##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364124_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1478824 Sequences flagged as poor quality 0 Sequence length 125 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.15610715000568 33.0 31.0 34.0 30.0 34.0 2 32.168734751397054 33.0 31.0 34.0 30.0 34.0 3 32.29576271415665 34.0 31.0 34.0 30.0 34.0 4 35.93299337852239 37.0 35.0 37.0 35.0 37.0 5 35.869833732749804 37.0 35.0 37.0 35.0 37.0 6 35.915912238373195 37.0 35.0 37.0 35.0 37.0 7 35.843096271091085 37.0 35.0 37.0 35.0 37.0 8 35.82611588667752 37.0 35.0 37.0 33.0 37.0 9 37.478950165807426 39.0 37.0 39.0 34.0 39.0 10-11 37.4087254467063 39.0 37.0 39.0 34.0 39.0 12-13 37.353628626530266 39.0 37.0 39.0 34.0 39.0 14-15 38.64407292551379 40.0 38.0 41.0 34.0 41.0 16-17 38.641601705138676 40.0 38.0 41.0 34.0 41.0 18-19 38.81687137887943 40.0 38.0 41.0 34.0 41.0 20-21 38.87989476773436 40.0 38.0 41.0 34.0 41.0 22-23 38.83728083936966 40.0 38.0 41.0 34.0 41.0 24-25 38.80979785288851 40.0 38.0 41.0 34.0 41.0 26-27 38.687667700821734 40.0 38.0 41.0 34.0 41.0 28-29 38.607432662710366 40.0 38.0 41.0 34.0 41.0 30-31 38.4313129892401 40.0 38.0 41.0 34.0 41.0 32-33 38.23976923555474 40.0 37.0 41.0 34.0 41.0 34-35 38.09949358409114 40.0 37.0 41.0 33.0 41.0 36-37 37.96298511519964 40.0 37.0 41.0 33.0 41.0 38-39 37.83773863556448 40.0 37.0 41.0 33.0 41.0 40-41 37.67954841144044 40.0 37.0 41.0 33.0 41.0 42-43 37.503716804704275 40.0 37.0 41.0 33.0 41.0 44-45 37.271652678073934 40.0 36.5 41.0 32.0 41.0 46-47 36.89492968737321 40.0 36.0 41.0 31.0 41.0 48-49 36.52934121978004 40.0 36.0 41.0 30.0 41.0 50-51 36.3195532396012 40.0 36.0 41.0 30.0 41.0 52-53 36.16488777569204 40.0 35.0 41.0 29.5 41.0 54-55 35.99187191984983 39.0 35.0 41.0 29.0 41.0 56-57 35.80078968153073 39.0 35.0 41.0 28.0 41.0 58-59 35.59690977425306 39.0 35.0 41.0 28.0 41.0 60-61 35.371575995520764 38.5 35.0 41.0 28.0 41.0 62-63 35.12777281136903 38.0 35.0 40.5 28.0 41.0 64-65 34.916364286757585 37.5 35.0 40.0 27.5 41.0 66-67 34.710162602175785 37.0 34.0 40.0 28.0 41.0 68-69 34.40250192044489 37.0 34.0 39.5 27.0 41.0 70-71 34.094259695541865 36.0 34.0 39.0 26.5 41.0 72-73 33.76826248424423 36.0 34.0 39.0 26.0 41.0 74-75 33.49800483357046 35.0 34.0 38.5 26.5 40.0 76-77 33.29643554608256 35.0 34.0 37.0 27.0 39.5 78-79 33.00717360551357 35.0 34.0 37.0 26.0 39.0 80-81 32.73336989391571 35.0 34.0 36.5 26.0 39.0 82-83 32.470517451704865 35.0 34.0 36.0 26.0 37.5 84-85 32.24874494868896 35.0 33.5 36.0 26.0 37.0 86-87 32.05831018430861 35.0 33.0 35.0 26.0 37.0 88-89 31.90166443065571 35.0 33.0 35.0 25.5 36.0 90-91 31.76387960974396 35.0 33.0 35.0 25.0 36.0 92-93 31.655945196994367 35.0 33.0 35.0 25.0 36.0 94-95 31.55717718944242 35.0 33.0 35.0 25.0 36.0 96-97 31.487935345923518 35.0 33.0 35.0 25.0 35.5 98-99 31.428245687113545 35.0 33.0 35.0 25.0 35.0 100-101 31.360870191449422 35.0 33.0 35.0 24.0 35.0 102-103 30.192166207743448 34.0 30.5 35.0 20.5 35.0 104-105 31.10203884978875 35.0 32.5 35.0 23.5 35.0 106-107 31.24447094448021 35.0 33.0 35.0 24.0 35.0 108-109 31.23431929695488 35.0 33.0 35.0 24.0 35.0 110-111 31.187100696228896 35.0 33.0 35.0 23.5 35.0 112-113 31.12552474128091 35.0 33.0 35.0 23.0 35.0 114-115 31.051500381384127 35.0 33.0 35.0 21.5 35.0 116-117 30.98789747799603 35.0 33.0 35.0 20.0 35.0 118-119 30.901784120355092 35.0 33.0 35.0 19.0 35.0 120-121 30.786580417953726 35.0 33.0 35.0 17.5 35.0 122-123 30.647833345955974 35.0 33.0 35.0 6.0 35.0 124-125 29.83104277452895 