FastQCFastQC Report
Thu 26 May 2016
SRR1364123_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364123_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2632278
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN107510.40842950478634854No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA24050.050.9773641
GTATCAA38350.041.590551
ATCAACG41500.036.430123
TCAACGC41650.036.301694
CAACGCA41800.036.0317655
AACGCAG44150.034.1138766
TATCAAC46750.033.353132
TACATGG84900.032.1752472
ACATGGG80800.031.4551283
GTACATG89200.030.8916661
ACGCAGA54050.027.7579637
CATGGGG56350.027.3598654
CGCAGAG55000.026.7372688
GTGTAGC17600.026.0377391
GAGTACA60750.023.4140661
AGAGTAC56150.020.72886810-11
TAGGTCG1500.004693431319.843585
CATGGGA34300.019.610674
GTACCGT3652.575689E-919.571756
AGTACAT66700.019.540482
Produced by FastQC (version 0.11.5)