FastQCFastQC Report
Thu 26 May 2016
SRR1364124_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364124_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1478824
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN60300.40775643349039503No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20850.061.9354971
GTATCAA36700.048.483181
ATCAACG43350.039.5464483
TCAACGC43750.039.327634
GTACATG100100.038.820851
CAACGCA44550.038.49175
TACATGG101150.037.7170032
AACGCAG46550.036.8379176
TATCAAC48900.036.026982
ACATGGG102250.035.8609243
CATGGGA64500.031.752414
CATGGGG36300.030.669944
GTGGTAT10800.030.305741
ACGCAGA55900.030.251277
CGCAGAG57100.029.4069568
ATGGGAG27500.028.7966025
TGGTATC11950.027.8910712
GAGTACA70850.027.2139231
AGTACAT69450.027.0806982
ATACCGT1807.247945E-726.4630416
Produced by FastQC (version 0.11.5)