34.5 31.5 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.17472644876739452 1101 2 0.17731198158011097 1101 3 0.1939256136379086 1101 4 0.15719610310349452 1101 5 0.1041655979542071 1101 6 0.19009766348171553 1101 7 0.23590905852467614 1101 8 0.2237578222258989 1101 9 0.2661508432002577 1101 10-11 0.28306483053130904 1101 12-13 0.2023296692121761 1101 14-15 0.2995378888379321 1101 16-17 0.29610799215944184 1101 18-19 0.27882222418774205 1101 20-21 0.2857065604728106 1101 22-23 0.33835518788378494 1101 24-25 0.32025294347565847 1101 26-27 0.3438107707015874 1101 28-29 0.34777438135117933 1101 30-31 0.3858623673171877 1101 32-33 0.39472208178923296 1101 34-35 0.402214466257135 1101 36-37 0.3067872069412445 1101 38-39 0.41925815179335046 1101 40-41 0.37882487204548454 1101 42-43 0.472323499179538 1101 44-45 0.539637769579052 1101 46-47 0.5044845096729915 1101 48-49 0.5920999633206634 1101 50-51 0.4695612101250646 1101 52-53 0.5576653186761931 1101 54-55 0.5083230232134284 1101 56-57 0.49085557295450144 1101 58-59 0.48848650384974945 1101 60-61 0.6103496023370028 1101 62-63 0.5944797503759318 1101 64-65 0.49146642585824907 1101 66-67 0.41599716492780914 1101 68-69 0.5025623714028313 1101 70-71 0.5074893488278391 1101 72-73 0.4420062082339129 1101 74-75 0.35626894822063093 1101 76-77 0.378896906463396 1101 78-79 0.3941487186681414 1101 80-81 0.3329749405110576 1101 82-83 0.2794961728060201 1101 84-85 0.3309532078531703 1101 86-87 0.423893941891599 1101 88-89 0.35421078551500074 1101 90-91 0.3290494099157968 1101 92-93 0.3800722489737858 1101 94-95 0.32833347343785135 1101 96-97 0.3545505676334173 1101 98-99 0.3000911824848842 1101 100-101 0.30806605844723833 1101 102-103 0.4279287762220392 1101 104-105 0.3328446352237684 1101 106-107 0.38551262452082824 1101 108-109 0.2578787020188642 1101 110-111 0.2812821626899549 1101 112-113 0.2366664590985117 1101 114-115 0.1790624536887755 1101 116-117 0.2788914405864489 1101 118-119 0.2752658704631372 1101 120-121 0.4082342517123543 1101 122-123 0.3463190899769799 1101 124-125 0.40083085763013315 1102 1 0.36300206762824416 1102 2 0.3215504257837196 1102 3 0.24648319909145755 1102 4 0.18557166863651275 1102 5 0.2106982569485467 1102 6 0.2573274978214002 1102 7 0.18379680344563099 1102 8 0.20504814064464227 1102 9 0.36603171571234583 1102 10-11 0.28333974838924547 1102 12-13 0.36439498290022243 1102 14-15 0.42097793373652337 1102 16-17 0.39258566758219615 1102 18-19 0.3870453538431775 1102 20-21 0.44832375267662883 1102 22-23 0.4606697661638748 1102 24-25 0.36258932595660553 1102 26-27 0.506860713933257 1102 28-29 0.46454867131694755 1102 30-31 0.49912443918933036 1102 32-33 0.4688007790419917 1102 34-35 0.42369181913254295 1102 36-37 0.4526305392424277 1102 38-39 0.415862158261497 1102 40-41 0.5096439487859499 1102 42-43 0.6027130503656508 1102 44-45 0.5614864819700358 1102 46-47 0.44434329844317944 1102 48-49 0.3173842350517546 1102 50-51 0.3399009384971876 1102 52-53 0.3652889403238433 1102 54-55 0.25920605034180966 1102 56-57 0.3722891023966213 1102 58-59 0.4110930949973337 1102 60-61 0.32451437672587957 1102 62-63 0.44050521441405266 1102 64-65 0.37852485853616713 1102 66-67 0.4847374885073137 1102 68-69 0.400257663071649 1102 70-71 0.31840822171837857 1102 72-73 0.36809340783481304 1102 74-75 0.3673310321345511 1102 76-77 0.35399076774222493 1102 78-79 0.3578771940083172 1102 80-81 0.27693954201455284 1102 82-83 0.21130192188998365 1102 84-85 0.1769160977054014 1102 86-87 