##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364125_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2764348 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.048204856986167 31.0 31.0 34.0 28.0 34.0 2 31.126199378659994 33.0 31.0 34.0 28.0 34.0 3 31.185245851824735 33.0 31.0 34.0 28.0 34.0 4 34.67096870582141 37.0 35.0 37.0 32.0 37.0 5 34.70225094669701 37.0 35.0 37.0 32.0 37.0 6 34.75449219852204 37.0 35.0 37.0 32.0 37.0 7 34.64522665019021 37.0 35.0 37.0 32.0 37.0 8 34.60842194976899 37.0 35.0 37.0 32.0 37.0 9 36.15451202236477 39.0 37.0 39.0 31.0 39.0 10-11 36.069161878316336 39.0 37.0 39.0 31.0 39.0 12-13 35.97638231510649 39.0 37.0 39.0 30.0 39.0 14-15 37.251256715869346 40.0 38.0 41.0 31.0 41.0 16-17 37.25492593551897 40.0 38.0 41.0 31.0 41.0 18-19 37.256947207804515 40.0 38.0 41.0 31.0 41.0 20-21 37.27611972154013 40.0 38.0 41.0 31.0 41.0 22-23 37.23531263068181 40.0 37.5 41.0 31.0 41.0 24-25 37.24225549749887 40.0 37.5 41.0 31.0 41.0 26-27 37.20997862787175 40.0 37.0 41.0 31.0 41.0 28-29 37.16730889164461 40.0 37.0 41.0 31.0 41.0 30-31 37.12485511954356 40.0 37.0 41.0 31.0 41.0 32-33 37.03415488932652 40.0 37.0 41.0 30.0 41.0 34-35 37.012860537095904 40.0 37.0 41.0 30.0 41.0 36-37 36.96369451313655 40.0 37.0 41.0 30.0 41.0 38-39 36.88893258012378 40.0 37.0 41.0 30.0 41.0 40-41 36.8204216690518 40.0 37.0 41.0 30.0 41.0 42-43 36.720833628761646 40.0 37.0 41.0 30.0 41.0 44-45 36.630169211691154 40.0 36.5 41.0 30.0 41.0 46-47 36.53002516325731 40.0 36.0 41.0 30.0 41.0 48-49 36.434203110462214 40.0 36.0 41.0 30.0 41.0 50-51 36.32160006627241 40.0 36.0 41.0 29.0 41.0 52-53 36.18951539386503 40.0 35.5 41.0 29.0 41.0 54-55 36.0220357567137 39.5 35.0 41.0 29.0 41.0 56-57 35.84521955991069 39.0 35.0 41.0 28.0 41.0 58-59 35.64654305463712 39.0 35.0 41.0 28.0 41.0 60-61 35.41285648550762 39.0 35.0 41.0 28.0 41.0 62-63 35.164119893732625 38.0 34.5 40.0 28.0 41.0 64-65 34.89004115979609 38.0 34.0 40.0 27.0 41.0 66-67 34.58755030119218 37.0 34.0 40.0 26.5 41.0 68-69 34.26407293871828 37.0 34.0 39.5 26.0 41.0 70-71 33.919354762859086 36.0 34.0 39.0 26.0 41.0 72-73 33.564335966383396 36.0 33.5 39.0 26.0 40.5 74-75 33.28179212602755 35.0 33.0 38.0 26.0 40.0 76-77 33.04391487612992 35.0 33.0 37.0 26.0 39.0 78-79 32.73438564898486 35.0 33.0 37.0 26.0 39.0 80-81 32.512327500010855 35.0 33.0 36.5 26.0 39.0 82-83 32.39663294925241 35.0 33.0 36.0 26.0 37.5 84-85 32.17226069221386 35.0 33.0 36.0 26.0 37.0 86-87 31.96608929121804 35.0 33.0 35.0 26.0 37.0 88-89 31.781342110327643 35.0 33.0 35.0 25.0 36.0 90-91 31.633497302076293 35.0 33.0 35.0 25.0 36.0 92-93 31.51184112854098 35.0 33.0 35.0 25.0 36.0 94-95 31.406660449407962 35.0 33.0 35.0 25.0 36.0 96-97 31.318426804439962 35.0 33.0 35.0 24.0 35.5 98-99 31.24191889009633 35.0 33.0 35.0 24.0 35.0 100-101 31.167737021532744 35.0 33.0 35.0 23.5 35.0 102-103 30.07622140917135 34.0 30.5 35.0 19.5 35.0 104-105 30.89219555569704 35.0 32.0 35.0 22.5 35.0 106-107 30.984548978638003 35.0 33.0 35.0 23.0 35.0 108-109 30.95941845961507 35.0 33.0 35.0 20.0 35.0 110-111 30.8920450681318 35.0 33.0 35.0 19.5 35.0 112-113 30.811264356007275 35.0 33.0 35.0 19.0 35.0 114-115 30.70794885448576 35.0 33.0 35.0 18.5 35.0 116-117 30.609785743328988 35.0 33.0 35.0 16.5 35.0 118-119 30.4781961243664 35.0 32.0 35.0 7.5 35.0 120-121 30.336279477113592 35.0 32.0 35.0 2.0 35.0 122-123 30.171077592256836 35.0 32.0 35.0 2.0 35.0 124-125 29.333998288203944 34.5 30.5 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.454906860725675 