 0.3581116604681398 1106 38-39 0.3847286944466006 1106 40-41 0.39231021574856584 1106 42-43 0.3949649261742678 1106 44-45 0.31942316536972726 1106 46-47 0.40215219871078745 1106 48-49 0.40738882690232003 1106 50-51 0.40411529215485587 1106 52-53 0.4597913802569238 1106 54-55 0.36474187853384166 1106 56-57 0.43025692912951286 1106 58-59 0.35369647993626074 1106 60-61 0.40518329842031164 1106 62-63 0.32597516413774485 1106 64-65 0.41524937067998735 1106 66-67 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122-123 -2.32627430844947 2216 124-125 -2.6799476919049177 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 11.0 3 13.0 4 52.0 5 110.0 6 412.0 7 1014.0 8 1822.0 9 2951.0 10 4929.0 11 6862.0 12 8350.0 13 11510.0 14 15937.0 15 14836.0 16 6981.0 17 5804.0 18 5714.0 19 5180.0 20 5167.0 21 5414.0 22 6027.0 23 7188.0 24 9339.0 25 12401.0 26 16816.0 27 22170.0 28 28890.0 29 37724.0 30 47328.0 31 60979.0 32 76588.0 33 99693.0 34 135863.0 35 202143.0 36 395332.0 37 763993.0 38 331324.0 39 1736.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.059003103727704 18.567324729421877 12.68982089435604 24.68385127249438 2 16.99433170366457 20.16889988242811 37.48240979548088 25.35435861842644 3 19.456602064866363 23.866924615969705 29.64191091082306 27.034562408340868 4 13.446052599780463 15.839588095156328 35.44577022924163 35.26858907582158 5 13.601271600180276 37.7141468911894 34.26184906913118 14.422732439499145 6 33.945899331087084 35.634441234917446 16.101819594056312 14.31783983993915 7 29.047745635870047 32.19833944076218 20.514982809739493 18.238932113628277 8 27.102780756235788 34.86474832771772 19.098805521743166 18.933665394303322 9 26.63924365397653 14.59813287780945 19.3174942964119 39.44512917180212 10-11 25.684894118086003 25.792988154598 27.202344447084908 21.319773280231086 12-13 26.089272899381687 23.136815928308618 27.83360204880975 22.940309123499937 14-15 22.82395553639167 24.706107810428463 25.554745754160408 26.915190899019464 16-17 22.879680487748004 27.914923927168505 26.122288905047284 23.08310668003621 18-19 22.829128089805703 26.192665743238685 28.37641603949457 22.601790127461044 20-21 23.268349951221126 25.562123002472227 28.629256386089562 22.540270660217086 22-23 23.05977309449704 25.492908302075424 28.583826951801555 22.863491651625985 24-25 22.95937468069022 25.715752078666902 28.597245912092877 22.727627328549993 26-27 22.79580327846611 25.9947943761625 28.471239119088715 22.738163226282676 28-29 22.69241580715364 25.908238902435045 28.65204529969647 22.747299990714843 30-31 22.775261457735787 25.640898447089228 28.705127569158524 22.87871252601646 32-33 22.608722196995426 25.78153254278062 28.736268884589734 22.873476375634223 34-35 23.034800684981747 25.754122249484123 28.444761581325896 22.766315484208235 36-37 23.204795380994597 25.72626677741019 28.117682373845877 22.95125546774934 38-39 22.843140621800277 25.767520010786043 28.386676350365025 23.002663017048654 40-41 23.06380090248338 25.684737957172104 28.31300986219385 22.93845127815067 42-43 23.064373275197227 25.768940343075965 28.081326107021827 23.085360274704982 44-45 22.948817583968136 25.959201272956918 28.01291696822229 23.079064174852657 46-47 23.25921318678896 