0.2519016959298881 1102 88-89 0.27138127114229604 1102 90-91 0.2974479408201347 1102 92-93 0.26739990117313184 1102 94-95 0.2244686035749588 1102 96-97 0.31751492830156636 1102 98-99 0.2356683702160467 1102 100-101 0.23411745529229577 1102 102-103 0.2953216044892315 1102 104-105 0.26541761693748 1102 106-107 0.17392661619480876 1102 108-109 0.20090114271315684 1102 110-111 0.20909606577092177 1102 112-113 0.22548600030715704 1102 114-115 0.23752695627775822 1102 116-117 0.26174998422065343 1102 118-119 0.203307276941608 1102 120-121 0.21096515260860826 1102 122-123 0.3101530383005979 1102 124-125 0.38726397851228 1103 1 0.21447947350729635 1103 2 0.22280494715516141 1103 3 0.11739995491192445 1103 4 0.13342943011188169 1103 5 0.17222711730821771 1103 6 0.20195725481360682 1103 7 0.11566610397036214 1103 8 0.10241364576462075 1103 9 0.2464216999193809 1103 10-11 0.18832631163750335 1103 12-13 0.1717218334784576 1103 14-15 0.1758524179180938 1103 16-17 0.29545100713082917 1103 18-19 0.3033741912775838 1103 20-21 0.34990304861631216 1103 22-23 0.31189849661372193 1103 24-25 0.32772031836579885 1103 26-27 0.38381604787792156 1103 28-29 0.3456789504957172 1103 30-31 0.362058745572746 1103 32-33 0.4020764808097823 1103 34-35 0.3811527188217809 1103 36-37 0.3742270928111182 1103 38-39 0.4023661829188896 1103 40-41 0.35006596468079465 1103 42-43 0.3779015469778031 1103 44-45 0.3290797113754991 1103 46-47 0.42533729966585554 1103 48-49 0.36053467572627085 1103 50-51 0.3768217571838193 1103 52-53 0.464757519976402 1103 54-55 0.3949022778623146 1103 56-57 0.25866194360096983 1103 58-59 0.3164649300373199 1103 60-61 0.2774214275180782 1103 62-63 0.23004142359435065 1103 64-65 0.24762637924532527 1103 66-67 0.22613764898415667 1103 68-69 0.2013644438551765 1103 70-71 0.21076264629426333 1103 72-73 0.13063225342042273 1103 74-75 0.11375469100663338 1103 76-77 0.09705874114892055 1103 78-79 0.05192681446784775 1103 80-81 0.014017653482277126 1103 82-83 0.04327502291398844 1103 84-85 -0.02387514229664589 1103 86-87 -0.09376447402273413 1103 88-89 -0.0791440994828001 1103 90-91 -0.05221454835405126 1103 92-93 -0.08026204034197448 1103 94-95 0.023247514879656705 1103 96-97 -0.06360070418098829 1103 98-99 0.0015523938545385363 1103 100-101 0.008012903353840528 1103 102-103 0.14721994368124314 1103 104-105 0.13565553022904098 1103 106-107 0.031270023907882916 1103 108-109 -0.021482193167425834 1103 110-111 -0.06726798741568984 1103 112-113 -0.0717238040639252 1103 114-115 -0.046690273331496 1103 116-117 -0.059990807465119644 1103 118-119 -0.12075533450064313 1103 120-121 -0.13436318811175596 1103 122-123 -0.0679288176268571 1103 124-125 0.021776370890904673 1104 1 0.06134052466234863 1104 2 0.0383964219775379 1104 3 0.03557337622510914 1104 4 0.032665798339344576 1104 5 0.07999265015597956 1104 6 0.060593857066017165 1104 7 0.1371107399117406 1104 8 0.15818274758360218 1104 9 0.20636622017728 1104 10-11 0.11534000457428562 1104 12-13 0.09373274457494318 1104 14-15 0.10410540409225177 1104 16-17 0.15818093181876947 1104 18-19 0.17799927713404884 1104 20-21 0.1297613167228917 1104 22-23 0.16793901663964306 1104 24-25 0.21653732039373352 1104 26-27 0.16812828090733944 1104 28-29 0.19982443621227475 1104 30-31 0.1855118641637148 1104 32-33 0.21398541525923065 1104 34-35 0.19627270040907518 1104 36-37 0.2819536617720715 1104 38-39 0.2866222958694564 1104 40-41 0.24833246437664513 1104 42-43 0.3357426214386976 1104 44-45 0.3787405443327927 1104 46-47 0.3057755557993431 