1101 2 0.4687620614887571 1101 3 0.5233714442675144 1101 4 0.5713760716901248 1101 5 0.5470046885988253 1101 6 0.48865054106619255 1101 7 0.4837277807728668 1101 8 0.4361024886546758 1101 9 0.45100918107861787 1101 10-11 0.5277412746726711 1101 12-13 0.6346062803374224 1101 14-15 0.6750129110768981 1101 16-17 0.6431234769850178 1101 18-19 0.6553846700003803 1101 20-21 0.6235754773501654 1101 22-23 0.6788363527976955 1101 24-25 0.630250181472185 1101 26-27 0.6261207713877539 1101 28-29 0.727268893182746 1101 30-31 0.6563629542765241 1101 32-33 0.7472766892324074 1101 34-35 0.7805939270700577 1101 36-37 0.7378289567537024 1101 38-39 0.718252512721314 1101 40-41 0.7865250409045075 1101 42-43 0.7256126359815411 1101 44-45 0.7247198727477553 1101 46-47 0.6783848630406126 1101 48-49 0.6104111534452983 1101 50-51 0.6302184006198672 1101 52-53 0.588531424665625 1101 54-55 0.6962213071052545 1101 56-57 0.6418257371210814 1101 58-59 0.5901551713246818 1101 60-61 0.635623890661627 1101 62-63 0.6440388896859446 1101 64-65 0.6972057022578966 1101 66-67 0.6380373125307628 1101 68-69 0.6402905030874138 1101 70-71 0.6556463871072751 1101 72-73 0.6254202239671542 1101 74-75 0.6867957593423739 1101 76-77 0.6382671332758818 1101 78-79 0.6143166290120448 1101 80-81 0.5453764404139321 1101 82-83 0.513595408235048 1101 84-85 0.5659430987687486 1101 86-87 0.5299908615972484 1101 88-89 0.5811630180265261 1101 90-91 0.5975557297674712 1101 92-93 0.5746788498433872 1101 94-95 0.638258341002075 1101 96-97 0.5282888583925036 1101 98-99 0.5552079137209773 1101 100-101 0.5308292751762238 1101 102-103 0.5559546934818265 1101 104-105 0.5417260668513393 1101 106-107 0.5466004434249072 1101 108-109 0.5617833752050032 1101 110-111 0.556141480530016 1101 112-113 0.5696403803110037 1101 114-115 0.5840238356396874 1101 116-117 0.6040317025827342 1101 118-119 0.564426208688861 1101 120-121 0.5314083584251641 1101 122-123 0.5710342755647808 1101 124-125 0.6351437178582984 1102 1 0.46537268196838255 1102 2 0.5036310057185496 1102 3 0.4784236704925 1102 4 0.4741119148830677 1102 5 0.5094888964824591 1102 6 0.5166157896135459 1102 7 0.5363695485183086 1102 8 0.4914025490623928 1102 9 0.6248247031043661 1102 10-11 0.5641406228318218 1102 12-13 0.602276280457346 1102 14-15 0.6681992821266363 1102 16-17 0.7653839066304258 1102 18-19 0.7608137306844114 1102 20-21 0.7378368914897848 1102 22-23 0.7569913563349289 1102 24-25 0.7659272973205233 1102 26-27 0.8359449059724753 1102 28-29 0.8703687679050702 1102 30-31 0.8392168965969304 1102 32-33 0.8736312743169776 1102 34-35 0.8316713218178435 1102 36-37 0.7660298885410768 1102 38-39 0.8567883569978463 1102 40-41 0.8107048385742672 1102 42-43 0.8359021650649439 1102 44-45 0.8889789766254665 1102 46-47 0.957050084418789 1102 48-49 0.9455568299164625 1102 50-51 0.9385874719901537 1102 52-53 0.9167353272245649 1102 54-55 0.9310734920438151 1102 56-57 0.9933293158354815 1102 58-59 0.8875538772979894 1102 60-61 0.9146083482300824 1102 62-63 0.9394396604672295 1102 64-65 0.9447461864778361 1102 66-67 0.9476757495420998 1102 68-69 0.9660397143262571 1102 70-71 0.974399370434611 1102 72-73 1.0271933971572267 1102 74-75 0.9322443455442766 1102 76-77 0.8952343383319814 1102 78-79 0.8903888781764948 1102 80-81 0.8882323953103111 1102 82-83 0.808802165717637 1102 84-85 0.7826224104921096 1102 86-87 0.8561625859517719 1102 88-89 0.8846457789374362 1102 90-91 0.8717584626459782 1102 92-93 0.8598359680464327 1102 94-95 0.858378999376189 1102 96-97 0.8639589247460293 1102 