26.10146769083224 27.51970128080054 23.119617841578254 48-49 23.40654616313131 26.187662781739867 27.187873499694522 23.217917555434298 50-51 23.260124743333236 26.31432674341549 27.08132737896119 23.344221134290084 52-53 23.432366532222677 26.284033387560346 27.01855717134253 23.26504290887445 54-55 23.230764143011776 26.237819590664476 27.06284186020284 23.46857440612091 56-57 23.173760060510396 26.3812731519463 27.102505169373565 23.34246161816974 58-59 23.332074113362868 26.137018396059027 27.031615748814026 23.499291741764086 60-61 23.266675231058382 26.198834649154605 27.270591956340258 23.263898163446754 62-63 23.17524398976852 26.18467372423422 27.261900370685527 23.378181915311732 64-65 22.960879808937747 26.160273687432774 27.279919511677043 23.59892699195244 66-67 23.027868615447364 26.323209119974834 27.091630087810454 23.55729217676735 68-69 22.855075652833477 26.00282879314577 27.414278706505502 23.727816847515246 70-71 22.87705900484312 26.07624089344413 27.341269387005784 23.705430714706967 72-73 23.12621072728221 26.21647220833688 26.945272264980584 23.712044799400324 74-75 22.86414882029744 26.290880661137123 26.964563345336202 23.880407173229237 76-77 23.0748031779829 26.208162204491387 26.94031170146057 23.776722916065147 78-79 23.01372888951638 26.182384233378826 26.982497690370106 23.82138918673469 80-81 22.86283448295453 26.48824325246767 26.919430696899816 23.729491567677986 82-83 22.920453336148558 26.413877197646237 26.880148969538325 23.785520496666884 84-85 23.02375601150342 26.143950465593402 26.78721260000093 24.045080922902244 86-87 22.930395662177993 26.13852352430655 26.87722350900088 24.05385730451458 88-89 22.90877269298818 26.20453717730368 26.818544706336763 24.068145423371377 90-91 22.904066517340986 26.32541381487262 26.768451494380358 24.002068173406037 92-93 22.741983865445743 26.121124691422143 26.849034544820505 24.287856898311606 94-95 22.74716424675182 25.982658696080303 26.897824694727372 24.372352362440502 96-97 22.780222531606015 26.060955673669778 26.742886169166507 24.4159356255577 98-99 22.72352908878413 25.87182402382145 26.68162361861426 24.72302326878016 100-101 22.588147152857086 25.92533390205314 26.799655800146997 24.686863144942777 102-103 22.581435027089185 25.760905420634927 26.9529948196019 24.70466473267399 104-105 22.598396917141827 25.859537952899743 26.802726165241435 24.739338964716996 106-107 22.60840102511411 25.813292601295956 26.812412048037963 24.76589432555197 108-109 22.571837091015805 25.7401479182601 26.634531973503723 25.053483017220373 110-111 22.429573246685784 25.965556539115966 26.63949919695532 24.965371017242934 112-113 22.29887070651983 26.039589096459192 26.808644360127104 24.85289583689387 114-115 22.326939000974363 25.958826479385145 26.58454015621637 25.12969436342412 116-117 22.17515490018957 25.97931695483827 26.71670971216767 25.12881843280449 118-119 22.09619697283558 26.004642921431838 26.512993840464404 25.38616626526818 120-121 22.147650722096294 26.16214989832765 26.576621085508666 25.11357829406738 122-123 22.046633574443508 26.261163960855853 26.5174124290048 25.17479003569584 124-125 22.044953258762483 26.27444636371173 26.357452847970496 25.32314752955529 