##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364122_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1399317 Sequences flagged as poor quality 0 Sequence length 125 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.01117259348668 33.0 31.0 34.0 30.0 34.0 2 32.043209651565725 33.0 31.0 34.0 30.0 34.0 3 32.16513985036986 34.0 31.0 34.0 30.0 34.0 4 35.82514040778465 37.0 35.0 37.0 35.0 37.0 5 35.78065942170359 37.0 35.0 37.0 35.0 37.0 6 35.83795451638192 37.0 35.0 37.0 35.0 37.0 7 35.76293148728987 37.0 35.0 37.0 33.0 37.0 8 35.756434031745485 37.0 35.0 37.0 33.0 37.0 9 37.3823007938873 39.0 37.0 39.0 34.0 39.0 10-11 37.30682861710392 39.0 37.0 39.0 33.5 39.0 12-13 37.25230666103535 39.0 37.0 39.0 33.0 39.0 14-15 38.49631427332048 40.0 38.0 41.0 33.0 41.0 16-17 38.488453295429125 40.0 38.0 41.0 33.5 41.0 18-19 38.67185634134367 40.0 38.0 41.0 34.0 41.0 20-21 38.74712449001906 40.0 38.0 41.0 34.0 41.0 22-23 38.707726340779104 40.0 38.0 41.0 34.0 41.0 24-25 38.66850113305277 40.0 38.0 41.0 34.0 41.0 26-27 38.52847603509426 40.0 38.0 41.0 34.0 41.0 28-29 38.432572819454066 40.0 38.0 41.0 34.0 41.0 30-31 38.23401988255699 40.0 37.5 41.0 34.0 41.0 32-33 38.02672375165884 40.0 37.0 41.0 33.0 41.0 34-35 37.861817586722665 40.0 37.0 41.0 33.0 41.0 36-37 37.70884581549427 40.0 37.0 41.0 33.0 41.0 38-39 37.57023319233598 40.0 36.0 41.0 33.0 41.0 40-41 37.38421279810079 40.0 36.0 41.0 32.5 41.0 42-43 37.169584876050244 40.0 36.0 41.0 31.5 41.0 44-45 36.89401722411719 40.0 36.0 41.0 31.0 41.0 46-47 36.46100704843863 40.0 35.5 41.0 30.0 41.0 48-49 36.039713660307136 40.0 35.0 41.0 28.5 41.0 50-51 35.80119301059017 39.5 35.0 41.0 28.0 41.0 52-53 35.62877853981621 39.0 35.0 41.0 27.0 41.0 54-55 35.449811943969806 39.0 35.0 41.0 27.0 41.0 56-57 35.257666418688544 39.0 35.0 41.0 26.5 41.0 58-59 35.054075309597465 38.5 35.0 41.0 26.0 41.0 60-61 34.820062573384014 38.0 34.5 40.5 26.0 41.0 62-63 34.571739998870875 38.0 34.0 40.0 26.0 41.0 64-65 34.36121372069374 37.0 34.0 40.0 26.0 41.0 66-67 34.1631085022193 37.0 34.0 40.0 26.0 41.0 68-69 33.863866086097715 36.0 34.0 39.0 26.0 41.0 70-71 33.555876188168945 36.0 34.0 39.0 25.0 41.0 72-73 33.2380086142025 35.5 34.0 39.0 25.0 40.5 74-75 32.96960731556895 35.0 33.5 37.5 25.0 40.0 76-77 32.77455179919918 35.0 34.0 37.0 25.0 39.0 78-79 32.49365011644967 35.0 33.0 37.0 25.0 39.0 80-81 32.23081153162579 35.0 33.0 36.0 25.0 39.0 82-83 31.975112858630318 35.0 33.0 36.0 24.0 37.0 84-85 31.769030176864856 35.0 33.0 35.5 24.0 37.0 86-87 31.58442868913906 35.0 33.0 35.0 24.0 36.5 88-89 31.43889304567871 35.0 33.0 35.0 23.5 36.0 90-91 31.307267045279946 35.0 33.0 35.0 23.0 36.0 92-93 31.206367106238257 35.0 33.0 35.0 23.0 36.0 94-95 31.119582982269208 35.0 33.0 35.0 22.0 36.0 96-97 31.053411771600004 35.0 33.0 35.0 20.0 35.0 98-99 30.989326221292245 35.0 33.0 35.0 20.0 35.0 100-101 30.930383537111318 35.0 33.0 35.0 20.0 35.0 102-103 29.76766129476023 33.5 30.0 34.5 18.5 35.0 104-105 30.679908841241833 35.0 32.0 35.0 19.0 35.0 106-107 30.825446628605242 35.0 33.0 35.0 19.0 35.0 108-109 30.811083192729022 35.0 33.0 35.0 19.0 35.0 110-111 30.77165002640574 35.0 33.0 35.0 18.5 35.0 112-113 30.704351480043478 35.0 33.0 35.0 17.5 35.0 114-115 30.625462636414767 35.0 33.0 35.0 16.0 35.0 116-117 30.557049260460637 35.0 33.0 35.0 8.5 35.0 118-119 30.46353578209941 35.0 33.0 35.0 2.0 35.0 120-121 30.338873178843677 35.0 33.0 35.0 2.0 35.0 122-123 30.186922262789633 35.0 33.0 35.0 2.0 35.0 124-125 29.34569257716443 34.5 31.