FastQCFastQC Report
Thu 26 May 2016
SRR1364122_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364122_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1399317
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83990.6002213937227947No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80690.5766384600487238No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44520.31815521429383053No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25670.18344663861012195No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14470.10340759098903252No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA42900.081.286461
GTGGTAT13600.056.9802171
GTATCAA83250.056.4237021
TGGTATC14700.052.2136762
TATCAAC111450.042.0685962
ATCAACG111450.042.065593
TCAACGC112850.041.543734
CAACGCA114050.041.1066175
AACGCAG118350.039.7653276
GTACATG97800.034.559211
ACGCAGA138150.033.8100787
CGCAGAG138700.033.5473068
TACATGG103650.032.54812
GCAGAGT148150.031.166469
ACATGGG102700.030.7033863
GAGTACT94300.028.14121212-13
CATGGGA77450.028.1151564
ATGGGAG31450.027.2409785
CAGAGTA147550.026.69610-11
GTATTGG6100.026.3847831
Produced by FastQC (version 0.11.5)