##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364124_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1478824 Sequences flagged as poor quality 0 Sequence length 125 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.976033659177833 31.0 31.0 34.0 28.0 34.0 2 31.012437585540944 33.0 31.0 34.0 27.0 34.0 3 31.10061575954948 33.0 31.0 34.0 27.0 34.0 4 34.50952648861528 37.0 35.0 37.0 31.0 37.0 5 34.60436874164877 37.0 35.0 37.0 32.0 37.0 6 34.61465191260082 37.0 35.0 37.0 31.0 37.0 7 34.51402195257854 37.0 35.0 37.0 31.0 37.0 8 34.43361076098305 37.0 35.0 37.0 30.0 37.0 9 35.86997370883891 39.0 37.0 39.0 30.0 39.0 10-11 35.79218825228695 39.0 37.0 39.0 30.0 39.0 12-13 35.666849807685026 39.0 37.0 39.0 30.0 39.0 14-15 36.90979961104229 40.0 37.0 41.0 30.0 41.0 16-17 36.9465365723034 40.0 37.0 41.0 30.0 41.0 18-19 36.978919060009844 40.0 37.0 41.0 30.0 41.0 20-21 37.01622573071576 40.0 37.0 41.0 30.0 41.0 22-23 37.004489378046344 40.0 37.0 41.0 30.0 41.0 24-25 37.04572281759019 40.0 37.0 41.0 30.0 41.0 26-27 37.04542900304567 40.0 37.0 41.0 30.0 41.0 28-29 37.02858352312378 40.0 37.0 41.0 30.0 41.0 30-31 36.9901790206272 40.0 37.0 41.0 30.0 41.0 32-33 36.90531936187132 40.0 37.0 41.0 30.0 41.0 34-35 36.88496974623079 40.0 37.0 41.0 30.0 41.0 36-37 36.82551304279617 40.0 37.0 41.0 30.0 41.0 38-39 36.74229725782108 40.0 37.0 41.0 30.0 41.0 40-41 36.6580894683884 40.0 36.0 41.0 30.0 41.0 42-43 36.537334395438535 40.0 36.0 41.0 30.0 41.0 44-45 36.43696038203329 40.0 36.0 41.0 30.0 41.0 46-47 36.320761970322366 40.0 36.0 41.0 29.5 41.0 48-49 36.20665948077662 40.0 35.5 41.0 29.0 41.0 50-51 36.074467955618786 40.0 35.0 41.0 29.0 41.0 52-53 35.93100565043575 39.5 35.0 41.0 28.5 41.0 54-55 35.74867969413534 39.0 35.0 41.0 28.0 41.0 56-57 35.56544693621418 39.0 35.0 41.0 28.0 41.0 58-59 35.350919717288875 39.0 35.0 41.0 28.0 41.0 60-61 35.114252608829716 38.0 34.0 41.0 27.5 41.0 62-63 34.85214805818677 38.0 34.0 40.0 27.0 41.0 64-65 34.57065850973476 37.0 34.0 40.0 26.0 41.0 66-67 34.26829054708335 37.0 34.0 40.0 26.0 41.0 68-69 33.95184213942971 36.5 34.0 39.5 26.0 41.0 70-71 33.60715304863865 36.0 33.0 39.0 26.0 41.0 72-73 33.260924220867395 35.5 33.0 39.0 25.5 40.5 74-75 32.9894243669294 35.0 33.0 38.0 25.5 40.0 76-77 32.756362149924534 35.0 33.0 37.0 25.0 39.0 78-79 32.44366232898574 35.0 33.0 37.0 25.0 39.0 80-81 32.23282588056456 35.0 33.0 36.0 25.0 39.0 82-83 32.10919791672302 35.0 33.0 36.0 25.0 37.5 84-85 31.87526710413139 35.0 33.0 36.0 25.0 37.0 86-87 31.670772857351515 35.0 33.0 35.0 24.5 37.0 88-89 31.473814666248316 35.0 33.0 35.0 24.0 36.0 90-91 31.319442678777193 35.0 33.0 35.0 23.5 36.0 92-93 31.189613165596448 35.0 33.0 35.0 23.0 36.0 94-95 31.067239914959455 35.0 33.0 35.0 22.5 36.0 96-97 30.970211465326503 35.0 33.0 35.0 20.0 35.5 98-99 30.884804412154523 35.0 33.0 35.0 20.0 35.0 100-101 30.796925800500937 35.0 32.5 35.0 19.5 35.0 102-103 29.713337422167882 34.0 30.0 35.0 17.5 35.0 104-105 30.510987784888535 35.0 32.0 35.0 18.0 35.0 106-107 30.58344062579455 35.0 32.0 35.0 17.0 35.0 108-109 30.54432745208355 35.0 32.0 35.0 15.5 35.0 110-111 30.45984376775059 35.0 32.0 35.0 9.0 35.0 112-113 30.370855828685496 35.0 32.0 35.0 4.5 35.0 114-115 30.254564099581827 35.0 32.0 35.0 2.0 35.0 116-117 30.147071592021767 35.0 32.0 35.0 2.0 35.0 118-119 30.00105962575668 35.0 32.0 35.0 2.0 35.0 120-121 29.8486310744213 35.0 32.0 