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3277346177408411 1101 2 0.4813763159491167 1101 3 0.34528733643291787 1101 4 0.32163540235092114 1101 5 0.3498117049762115 1101 6 0.4493511989613026 1101 7 0.4295274521576289 1101 8 0.3537620374929489 1101 9 0.46530675272460087 1101 10-11 0.4406943676390611 1101 12-13 0.4078322565818695 1101 14-15 0.5643686051172025 1101 16-17 0.6371050460391103 1101 18-19 0.6208094405230469 1101 20-21 0.6243179788986168 1101 22-23 0.6472976861015667 1101 24-25 0.5953155564292274 1101 26-27 0.5708965285778476 1101 28-29 0.6360788608635133 1101 30-31 0.7107196698429092 1101 32-33 0.6657587365919255 1101 34-35 0.658672232614272 1101 36-37 0.6945568028446729 1101 38-39 0.7320163418906276 1101 40-41 0.7008608842015605 1101 42-43 0.7169172285132888 1101 44-45 0.6734105183830152 1101 46-47 0.7238195723416609 1101 48-49 0.8160127842789038 1101 50-51 0.7723178366080177 1101 52-53 0.7923536382994314 1101 54-55 0.8463695310969328 1101 56-57 0.763848381883939 1101 58-59 0.7085496095942503 1101 60-61 0.724296182854907 1101 62-63 0.6376213774277844 1101 64-65 0.6798366971235197 1101 66-67 0.6937932098655182 1101 68-69 0.6635243503316772 1101 70-71 0.747027617654318 1101 72-73 0.7509488889714646 1101 74-75 0.731843823734458 1101 76-77 0.7468769822167971 1101 78-79 0.7561378047808773 1101 80-81 0.7908996254116119 1101 82-83 0.7897257708920549 1101 84-85 0.7333071523949819 1101 86-87 0.7674659395147216 1101 88-89 0.7484610789606485 1101 90-91 0.7476672840278482 1101 92-93 0.7171432932286805 1101 94-95 0.7565140807858093 1101 96-97 0.7744360694858834 1101 98-99 0.6820866245494344 1101 100-101 0.6878650326650764 1101 102-103 0.736444608315999 1101 104-105 0.7460658060279854 1101 106-107 0.7212046112622161 1101 108-109 0.7538510812940906 1101 110-111 0.7211710117540058 1101 112-113 0.7213218848406626 1101 114-115 0.7635211814118108 1101 116-117 0.7213772654602053 1101 118-119 0.7193467137092782 1101 120-121 0.7780463465001795 1101 122-123 0.7439753291928213 1101 124-125 0.766104797199695 1102 1 0.40019146942480077 1102 2 0.4066201794862643 1102 3 0.34065896599538803 1102 4 0.27240602791755464 1102 5 0.3208754704642729 1102 6 0.36475111837300744 1102 7 0.23062798605507595 1102 8 0.22895595307670646 1102 9 0.41931434145567437 1102 10-11 0.3410301521996857 1102 12-13 0.41852657510710856 1102 14-15 0.38444831197728035 1102 16-17 0.38691155018604206 1102 18-19 0.520551725872771 1102 20-21 0.5119546012417189 1102 22-23 0.507586334904488 1102 24-25 0.4055299128981176 1102 26-27 0.48543306193803915 1102 28-29 0.5364912462770306 1102 30-31 0.5242389858497631 1102 32-33 0.46364875035909137 1102 34-35 0.48013306334527783 1102 36-37 0.6209752827479704 1102 38-39 0.54102502192152 1102 40-41 0.5443158135189066 1102 42-43 0.5765506776923033 1102 44-45 0.5544135068656075 1102 46-47 0.48898549188768214 1102 48-49 0.5986523027638455 