1104 48-49 0.2668059888213321 1104 50-51 0.1559463564447796 1104 52-53 0.2572017276170655 1104 54-55 0.22257799442027704 1104 56-57 0.18353745549280376 1104 58-59 0.2983835722334547 1104 60-61 0.25009445284943865 1104 62-63 0.24266723315216154 1104 64-65 0.2146442041663832 1104 66-67 0.17770795403212247 1104 68-69 0.1517691548005331 1104 70-71 0.01288560423924423 1104 72-73 0.13512971291459763 1104 74-75 0.07732224864750492 1104 76-77 0.12067115860740074 1104 78-79 0.22795478147721582 1104 80-81 0.2233996137463663 1104 82-83 0.17348901016403317 1104 84-85 0.21050653102408745 1104 86-87 0.17397844164793952 1104 88-89 0.14654047184497188 1104 90-91 0.21993732743995054 1104 92-93 0.20677645658229693 1104 94-95 0.13621560255021592 1104 96-97 0.18778190970985165 1104 98-99 0.15635531209863984 1104 100-101 0.1344733695069067 1104 102-103 0.16969395114418617 1104 104-105 0.2036550638705812 1104 106-107 0.257418235284252 1104 108-109 0.2553459531362279 1104 110-111 0.2333604487309593 1104 112-113 0.3054239669299079 1104 114-115 0.31678328770800235 1104 116-117 0.2321715915987852 1104 118-119 0.3827136596088607 1104 120-121 0.2837875797788634 1104 122-123 0.2858877403939921 1104 124-125 0.35934438401660174 1105 1 0.15046009177357433 1105 2 0.17876167285564293 1105 3 0.07732951422569556 1105 4 0.043558138078097386 1105 5 0.020444026601438736 1105 6 0.10027493995615089 1105 7 0.09230971153284173 1105 8 0.02677832394603996 1105 9 0.10997531654449233 1105 10-11 0.028887338770424265 1105 12-13 0.06841903404114902 1105 14-15 0.08825550265882498 1105 16-17 0.13469471880502226 1105 18-19 0.07918184294173614 1105 20-21 0.16264714285141224 1105 22-23 0.13775146141507122 1105 24-25 0.20028272202848285 1105 26-27 0.12076096701694183 1105 28-29 0.05922357344153539 1105 30-31 0.1911176984813565 1105 32-33 0.2117169952360669 1105 34-35 0.1734747732498647 1105 36-37 0.19266782608698918 1105 38-39 0.10244401322570695 1105 40-41 0.16580119238514612 1105 42-43 0.15440314160682078 1105 44-45 0.17869555858313646 1105 46-47 0.02579355398398775 1105 48-49 0.02988443981202238 1105 50-51 -0.014241668838138821 1105 52-53 -0.020685698069520697 1105 54-55 -0.0035874735004739478 1105 56-57 -0.016201118253036384 1105 58-59 -0.1122801870640373 1105 60-61 -0.07541784762585024 1105 62-63 -0.0930276577991549 1105 64-65 -0.13041446280640656 1105 66-67 -0.03391484084548324 1105 68-69 -0.046287212188396154 1105 70-71 -0.07814566299339987 1105 72-73 -0.06108206352332246 1105 74-75 -0.11087182795685635 1105 76-77 -0.11889825628843909 1105 78-79 -0.1759164404045066 1105 80-81 -0.09616120413185314 1105 82-83 -0.0843981125097315 1105 84-85 -0.0361900950641072 1105 86-87 -0.03278459086553909 1105 88-89 -0.05423485250014437 1105 90-91 0.0255496493397942 1105 92-93 0.019062247398171195 1105 94-95 -0.0398409652818259 1105 96-97 0.01309773744861431 1105 98-99 -0.008404024823839507 1105 100-101 0.04846347147654839 1105 102-103 0.14713639473281503 1105 104-105 0.10330809476603875 1105 106-107 0.044147613950634934 1105 108-109 -0.00864211903598644 1105 110-111 -0.025129203284066648 1105 112-113 0.023317281942620838 1105 114-115 0.058767967302049584 1105 116-117 0.012084152975873508 1105 118-119 0.05696798098930955 1105 120-121 0.05476009748714361 1105 122-123 0.026844992860198147 1105 124-125 0.12071576565988806 1106 1 0.12207541737955552 1106 2 0.18396411065503315 1106 3 0.12070334492177182 1106 4 0.07767172175351789 1106 5 0.010439915164781155 1106 6 0.0820721740491237 1106 7 