35.0 2.0 35.0 122-123 29.67395714432549 35.0 32.0 35.0 2.0 35.0 124-125 28.83699750612649 34.5 30.5 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.39936811737438305 1101 2 0.4539927500218859 1101 3 0.4805829192866362 1101 4 0.4546396050720958 1101 5 0.4551816872672134 1101 6 0.3897646308650593 1101 7 0.39453093720682375 1101 8 0.39008670671830714 1101 9 0.41915172494812936 1101 10-11 0.43426778557369516 1101 12-13 0.519529886401294 1101 14-15 0.5813054563420366 1101 16-17 0.5477971782925337 1101 18-19 0.45828077630855546 1101 20-21 0.49621479445332994 1101 22-23 0.5393347552385492 1101 24-25 0.5066876941171259 1101 26-27 0.5248234785697221 1101 28-29 0.6096443732181669 1101 30-31 0.5783565678623859 1101 32-33 0.6320721549529793 1101 34-35 0.618060557911889 1101 36-37 0.5421590313148386 1101 38-39 0.5158039100838465 1101 40-41 0.5979639325036104 1101 42-43 0.47188457593477295 1101 44-45 0.5616666862351991 1101 46-47 0.6352194904653814 1101 48-49 0.5570486569564324 1101 50-51 0.6143172241116588 1101 52-53 0.612909283204786 1101 54-55 0.681341894450135 1101 56-57 0.762466266095096 1101 58-59 0.6954027761352322 1101 60-61 0.6063597547524466 1101 62-63 0.5694930830411451 1101 64-65 0.5685954511106317 1101 66-67 0.6498648870091799 1101 68-69 0.621604524168454 1101 70-71 0.7305990030220073 1101 72-73 0.8726892155158552 1101 74-75 0.788323633713901 1101 76-77 0.6866824547891852 1101 78-79 0.652122967920171 1101 80-81 0.6141934573961265 1101 82-83 0.6889317255598684 1101 84-85 0.747251619471367 1101 86-87 0.6864993457967081 1101 88-89 0.8067465848178266 1101 90-91 0.8108340842186337 1101 92-93 0.8942345032361381 1101 94-95 0.8308539196366915 1101 96-97 0.76700281450114 1101 98-99 0.7004685054647624 1101 100-101 0.6970813112789038 1101 102-103 0.7560471110470424 1101 104-105 0.8351289386074363 1101 106-107 0.8483205559028661 1101 108-109 0.8405321887396937 1101 110-111 0.8468480813324071 1101 112-113 0.8996833098019721 1101 114-115 0.8706885776027811 1101 116-117 0.817754526272676 1101 118-119 0.8902110193128685 1101 120-121 0.8657172206575083 1101 122-123 1.036120390722445 1101 124-125 1.0723561628902623 1102 1 0.4069226063733673 1102 2 0.43211113780121124 1102 3 0.5157233779059602 1102 4 0.5482288689760395 1102 5 0.4489193229521291 1102 6 0.4512408655763167 1102 7 0.5095895978182057 1102 8 0.4803997496730119 1102 9 0.5195351262314389 1102 10-11 0.5584798023977626 1102 12-13 0.5892432278198143 1102 14-15 0.5930876809726016 1102 16-17 0.6054127362564401 1102 18-19 0.6239450733273557 1102 20-21 0.6770873065381053 1102 22-23 0.6303525641529006 1102 24-25 0.6515774689360683 1102 26-27 0.6965267221058724 1102 28-29 0.7304100791238 1102 30-31 0.7600074942183781 1102 32-33 0.6668098594480014 1102 34-35 0.6872020228789495 1102 36-37 0.6659051822498014 1102 38-39 0.735511660681631 1102 40-41 0.8035445392366753 1102 42-43 0.7243164754376181 1102 44-45 0.7208311165735779 1102 46-47 0.7623592695849624 1102 48-49 0.7426812153160043 1102 50-51 0.782835527598607 1102 52-53 0.7971433837707522 1102 54-55 0.7460185514833952 1102 56-57 0.8112365005655136 1102 58-59 0.9819524441264846 1102 60-61 0.8666625622045814 1102 62-63 0.7805473183028795 1102 64-65 0.6587905492510444 1102 66-67 0.6537183601990506 1102 68-69 0.7221932805978994 1102 70-71 0.6481576206270745 1102 72-73 0.5492201327545061 1102 74-75 0.6481940126855292 1102 76-77 0.5580449808080061 1102 78-79 