1102 50-51 0.6533884017334302 1102 52-53 0.5714999480271103 1102 54-55 0.6674542831200299 1102 56-57 0.7058705604541728 1102 58-59 0.656682437571817 1102 60-61 0.6072240563312761 1102 62-63 0.6763782610112798 1102 64-65 0.6293384767816974 1102 66-67 0.5792952917297924 1102 68-69 0.620241090870941 1102 70-71 0.6507212478211954 1102 72-73 0.585431864694236 1102 74-75 0.5334723788534887 1102 76-77 0.54902957111576 1102 78-79 0.5994456184876 1102 80-81 0.5354674706820859 1102 82-83 0.49333671411088176 1102 84-85 0.509587750763064 1102 86-87 0.5097609430404617 1102 88-89 0.43687819054185795 1102 90-91 0.4560761688873214 1102 92-93 0.43661250178447375 1102 94-95 0.3951792532111824 1102 96-97 0.3998547508599195 1102 98-99 0.4802192426616543 1102 100-101 0.4957209223262673 1102 102-103 0.4596252855934573 1102 104-105 0.45064317918537 1102 106-107 0.39430365091837416 1102 108-109 0.4338458766334021 1102 110-111 0.3759120798846922 1102 112-113 0.4004318878627231 1102 114-115 0.46634408023992435 1102 116-117 0.46295075191130053 1102 118-119 0.4613369927462472 1102 120-121 0.44170207498478575 1102 122-123 0.47307043177499963 1102 124-125 0.5763437079145781 1103 1 0.3358718488584209 1103 2 0.4144448102075664 1103 3 0.3456734758474411 1103 4 0.24827295693607 1103 5 0.2939892886734441 1103 6 0.29701010240623305 1103 7 0.2622178936908597 1103 8 0.3238978938733439 1103 9 0.35612027366912713 1103 10-11 0.2736476265748564 1103 12-13 0.2925666088380581 1103 14-15 0.3574999733850319 1103 16-17 0.4211520297039897 1103 18-19 0.391628622150094 1103 20-21 0.44052673678314136 1103 22-23 0.5156817634103135 1103 24-25 0.4081622732333656 1103 26-27 0.4333399217526761 1103 28-29 0.47655981671018566 1103 30-31 0.5686375495129781 1103 32-33 0.5802157154047975 1103 34-35 0.5984627873611572 1103 36-37 0.5796459585943055 1103 38-39 0.670648478393943 1103 40-41 0.766628917518716 1103 42-43 0.732853060633623 1103 44-45 0.6945329508591627 1103 46-47 0.7535735348194308 1103 48-49 0.7732119594366011 1103 50-51 0.8737552456327649 1103 52-53 0.7782049645164761 1103 54-55 0.6942306017745992 1103 56-57 0.6604984708375312 1103 58-59 0.7194716943427508 1103 60-61 0.7624208796040506 1103 62-63 0.7751446266595536 1103 64-65 0.7507225939373683 1103 66-67 0.712677651075289 1103 68-69 0.7208741400777541 1103 70-71 0.7001248196598127 1103 72-73 0.747522154376604 1103 74-75 0.6407391819747872 1103 76-77 0.5818145125418894 1103 78-79 0.5308749873783185 1103 80-81 0.5087671654407231 1103 82-83 0.5909650560287147 1103 84-85 0.584671781215949 1103 86-87 0.46527646838420367 1103 88-89 0.46444037703601637 1103 90-91 0.5209205591370178 1103 92-93 0.5367595798209521 1103 94-95 0.48658241331047236 1103 96-97 0.4835548225183963 1103 98-99 0.5635701277032439 1103 100-101 0.557049692215454 1103 102-103 0.5468087115021554 1103 104-105 0.620925299801236 1103 106-107 0.5806154959460272 1103 108-109 0.39591399573692243 1103 110-111 0.524499695240987 1103 112-113 0.5365693375335283 1103 114-115 0.5953756614376893 1103 116-117 0.6720080272263402 1103 118-119 0.636310566208067 1103 120-121 0.5858894242941339 1103 122-123 0.6367690781819846 1103 124-125 0.7481563902774866 1104 1 0.25619175465303456 