##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364123_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2632278 Sequences flagged as poor quality 0 Sequence length 125 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.04313412185187 31.0 31.0 34.0 28.0 34.0 2 31.08455451893759 33.0 31.0 34.0 28.0 34.0 3 31.17624734165616 33.0 31.0 34.0 28.0 34.0 4 34.655060369763376 37.0 35.0 37.0 32.0 37.0 5 34.684911700056 37.0 35.0 37.0 32.0 37.0 6 34.7689305612857 37.0 35.0 37.0 32.0 37.0 7 34.65223126128775 37.0 35.0 37.0 32.0 37.0 8 34.63410133732076 37.0 35.0 37.0 32.0 37.0 9 36.179058214975775 39.0 37.0 39.0 32.0 39.0 10-11 36.09297498212575 39.0 37.0 39.0 31.0 39.0 12-13 36.002372089878044 39.0 37.0 39.0 31.0 39.0 14-15 37.27443548895671 40.0 38.0 41.0 31.0 41.0 16-17 37.27360635920674 40.0 38.0 41.0 31.0 41.0 18-19 37.26882134029916 40.0 38.0 41.0 31.0 41.0 20-21 37.28789398384213 40.0 38.0 41.0 31.0 41.0 22-23 37.24126023163207 40.0 37.5 41.0 31.0 41.0 24-25 37.240147697165725 40.0 37.0 41.0 31.0 41.0 26-27 37.20000831978993 40.0 37.0 41.0 31.0 41.0 28-29 37.159749274202795 40.0 37.0 41.0 31.0 41.0 30-31 37.103132723823244 40.0 37.0 41.0 31.0 41.0 32-33 37.006858888004984 40.0 37.0 41.0 30.0 41.0 34-35 36.97524672545985 40.0 37.0 41.0 30.0 41.0 36-37 36.905449006525906 40.0 37.0 41.0 30.0 41.0 38-39 36.81449869656625 40.0 37.0 41.0 30.0 41.0 40-41 36.73529030748272 40.0 37.0 41.0 30.0 41.0 42-43 36.61557479871047 40.0 36.0 41.0 30.0 41.0 44-45 36.50517308582148 40.0 36.0 41.0 30.0 41.0 46-47 36.39765423712845 40.0 36.0 41.0 30.0 41.0 48-49 36.277812221961355 40.0 36.0 41.0 29.0 41.0 50-51 36.144366020610285 40.0 35.0 41.0 29.0 41.0 52-53 35.995960533044 39.0 35.0 41.0 28.5 41.0 54-55 35.81547085832119 39.0 35.0 41.0 28.0 41.0 56-57 35.619782940859594 39.0 35.0 41.0 28.0 41.0 58-59 35.40237068425144 39.0 35.0 41.0 28.0 41.0 60-61 35.14885414838402 38.0 34.5 40.5 27.5 41.0 62-63 34.88309213540515 38.0 34.0 40.0 27.0 41.0 64-65 34.591880492865876 37.0 34.0 40.0 26.5 41.0 66-67 34.28640933822339 37.0 34.0 40.0 26.0 41.0 68-69 33.96248610519102 36.0 34.0 39.0 26.0 41.0 70-71 33.620862044206575 36.0 33.0 39.0 26.0 41.0 72-73 33.277010444945404 35.0 33.0 38.5 26.0 40.0 74-75 33.010685801423705 35.0 33.0 37.5 26.0 40.0 76-77 32.79262049829083 35.0 33.0 37.0 26.0 39.0 78-79 32.494328866479904 35.0 33.0 36.5 25.0 39.0 80-81 32.30420343140048 35.0 33.0 36.0 25.5 39.0 82-83 32.21655710377095 35.0 33.0 36.0 26.0 37.0 84-85 32.011336188654845 35.0 33.0 35.5 25.5 37.0 86-87 31.8305055545045 35.0 33.0 35.0 25.0 36.5 88-89 31.654219843040895 35.0 33.0 35.0 25.0 36.0 90-91 31.51593030827291 35.0 33.0 35.0 25.0 36.0 92-93 31.40070710616432 35.0 33.0 35.0 24.0 36.0 94-95 31.29729572636325 35.0 33.0 35.0 24.0 36.0 96-97 31.21559842843347 35.0 33.0 35.0 24.0 35.0 98-99 31.144082425944372 35.0 33.0 35.0 23.5 35.0 100-101 31.066853501035986 35.0 33.0 35.0 23.0 35.0 102-103 29.944890699234655 33.5 30.0 35.0 19.5 35.0 104-105 30.789151449808873 35.0 32.0 35.0 20.0 35.0 106-107 30.88896841443039 35.0 33.0 35.0 20.0 35.0 108-109 30.869505614528556 35.0 33.0 35.0 20.0 35.0 110-111 30.796574298003478 35.0 33.0 35.0 19.0 35.0 112-113 30.7176928120814 35.0 33.0 35.0 18.5 35.0 114-115 30.6142531297986 35.0 32.0 35.0 17.5 35.0 116-117 30.515094910188058 35.0 32.0 35.0 12.5 35.0 118-119 30.38162933398372 35.0 32.0 35.0 4.5 35.0 120-121 30.23958126763207 35.0 32.0 35.0 2.0 35.0 122-123 30.0803074371324 35.0 32.0 35.0 2.0 35.0 124-125 29.23779574193911 34.5 30.5 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.485658600044772 1101 2 0.4533300233600208 1101 3 0.443552092299651 1101 4 0.5172854600884733 1101 5 0.48727126853873415 1101 6 0.4362127380217018 1101 7 