98-99 0.9190248535596268 1102 100-101 0.947836540599873 1102 102-103 0.9566347384013518 1102 104-105 0.9393337069452876 1102 106-107 0.9315330857454143 1102 108-109 0.9018331685020087 1102 110-111 0.8939624950740708 1102 112-113 0.9263888927488857 1102 114-115 1.0200902138777153 1102 116-117 1.0268095700180204 1102 118-119 0.9221890752496549 1102 120-121 0.9599280637416712 1102 122-123 0.9695501925387795 1102 124-125 0.9764989156645072 1103 1 0.30260588239100983 1103 2 0.407187595743995 1103 3 0.3932032553361928 1103 4 0.4254068447677568 1103 5 0.4372754083734023 1103 6 0.4431200665710264 1103 7 0.3937595203740969 1103 8 0.3875637094497222 1103 9 0.49377099822714143 1103 10-11 0.5086904813930104 1103 12-13 0.5232370355632128 1103 14-15 0.5899432663795068 1103 16-17 0.6063018129114752 1103 18-19 0.5605097695904746 1103 20-21 0.5922216140056662 1103 22-23 0.6852173019443129 1103 24-25 0.6131959987481324 1103 26-27 0.6514076855417628 1103 28-29 0.7080934976999842 1103 30-31 0.6621632078687654 1103 32-33 0.47963713762001703 1103 34-35 0.6934490516136549 1103 36-37 0.7086161715939241 1103 38-39 0.7661835180831744 1103 40-41 0.7293617516107105 1103 42-43 0.7084517329431534 1103 44-45 0.6727842860159399 1103 46-47 0.6630702725313569 1103 48-49 0.6941313917462608 1103 50-51 0.6727828558128053 1103 52-53 0.6280387981647877 1103 54-55 0.7021595668037719 1103 56-57 0.7109563677747275 1103 58-59 0.7317326664717854 1103 60-61 0.7180904432648774 1103 62-63 0.7843227278545442 1103 64-65 0.6634572586584326 1103 66-67 0.6807881000100764 1103 68-69 0.7683995098792238 1103 70-71 0.7322772829838868 1103 72-73 0.7217642943478637 1103 74-75 0.6365229478376193 1103 76-77 0.6176129206184484 1103 78-79 0.6462373031502295 1103 80-81 0.56345310653262 1103 82-83 0.5820839137870024 1103 84-85 0.5487438172900809 1103 86-87 0.5383137237573479 1103 88-89 0.5838074037596037 1103 90-91 0.499380403708507 1103 92-93 0.47099382834455383 1103 94-95 0.48626826710328785 1103 96-97 0.4868256570655767 1103 98-99 0.5476690596825691 1103 100-101 0.5670581621503672 1103 102-103 0.5806854057610344 1103 104-105 0.623798346347364 1103 106-107 0.6813593189275942 1103 108-109 0.6897397097363722 1103 110-111 0.6521901841832403 1103 112-113 0.6607191510829189 1103 114-115 0.7124942369062666 1103 116-117 0.6878925315991182 1103 118-119 0.7651858433453427 1103 120-121 0.8154112808367024 1103 122-123 0.8496521648467414 1103 124-125 0.8800852020069811 1104 1 0.221191409853148 1104 2 0.30052383702885166 1104 3 0.20690615407210444 1104 4 0.15967695667936255 1104 5 0.2223732410008452 1104 6 0.1863299804011831 1104 7 0.2786677166155229 1104 8 0.27614150044428953 1104 9 0.3376841058476785 1104 10-11 0.27107382452277307 1104 12-13 0.2880144146431576 1104 14-15 0.3887448399259057 1104 16-17 0.3917542336359148 1104 18-19 0.3620172772165873 1104 20-21 0.38702458964160513 1104 22-23 0.4486400441387275 1104 24-25 0.5439405872865493 1104 26-27 0.41585164726367907 1104 28-29 0.4975782326969309 1104 30-31 0.42768722576717977 1104 32-33 -0.2317339015060682 1104 34-35 0.33301829532167915 1104 36-37 0.4460786508878414 1104 38-39 0.5387452239626569 1104 40-41 0.5413134614165074 1104 42-43 0.5820298956598862 1104 44-45 0.5311939191958572 1104 46-47 0.6207255420415478 1104 48-49 0.5834723457711988 1104 50-51 0.6049602590546925 1104 52-53 0.5389668490163828 1104 54-55 0.5692294842582228 1104 56-57 0.49736379501592864 1104 58-59 0.5552682553842416 1104 60-61 0.5259196961730268 1104 62-63 0.6550639825097875 1104 64-65 0.6885206547121356 