1104 2 0.31502279951132905 1104 3 0.22548604637881908 1104 4 0.20320843605878736 1104 5 0.11771432669905835 1104 6 0.17410151107116434 1104 7 0.2103951175758496 1104 8 0.18940498381005 1104 9 0.2942871938398781 1104 10-11 0.23304559527119295 1104 12-13 0.26345959740584135 1104 14-15 0.2997103654168214 1104 16-17 0.35327753875654366 1104 18-19 0.3409613339895543 1104 20-21 0.3015355194728997 1104 22-23 0.2978818517278654 1104 24-25 0.2986681365374224 1104 26-27 0.34067076911051686 1104 28-29 0.31151737522056777 1104 30-31 0.38715381466189314 1104 32-33 0.3471309796214683 1104 34-35 0.3831936641581777 1104 36-37 0.3380071773765039 1104 38-39 0.30920007056311505 1104 40-41 0.3997617373018514 1104 42-43 0.5221965668403854 1104 44-45 0.46686453419658136 1104 46-47 0.4709289148302247 1104 48-49 0.44432426992185725 1104 50-51 0.45193394147693056 1104 52-53 0.37821870280212977 1104 54-55 0.47201039668161826 1104 56-57 0.32111325912072886 1104 58-59 0.3859679408468537 1104 60-61 0.45144349871009126 1104 62-63 0.510414240515594 1104 64-65 0.5218206754839088 1104 66-67 0.4858708211847045 1104 68-69 0.4158459275268527 1104 70-71 0.34176752610208894 1104 72-73 0.42008434465186184 1104 74-75 0.3554101990985785 1104 76-77 0.34451899220432125 1104 78-79 0.35695761335349374 1104 80-81 0.2759370785951347 1104 82-83 0.279239919872488 1104 84-85 0.3394026580129612 1104 86-87 0.38229427909025304 1104 88-89 0.303650425610666 1104 90-91 0.34030773346604803 1104 92-93 0.3831930227334688 1104 94-95 0.3562571103322085 1104 96-97 0.37508369745092907 1104 98-99 0.3798612812285853 1104 100-101 0.33245668892807956 1104 102-103 0.32335107939382013 1104 104-105 0.43031934327309784 1104 106-107 0.36245201034669705 1104 108-109 0.30381154737815663 1104 110-111 0.22827723314393467 1104 112-113 0.3279323717320466 1104 114-115 0.39025079023411635 1104 116-117 0.4738366911780574 1104 118-119 0.4212224939839331 1104 120-121 0.42770042473571834 1104 122-123 0.46375008990859 1104 124-125 0.5425987630756808 1105 1 0.1414804971280823 1105 2 0.2330609194007316 1105 3 0.17120599068926623 1105 4 0.1697156815830283 1105 5 0.19579774337062616 1105 6 0.15730640547671726 1105 7 0.16275898211690532 1105 8 0.14672306134402646 1105 9 0.2285605824705783 1105 10-11 0.29958519563984254 1105 12-13 0.21993958642480038 1105 14-15 0.2739126714348288 1105 16-17 0.35412317673524996 1105 18-19 0.30002216109702573 1105 20-21 0.352310270928875 1105 22-23 0.4206603485021816 1105 24-25 0.3863556475674841 1105 26-27 0.40050919097846815 1105 28-29 0.3909908736616501 1105 30-31 0.4246994546014946 1105 32-33 0.4709240458802668 1105 34-35 0.5861688391684723 1105 36-37 0.6126523525828915 1105 38-39 0.6328041060946177 1105 40-41 0.65404708742048 1105 42-43 0.6942332552730335 1105 44-45 0.7650432505916669 1105 46-47 0.663640446881125 1105 48-49 0.7663254660706471 1105 50-51 0.7683693200286115 1105 52-53 0.7188576619565907 1105 54-55 0.6774389513024772 1105 56-57 0.6685483043194651 1105 58-59 0.7545005015857171 1105 60-61 0.6202531345710156 1105 62-63 0.7110591804140185 1105 64-65 0.697538364759744 1105 66-67 0.7358404272399639 1105 68-69 0.7693865279543246 1105 70-71 0.7506731480014182 1105 72-73 