FastQCFastQC Report
Thu 26 May 2016
SRR1364123_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364123_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2632278
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49300.18729024821846327No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48380.1837951766492749No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA39050.069.144251
GTATCAA66050.049.3620721
TCAACGC83300.038.925074
ATCAACG83050.038.8989753
CAACGCA85050.038.124155
TATCAAC86250.037.595152
AACGCAG89400.036.6018566
CGTATAC1451.0995973E-732.8246353
ACGCAGA104900.030.9135197
CGCAGAG107400.030.1939338
TACATGG80850.028.3318632
GTACATG84400.027.7542171
ACATGGG78500.027.6631183
GTGGTAT19850.025.5233271
GCAGAGT129500.024.7195159
AGAGTAC111250.024.49587210-11
GAGTACT87300.024.4003612-13
CAGAGTA119000.024.05058110-11
CATGGGG54500.023.9070264
TGGTATC19200.023.860742
Produced by FastQC (version 0.11.5)