1104 66-67 0.7498095007521215 1104 68-69 0.6666251754361525 1104 70-71 0.654817818124414 1104 72-73 0.6499424607818156 1104 74-75 0.6872238474052565 1104 76-77 0.6671108633685847 1104 78-79 0.631496341226196 1104 80-81 0.5207749586847754 1104 82-83 0.47368662294461217 1104 84-85 0.5428299790031659 1104 86-87 0.49918584203933136 1104 88-89 0.5110042527172745 1104 90-91 0.5161334570576059 1104 92-93 0.4843426326769773 1104 94-95 0.4898045065295342 1104 96-97 0.48154142319657467 1104 98-99 0.500470102363245 1104 100-101 0.533785456315023 1104 102-103 0.5922989355185528 1104 104-105 0.5796999875007245 1104 106-107 0.5476265778715756 1104 108-109 0.5380709676209499 1104 110-111 0.4749920574306259 1104 112-113 0.4786647356044078 1104 114-115 0.5265013089204658 1104 116-117 0.5880093917549161 1104 118-119 0.5993301795773363 1104 120-121 0.7062667994007406 1104 122-123 0.7705800937269949 1104 124-125 0.9152475632791095 1105 1 0.2941494502754445 1105 2 0.33180055468187675 1105 3 0.296926602788254 1105 4 0.32468368544186177 1105 5 0.24557279634543505 1105 6 0.30575273867355435 1105 7 0.22484486792036762 1105 8 0.28988597045122333 1105 9 0.17402111533056086 1105 10-11 0.3379141698420369 1105 12-13 0.3322435684857368 1105 14-15 0.4214586709283026 1105 16-17 0.4233649147579541 1105 18-19 0.44956249436247475 1105 20-21 0.40213138658950953 1105 22-23 0.31530174806488986 1105 24-25 0.39118412544160464 1105 26-27 0.42032617073699186 1105 28-29 0.45873562818932356 1105 30-31 0.42674475588086835 1105 32-33 0.055627937870106337 1105 34-35 0.40800808592663884 1105 36-37 0.4332159353683167 1105 38-39 0.4508051840735021 1105 40-41 0.4447441231648597 1105 42-43 0.500735429543333 1105 44-45 0.5042445693052713 1105 46-47 0.47068979189386795 1105 48-49 0.4818493569759781 1105 50-51 0.49428520856019276 1105 52-53 0.5164650891330638 1105 54-55 0.5119868623014128 1105 56-57 0.5096019764038431 1105 58-59 0.4886189765335516 1105 60-61 0.5453460628392506 1105 62-63 0.566124929720651 1105 64-65 0.5797998343601947 1105 66-67 0.5457149751057102 1105 68-69 0.5474912613221363 1105 70-71 0.581787379624565 1105 72-73 0.4548746459975064 1105 74-75 0.4845725066481066 1105 76-77 0.5542477852831738 1105 78-79 0.5755491153962282 1105 80-81 0.4685959434459548 1105 82-83 0.42560788261130256 1105 84-85 0.3713907846406883 1105 86-87 0.375715985191853 1105 88-89 0.3748729907439632 1105 90-91 0.3641633315660684 1105 92-93 0.4169046003669443 1105 94-95 0.38336459969631065 1105 96-97 0.37750656553649264 1105 98-99 0.33405097803698425 1105 100-101 0.3944586652050006 1105 102-103 0.34539524821195045 1105 104-105 0.38512580905200977 1105 106-107 0.4499588088511395 1105 108-109 0.3744303065585939 1105 110-111 0.3697382302311425 1105 112-113 0.35294898449910406 1105 114-115 0.42806605121784 1105 116-117 0.5148445161253754 1105 118-119 0.4315097380894848 1105 120-121 0.4371790289898918 1105 122-123 0.3746547760847463 1105 124-125 0.41285716061688404 1106 1 0.23111075276191784 1106 2 0.2792379251064716 1106 3 0.29114660317477004 1106 4 0.30372684868033417 1106 5 0.23445676961724615 1106 6 0.32728656557662816 1106 7 0.36044085921432867 1106 8 0.24521937725765497 1106 9 0.3462191279935851 1106 10-11 0.4019687808968868 1106 12-13 0.38330693485236367 1106 14-15 0.42021078901082376 1106 16-17 0.3992319045437469 1106 18-19 0.4535405750049435 1106 20-21 0.517307428649552 1106 22-23 0.49132900902095145 1106 24-25 0.4432912624475094 1106 26-27 0.4272371685277605 1106 28-29 0.42767824297377643 1106 30-31 0.48082873121874314 