0.39648956918656353 1101 8 0.3608541341968987 1101 9 0.38962925735648923 1101 10-11 0.49146659229307943 1101 12-13 0.5539167430578686 1101 14-15 0.622283031452227 1101 16-17 0.5862018944908556 1101 18-19 0.6466694386839649 1101 20-21 0.6439132909575207 1101 22-23 0.674433383514554 1101 24-25 0.6866891684628555 1101 26-27 0.6285443405031543 1101 28-29 0.6226007427128195 1101 30-31 0.6541211875865685 1101 32-33 0.6477559214072102 1101 34-35 0.6714478289531627 1101 36-37 0.7089339353473747 1101 38-39 0.6538524778230865 1101 40-41 0.702848818514461 1101 42-43 0.616317414624028 1101 44-45 0.6609794605164225 1101 46-47 0.6833246794479919 1101 48-49 0.6533027120078074 1101 50-51 0.6472907369305929 1101 52-53 0.708051698990019 1101 54-55 0.7185666780113564 1101 56-57 0.7043586753705284 1101 58-59 0.7124714932428589 1101 60-61 0.6963496171884458 1101 62-63 0.6101198334250952 1101 64-65 0.7114034421387458 1101 66-67 0.7445956360027068 1101 68-69 0.7584168985836612 1101 70-71 0.7661370144780264 1101 72-73 0.723056003318213 1101 74-75 0.7972669932035785 1101 76-77 0.7391833273154234 1101 78-79 0.7520986713631928 1101 80-81 0.7009701090187903 1101 82-83 0.7141914582942661 1101 84-85 0.6998037552548624 1101 86-87 0.6607807785324553 1101 88-89 0.7635364412787027 1101 90-91 0.7192644956054863 1101 92-93 0.687991475230433 1101 94-95 0.7270242647826812 1101 96-97 0.67420070298909 1101 98-99 0.6788199308099649 1101 100-101 0.6601506846764131 1101 102-103 0.7558661608135608 1101 104-105 0.7196635383428003 1101 106-107 0.6797046431604521 1101 108-109 0.6933497660785832 1101 110-111 0.700056332421628 1101 112-113 0.591950254211806 1101 114-115 0.7214464733764849 1101 116-117 0.7007439659177059 1101 118-119 0.6580157678873562 1101 120-121 0.688774107046612 1101 122-123 0.698829782212318 1101 124-125 0.8049831121547619 1102 1 0.25694168505181736 1102 2 0.3911027866371697 1102 3 0.315774249690957 1102 4 0.324634869987257 1102 5 0.26912779799793185 1102 6 0.27602667193225017 1102 7 0.26201830999345077 1102 8 0.2980471043692887 1102 9 0.3035639663472338 1102 10-11 0.3133347831277291 1102 12-13 0.39310993802291705 1102 14-15 0.42765711162950737 1102 16-17 0.4592387717481188 1102 18-19 0.33102726766178137 1102 20-21 0.44585764515050386 1102 22-23 0.48076518690654524 1102 24-25 0.5271229200374776 1102 26-27 0.49537720633735205 1102 28-29 0.5330166685156783 1102 30-31 0.5646968021682781 1102 32-33 0.5026057822220551 1102 34-35 0.567760881197394 1102 36-37 0.5372305208122938 1102 38-39 0.5434047575748977 1102 40-41 0.5362483817451817 1102 42-43 0.5278647763709117 1102 44-45 0.5381548362610502 1102 46-47 0.4636005970903412 1102 48-49 0.4545998148234318 1102 50-51 0.4767895946440035 1102 52-53 0.5128005361793271 1102 54-55 0.446942104692603 1102 56-57 0.37197620682358234 1102 58-59 0.40517295855119073 1102 60-61 0.41650741836404137 1102 62-63 0.4172694276211004 1102 64-65 0.4433400309530384 1102 66-67 0.3506288827970039 1102 68-69 0.40331337828547476 1102 70-71 0.4483507145059278 1102 72-73 0.3807478535194946 1102 74-75 0.3763774284353474 1102 76-77 0.4270814360475086 1102 78-79 0.3868458031582307 1102 80-81 0.3505649192153726 1102 82-83 0.323983635632338 1102 84-85 0.3307840744317758 1102 86-87 0.3489609017425792 1102 88-89 0.3697118725049364 1102 90-91 0.4504761383096181 1102 92-93 0.41924129993105197 1102 94-95 0.43377312203544705 1102 96-97 0.4648202565524926 1102 98-99 0.47277196398592025 1102 100-101 0.5073315942514114 1102 102-103 0.49109643305631323 1102 104-105 0.49217284891516044 1102 106-107 0.5121119863056371 1102 108-109 0.4556950906061026 1102 110-111 0.4943974534491353 1102 112-113 0.4940123072239757 1102 114-115 0.5346383429246728 1102 116-117 0.5289603998993933 1102 118-119 