0.16815927869793512 1106 8 0.10001590828190388 1106 9 0.07746997926925303 1106 10-11 0.16779729100356633 1106 12-13 0.13658430879718253 1106 14-15 0.1398528794123166 1106 16-17 0.2258269515650042 1106 18-19 0.22597377868261503 1106 20-21 0.14235050433049423 1106 22-23 0.2110207609004604 1106 24-25 0.21279002372086353 1106 26-27 0.21230309405783743 1106 28-29 0.2821138000049572 1106 30-31 0.15693203492065777 1106 32-33 0.21982780835291038 1106 34-35 0.1910876373758157 1106 36-37 0.20277630919751743 1106 38-39 0.2624548232863688 1106 40-41 0.32097688374176414 1106 42-43 0.376491359313448 1106 44-45 0.21813757569739778 1106 46-47 0.39934746329903703 1106 48-49 0.3744850124915118 1106 50-51 0.3829966336055932 1106 52-53 0.36148473683743987 1106 54-55 0.3499313992239337 1106 56-57 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64-65 -0.8540684930121003 2216 66-67 -0.8730835497440594 2216 68-69 -0.7784232719617492 2216 70-71 -0.8232584449482871 2216 72-73 -0.8332878914973136 2216 74-75 -0.7703055798873066 2216 76-77 -0.5031472103998524 2216 78-79 -0.4196653075095895 2216 80-81 -0.44919509672254065 2216 82-83 -0.4259476227366008 2216 84-85 -0.440574035870803 2216 86-87 -0.4653000344890579 2216 88-89 -0.5664374902420413 2216 90-91 -0.4353465339825391 2216 92-93 -0.3720847679221926 2216 94-95 -0.48140950814331873 2216 96-97 -0.6308495222145254 2216 98-99 -0.5795335119940361 2216 100-101 -0.6297979970452872 2216 102-103 -0.8209200469983244 2216 104-105 -0.7125802579866232 2216 106-107 -0.8257811858695518 2216 108-109 -0.7445527127816796 2216 110-111 -0.8626935517154166 2216 112-113 -0.8250718321154267 2216 114-115 -0.8598242884095519 2216 116-117 -0.9319886314441916 2216 118-119 -1.0176247206297333 2216 120-121 -1.1901884600204156 2216 122-123 -1.242150392179063 2216 124-125 -1.664121115598224 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 9.0 3 6.0 4 25.0 5 90.0 6 306.0 7 700.0 8 1368.0 9 2379.0 10 4010.0 11 5959.0 12 7351.0 13 10995.0 14 16778.0 15 15781.0 16 6879.0 17 5192.0 18 4897.0 19 4427.0 20 4179.0 21 4155.0 22 4454.0 23 4839.0 24 5945.0 25 7767.0 26 10092.0 27 13025.0 28 16703.0 29 21383.0 30 27798.0 31 34950.0 32 44950.0 33 58973.0 34 78899.0 35 118224.0 36 222445.0 37 461967.0 38 248995.0 39 1929.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.225949698041944 19.186731727405977 13.879978108379776 22.707340466172298 2 15.8875667019886 20.831239826911254 39.76679409161207 23.514399379488076 3 18.772145975450762 26.07896544822102 30.459067475237077 24.689821101091137 4 12.283679464222923 16.737150600747622 37.325266563160994 33.65390337186846 5 12.25027454247429 39.25849188273926 34.429993021481934 14.061240553304518 6 31.052241510822114 37.71632053577708 16.800511757991483 14.430926195409324 7 27.072930923490556 34.316118753820604 21.08547061719312 17.52547970549572 8 26.46927558654715 35.512339534657265 19.41035579622727 18.60802908256831 9 27.031023996786637 14.947563028164968 20.055679415318803 37.9657335597296 10-11 24.932462907851345 27.047024750188836 27.263853472065847 20.756658869893975 12-13 25.744068400090214 24.585931738543994 28.373181651728547 21.29681820963724 14-15 22.550181766051942 25.65562230529123 27.076244367145787 24.71795156151104 16-17 21.175512351410145 28.959663082500292 27.661532614877142 22.20329195121242 18-19 21.02254899839332 26.568543653605836 31.268325372052384 21.140581975948454 20-21 22.168594775307948 25.67891784282646 31.46378473706134 20.68870264480425 22-23 21.63871427566769 