1106 32-33 0.4224772194210402 1106 34-35 0.4743865302899479 1106 36-37 0.44111313246256856 1106 38-39 0.4392496245715236 1106 40-41 0.44963310189288563 1106 42-43 0.4996699245640812 1106 44-45 0.38813012027543436 1106 46-47 0.4979797077346717 1106 48-49 0.5106144512429438 1106 50-51 0.43318025793023196 1106 52-53 0.5006813444809737 1106 54-55 0.5114619591294982 1106 56-57 0.6141185579641686 1106 58-59 0.6247307303891603 1106 60-61 0.6207946332100747 1106 62-63 0.5772470501890226 1106 64-65 0.6271796798774929 1106 66-67 0.6215690403587502 1106 68-69 0.6512331253810046 1106 70-71 0.6376287606123441 1106 72-73 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98-99 -2.52688940569233 2216 100-101 -2.6301710094799162 2216 102-103 -2.7051825745315377 2216 104-105 -2.5708728045125895 2216 106-107 -2.5177683598086347 2216 108-109 -2.6142067635188653 2216 110-111 -2.5891962225129745 2216 112-113 -2.537353579237312 2216 114-115 -2.4909115961740866 2216 116-117 -2.60498751606168 2216 118-119 -2.7571362179791947 2216 120-121 -2.784062308360319 2216 122-123 -2.8337099521597153 2216 124-125 -3.0762942533119073 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 52972.0 3 9094.0 4 5075.0 5 4262.0 6 4487.0 7 5123.0 8 6062.0 9 6790.0 10 7696.0 11 8266.0 12 8903.0 13 9059.0 14 9161.0 15 8261.0 16 7555.0 17 7073.0 18 6901.0 19 6694.0 20 6977.0 21 7776.0 22 9121.0 23 12066.0 24 15592.0 25 20266.0 26 25139.0 27 28811.0 28 35479.0 29 44111.0 30 53054.0 31 63371.0 32 81573.0 33 108728.0 34 149328.0 35 218729.0 36 408903.0 37 853073.0 38 446112.0 39 2705.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.95172325812399 18.653537288647506 12.817317229455544 24.577422223772956 2 15.515696827344893 19.626058517804324 40.33330341142467 24.524941243426117 3 19.022389906571536 23.957683363671812 29.51487627242634 27.505050457330317 4 12.563625587379503 15.812577834257139 37.50601869802798 34.11777788033538 5 13.553773972328928 37.789694033674834 35.02742031029107 13.629111683705176 6 32.94570824518163 36.170697099702764 17.339165103632954 13.54442955148266 7 28.60277816059311 32.100514418966924 21.19841038103075 18.098297039409214 8 25.69364667394706 36.299930432594344 19.822783125340244 18.183639768118347 9 25.905247703672778 16.02866767743735 20.128216831041158 37.93786778784871 10-11 24.82998814209121 26.449374731742093 28.24868508158678 20.471952044579915 12-13 25.057220807566388 23.90465623863223 28.18654056020371 22.85158239359767 14-15 22.199709664379224 25.802700888664127 25.481124093110342 26.516465353846307 16-17 22.49848530803343 28.938180929500817 26.177313878360597 22.386019884105153 18-19 22.370871566348363 28.239956485423217 27.394579263616535 21.994592684611884 20-21 23.082687924952953 27.48081233073715 27.399213361935708 22.037286382374184 22-23 22.809020397963444 27.415049104322176 27.082636905192775 22.693293592521606 24-25 22.587412205882888 27.533562722554223 27.23428980878978 22.64473526277311 26-27 22.552142177963987 27.748263261162307 27.172060275331944 22.527534285541762 28-29 22.4174336683556 27.755605403997862 27.291494827739204 22.535466099907335 30-31 22.579293594428364 27.647038712899125 27.293574061000974 22.480093631671537 32-33 22.34433886434675 27.655733877051038 27.309539379646814 22.690387878955402 34-35 22.74642576507876 27.66283888606111 27.140963035339638 22.44977231352049 36-37 22.73165055616272 27.46003787420957 27.11535675786006 22.692954811767653 38-39 22.4380400442174 27.65406136508497 27.23330927685949 22.67458931383814 40-41 