0.5310877562030498 1102 120-121 0.568738611097281 1102 122-123 0.5271779594365604 1102 124-125 0.6647751359459306 1103 1 0.21174570162284212 1103 2 0.27808978480478075 1103 3 0.2720274759989145 1103 4 0.2336987199424243 1103 5 0.24838826029201755 1103 6 0.22428881769243958 1103 7 0.19532024814292726 1103 8 0.22572789584992137 1103 9 0.3063553201941005 1103 10-11 0.25273563824397627 1103 12-13 0.26878822996391705 1103 14-15 0.4326583040427394 1103 16-17 0.294590352525816 1103 18-19 0.3650899352662762 1103 20-21 0.357011991723148 1103 22-23 0.42311175552364233 1103 24-25 0.4238995805357959 1103 26-27 0.392029091104952 1103 28-29 0.42242270584979025 1103 30-31 0.3837363711687587 1103 32-33 0.1784421569638326 1103 34-35 0.4489411791095108 1103 36-37 0.5190938652245052 1103 38-39 0.5427270442310359 1103 40-41 0.518361076367647 1103 42-43 0.5240119231826341 1103 44-45 0.48722923177797384 1103 46-47 0.5142646756668583 1103 48-49 0.40966022539596025 1103 50-51 0.512254982963178 1103 52-53 0.5003326231641054 1103 54-55 0.46756209919844594 1103 56-57 0.5295953248805461 1103 58-59 0.5703308451320623 1103 60-61 0.5559549637537273 1103 62-63 0.5098935719262485 1103 64-65 0.5353714905372726 1103 66-67 0.535616249254268 1103 68-69 0.5582149010525157 1103 70-71 0.4838713462736308 1103 72-73 0.45529108881331126 1103 74-75 0.4623904715242375 1103 76-77 0.47201176792076893 1103 78-79 0.522134895444978 1103 80-81 0.44330800296705775 1103 82-83 0.38437579367891317 1103 84-85 0.31552973822357444 1103 86-87 0.3268484284964117 1103 88-89 0.3608634619364324 1103 90-91 0.2976643071553333 1103 92-93 0.3615531527198357 1103 94-95 0.33742744093605026 1103 96-97 0.34676007641256135 1103 98-99 0.3488614120847515 1103 100-101 0.40596489749769304 1103 102-103 0.41947526424984005 1103 104-105 0.4245799236990919 1103 106-107 0.4887923078647738 1103 108-109 0.4616731562401526 1103 110-111 0.4023948734090297 1103 112-113 0.36975660955154765 1103 114-115 0.39839964695180896 1103 116-117 0.37522110268863784 1103 118-119 0.48343399275569965 1103 120-121 0.5435713462114862 1103 122-123 0.46001309547732916 1103 124-125 0.4720137383369938 1104 1 0.3891623390394763 1104 2 0.4292211759361706 1104 3 0.40136174533318325 1104 4 0.3963733426170464 1104 5 0.4539738458776057 1104 6 0.4351066485102706 1104 7 0.3368365696592477 1104 8 0.377481727603751 1104 9 0.33699999083876975 1104 10-11 0.35599409150243844 1104 12-13 0.4209948521705371 1104 14-15 0.4994162308006693 1104 16-17 0.5877692457047061 1104 18-19 0.6492352494115963 1104 20-21 0.5498422245533803 1104 22-23 0.4176548700667553 1104 24-25 0.5410120176482351 1104 26-27 0.5205525496284125 1104 28-29 0.6025970360241146 1104 30-31 0.5927032601356501 1104 32-33 -0.17758568906401706 1104 34-35 0.43497955514789055 1104 36-37 0.5321791583098019 1104 38-39 0.6296616411656331 1104 40-41 0.6629264209329975 1104 42-43 0.6495471987179187 1104 44-45 0.6328896974384364 1104 46-47 0.621967963370146 1104 48-49 0.5648234405591737 1104 50-51 0.5324913231995225 1104 52-53 0.5974450602765415 1104 54-55 0.6399313515676965 1104 56-57 0.6921307174159352 1104 58-59 0.6867249015261194 1104 60-61 0.744071261870026 1104 62-63 0.742212514245189 1104 64-65 0.749695814861596 1104 66-67 0.766902080540099 1104 68-69 0.7763534291910474 1104 70-71 0.7703473027495846 1104 72-73 0.6223052458274765 1104 74-75 0.6432691424029002 1104 76-77 0.6355674014764006 1104 78-79 0.6326941560042343 1104 80-81 0.6114874811465327 1104 82-83 0.5985269578300745 1104 84-85 0.5731113458051809 1104 86-87 0.573269394917375 1104 88-89 0.592472213545193 1104 90-91 0.5840759135669344 1104 92-93 0.6364542876209782 1104 94-95 0.6215142550228663 1104 96-97 0.6171274567799401 1104 98-99 0.5993352625586006 