0.6597770165345054 1102 80-81 0.6499781505890923 1102 82-83 0.5982209817226689 1102 84-85 0.6284174346130236 1102 86-87 0.6268346445185706 1102 88-89 0.5996736590123142 1102 90-91 0.6197483253292262 1102 92-93 0.5618610708162528 1102 94-95 0.629408154898428 1102 96-97 0.6866804606796002 1102 98-99 0.6472782971421829 1102 100-101 0.6765935336731523 1102 102-103 0.807853053659418 1102 104-105 0.7489010653556427 1102 106-107 0.745635349214524 1102 108-109 0.6838120222192501 1102 110-111 0.6172078076402308 1102 112-113 0.6294189499939549 1102 114-115 0.6671043239343462 1102 116-117 0.6880541512576208 1102 118-119 0.6419736330645698 1102 120-121 0.5562650780829799 1102 122-123 0.5625855083017015 1102 124-125 0.6352344034159714 1103 1 0.17482469002510825 1103 2 0.2875291417749253 1103 3 0.2690348048762594 1103 4 0.2628061525870251 1103 5 0.25522440726615514 1103 6 0.2279831856129917 1103 7 0.11672572293919359 1103 8 0.16051526752924872 1103 9 0.18545735864266533 1103 10-11 0.19753776510599863 1103 12-13 0.18359374236461434 1103 14-15 0.2084482444246447 1103 16-17 0.2521981048437354 1103 18-19 0.27159555221064124 1103 20-21 0.32209214631598115 1103 22-23 0.3123259835833352 1103 24-25 0.3078558071091777 1103 26-27 0.3494525588779922 1103 28-29 0.3785190632798887 1103 30-31 0.3440438689700116 1103 32-33 0.15299547766594657 1103 34-35 0.32823123985382807 1103 36-37 0.28340925785222737 1103 38-39 0.3430734952121739 1103 40-41 0.34955331451814686 1103 42-43 0.32470970739034755 1103 44-45 0.3441887761588873 1103 46-47 0.31380165075567845 1103 48-49 0.17413499543724953 1103 50-51 0.2553067436564831 1103 52-53 0.15178256559356385 1103 54-55 0.23466895394913223 1103 56-57 0.2919824631718626 1103 58-59 0.4043844961923142 1103 60-61 0.26065423269212573 1103 62-63 0.3595016078744351 1103 64-65 0.39701619752655404 1103 66-67 0.4388580132633848 1103 68-69 0.29473008838228765 1103 70-71 0.28479728642767554 1103 72-73 0.24074383492985874 1103 74-75 0.2646097750779006 1103 76-77 0.21514289715626234 1103 78-79 0.2885883416147479 1103 80-81 0.29767798247048916 1103 82-83 0.23192080086781175 1103 84-85 0.06007722370059909 1103 86-87 0.12139021281037898 1103 88-89 0.131088593547954 1103 90-91 0.10657871658705886 1103 92-93 0.1075740465154773 1103 94-95 0.20453965675792318 1103 96-97 0.030191655850011045 1103 98-99 0.0502789594275761 1103 100-101 0.15328745593103932 1103 102-103 0.06283452240464271 1103 104-105 0.04534955992999201 1103 106-107 -0.014219648492947101 1103 108-109 0.051416077443661834 1103 110-111 0.07537079352845666 1103 112-113 0.10345035760514065 1103 114-115 0.1480989619699038 1103 116-117 0.183819982425792 1103 118-119 0.36005466113527973 1103 120-121 0.21777292361764822 1103 122-123 0.27117706925743335 1103 124-125 0.28224520376240037 1104 1 0.17252793502217756 1104 2 0.19451781325136608 1104 3 0.24186157143545728 1104 4 0.27313188463992333 1104 5 0.2749751712905848 1104 6 0.18346528367030146 1104 7 0.23218145845353888 1104 8 0.1889727990069332 1104 9 0.2169238687288768 1104 10-11 0.2837844796046127 1104 12-13 0.18196299798523796 1104 14-15 0.2996222293549806 1104 16-17 0.29839326619349293 1104 18-19 0.38205424475174254 1104 20-21 0.2596813630452033 1104 22-23 0.23230231528743417 1104 24-25 0.27786388380943805 1104 26-27 0.2951350883772861 1104 28-29 0.43296369499262966 1104 30-31 0.37425870194784494 1104 32-33 -0.1745878129279319 1104 34-35 