0.7404849564525477 1105 74-75 0.743328213883764 1105 76-77 0.6422921945495474 1105 78-79 0.6134627999330959 1105 80-81 0.6350095730555836 1105 82-83 0.6604185186135894 1105 84-85 0.6172294909639184 1105 86-87 0.6108850785490461 1105 88-89 0.6989652480512056 1105 90-91 0.7001105650051613 1105 92-93 0.7055498280357426 1105 94-95 0.7222378542975392 1105 96-97 0.6577439519757071 1105 98-99 0.7850898240840465 1105 100-101 0.6983568399173556 1105 102-103 0.648116849929341 1105 104-105 0.685821235541269 1105 106-107 0.7202471747681329 1105 108-109 0.7348914148213375 1105 110-111 0.7176745506331983 1105 112-113 0.7289135078773157 1105 114-115 0.6581680206594385 1105 116-117 0.5317030362571806 1105 118-119 0.5923324612369107 1105 120-121 0.6731658442702013 1105 122-123 0.5546368087351894 1105 124-125 0.5979498621599433 1106 1 0.18728434761131396 1106 2 0.20404197369824928 1106 3 0.14055762869354282 1106 4 0.07884102625250478 1106 5 0.07491422401545833 1106 6 0.1596665670534705 1106 7 0.2251874794960358 1106 8 0.19806002616703466 1106 9 0.16172997735520767 1106 10-11 0.20899808436106326 1106 12-13 0.18128714820460345 1106 14-15 0.3261180192941211 1106 16-17 0.3451023447437862 1106 18-19 0.3491128317877781 1106 20-21 0.30996653386835504 1106 22-23 0.30567454934441685 1106 24-25 0.2800276081118369 1106 26-27 0.3189885353473585 1106 28-29 0.36512226059012676 1106 30-31 0.41544897076070697 1106 32-33 0.5178148128791094 1106 34-35 0.4085436769936308 1106 36-37 0.36301389130395734 1106 38-39 0.4778431660786069 1106 40-41 0.6129658604431825 1106 42-43 0.48180567803486696 1106 44-45 0.5415157061718645 1106 46-47 0.5307644236076925 1106 48-49 0.5297967131990404 1106 50-51 0.5419502326244228 1106 52-53 0.5722155630537031 1106 54-55 0.580392833837287 1106 56-57 0.6580153740078813 1106 58-59 0.63335474205536 1106 60-61 0.6330005374731442 1106 62-63 0.5184165508965108 1106 64-65 0.6289246031314661 1106 66-67 0.6227727386033948 1106 68-69 0.640763089510223 1106 70-71 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-2.6609317232774465 2216 110-111 -2.863441249354736 2216 112-113 -2.631121563729547 2216 114-115 -2.748677981579604 2216 116-117 -3.051890624709092 2216 118-119 -3.375273787073194 2216 120-121 -3.587714845229325 2216 122-123 -3.673111323721301 2216 124-125 -3.648768110278848 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 9.0 3 11.0 4 45.0 5 131.0 6 404.0 7 920.0 8 1654.0 9 2844.0 10 4681.0 11 6694.0 12 8877.0 13 12995.0 14 19871.0 15 17572.0 16 7082.0 17 5192.0 18 4885.0 19 4181.0 20 3983.0 21 4038.0 22 4203.0 23 4896.0 24 5982.0 25 7792.0 26 10252.0 27 13103.0 28 17010.0 29 21682.0 30 27408.0 31 34739.0 32 44224.0 33 57754.0 34 78012.0 35 116319.0 36 218635.0 37 424415.0 38 205818.0 39 1004.