1104 100-101 0.5685868701196668 1104 102-103 0.5229431477177258 1104 104-105 0.606895356014526 1104 106-107 0.6373216163940789 1104 108-109 0.5792255137925082 1104 110-111 0.6381875492017066 1104 112-113 0.6875429673379045 1104 114-115 0.6170775456297086 1104 116-117 0.6751286725962053 1104 118-119 0.6839218832435918 1104 120-121 0.6164600791002179 1104 122-123 0.7591332545974865 1104 124-125 0.8251949715182292 1105 1 0.15478828267101719 1105 2 0.19391368007410748 1105 3 0.12704002025083838 1105 4 0.14844469024677664 1105 5 0.17137981309786454 1105 6 0.19133582254153225 1105 7 -0.038771698948259825 1105 8 0.08943684956142306 1105 9 0.12667605885622635 1105 10-11 0.27465610870171986 1105 12-13 0.24589843243661846 1105 14-15 0.3053673287995906 1105 16-17 0.2990168381021405 1105 18-19 0.3238744311405739 1105 20-21 0.28728611874110044 1105 22-23 0.20907617326113836 1105 24-25 0.2937210537151955 1105 26-27 0.2588931787271136 1105 28-29 0.23782137667964776 1105 30-31 0.2887548249534362 1105 32-33 -0.0978050245491886 1105 34-35 0.30634644569819613 1105 36-37 0.20957680009817636 1105 38-39 0.279219996826761 1105 40-41 0.39648716189643096 1105 42-43 0.3884281219158012 1105 44-45 0.3250860586961082 1105 46-47 0.2220325845795159 1105 48-49 0.24989422324888722 1105 50-51 0.3672256857588607 1105 52-53 0.38907694403765447 1105 54-55 0.3719623274602881 1105 56-57 0.3861420558612636 1105 58-59 0.34104067987141207 1105 60-61 0.25424404761900377 1105 62-63 0.34929696121515974 1105 64-65 0.34836827188816244 1105 66-67 0.2924086269064716 1105 68-69 0.29400853165662966 1105 70-71 0.378678978705274 1105 72-73 0.34081126029126096 1105 74-75 0.3776711153981154 1105 76-77 0.3328417806205337 1105 78-79 0.22949728927392243 1105 80-81 0.31174870809054056 1105 82-83 0.2594517745549467 1105 84-85 0.29941095563314946 1105 86-87 0.2841064642332043 1105 88-89 0.23903397536515314 1105 90-91 0.24101208855958944 1105 92-93 0.2388581569645538 1105 94-95 0.2869620938795059 1105 96-97 0.2930001239245499 1105 98-99 0.2920244100938447 1105 100-101 0.3080337905358057 1105 102-103 0.3376773781273563 1105 104-105 0.33900327607716463 1105 106-107 0.3334407757897573 1105 108-109 0.26239254772524845 1105 110-111 0.19898692281206465 1105 112-113 0.27027291609685733 1105 114-115 0.3330992446124874 1105 116-117 0.41653643044507405 1105 118-119 0.4678951956091666 1105 120-121 0.37352305174192324 1105 122-123 0.357082687510033 1105 124-125 0.3482073902874525 1106 1 0.06011380795273169 1106 2 0.15649250333488496 1106 3 0.10282066857554639 1106 4 0.19911785924691827 1106 5 0.13032017470801094 1106 6 0.09934457618768988 1106 7 0.13555924825453758 1106 8 -0.04455063493816169 1106 9 0.07348312553846625 1106 10-11 0.12147733437421238 1106 12-13 0.17387248409275458 1106 14-15 0.20940604989876732 1106 16-17 0.16256801381558716 1106 18-19 0.22351358254342557 1106 20-21 0.2256121870749368 1106 22-23 0.18836880581253723 1106 24-25 0.26230912818100904 1106 26-27 0.19110785419645282 1106 28-29 0.1958550518935951 1106 30-31 0.20813311690308467 1106 32-33 0.1935735338786415 1106 34-35 0.23070917584566075 1106 36-37 0.18370476645961276 1106 38-39 0.19821773475357674 1106 40-41 0.2068955091695983 1106 42-43 0.21095649006351636 1106 44-45 0.2696525524178597 1106 46-47 0.2843524578565493 1106 48-49 0.19672160608791245 1106 50-51 0.16341861247075684 1106 52-53 0.18226775350687774 1106 54-55 0.19515389972018227 1106 56-57 0.24833285922317572 1106 58-59 0.365766751395995 1106 60-61 0.32548106226072093 1106 62-63 0.3027905985812396 1106 64-65 0.32795717133951285 1106 66-67 0.3222527807952602 1106 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88-89 -2.2291424420257755 2216 90-91 -2.238119795254981 2216 92-93 -2.3102693285686655 2216 94-95 -2.318291202464632 2216 96-97 -2.2457119812900785 2216 98-99 -2.250008557052208 2216 100-101 -2.2896729647057015 2216 102-103 -2.3522629528160444 2216 104-105 -2.238338273624443 2216 106-107 -2.218366558699433 2216 108-109 -2.284565254500688 2216 110-111 -2.3099699445989934 2216 112-113 -2.278345844497508 2216 114-115 -2.3672822450650344 2216 116-117 -2.449002479423296 2216 118-119 -2.5990481079302867 2216 120-121 -2.6512870524090495 2216 122-123 -2.6446173758400384 2216 124-125 -2.9374572791437323 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 50240.0 3 8478.0 4 4814.0 5 4037.0 6 4330.0 7 4979.0 8 5874.0 9 6650.0 10 7327.0 11 8210.0 12 8934.0 13 9089.0 14 9011.0 15 8281.0 16 7532.0 17 6826.0 18 6542.0 19 6703.0 20 6968.0 21 7725.0 22 8880.0 23 11704.0 24 15227.0 25 19567.0 26 24193.0 27 29122.0 28 34838.0 29 42908.0 30 51815.0 31 62615.0 32 80712.0 33 106598.0 34 147964.0 35 222232.0 36 422482.0 37 795330.0 38 371498.0 39 2043.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.38515124600325 18.03951774412896 12.639540569105288 24.935790440762506 2 15.8792471610534 19.252053880637916 39.82093473609647 25.047764222212216 3 19.232053575278986 23.33316668657523 29.387023611301398 28.047756126844387 4 12.730004457361973 15.424342386459388 36.71980900509239 35.125844151086255 5 13.816881965470007 37.504775477194684 34.73975032793836 13.93859222939694 6 33.955980378865846 35.44853080170549 16.861114982977575 13.73437383645109 7 29.04768541943121 31.69614984391259 21.026644645772276 18.229520090883923 8 26.37564874680639 35.590087492505276 19.53225307522217 18.50201068546616 9 26.096667365588377 15.683306732296561 19.699433109563806 38.52059279255125 10-11 25.39945264525824 25.83576263621079 27.931097247812957 20.83368747071801 12-13 25.490839902728823 23.2801000752398 27.8945214001747 23.334538621856677 14-15 22.519758781118192 25.25386686975762 25.069370098498855 27.157004250625334 16-17 22.984086719302287 28.16276788043289 25.932055672612712 22.92108972765211 18-19 22.979739818058274 27.422729003690094 27.123560640176375 22.47397053807526 20-21 23.49498372470186 26.735452496067722 27.07994185047199 22.689621928758424 22-23 23.138227873245434 26.66137599486595 26.908414078152965 23.291982053735655 24-25 22.977193549724014 26.83173250373238 27.1434822193035 23.047591727240103 26-27 22.81595742953708 27.080758391421732 26.887101749480237 23.216182429560952 28-29 22.841100728358587 27.32147709693122 26.910100869481525 22.92732130522867 30-31 23.09116690777318 26.963098767730877 26.752720206635445 23.193014117860503 32-33 22.691334145371666 27.060795545477013 26.916855448391452 23.331014860759872 34-35 23.316913944207435 27.090139600774517 26.71607697741664 22.876869477601407 36-37 23.360340640219764 26.868134762694794 26.6771659735419 23.09435862354354 38-39 22.86774853807307 27.02613332132512 26.808478079062258 23.297640061539553 40-41 23.272816776982975 26.905489230088207 26.891713206855954 22.92998078607286 42-43 23.165953760148543 26.942733076540687 26.646546895827232 23.244766267483545 44-45 22.997419669784144 27.060394917812445 26.836083096518397 23.106102315885014 46-47 23.20282730614658 27.19051147018622 26.504182063370102 23.102479160297097 48-49 23.11972037454419 27.12733021213176 26.65149585570398 23.10145355762007 50-51 23.07139160265835 27.14939319962198 26.651525283476314 23.12768991424336 52-53 23.26588277144252 27.051160060351304 26.63974674741497 23.0432104207912 54-55 23.03713575429783 26.987976084980296 26.727812850392212 23.247075310329667 56-57 22.8320614852291 27.30360697623217 26.62848896508488 23.235842573453855 58-59 23.04227566723309 27.0682198307981 26.70821498391847 23.18128951805033 60-61 