0.42406156028196307 1104 36-37 0.413271966029626 1104 38-39 0.44149083649776344 1104 40-41 0.6014904841934907 1104 42-43 0.5885393198235036 1104 44-45 0.4922207374197214 1104 46-47 0.5691939204413501 1104 48-49 0.5208057578427159 1104 50-51 0.5218360399686901 1104 52-53 0.5416225961847445 1104 54-55 0.5059228480724727 1104 56-57 0.5235315525344788 1104 58-59 0.47480945627697935 1104 60-61 0.542271916618283 1104 62-63 0.526972622065145 1104 64-65 0.5365893707998666 1104 66-67 0.6187847853198178 1104 68-69 0.5685078758255315 1104 70-71 0.5564287375938477 1104 72-73 0.550714240498607 1104 74-75 0.6194097936839142 1104 76-77 0.6164295511490963 1104 78-79 0.5167983164905934 1104 80-81 0.41682048699664875 1104 82-83 0.4659901590027147 1104 84-85 0.4998052255660319 1104 86-87 0.5082200566105008 1104 88-89 0.46184336330158615 1104 90-91 0.39798449133713376 1104 92-93 0.44345966545833093 1104 94-95 0.3770466778086927 1104 96-97 0.3821267716150416 1104 98-99 0.40939218969085545 1104 100-101 0.457295480679452 1104 102-103 0.5609409983234492 1104 104-105 0.5390141527513386 1104 106-107 0.5161036188675787 1104 108-109 0.43740593233489733 1104 110-111 0.5103557727990626 1104 112-113 0.6024810931647941 1104 114-115 0.5593135539153131 1104 116-117 0.527369405246791 1104 118-119 0.44332592409077165 1104 120-121 0.5021020712025823 1104 122-123 0.5012008281670219 1104 124-125 0.6349891977359832 1105 1 0.1196758948232528 1105 2 0.13317752927008186 1105 3 0.1518766696320064 1105 4 0.09256868092420945 1105 5 0.11810679326189444 1105 6 0.1968143581312347 1105 7 0.18096452205690383 1105 8 0.11260055397134039 1105 9 0.09296481974627113 1105 10-11 0.12013240203410902 1105 12-13 0.08613936931735111 1105 14-15 0.12474750864636519 1105 16-17 0.10494997805374595 1105 18-19 0.17529393158410755 1105 20-21 0.16832192904207233 1105 22-23 0.04919982527957245 1105 24-25 0.12176867407309544 1105 26-27 0.19064344774350417 1105 28-29 0.21593260684833382 1105 30-31 0.22153140202711086 1105 32-33 -0.0957908826032039 1105 34-35 0.19484838551756667 1105 36-37 0.27400979836524897 1105 38-39 0.2998390436816152 1105 40-41 0.2012269601582588 1105 42-43 0.3032369178836021 1105 44-45 0.3170137709218679 1105 46-47 0.21588339853478544 1105 48-49 0.2897436221500769 1105 50-51 0.25887714307521037 1105 52-53 0.2183702663366418 1105 54-55 0.2643184884172314 1105 56-57 0.20895772355763853 1105 58-59 0.22259090711401797 1105 60-61 0.24858120685541962 1105 62-63 0.186396602460988 1105 64-65 0.2746175964441875 1105 66-67 0.3057654509651826 1105 68-69 0.3371125231487113 1105 70-71 0.2464310820584359 1105 72-73 0.04897861801995873 1105 74-75 0.19914450984345677 1105 76-77 0.28297044807093386 1105 78-79 0.1240719450322274 1105 80-81 0.20298812650928255 1105 82-83 0.15196942554464243 1105 84-85 0.055861043635584195 1105 86-87 0.08770731229912698 1105 88-89 0.10058166841490745 1105 90-91 0.09468536154722074 1105 92-93 0.13359923976804566 1105 94-95 0.09194142844176412 1105 96-97 0.1728852496127189 1105 98-99 0.11920156937505055 1105 100-101 0.04077318214072889 1105 102-103 0.15851064125586234 1105 104-105 0.24226621843441265 1105 106-107 0.2623342656231955 1105 108-109 0.16272335283336403 1105 110-111 0.2148595145218124 1105 112-113 0.2897591387010152 1105 114-115 0.2427973350569239 1105 116-117 0.34590240751919055 1105 118-119 0.38810591638725356 1105 120-121 0.28165227974727003 1105 122-123 