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 325.0 1 250.5 2 235.0 3 292.0 4 198.0 5 145.5 6 389.5 7 882.5 8 1369.5 9 1599.5 10 1371.0 11 1094.5 12 1154.5 13 1342.0 14 1624.0 15 1869.5 16 2107.0 17 2397.0 18 2698.0 19 2980.5 20 3279.0 21 3888.0 22 4647.0 23 5451.0 24 6846.0 25 8888.0 26 11176.0 27 13771.0 28 16470.0 29 19676.5 30 24189.5 31 28355.0 32 32851.0 33 36438.0 34 39500.5 35 42820.5 36 46670.5 37 51163.0 38 53754.5 39 55964.0 40 58996.5 41 63142.0 42 66751.0 43 72161.5 44 77918.0 45 81549.0 46 83026.0 47 85074.5 48 89184.5 49 89603.0 50 87541.0 51 86078.5 52 86542.0 53 88588.5 54 88881.0 55 84801.5 56 78476.0 57 73367.0 58 69046.0 59 62909.0 60 54260.0 61 44298.0 62 35559.5 63 28141.5 64 22234.5 65 17743.0 66 13207.0 67 10631.0 68 8241.0 69 5286.5 70 3705.5 71 2833.0 72 2254.0 73 1921.5 74 1605.5 75 1287.5 76 850.0 77 432.0 78 233.0 79 125.5 80 62.0 81 37.5 82 23.5 83 15.0 84 5.0 85 3.0 86 4.0 87 2.0 88 1.0 89 0.5 90 0.5 91 0.5 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.17022788489627122 2 0.0019503070249635062 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 3.6038281983021307E-4 12-13 0.006804875597970494 14-15 0.0 16-17 3.391838304284358E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 2.119898940177724E-5 94-95 6.147706926515399E-4 96-97 0.0064232937887385025 98-99 0.002395485802400828 100-101 0.0037946191029181254 102-103 0.004112603943944784 104-105 0.011532250234566818 106-107 0.018082737959715985 108-109 0.025926364038373565 110-111 0.016556410722788024 112-113 0.024590827706061597 114-115 0.027791875105729964 116-117 0.026837920582649985 118-119 0.01651401274398447 120-121 0.02281011259631231 122-123 0.014627302687226295 124-125 0.014987685507056507 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2358603.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 70.35217951079693 #Duplication Level Percentage of deduplicated Percentage of total 1 82.83641212815309 58.277221360701816 2 10.757038451642535 15.135622003090013 3 3.15917104809579 6.667637060428425 4 1.264913310648347 3.5595763318531577 5 0.636129568637868 2.237655080246855 6 0.358522335982137 1.5133696643787329 7 0.23076213904114506 1.136423360108268 8 0.15264173504765818 0.8590942995929881 9 0.10918100320820047 0.6913009383184993 >10 0.45863712810502644 5.619387882174927 >50 0.02473184412393909 1.196244420197997 >100 0.01078119539318459 1.4071459745806685 >500 4.7916085380255216E-4 0.20394556292764643 >1k 3.593706403519141E-4 0.5080941317730496 >5k 2.3958042690127608E-4 0.9872819296270156 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5886 0.24955450323772163 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5829 0.24713781844591903 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3365 0.1426691986739608 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 4.239797880355448E-5 12-13 0.0 0.0 0.0 0.0 4.239797880355448E-5 14-15 0.0 0.0 0.0 2.119898940177724E-5 4.239797880355448E-5 16-17 0.0 0.0 0.0 8.479595760710896E-5 4.239797880355448E-5 18-19 0.0 0.0 0.0 1.9079090461599516E-4 4.239797880355448E-5 20-21 0.0 0.0 0.0 2.1198989401777238E-4 4.239797880355448E-5 22-23 0.0 0.0 0.0 3.179848410266586E-4 4.239797880355448E-5 24-25 0.0 0.0 0.0 5.935717032497627E-4 