25.763004928240278 31.48045338728611 21.117827408805915 24-25 21.203841701243693 25.92130638940131 31.767404369958836 21.10744753939617 26-27 21.06849767112246 26.15182739798651 31.566569111672514 21.213105819218512 28-29 20.995568100057884 26.061958691500813 31.753237707800253 21.18923550064105 30-31 21.211821014535875 25.963130162886184 31.622221440820542 21.2028273817574 32-33 20.969060550816053 26.153653173061837 31.558894094226225 21.318392181895884 34-35 21.411337657490005 26.09654698598346 31.32276051781686 21.16935483870968 36-37 21.64693026350668 25.767704608526778 31.043045014146376 21.54232011382017 38-39 21.283736266114158 26.00377056363705 31.23123508950355 21.481258080745242 40-41 21.397576723125944 25.97871687232558 31.3292183518796 21.29448805266888 42-43 21.540866255889814 25.97689109725025 30.74449697868036 21.73774566817958 44-45 21.507799440636614 26.23229674389921 30.55360205135973 21.70630176410445 46-47 21.841578172926596 26.3747410104245 29.872283652415703 21.9113971642332 48-49 22.021349396547528 26.645564313265137 29.35285064348428 21.980235646703054 50-51 21.99808766966184 26.88602565281602 28.843865125261694 22.272021552260448 52-53 22.145806397515862 26.920613947298666 28.80302186061357 22.130557794571903 54-55 21.992272237940416 26.894884042996324 28.842952237724027 22.269891481339226 56-57 22.04481398733047 27.112895111250563 28.580649218568265 22.261641682850698 58-59 21.909740442405585 26.751019729190222 28.913717927217842 22.42552190118635 60-61 21.922622299881528 26.936302088686688 28.985768421394297 22.15530719003749 62-63 21.881373307438885 26.818573406977436 29.19228386880386 22.107769416779817 64-65 21.704949338122724 26.652022147327877 29.253920682921024 22.389107831628372 66-67 21.855001000795227 27.065086852796544 28.83439814338961 22.245514003018616 68-69 21.832415486900402 26.756395622467583 29.061943814815017 22.349245075817002 70-71 21.974318783033002 26.618955332074673 28.975557605232265 22.431168279660056 72-73 21.906629862647616 27.006053458694208 28.541699350294557 22.54561732836362 74-75 21.641757234126576 27.175275759657673 28.402872823270382 22.78009418294537 76-77 21.752149004884963 27.073979053626395 28.491997695466125 22.681874246022517 78-79 21.832449297549942 27.098965123638784 28.321186293974133 22.74739928483714 80-81 21.733586958285773 27.400150389769166 28.12772175728822 22.738540894656836 82-83 21.94402444104234 27.199822291226 28.02067724083461 22.835476026897048 84-85 21.76411797482324 27.156578470460314 27.95390120798689 23.12540234672956 86-87 21.674553564183434 26.969707010435318 28.12035103568782 23.23538838969343 88-89 21.669008617658356 27.079287325604668 28.100707048303246 23.15099700843373 90-91 21.643785873099162 27.235289662596767 28.05753084883664 23.063393615467426 92-93 21.698878094268213 27.065646125330755 27.941443972672865 23.294031807728174 94-95 21.588721753921984 26.913132606826494 28.06101386146916 23.437131777782362 96-97 21.628439017561703 26.749255610730565 28.114955478657684 23.507349893050044 98-99 21.39573570283813 26.82957012151827 27.947544952292073 23.827149223351523 100-101 21.352233751338954 26.814517219739674 28.062075998398665 23.771173030522704 102-103 21.487467497996626 26.61799960101572 28.060361587957438 23.834171313030218 104-105 21.224256544387543 26.823171772948683 27.955520313258187 23.99705136940559 106-107 21.359156325093213 26.69492127238382 27.907436802497532 24.03848560002543 108-109 21.45085062166993 26.644132223858662 