0.2164314180106146 1105 124-125 0.3819054110002895 1106 1 0.11222706337274246 1106 2 0.24842288631635157 1106 3 0.20043775329830282 1106 4 0.2339663502643674 1106 5 0.01879051422162803 1106 6 0.11837472042203245 1106 7 0.1938078121211646 1106 8 0.04360837877147361 1106 9 0.3196649007256198 1106 10-11 0.2671725808998673 1106 12-13 0.18336927205179165 1106 14-15 0.3754968991869134 1106 16-17 0.3393810592078168 1106 18-19 0.30360035605467317 1106 20-21 0.39509879912586854 1106 22-23 0.3596348206597355 1106 24-25 0.38353398542648165 1106 26-27 0.33093839094551214 1106 28-29 0.36595410587545274 1106 30-31 0.3176418560900274 1106 32-33 0.3731132782514166 1106 34-35 0.4632299575933203 1106 36-37 0.4264434747796173 1106 38-39 0.5037081573738931 1106 40-41 0.48490801646880044 1106 42-43 0.5445815172069004 1106 44-45 0.48785934101705664 1106 46-47 0.4407726405830772 1106 48-49 0.45535475097681655 1106 50-51 0.3904040968197222 1106 52-53 0.3353254543680677 1106 54-55 0.3891915076860073 1106 56-57 0.5996406968086916 1106 58-59 0.5877600036416766 1106 60-61 0.44950878848301556 1106 62-63 0.4469263684743012 1106 64-65 0.4469445523414848 1106 66-67 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114-115 -1.9413694161992296 2216 116-117 -2.089526298044138 2216 118-119 -2.11015903238777 2216 120-121 -2.0818572655721503 2216 122-123 -2.1197194400893125 2216 124-125 -2.4005958940946535 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 28441.0 3 5363.0 4 3141.0 5 2558.0 6 3120.0 7 3521.0 8 3649.0 9 4253.0 10 4825.0 11 5223.0 12 5475.0 13 5730.0 14 5911.0 15 5310.0 16 4846.0 17 4520.0 18 4331.0 19 4274.0 20 4389.0 21 4771.0 22 5759.0 23 7244.0 24 9374.0 25 11715.0 26 14298.0 27 16334.0 28 19653.0 29 24506.0 30 29192.0 31 34059.0 32 44043.0 33 58422.0 34 80539.0 35 119691.0 36 222666.0 37 437012.0 38 229000.0 39 1666.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.08044754146362 18.80012473224349 12.823099650600255 23.296328075692635 2 15.353999343785219 20.31881441145482 40.651215050279234 23.67597119448073 3 18.9035689540397 26.078973467197414 29.14206529270314 25.87539228605975 4 12.053423141741007 16.00805884642477 37.76049113859735 34.178026873236874 5 12.90085042412517 39.88019746088722 34.03021148857168 13.188740626415928 6 31.778437545880166 36.66545678972067 17.840451216130244 13.715654448268925 7 28.626685751803503 33.26217187226008 21.027294192490732 17.08384818344568 8 26.501131736893264 35.961500553973316 19.860996880076673 17.67637082905675 9 27.394511390587752 15.967080035592826 19.735596156029263 36.90281241779016 10-11 24.985603259136578 27.397218828421444 27.54961691411384 20.067560998328144 12-13 25.110814653440304 24.788960895465355 27.98954782725765 22.110676623836692 14-15 22.316013166535463 26.523111457360354 25.326002275999627 25.834873100104556 16-17 22.19190848733972 29.7986808483737 25.953659072107417 22.05575159217917 18-19 22.17673746358207 28.744456368000286 27.46578813816565 21.61301803025199 20-21 23.43587052141955 27.877230027478916 27.138181823126068 21.548717627975467 22-23 22.927723589507192 27.961453578261597 26.993169694274812 22.117653137956403 24-25 22.385953127915993 28.106865604872745 27.379271733059664 22.1279095341516 26-27 22.183928013535706 28.325581759023212 27.136549978871706 22.35394024856938 28-29 22.089064725216083 28.33545764463161 27.401542419947454 22.17393521020485 30-31 22.28139332596631 