FastQCFastQC Report
Thu 26 May 2016
SRR1364125_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364125_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2764348
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN113480.4105127140287692No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA26350.048.7860371
GTATCAA41700.044.3860741
ATCAACG42550.041.5442473
TCAACGC42800.041.1692544
CAACGCA43950.040.2289245
AACGCAG47750.037.1528056
TATCAAC52200.034.0890732
GTACATG92850.033.9075281
TACATGG98750.030.7360172
ACATGGG95500.030.164493
ACGCAGA58550.029.6907047
CGCAGAG60300.028.4341168
GAGTACA70950.023.9064371
AGTACAT70850.022.5957582
CATGGGA58850.022.5571084
CATGGGG50250.022.15294
GCAGAGT75500.021.9999569
CAGAGTA70650.019.4652710-11
GTACGGT1856.943927E-419.3033873
AGAGTAC64300.019.16549110-11
Produced by FastQC (version 0.11.5)