27.64543254800969 24.259584606461715 110-111 21.237906511969214 26.929530598511413 27.617451820490537 24.215111069028836 112-113 21.15702574366047 26.990684016461497 27.73567186039992 24.11661837947812 114-115 21.020478080023157 27.109554058701956 27.469191228965506 24.40077663230938 116-117 20.868562995048542 27.061453065652724 27.65491102696162 24.415072912337116 118-119 20.82159771267413 27.099468578858467 27.449804119824222 24.629129588643174 120-121 20.889717805685144 27.274928517198987 27.348820850827103 24.486532826288762 122-123 20.83941452804744 27.30369632937058 27.23782329524781 24.61906584733417 124-125 20.86950052667797 27.391380065365556 27.05778916987774 24.681330238078733 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 481.0 1 363.5 2 368.0 3 508.5 4 353.5 5 269.5 6 661.0 7 1584.0 8 2572.5 9 2940.0 10 2374.0 11 1680.5 12 1603.0 13 1744.0 14 1939.0 15 2187.5 16 2439.0 17 2679.0 18 2906.0 19 3149.5 20 3455.0 21 3863.0 22 4519.5 23 5380.5 24 6407.5 25 7664.5 26 9598.0 27 12091.0 28 14336.5 29 16874.0 30 20075.5 31 23267.5 32 27372.5 33 31275.0 34 33805.0 35 35963.5 36 39892.5 37 43162.0 38 43513.5 39 44328.0 40 45277.0 41 46095.0 42 46084.0 43 46563.0 44 49094.0 45 51502.5 46 51419.0 47 50645.5 48 50568.5 49 49424.0 50 48439.0 51 47086.5 52 45626.5 53 45441.5 54 45160.5 55 42079.5 56 38139.0 57 35683.0 58 33721.0 59 30508.0 60 26572.5 61 22747.0 62 18780.5 63 14427.5 64 10478.5 65 7849.5 66 5507.5 67 4377.0 68 3147.5 69 1832.0 70 1361.5 71 982.5 72 794.5 73 641.5 74 456.0 75 370.5 76 239.5 77 111.0 78 73.5 79 40.5 80 19.0 81 11.0 82 5.0 83 6.0 84 7.5 85 6.0 86 5.0 87 4.5 88 2.0 89 0.5 90 0.5 91 1.0 92 1.0 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.16634839575230048 2 0.003042958458883545 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 6.762129908630101E-5 10-11 4.733490936041071E-4 12-13 0.007607396147208863 14-15 0.0 16-17 3.3810649543150503E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 6.762129908630101E-5 94-95 8.452662385787627E-4 96-97 0.006424023413198596 98-99 0.0022991241689342343 100-101 0.0037867927488328566 102-103 0.004665869636954769 104-105 0.011597052793300623 106-107 0.01656721827614375 108-109 0.0234307801334033 110-111 0.01616149048162594 112-113 0.023227916236144397 114-115 0.026338495994114246 116-117 0.02505369131147452 118-119 0.016330543729341694 120-121 0.021740247656245774 122-123 0.015011928397158824 124-125 0.014640011252184167 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1478824.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.961239954477094 #Duplication Level Percentage of deduplicated Percentage of total 1 80.79344006900568 46.82887966587303 2 11.085461840602079 12.85054227498673 3 3.5264254944707223 6.131879827998092 4 1.4950505428295446 3.466199330280579 5 0.8436482038542449 2.4449447990379745 6 0.4900820087522258 1.7043456543995945 7 0.31261361086699596 1.2683630758748237 8 0.22066453538297903 1.0231992067820839 9 0.16425698171411257 0.8568484498196851 >10 0.9220374790997934 10.176695842039324 >50 0.09442986685200369 3.8200409504930186 >100 0.0474483847465493 4.861699538338533 >500 0.0032723023959676 1.1817590444816883 >1k 7.012076562787714E-4 1.165095469740057 >5k 4.6747177085251426E-4 2.219506869854809 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8362 0.565449302959649 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8021 0.5423904399712204 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4954 0.33499591567353515 