FastQCFastQC Report
Thu 26 May 2016
SRR1364125_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364125_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2764348
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60150.21759199637672247No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58280.21082729091995656No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33280.12039005219313921No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA47650.069.293561
GTATCAA81150.047.738721
ATCAACG97450.039.5000723
TCAACGC99250.038.7836954
CAACGCA101600.037.8280755
TATCAAC104350.036.8331682
AACGCAG106100.036.505376
GTGGTAT24150.033.3167461
GTACATG93400.031.6505131
TGGTATC24700.030.8327332
ACGCAGA126250.030.6845197
CGCAGAG126600.030.505688
TACATGG96650.029.3640212
ACATGGG94250.028.7213923
GCAGAGT140150.026.961899
GAGTACT94650.026.09186212-13
CAGAGTA137850.024.9518410-11
AGAGTAC131450.023.85786210-11
GTACTTT104800.023.44660214-15
CATGGGG47450.022.3182054
Produced by FastQC (version 0.11.5)