22.840972146426463 27.06411672351667 26.903221940919252 23.19168918913762 62-63 22.875793917371524 27.09878545909799 26.92692906653638 23.09849155699411 64-65 22.845080867973564 27.129603459038897 26.69896029208542 23.32635538090212 66-67 22.690901789654177 27.251248604814858 26.670958467380533 23.386891138150432 68-69 22.929965849616533 26.911135895849636 26.92831511731404 23.230583137219796 70-71 22.788053256292375 26.783744897519007 26.761756463587954 23.66644538260066 72-73 22.745591670274575 26.999163117935467 26.76188109097046 23.493364120819503 74-75 22.71054440356147 27.180514568130658 26.681208679474405 23.427732348833466 76-77 22.867036925767874 27.033215287636658 26.689041524167557 23.41070626242791 78-79 22.81698846592751 27.154473189800417 26.751481225969094 23.277057118302984 80-81 22.885876216952084 26.96955166992816 26.802761186109773 23.34181092700998 82-83 22.83140035508084 27.074790119200802 26.854759108323705 23.239050417394647 84-85 22.79843352711599 27.112513745385357 26.73091646146014 23.35813626603851 86-87 22.830007401421575 27.03761441380092 26.75048964278121 23.381888541996293 88-89 22.795422557927093 27.064557614837824 26.693340927283977 23.446678899951102 90-91 22.69159997847352 27.109757182076994 26.768730422449522 23.429912416999965 92-93 22.81533536180419 26.838638858397367 26.96117534262888 23.384850437169565 94-95 22.63906614477107 26.752199838790442 27.16064318950672 23.448090826931768 96-97 22.754667083893025 26.555726431798753 27.09324245939675 23.596364024911466 98-99 22.494736773843588 26.602265657215685 26.9373993654756 23.965598203465127 100-101 22.462132706275835 26.553576091150425 27.21594976114381 23.76834144142993 102-103 22.34044866036948 26.42303680098253 27.27020125447926 23.96631328416873 104-105 22.317510375913006 26.51532576037582 27.06689598378455 24.10026787992663 106-107 22.3604315814086 26.55090748127079 27.03467626245038 24.053984674870225 108-109 22.362978248832352 26.418693526110765 26.993367474129755 24.22496075092713 110-111 22.261650070722112 26.540099457383775 27.173297985631223 24.024952486262894 112-113 22.3875730709935 26.61015646030476 27.113414118343293 23.888856350358445 114-115 22.19834875655023 26.77512295428319 27.045859882575108 23.98066840659147 116-117 22.042247623792914 26.638622905325793 27.240631610311766 24.078497860569527 118-119 22.091746845028535 26.661837135380566 27.05576963593782 24.190646383653082 120-121 21.984962428971702 26.849893928658457 27.16433911457289 24.00080452779695 122-123 22.033407506531088 26.786342085007636 27.06729095509835 24.112959453362926 124-125 22.065027382973014 26.748701438314338 27.06514089444368 24.121130284268972 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 10655.0 1 5591.0 2 511.0 3 481.5 4 407.0 5 330.5 6 291.5 7 265.0 8 280.5 9 288.5 10 281.0 11 278.5 12 278.0 13 301.5 14 357.5 15 402.0 16 439.5 17 574.0 18 799.0 19 1037.0 20 1373.0 21 2022.5 22 2902.0 23 4230.0 24 6136.5 25 8514.5 26 12038.5 27 16519.5 28 20780.5 29 24986.0 30 29382.5 31 34089.5 32 41147.0 33 46118.5 34 48716.5 35 52952.0 36 58389.0 37 63323.5 38 66494.0 39 69242.0 40 73094.5 41 78506.0 42 81980.5 43 84751.0 44 88638.0 45 92878.5 46 95525.0 47 96946.0 48 98326.0 49 98180.5 50 96043.5 51 94661.0 52 95697.0 53 95790.0 54 96196.0 55 92127.0 56 84375.0 57 77664.0 58 71729.5 59 63949.5 60 53342.0 61 44771.0 62 37180.5 63 28646.5 64 20848.5 65 15354.0 66 11415.0 67 9168.5 68 7074.0 69 5296.0 70 4091.5 71 2808.5 72 2114.5 73 1624.5 74 1139.0 75 791.5 76 525.0 77 358.0 78 223.5 79 125.5 80 93.0 81 64.0 82 43.0 83 35.5 84 32.5 85 30.5 86 29.5 87 21.5 88 18.0 89 16.5 90 14.0 91 11.0 92 6.5 93 6.0 94 6.5 95 4.5 96 2.0 97 2.0 98 2.0 99 2.0 