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2169 0.14667059771818688 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 6.762129908630101E-5 0.0 16-17 0.0 0.0 0.0 1.3524259817260202E-4 0.0 18-19 0.0 0.0 0.0 1.3524259817260202E-4 0.0 20-21 0.0 0.0 0.0 2.02863897258903E-4 0.0 22-23 0.0 0.0 0.0 3.0429584588835453E-4 0.0 24-25 0.0 0.0 0.0 6.424023413198596E-4 0.0 26-27 0.0 0.0 0.0 0.0015214792294417725 0.0 28-29 0.0 0.0 0.0 0.003482496902944502 0.0 30-31 0.0 0.0 0.0 0.011123703699696515 0.0 32-33 0.0 0.0 0.0 0.024039371825180008 0.0 34-35 6.762129908630101E-5 0.0 0.0 0.0431085781675169 0.0 36-37 6.762129908630101E-5 0.0 0.0 0.07661493186477905 0.0 38-39 6.762129908630101E-5 0.0 0.0 0.13017100074112944 0.0 40-41 6.762129908630101E-5 0.0 0.0 0.1905230101756531 0.0 42-43 6.762129908630101E-5 0.0 0.0 0.25209220299373014 0.0 44-45 6.762129908630101E-5 0.0 0.0 0.32549512315190987 0.0 46-47 6.762129908630101E-5 0.0 0.0 0.41576955743212174 0.0 48-49 6.762129908630101E-5 0.0 0.0 0.5181820148983246 0.0 50-51 6.762129908630101E-5 0.0 0.0 0.6371278799911282 0.0 52-53 6.762129908630101E-5 0.0 0.0 0.7481620530908344 0.0 54-55 6.762129908630101E-5 0.0 0.0 0.8712666280774454 0.0 56-57 6.762129908630101E-5 0.0 0.0 1.015401427079896 0.0 58-59 6.762129908630101E-5 0.0 0.0 1.1797549945091506 0.0 60-61 6.762129908630101E-5 0.0 0.0 1.368350797660844 0.0 62-63 6.762129908630101E-5 0.0 0.0 1.5577580564015732 0.0 64-65 6.762129908630101E-5 0.0 0.0 1.7493630073626072 0.0 66-67 6.762129908630101E-5 0.0 0.0 1.97379809903004 0.0 68-69 6.762129908630101E-5 0.0 0.0 2.2312323846515882 0.0 70-71 6.762129908630101E-5 0.0 0.0 2.527075568154155 0.0 72-73 6.762129908630101E-5 0.0 0.0 2.8367811179694136 0.0 74-75 6.762129908630101E-5 0.0 0.0 3.12897275132132 0.0 76-77 6.762129908630101E-5 0.0 0.0 3.4499034367849046 0.0 78-79 6.762129908630101E-5 0.0 0.0 3.8289884394627083 0.0 80-81 6.762129908630101E-5 0.0 0.0 4.255475972800008 0.0 82-83 6.762129908630101E-5 0.0 0.0 4.700998901830102 0.0 84-85 6.762129908630101E-5 0.0 0.0 5.135296695211871 0.0 86-87 6.762129908630101E-5 0.0 0.0 5.584674038289885 0.0 88-89 6.762129908630101E-5 0.0 0.0 6.069248267542317 0.0 90-91 6.762129908630101E-5 0.0 0.0 6.62905119202826 0.0 92-93 6.762129908630101E-5 0.0 0.0 7.231861262733091 0.0 94-95 6.762129908630101E-5 0.0 0.0 7.828044446127463 0.0 96-97 6.762129908630101E-5 0.0 0.0 8.409046647876963 0.0 98-99 6.762129908630101E-5 0.0 0.0 9.016759262765548 0.0 100-101 6.762129908630101E-5 0.0 0.0 9.716741140257394 0.0 102-103 6.762129908630101E-5 0.0 0.0 10.463145039571984 0.0 104-105 6.762129908630101E-5 0.0 0.0 11.205119743796422 0.0 106-107 6.762129908630101E-5 0.0 0.0 11.913588094323597 0.0 108-109 6.762129908630101E-5 0.0 0.0 12.6382179353324 0.0 110-111 6.762129908630101E-5 0.0 0.0 13.441863264323544 0.0 112-113 6.762129908630101E-5 0.0 0.0 14.29534549074129 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4090 0.0 84.10059 1 GTATCAA 7560 0.0 53.699703 1 GTGGTAT 1300 0.0 49.525288 1 TGGTATC 1535 0.0 40.6968 2 ATCAACG 10120 0.0 40.151783 3 TATCAAC 10250 0.0 39.701927 2 TCAACGC 10330 0.0 39.27794 4 CAACGCA 10400 0.0 39.013573 5 AACGCAG 10740 0.0 38.112152 6 GTACATG 8545 0.0 36.765896 1 TACATGG 8845 0.0 34.84266 2 ACATGGG 8750 0.0 33.85992 3 ACGCAGA 12430 0.0 32.839085 7 CGCAGAG 12490 0.0 32.68133 8 CATGGGA 5540 0.0 29.639029 4 GCAGAGT 13740 0.0 29.275076 9 GAGTACT 9460 0.0 28.555874 12-13 CATGGGG 3270 0.0 27.654161 4 ATGGGAG 2305 0.0 27.617065 5 CAGAGTA 13245 0.0 27.5389 10-11 >>END_MODULE