22.79303763751498 27.520565925428485 27.175729191876684 22.510667245179842 42-43 22.57113969612852 27.522498162859005 27.203587930910416 22.70277421010206 44-45 22.574766684620993 27.664272998801348 27.1443062615531 22.616654055024558 46-47 22.5766224287832 27.613932979670153 27.002948970113938 22.80649562143271 48-49 22.511574799843615 27.627297352168544 27.16163351674527 22.699494331242573 50-51 22.53979993948574 27.71956636897163 27.04863947401657 22.69199421752606 52-53 22.586426972760755 27.63662327296887 27.023098802863128 22.753850951407248 54-55 22.60360756021701 27.46497747659759 27.18162469766346 22.74979026552194 56-57 22.534792388413916 27.716565960423985 27.132621755418906 22.616019895743193 58-59 22.57829760859291 27.56369826336023 27.101305409871006 22.75669871817585 60-61 22.63888573435074 27.463221365903916 27.281083885175615 22.61680901456973 62-63 22.450689712511508 27.52992873440185 27.255770169127448 22.763611383959198 64-65 22.409977096627227 27.540001850006625 27.157515706429024 22.892505346937124 66-67 22.45800416268948 27.65598635603281 26.956867528913826 22.929141952363885 68-69 22.386824813113535 27.570425044022468 27.1397428011813 22.9030073416827 70-71 22.281314681918545 27.414555344639442 27.225585086235288 23.078544887206732 72-73 22.400061803854562 27.32771948321071 27.22472517734525 23.047493535589478 74-75 22.256111802292136 27.530805519229425 27.15844019880568 23.054642479672765 76-77 22.31079802922717 27.349652962811366 27.250725372064434 23.08882363589703 78-79 22.331316557466295 27.328103712630796 27.300910412071204 23.0396693178317 80-81 22.227715393927056 27.485232052172204 27.2144106089302 23.072641944970538 82-83 22.268919171422194 27.49756982791401 27.187827400708294 23.045683599955506 84-85 22.38447253592333 27.250582401681488 27.236696004407296 23.128249057987887 86-87 22.28675446824264 27.203089018090697 27.37352587476108 23.136630638905583 88-89 22.306658715758736 27.17865570163521 27.359568542684727 23.155117039921326 90-91 22.324131846485 27.23801839570482 27.33967360935068 23.098176148459498 92-93 22.24779865870601 27.046872600694726 27.460036944190286 23.24529179640898 94-95 22.045804550654033 27.093736059158687 27.464068404272812 23.39639098591447 96-97 22.151473136474227 27.026122492437498 27.4151808904428 23.407223480645467 98-99 22.16874332295762 26.86616637055501 27.382058140079764 23.58303216640761 100-101 22.02404152289858 26.97727958758977 27.536926255009558 23.46175263450209 102-103 22.12462921376065 26.768941379181342 27.58584243977436 23.52058696728364 104-105 21.98508246054044 26.79639756132554 27.571277834178126 23.64724214395589 106-107 21.89740033931832 26.83195389164551 27.59026241415795 23.680383354878217 108-109 21.93110139827116 26.817294796478386 27.444177405925835 23.807426399324623 110-111 21.812300921241977 26.914094626188383 27.532815918561404 23.740788534008235 112-113 21.879844968281866 26.899064061585392 27.593106051825238 23.627984918307497 114-115 21.77611417007432 26.93634793097623 27.472482955691156 23.8150549432583 116-117 21.65916815982864 26.911374479209808 27.6163576438994 23.813099717062155 118-119 21.719170526843754 26.88764022483222 27.496588082002337 23.896601166321688 120-121 21.710790759271656 26.95507417979196 27.543831531081665 23.79030352985472 122-123 21.72796843066479 27.005412628206376 27.492216747086196 23.77440219404264 124-125 21.69190461259084 26.883635368755478 27.45005851531335 23.97440150334033 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 11443.0 