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.46883345907992996 2 0.4602857296987628 3 0.4545492535362906 4 0.45185197004267785 5 0.46047567924056654 6 0.5194360170164397 7 0.5148772280131506 8 0.5230450583107104 9 0.5303011308076122 10-11 0.5476055340659308 12-13 0.5564571827139838 14-15 0.5347079601774585 16-17 0.5280217363059677 18-19 0.5389628299138617 20-21 0.5394566987225513 22-23 0.5494480446214267 24-25 0.543730563413135 26-27 0.5508726661849546 28-29 0.5316117826460579 30-31 0.5371962991750872 32-33 0.5385069510135327 34-35 0.536930369816562 36-37 0.48399143251586646 38-39 0.4501614191206248 40-41 0.4476350902146354 42-43 0.4610265329117973 44-45 0.4659272310903332 46-47 0.4710368737648531 48-49 0.4852830894001317 50-51 0.46750381228730403 52-53 0.4667060242117284 54-55 0.45587890032891665 56-57 0.438821431474943 58-59 0.457227542075723 60-61 0.4532955865603861 62-63 0.46947928752206264 64-65 0.46953627238460377 66-67 0.4769253095607683 68-69 0.48758147885595665 70-71 0.4831936444402909 72-73 0.49508448575720343 74-75 0.48404841737840754 76-77 0.48137012883897523 78-79 0.493678859147856 80-81 0.4871825848181689 82-83 0.4891010751903864 84-85 0.4848462054539832 86-87 0.4752917435012563 88-89 0.48053435085503887 90-91 0.4653383875107417 92-93 0.4650724581522165 94-95 0.45914603244794056 96-97 0.44820493884004653 98-99 0.4097971414873353 100-101 0.4073088024897066 102-103 0.397868310262062 104-105 0.40197122036502225 106-107 0.4015913212814148 108-109 0.41327321810234335 110-111 0.4352503800890331 112-113 0.4351174154097705 114-115 0.4286591309884442 116-117 0.42307461445941497 118-119 0.46674401412008915 120-121 0.4599628154776965 122-123 0.42398637226007285 124-125 0.43298998054156895 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2632278.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 74.30970534409835 #Duplication Level Percentage of deduplicated Percentage of total 1 84.24610163305734 62.60302988741453 2 10.040211966333935 14.921703856211298 3 2.886147857880879 6.434063906958863 4 1.1502784977790255 3.4190742493444586 5 0.5559833332542141 2.0657478835175143 6 0.3177168994483211 1.416566950850716 7 0.1874523320782278 0.9750669293958028 8 0.1250782535351907 0.7435622532123559 9 0.087374835276666 0.5843518437508362 >10 0.38333582610077926 4.717424887674436 >50 0.015890503533317266 0.7980152257758489 >100 0.00422679346288009 0.47636767090329857 >500 1.006341296524829E-4 0.041054727945976505 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 1.006341296524829E-4 0.8039697270440682 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 10751 0.40842950478634854 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 7.597981672148611E-5 0.0 0.0 0.0 6 0.0 7.597981672148611E-5 0.0 0.0 0.0 7 0.0 1.1396972508222915E-4 0.0 0.0 0.0 8 0.0 1.1396972508222915E-4 0.0 0.0 0.0 9 0.0 1.1396972508222915E-4 0.0 0.0 0.0 10-11 0.0 1.1396972508222915E-4 0.0 3.7989908360743054E-5 0.0 12-13 0.0 1.3296467926260069E-4 0.0 3.7989908360743054E-5 7.597981672148611E-5 14-15 0.0 1.5195963344297222E-4 0.0 3.7989908360743054E-5 7.597981672148611E-5 16-17 0.0 1.5195963344297222E-4 0.0 3.7989908360743054E-5 7.597981672148611E-5 18-19 0.0 1.5195963344297222E-4 0.0 5.698486254111458E-5 7.597981672148611E-5 20-21 0.0 2.279394501644583E-4 0.0 1.1396972508222915E-4 7.597981672148611E-5 22-23 0.0 2.279394501644583E-4 0.0 1.5195963344297222E-4 7.597981672148611E-5 24-25 0.0 2.279394501644583E-4 0.0 1.8994954180371525E-4 7.597981672148611E-5 26-27 0.0 2.279394501644583E-4 0.0 6.078385337718889E-4 7.597981672148611E-5 28-29 0.0 2.279394501644583E-4 0.0 0.0014246215635278644 7.597981672148611E-5 30-31 0.0 2.279394501644583E-4 0.0 0.005052657811978826 7.597981672148611E-5 32-33 0.0 