28.245555057395823 27.20131178790587 22.271739828732002 32-33 21.87784997962206 28.487440289580874 27.25990318524685 22.374806545550214 34-35 22.451114090236686 28.384521569352593 27.09476565219283 22.06959868821789 36-37 22.587122076905516 28.03575117141211 26.862946142221933 22.514180609460443 38-39 22.125206322467584 28.238430658669618 27.204542830166893 22.431820188695905 40-41 22.312839872792654 28.167244243813176 27.41054120178612 22.109374681608053 42-43 22.295279304077518 28.174147733737847 26.97837486978287 22.552198092401774 44-45 22.216749672043274 28.364163416537984 27.10002697023695 22.319059941181784 46-47 22.230012453046307 28.31631145252251 26.988088420025697 22.46558767440549 48-49 22.210229455932698 28.304117527750684 27.089994329765737 22.395658686550878 50-51 22.157411823210275 28.414842260728022 26.92148566630389 22.50626024975781 52-53 22.39917472237404 28.2274092308403 26.989764532967115 22.383651513818542 54-55 22.222414687304813 28.189285106565087 27.13170079921692 22.45659940691319 56-57 22.221976965154326 28.47876020967442 26.843853955981412 22.455408869189842 58-59 22.157120008803574 28.20278379042061 27.128659537085564 22.511436663690255 60-61 22.068639468050748 28.357143415113946 27.143832864467804 22.430384252367507 62-63 22.034793585055894 28.36315116533441 27.135925562082097 22.4661296875276 64-65 21.87408603352221 28.250018088256425 27.16163156412635 22.714264314095015 66-67 21.961588960775355 28.54877475001571 26.855760721680475 22.633875567528456 68-69 21.918371826045714 28.255051775668104 27.176628430462895 22.649947967823287 70-71 21.972719551554274 27.954204178698827 27.276371666383557 22.796704603363345 72-73 21.87902629561776 28.267925000135907 27.02483595818361 22.82821274606272 74-75 21.64455278200087 28.403774450267115 26.977874790850947 22.973797976881066 76-77 21.7995351869461 28.173847284349606 27.215276525038313 22.811341003665984 78-79 21.88756070760932 28.247937793005313 27.150664955154348 22.713836544231018 80-81 21.776660248916304 28.46059946340948 27.018661078569366 22.744079209104846 82-83 21.934527095780158 28.257175909803607 27.108928416721735 22.6993685776945 84-85 21.839899736465085 28.168372888484676 27.054880967628726 22.936846407421513 86-87 21.742184827475626 27.941430749909387 27.2311751523323 23.085209270282693 88-89 21.7408851729338 28.038142630936512 27.277335138842695 22.943637057286985 90-91 21.79765144178757 28.114492467609754 27.315109039717473 22.7727470508852 92-93 21.895370777662478 27.830360918677382 27.258318996336634 23.015949307323503 94-95 21.664479306348642 27.675882110789118 27.468898045912827 23.190740536949406 96-97 21.765805247172594 27.56645398628825 27.520130033102607 23.147610733436554 98-99 21.504692978033802 27.640175707867847 27.402327087006224 23.452804227092134 100-101 21.430683498237237 27.629746185483366 27.61239860065079 23.327171715628612 102-103 21.55444969839745 27.31354784397457 27.75602271608626 23.37597974154172 104-105 21.27405841335407 27.583450194667048 27.614782950228445 23.527708441750438 106-107 21.43361488424639 27.50335559534066 27.55607425557382 23.50695526483913 108-109 21.48092569738801 27.456403170308153 27.33869639834932 23.723974733954517 110-111 21.190217219668387 27.903832067772182 27.4140206811516 23.491930031407833 112-113 21.270115522158868 27.882340516496352 27.574883617002566 23.27266034434222 114-115 21.04406761244406 