1 6069.5 2 665.0 3 618.0 4 553.5 5 469.5 6 385.5 7 357.0 8 381.0 9 386.5 10 373.5 11 357.5 12 393.0 13 414.5 14 458.5 15 547.0 16 643.0 17 732.5 18 910.5 19 1223.5 20 1602.0 21 2252.0 22 3185.5 23 4563.5 24 6730.0 25 9438.0 26 12922.0 27 17202.0 28 21371.0 29 26082.0 30 31865.0 31 37118.0 32 42232.5 33 47958.5 34 54531.5 35 60298.5 36 66748.5 37 74234.0 38 78989.0 39 82253.5 40 85759.5 41 89669.5 42 94195.0 43 99422.5 44 104502.0 45 108373.0 46 109348.0 47 110452.5 48 110852.5 49 110228.5 50 107917.0 51 102188.0 52 97537.0 53 94055.5 54 89916.0 55 83202.5 56 75160.5 57 67611.0 58 61418.5 59 54842.0 60 46144.5 61 37312.0 62 29843.0 63 23096.5 64 17423.0 65 13406.0 66 10090.0 67 7703.5 68 5605.0 69 3688.5 70 2593.0 71 1968.5 72 1528.5 73 1186.5 74 878.5 75 735.5 76 523.5 77 259.5 78 134.5 79 92.5 80 54.5 81 28.5 82 22.0 83 19.0 84 14.5 85 10.0 86 11.5 87 13.0 88 10.5 89 10.0 90 10.0 91 9.0 92 5.0 93 3.0 94 5.0 95 5.0 96 3.0 97 3.5 98 5.0 99 3.0 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4688628204553117 2 0.4609043434473518 3 0.45641865640650164 4 0.4524394179025217 5 0.4607958187608796 6 0.5192544498738942 7 0.5156731352203123 8 0.5244274599290683 9 0.5292748959248257 10-11 0.5472538189837169 12-13 0.5552122959916769 14-15 0.5343574687412728 16-17 0.5286599227014833 18-19 0.539892227751354 20-21 0.5399464900945901 22-23 0.54989458635454 24-25 0.543274580479737 26-27 0.5509436583237711 28-29 0.5310474658038713 30-31 0.5372333729327856 32-33 0.5383547946929981 34-35 0.5369620612166052 36-37 0.4847255121279955 38-39 0.4518244446791793 40-41 0.4509562471874019 42-43 0.4626769133263974 44-45 0.46745199953117333 46-47 0.4720281238107503 48-49 0.4860639832611524 50-51 0.4677594861428445 52-53 0.4684648966049137 54-55 0.4577752149874039 56-57 0.4413879873301046 58-59 0.45882428695663496 60-61 0.4550982727210901 62-63 0.47101522673700996 64-65 0.4705087782001398 66-67 0.47902796608820597 68-69 0.48763759121499894 70-71 0.48423715103887066 72-73 0.49597590462561153 74-75 0.48548518493330073 76-77 0.48279015521924157 78-79 0.49452890880598244 80-81 0.486841743514203 82-83 0.48886753766168367 84-85 0.48671513137998546 86-87 0.4768755598065077 88-89 0.4811080225789227 90-91 0.4663124903232154 92-93 0.46667423927812274 94-95 0.46179062838687457 96-97 0.4502870116208234 98-99 0.4140216788913697 100-101 0.4109829876701486 102-103 0.40070931735078213 104-105 0.40562910313752104 106-107 0.4044715064818178 108-109 0.4167347960531742 110-111 0.4391451438096795 112-113 0.4390547065709527 114-115 0.4327602747555662 116-117 0.4268637667905777 118-119 0.4689351702462931 120-121 0.4626769133263974 122-123 0.4269903789247953 124-125 0.4368842128415091 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2764348.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 73.31641132932238 #Duplication Level Percentage of deduplicated Percentage of total 1 84.04186178797927 61.616477077295485 2 10.114945047843719 14.83182943402405 3 2.9268083224729486 6.437492485575322 4 1.168580952932703 3.4270464726730223 5 0.5818017282509561 2.1327807410278865 6 0.32400701367159257 1.4253018892759115 7 0.193163051733664 0.9913415228172742 8 0.12562074444123994 0.7368049736759709 9 0.09133992301030766 0.6027043829591118 >10 0.40406369541727283 5.095795235717772 >50 0.020547872880919103 1.04810155736367 >100 0.007055365970503478 0.7600833323619215 >500 1.022466974744704E-4 0.04355385699808877 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 1.022466974744704E-4 0.8506870382346152 