2.279394501644583E-4 0.0 0.01384732159749084 7.597981672148611E-5 34-35 0.0 2.279394501644583E-4 0.0 0.027580673469899456 7.597981672148611E-5 36-37 0.0 2.6592935852520137E-4 0.0 0.052027179500037615 7.597981672148611E-5 38-39 0.0 3.0391926688594443E-4 0.0 0.08695890023774085 7.597981672148611E-5 40-41 0.0 3.0391926688594443E-4 0.0 0.12358117189749715 7.597981672148611E-5 42-43 0.0 3.419091752466875E-4 0.0 0.1623498733796354 7.597981672148611E-5 44-45 0.0 3.419091752466875E-4 0.0 0.21135685516499397 7.597981672148611E-5 46-47 0.0 3.419091752466875E-4 0.0 0.2739072392809574 7.597981672148611E-5 48-49 0.0 3.419091752466875E-4 0.0 0.34114937707947257 7.597981672148611E-5 50-51 0.0 3.419091752466875E-4 0.0 0.41509673370365896 7.597981672148611E-5 52-53 0.0 3.419091752466875E-4 0.0 0.4885312265649753 7.597981672148611E-5 54-55 0.0 4.558789003289166E-4 0.0 0.5686519432977823 7.597981672148611E-5 56-57 0.0 5.318587170504027E-4 0.0 0.6743778582657303 7.597981672148611E-5 58-59 0.0 5.698486254111458E-4 0.0 0.7941790342813335 7.597981672148611E-5 60-61 0.0 5.698486254111458E-4 0.0 0.9226609043573666 7.597981672148611E-5 62-63 0.0 5.698486254111458E-4 0.0 1.051066794616678 7.597981672148611E-5 64-65 0.0 5.698486254111458E-4 0.0 1.1868807170063345 7.597981672148611E-5 66-67 0.0 5.698486254111458E-4 0.0 1.350047373415726 7.597981672148611E-5 68-69 0.0 5.698486254111458E-4 0.0 1.5443467597267462 7.597981672148611E-5 70-71 0.0 5.698486254111458E-4 0.0 1.7537851245195228 7.597981672148611E-5 72-73 0.0 5.698486254111458E-4 0.0 1.971144385205514 1.3296467926260069E-4 74-75 0.0 5.698486254111458E-4 0.0 2.193024444986434 1.8994954180371525E-4 76-77 0.0 5.698486254111458E-4 0.0 2.4374515153794545 1.8994954180371525E-4 78-79 0.0 5.888435795915173E-4 0.0 2.7218439693679772 1.8994954180371525E-4 80-81 0.0 7.59798167214861E-4 0.0 3.042307841345025 1.8994954180371525E-4 82-83 0.0 7.59798167214861E-4 0.0 3.379278328504816 2.279394501644583E-4 84-85 0.0 7.59798167214861E-4 0.0 3.716856654198379 2.279394501644583E-4 86-87 0.0 7.977880755756041E-4 0.0 4.073012045080345 2.6592935852520137E-4 88-89 0.0 7.977880755756041E-4 0.0 4.4681070920320725 3.0391926688594443E-4 90-91 0.0 7.977880755756041E-4 0.0 4.907878271216035 3.0391926688594443E-4 92-93 0.0 7.977880755756041E-4 0.0 5.360831948601174 3.419091752466875E-4 94-95 0.0 7.977880755756041E-4 0.0 5.811734170934833 3.419091752466875E-4 96-97 0.0 7.977880755756041E-4 0.0 6.278611149734185 3.60904129427059E-4 98-99 0.0 8.357779839363471E-4 0.0 6.773809605216471 3.798990836074305E-4 100-101 0.0 8.357779839363471E-4 0.0 7.336060248955468 3.798990836074305E-4 102-103 0.0 8.737678922970902E-4 0.0 7.927981011124205 3.798990836074305E-4 104-105 0.0 8.737678922970902E-4 0.0 8.522010213206965 3.798990836074305E-4 106-107 0.0 8.737678922970902E-4 0.0 9.103312795988874 3.798990836074305E-4 108-109 0.0 9.117578006578332E-4 0.0 9.715577154084789 3.798990836074305E-4 110-111 0.0 9.117578006578332E-4 0.0 10.379127888467707 3.798990836074305E-4 112-113 0.0 9.117578006578332E-4 0.0 11.082017172958174 3.798990836074305E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2405 0.0 50.977364 1 GTATCAA 3835 0.0 41.59055 1 ATCAACG 4150 0.0 36.43012 3 TCAACGC 4165 0.0 36.30169 4 CAACGCA 4180 0.0 36.031765 5 AACGCAG 4415 0.0 34.113876 6 TATCAAC 4675 0.0 33.35313 2 TACATGG 8490 0.0 32.175247 2 ACATGGG 8080 0.0 31.455128 3 GTACATG 8920 0.0 30.891666 1 ACGCAGA 5405 0.0 27.757963 7 CATGGGG 5635 0.0 27.359865 4 CGCAGAG 5500 0.0 26.737268 8 GTGTAGC 1760 0.0 26.037739 1 GAGTACA 6075 0.0 23.414066 1 AGAGTAC 5615 0.0 20.728868 10-11 TAGGTCG 150 0.0046934313 19.84358 5 CATGGGA 3430 0.0 19.61067 4 GTACCGT 365 2.575689E-9 19.57175 6 AGTACAT 6670 0.0 19.54048 2 >>END_MODULE