28.084427270442987 27.38653727309152 23.484967844021433 116-117 20.99556465208001 28.05996734350739 27.525538986616542 23.418929017796057 118-119 20.92064734311332 28.006448430895404 27.35912569820637 23.71377852778491 120-121 20.93124441268297 28.244414041590016 27.391949100730667 23.432392444996346 122-123 21.02667810694807 28.127948704802368 27.35043286434744 23.49494032390212 124-125 21.10572438455319 28.253216670351012 27.14353288897084 23.497526056124958 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 6580.0 1 3654.0 2 876.0 3 1050.0 4 990.0 5 834.0 6 704.0 7 611.0 8 584.0 9 588.5 10 542.5 11 509.0 12 553.0 13 588.0 14 628.0 15 693.5 16 757.5 17 830.0 18 1020.5 19 1277.5 20 1603.5 21 2090.0 22 2775.5 23 3807.5 24 4990.0 25 6305.5 26 8370.0 27 10883.5 28 13151.5 29 16017.0 30 19628.0 31 22793.0 32 26945.0 33 31242.0 34 34032.5 35 36259.5 36 40605.0 37 44382.5 38 44753.5 39 45874.5 40 46992.5 41 47486.5 42 47135.0 43 47575.0 44 50656.5 45 52910.0 46 52206.5 47 51155.0 48 51007.5 49 49942.5 50 48863.0 51 47468.0 52 46281.5 53 46212.5 54 45903.5 55 43257.5 56 39337.5 57 36501.0 58 34475.0 59 31273.0 60 27088.0 61 23337.5 62 19611.0 63 14884.5 64 10605.0 65 8039.5 66 5815.0 67 4628.0 68 3356.0 69 2043.5 70 1463.5 71 1041.0 72 833.0 73 694.0 74 528.5 75 388.5 76 250.5 77 136.5 78 94.5 79 59.5 80 39.0 81 28.0 82 18.0 83 15.5 84 16.0 85 12.5 86 8.0 87 10.0 88 8.0 89 5.5 90 6.5 91 4.5 92 4.5 93 6.0 94 4.0 95 3.5 96 2.0 97 0.5 98 1.0 99 2.0 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4646259460219742 2 0.4561056623371003 3 0.45198076309283597 4 0.4498845028211606 5 0.4554294493462373 6 0.5139218730558877 7 0.5086474117271562 8 0.5162209972248218 9 0.523456476227056 10-11 0.5413084991858396 12-13 0.5494230550761957 14-15 0.5289338014530465 16-17 0.5225097780398479 18-19 0.5328220261505088 20-21 0.5327205942018793 22-23 0.5427285464666518 24-25 0.5374878957874635 26-27 0.5441824043970074 28-29 0.5246736596106095 30-31 0.5311314936733513 32-33 0.532213434458732 34-35 0.5306919552292902 36-37 0.4798407383163919 38-39 0.44724727215679483 40-41 0.44508339058603325 42-43 0.45735665637019685 44-45 0.4619210940585222 46-47 0.46624885720004544 48-49 0.4803478980595392 50-51 0.4623944431521263 52-53 0.4621577686053242 54-55 0.45275840803232836 56-57 0.4358868939102963 58-59 0.45269078673324215 60-61 0.44991831347070377 62-63 0.46564026550826876 64-65 0.46486262056877625 66-67 0.4720980995710105 68-69 0.4827146435275598 70-71 0.47835306973649333 72-73 0.4901191757775097 74-75 0.4791983359750721 76-77 0.47669634790887894 78-79 0.4882934007021796 80-81 0.48176794534035156 82-83 0.4832556139202501 84-85 0.4802802767604529 86-87 0.4706104309911118 88-89 0.47554678582441184 90-91 0.46080534262359824 92-93 0.46094058522177084 94-95 0.4550913428508057 96-97 0.44518482253466274 98-99 0.4083312145326286 100-101 0.40549111997100395 102-103 0.39619319134663755 104-105 0.4006223864367903 106-107 0.3997094988991252 108-109 0.4122532498796341 110-111 0.43416255078359567 112-113 0.43429779338176827 114-115 0.4269608824309046 116-117 0.42077353356450803 118-119 0.4623944431521263 120-121 0.4557675558416688 122-123 0.42259930863983813 124-125 0.43125483492288463 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1478824.