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 11348 0.4105127140287692 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 3.617489549072693E-5 0.0 8 0.0 0.0 0.0 3.617489549072693E-5 0.0 9 0.0 0.0 0.0 3.617489549072693E-5 0.0 10-11 0.0 0.0 0.0 3.617489549072693E-5 0.0 12-13 0.0 0.0 0.0 3.617489549072693E-5 0.0 14-15 0.0 0.0 0.0 7.234979098145386E-5 0.0 16-17 0.0 0.0 0.0 7.234979098145386E-5 0.0 18-19 0.0 0.0 0.0 7.234979098145386E-5 0.0 20-21 0.0 0.0 0.0 1.0852468647218078E-4 0.0 22-23 0.0 0.0 0.0 1.0852468647218078E-4 0.0 24-25 0.0 0.0 0.0 1.4469958196290772E-4 0.0 26-27 0.0 0.0 0.0 3.436615071619058E-4 0.0 28-29 0.0 0.0 0.0 0.0013746460286476233 0.0 30-31 0.0 0.0 0.0 0.005299622189391495 0.0 32-33 0.0 0.0 0.0 0.015139193762869218 0.0 34-35 0.0 0.0 0.0 0.030567786689664252 0.0 36-37 0.0 0.0 0.0 0.05744573403927436 0.0 38-39 0.0 0.0 0.0 0.09684019522867598 0.0 40-41 0.0 0.0 0.0 0.13856793717722948 0.0 42-43 0.0 0.0 0.0 0.1829726213920968 0.0 44-45 0.0 0.0 0.0 0.2400023441332278 0.0 46-47 0.0 0.0 0.0 0.30907830707277084 0.0 48-49 0.0 0.0 0.0 0.3831644930377796 0.0 50-51 0.0 0.0 0.0 0.4638706848775914 0.0 52-53 0.0 0.0 0.0 0.5444321771354403 0.0 54-55 0.0 0.0 0.0 0.6375101832330807 0.0 56-57 0.0 0.0 0.0 0.7514068416856343 0.0 58-59 0.0 3.617489549072693E-5 0.0 0.8784530746490673 0.0 60-61 0.0 3.617489549072693E-5 0.0 1.0175455478109123 0.0 62-63 0.0 3.617489549072693E-5 0.0 1.1576328305987524 0.0 64-65 0.0 3.617489549072693E-5 0.0 1.306456350647603 0.0 66-67 0.0 3.617489549072693E-5 0.0 1.4894289720396998 0.0 68-69 0.0 3.617489549072693E-5 0.0 1.6972175717384352 0.0 70-71 0.0 3.617489549072693E-5 0.0 1.9289358648042865 0.0 72-73 0.0 3.617489549072693E-5 0.0 2.167274163744941 0.0 74-75 0.0 3.617489549072693E-5 0.0 2.4112919212776394 0.0 76-77 0.0 3.617489549072693E-5 0.0 2.6824227629806376 0.0 78-79 0.0 7.234979098145386E-5 0.0 2.994159924871977 0.0 80-81 0.0 7.234979098145386E-5 0.0 3.3424879935521865 0.0 82-83 0.0 7.234979098145386E-5 0.0 3.7054849823538856 0.0 84-85 0.0 9.043723872681733E-5 0.0 4.071593012167788 0.0 86-87 0.0 1.4469958196290772E-4 0.0 4.457940896008751 0.0 88-89 0.0 1.4469958196290772E-4 0.0 4.892546090434345 0.0 90-91 0.0 1.4469958196290772E-4 0.0 5.370253672837139 0.0 92-93 0.0 1.4469958196290772E-4 0.0 5.87587018711103 0.0 94-95 0.0 1.4469958196290772E-4 0.0 6.376187079195528 0.0 96-97 0.0 1.4469958196290772E-4 0.0 6.88636163030125 0.0 98-99 0.0 1.4469958196290772E-4 0.0 7.43848097272847 0.0 100-101 0.0 1.4469958196290772E-4 0.0 8.046454353793372 0.0 102-103 0.0 1.4469958196290772E-4 0.0 8.68705749059091 0.0 104-105 0.0 1.4469958196290772E-4 0.0 9.321963081348658 0.0 106-107 0.0 1.4469958196290772E-4 0.0 9.94757172396529 0.0 108-109 0.0 1.4469958196290772E-4 0.0 10.604941924822779 0.0 110-111 0.0 1.4469958196290772E-4 0.0 11.321494254703097 0.0 112-113 0.0 1.4469958196290772E-4 0.0 12.075216289700139 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2635 0.0 48.786037 1 GTATCAA 4170 0.0 44.386074 1 ATCAACG 4255 0.0 41.544247 3 TCAACGC 4280 0.0 41.169254 4 CAACGCA 4395 0.0 40.228924 5 AACGCAG 4775 0.0 37.152805 6 TATCAAC 5220 0.0 34.089073 2 GTACATG 9285 0.0 33.907528 1 TACATGG 9875 0.0 30.736017 2 ACATGGG 9550 0.0 30.16449 3 ACGCAGA 5855 0.0 29.690704 7 CGCAGAG 6030 0.0 28.434116 8 GAGTACA 7095 0.0 23.906437 1 AGTACAT 7085 0.0 22.595758 2 CATGGGA 5885 0.0 22.557108 4 CATGGGG 5025 0.0 22.1529 4 GCAGAGT 7550 0.0 21.999956 9 CAGAGTA 7065 0.0 19.46527 10-11 GTACGGT 185 6.943927E-4 19.303387 3 AGAGTAC 6430 0.0 19.165491 10-11 >>END_MODULE