4.239797880355448E-5 26-27 0.0 0.0 0.0 8.691585654728667E-4 4.239797880355448E-5 28-29 0.0 0.0 0.0 0.0024590827706061596 4.239797880355448E-5 30-31 0.0 0.0 0.0 0.008098013951478905 4.239797880355448E-5 32-33 0.0 0.0 0.0 0.019481871260233284 4.239797880355448E-5 34-35 0.0 0.0 0.0 0.03682264459088706 4.239797880355448E-5 36-37 0.0 0.0 0.0 0.06626804086995564 4.239797880355448E-5 38-39 0.0 0.0 0.0 0.10851762674769769 4.239797880355448E-5 40-41 0.0 0.0 0.0 0.15604576098648226 4.239797880355448E-5 42-43 0.0 0.0 0.0 0.20245034878697263 4.239797880355448E-5 44-45 0.0 0.0 0.0 0.2641394079461444 4.239797880355448E-5 46-47 0.0 0.0 0.0 0.3461370989522188 4.239797880355448E-5 48-49 0.0 0.0 0.0 0.4313994343261668 4.239797880355448E-5 50-51 0.0 0.0 0.0 0.5254805492912542 4.239797880355448E-5 52-53 0.0 0.0 0.0 0.6177597501571905 4.239797880355448E-5 54-55 0.0 0.0 0.0 0.7198540831161497 4.239797880355448E-5 56-57 0.0 0.0 0.0 0.8520721800150344 4.239797880355448E-5 58-59 0.0 0.0 0.0 1.00207622902201 4.239797880355448E-5 60-61 0.0 0.0 0.0 1.1624677828358567 4.239797880355448E-5 62-63 0.0 0.0 0.0 1.3232833164377387 4.239797880355448E-5 64-65 0.0 0.0 0.0 1.494952732613331 4.239797880355448E-5 66-67 0.0 0.0 0.0 1.6944352228840547 4.239797880355448E-5 68-69 0.0 0.0 0.0 1.9247834417237661 4.239797880355448E-5 70-71 0.0 0.0 0.0 2.1827751427433952 4.239797880355448E-5 72-73 0.0 0.0 0.0 2.448313683990057 4.239797880355448E-5 74-75 0.0 0.0 0.0 2.720105927110243 4.239797880355448E-5 76-77 0.0 0.0 0.0 3.0194144584739355 4.239797880355448E-5 78-79 0.0 0.0 0.0 3.363558852422387 4.239797880355448E-5 80-81 0.0 0.0 0.0 3.737805811321363 4.239797880355448E-5 82-83 0.0 0.0 0.0 4.135626046435114 4.239797880355448E-5 84-85 0.0 0.0 0.0 4.525348267597387 4.239797880355448E-5 86-87 0.0 0.0 0.0 4.934149579221259 4.239797880355448E-5 88-89 0.0 0.0 0.0 5.388846702899979 4.239797880355448E-5 90-91 0.0 0.0 0.0 5.902922195893078 4.239797880355448E-5 92-93 0.0 0.0 0.0 6.4365855550934175 4.239797880355448E-5 94-95 0.0 0.0 0.0 6.9791736888319065 4.239797880355448E-5 96-97 0.0 0.0 0.0 7.5180096014462805 4.239797880355448E-5 98-99 0.0 0.0 0.0 8.094558516206416 4.239797880355448E-5 100-101 0.0 0.0 0.0 8.735849144599579 4.239797880355448E-5 102-103 0.0 0.0 0.0 9.42136086488485 4.239797880355448E-5 104-105 0.0 0.0 0.0 10.095297936956749 4.239797880355448E-5 106-107 0.0 0.0 0.0 10.7567276052816 4.239797880355448E-5 108-109 0.0 0.0 0.0 11.442642106365504 4.239797880355448E-5 110-111 0.0 0.0 0.0 12.195778602842445 4.239797880355448E-5 112-113 0.0 0.0 0.0 12.989977541790628 4.239797880355448E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4110 0.0 73.68699 1 GTATCAA 7020 0.0 50.699787 1 ATCAACG 9065 0.0 38.919838 3 TCAACGC 9185 0.0 38.41136 4 TATCAAC 9400 0.0 37.532803 2 CAACGCA 9425 0.0 37.37012 5 AACGCAG 9710 0.0 36.579628 6 GTGGTAT 1840 0.0 36.288506 1 GTACATG 9125 0.0 36.194767 1 TACATGG 9425 0.0 34.718864 2 ACATGGG 9330 0.0 33.286877 3 TGGTATC 1960 0.0 31.872593 2 ACGCAGA 11415 0.0 31.065771 7 CGCAGAG 11575 0.0 30.584948 8 GCAGAGT 12835 0.0 27.16524 9 GAGTACT 8590 0.0 26.736584 12-13 GAGTACA 6775 0.0 26.574587 1 CATGGGG 4900 0.0 25.255236 4 GTACCGT 215 1.2340752E-7 24.905094 6 CAGAGTA 12840 0.0 24.86088 10-11 >>END_MODULE