FastQCFastQC Report
Thu 26 May 2016
SRR1364124_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364124_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1478824
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83620.565449302959649No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80210.5423904399712204No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49540.33499591567353515No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21690.14667059771818688No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA40900.084.100591
GTATCAA75600.053.6997031
GTGGTAT13000.049.5252881
TGGTATC15350.040.69682
ATCAACG101200.040.1517833
TATCAAC102500.039.7019272
TCAACGC103300.039.277944
CAACGCA104000.039.0135735
AACGCAG107400.038.1121526
GTACATG85450.036.7658961
TACATGG88450.034.842662
ACATGGG87500.033.859923
ACGCAGA124300.032.8390857
CGCAGAG124900.032.681338
CATGGGA55400.029.6390294
GCAGAGT137400.029.2750769
GAGTACT94600.028.55587412-13
CATGGGG32700.027.6541614
ATGGGAG23050.027.6170655
CAGAGTA132450.027.538910-11
Produced by FastQC (version 0.11.5)