FastQCFastQC Report
Thu 26 May 2016
SRR1364122_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364122_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1399317
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN56550.4041257270511257No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA15950.11398417942467647No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA25550.064.754671
GTATCAA44900.048.3797651
GTACATG114550.040.368551
ATCAACG54150.038.586473
TCAACGC55000.037.9955944
TACATGG120650.037.984972
GTCGACG401.1991928E-437.180733112-113
CAACGCA56150.037.005355
TATCAAC58850.036.3075032
ACATGGG120650.036.0675353
AACGCAG59200.034.4966436
ATGGGAG35800.033.427345
CATGGGA89250.032.687124
TGGTATC13650.032.2661742
GTGGTAT14150.031.9649751
ACGCAGA71750.028.2137897
CATGGGG32050.028.0503144
CGCAGAG71750.028.0478258
CATGGGT10300.027.7455184
ATGGGAT28850.026.8278795
Produced by FastQC (version 0.11.5)