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.93165723034225 #Duplication Level Percentage of deduplicated Percentage of total 1 82.21922164220115 52.56411095774741 2 10.319258891114089 13.194546445957354 3 3.2832345613664318 6.297078797522755 4 1.4805891911887727 3.786260826801212 5 0.7374633199394341 2.3573626095159064 6 0.45393898297294616 1.7412642877749942 7 0.29512343112007366 1.320741102730775 8 0.19446490878226233 0.9945971113277895 9 0.1475953607177696 0.8492414409177451 >10 0.7650979894149853 9.106700057574823 >50 0.0704455623949015 3.085381450567622 >100 0.03290366881128898 3.6911582518062267 >500 4.416599839080737E-4 0.16023961044275659 >1k 0.0 0.0 >5k 2.2082999195403684E-4 0.8513170493126186 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 6030 0.40775643349039503 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 6.762129908630101E-5 0.0 16-17 0.0 0.0 0.0 1.3524259817260202E-4 0.0 18-19 0.0 0.0 0.0 1.3524259817260202E-4 0.0 20-21 0.0 0.0 0.0 2.02863897258903E-4 0.0 22-23 0.0 0.0 0.0 3.0429584588835453E-4 0.0 24-25 0.0 0.0 0.0 6.762129908630101E-4 0.0 26-27 0.0 0.0 0.0 0.0015891005285280737 0.0 28-29 0.0 0.0 0.0 0.003482496902944502 0.0 30-31 0.0 0.0 0.0 0.011056082400610213 0.0 32-33 0.0 0.0 0.0 0.023870318577464255 0.0 34-35 0.0 0.0 0.0 0.04277047167208539 0.0 36-37 0.0 0.0 0.0 0.07624301471980438 0.0 38-39 0.0 0.0 0.0 0.12935954515209383 0.0 40-41 0.0 0.0 0.0 0.18917058419392707 0.0 42-43 0.0 0.0 0.0 0.2500635640211411 0.0 44-45 0.0 0.0 0.0 0.32282408183800104 0.0 46-47 0.0 0.0 0.0 0.41299708416958336 0.0 48-49 0.0 0.0 0.0 0.515139056439441 0.0 50-51 0.0 0.0 0.0 0.6336453830881836 0.0 52-53 0.0 0.0 0.0 0.7442400177438289 0.0 54-55 0.0 0.0 0.0 0.8669050542863789 0.0 56-57 0.0 0.0 0.0 1.0101607764007077 0.0 58-59 0.0 0.0 0.0 1.1739057521381855 0.0 60-61 0.0 0.0 0.0 1.36047291631729 0.0 62-63 0.0 0.0 0.0 1.547411997641369 0.0 64-65 0.0 0.0 0.0 1.7360754220921488 0.0 66-67 0.0 0.0 0.0 1.95780566179613 0.0 68-69 0.0 0.0 0.0 2.211656018566104 0.0 70-71 0.0 0.0 0.0 2.505301509848366 0.0 72-73 0.0 0.0 0.0 2.811795047957025 0.0 74-75 0.0 0.0 0.0 3.1009437228500483 0.0 76-77 0.0 0.0 0.0 3.417377591924394 0.0 78-79 0.0 0.0 0.0 3.7934534467928573 0.0 80-81 0.0 0.0 0.0 4.216559915175843 0.0 82-83 0.0 0.0 0.0 4.658431294055276 0.0 84-85 0.0 0.0 0.0 5.088468945594608 0.0 86-87 0.0 0.0 0.0 5.532233720848458 0.0 88-89 0.0 0.0 0.0 6.012412565660281 0.0 90-91 0.0 0.0 0.0 6.567752484406529 0.0 92-93 0.0 0.0 0.0 7.165660010927602 0.0 94-95 0.0 0.0 0.0 7.757413999231822 0.0 96-97 0.0 0.0 0.0 8.332161230815839 0.0 98-99 0.0 0.0 0.0 8.932841230599449 0.0 100-101 0.0 0.0 0.0 9.622206564134746 0.0 102-103 0.0 0.0 0.0 10.359583020021315 0.0 104-105 0.0 0.0 0.0 11.093443168355396 0.0 106-107 0.0 0.0 0.0 11.792715022206835 0.0 108-109 0.0 0.0 0.0 12.508655526283047 0.0 110-111 0.0 0.0 0.0 13.304862512374697 0.0 112-113 0.0 0.0 0.0 14.147525330938638 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2085 0.0 61.935497 1 GTATCAA 3670 0.0 48.48318 1 ATCAACG 4335 0.0 39.546448 3 TCAACGC 4375 0.0 39.32763 4 GTACATG 10010 0.0 38.82085 1 CAACGCA 4455 0.0 38.4917 5 TACATGG 10115 0.0 37.717003 2 AACGCAG 4655 0.0 36.837917 6 TATCAAC 4890 0.0 36.02698 2 ACATGGG 10225 0.0 35.860924 3 CATGGGA 6450 0.0 31.75241 4 CATGGGG 3630 0.0 30.66994 4 GTGGTAT 1080 0.0 30.30574 1 ACGCAGA 5590 0.0 30.25127 7 CGCAGAG 5710 0.0 29.406956 8 ATGGGAG 2750 0.0 28.796602 5 TGGTATC 1195 0.0 27.891071 2 GAGTACA 7085 0.0 27.213923 1 AGTACAT 6945 0.0 27.080698 2 ATACCGT 180 7.247945E-7 26.463041 6 >>END_MODULE