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.454625631392666 19.414322448371838 14.19230416805067 21.93874775218482 2 16.626062770360132 21.198092462539957 38.75348301389776 23.422361753202157 3 19.406324656957644 26.486707443702894 30.216026818798024 23.890941080541435 4 12.843408605769815 17.29315087289013 36.75014310552934 33.11329741581071 5 12.442355806439856 39.33483263620752 33.7016558792611 14.521155678091526 6 30.810173820513864 37.2832603334341 16.988287857576232 14.918277988475806 7 27.29560206872353 34.667126891190485 20.587972560899352 17.44929847918663 8 26.999100275348614 34.520269531492865 19.22773753195309 19.252892661205433 9 27.451892601890783 15.267448333722808 19.96538311190388 37.31527595248254 10-11 25.067640286597577 27.15502024563465 26.637945450413557 21.139394017354217 12-13 26.397994607738912 24.953018719257592 27.715828912739347 20.933157760264145 14-15 23.061822303309402 25.644403662643988 27.045587240060687 24.248186793985923 16-17 21.20777709371267 29.34969229576339 27.38562346063019 22.05690714989375 18-19 21.001317071113977 26.283858482388194 31.69871444426102 21.016110002236804 20-21 22.1719238742901 25.344793209830225 31.931006340950617 20.552276574929053 22-23 21.58463736237036 25.622392924548194 31.85096729332953 20.94200241975192 24-25 21.303357280730527 25.686138308903555 32.08722541068249 20.92327899968342 26-27 21.12280491125313 26.05910597813076 31.898847795031433 20.919241315584674 28-29 20.928424367030487 25.912927521069207 32.15014896553104 21.008499146369264 30-31 21.053985622986072 25.677312574634627 32.193455807368885 21.075245995010423 32-33 20.894443503509212 25.738914055928714 32.01604782904803 21.350594611514044 34-35 21.442996833455176 25.743773569534277 31.826276676407133 20.986952920603407 36-37 21.716344473768274 25.369448095034937 31.5114087801406 21.402798651056195 38-39 21.334979850884395 25.565508029988916 31.636719914072366 21.462792205054324 40-41 21.575668701230672 25.554324002352573 31.496758775888523 21.37324852052823 42-43 21.61279395590849 25.602168772336793 31.059616941693697 21.725420330061024 44-45 21.544939424019002 25.91778703467477 30.815712236755505 21.721561304550722 46-47 21.97018259622373 26.062000247263484 29.94535905731153 22.022458099201252 48-49 22.110822637043643 26.395234246421644 29.29182594079826 22.20211717573645 50-51 22.12725922718012 26.6910214054428 28.751419442485155 22.43029992489193 52-53 22.374951494193237 26.66861761845243 28.676204176751945 22.280226710602385 54-55 21.93534417147794 26.719785438181624 28.736626511362328 22.6082438789781 56-57 22.124793738659644 26.983378319565904 28.376986772832748 22.514841168941704 58-59 22.11653971187372 26.470914024484806 28.81852360830319 22.594022655338282 60-61 22.082201531175567 26.624846264284646 28.94976620737117 22.343185997168618 62-63 22.030211881939547 26.540769532564816 28.979423533052195 22.449595052443442 64-65 21.827505847495598 26.453191092511563 28.954983038153614 22.764320021839225 66-67 21.958355397669006 26.731112392688715 28.644117094268136 22.666415115374143 68-69 22.00223394698985 26.50024976470664 28.819452632963085 22.678063655340427 70-71 21.889071597072 26.45940841138927 28.8214893408713 22.83003065066743 72-73 22.06258481816486 26.5167220865608 28.586910614249668 22.833782481024674 74-75 21.744322408717967 26.702634213691397 28.45466752708643 23.098375850504212 76-77 21.769406074534935 26.634958340390348 28.53349169630613 23.062143888768592 78-79 21.887213547752225 26.639782122278227 28.395745924618936 23.07725840535061 80-81 21.465257693574795 26.970229047456723 28.299699067473632 23.26481419149485 82-83 21.691689588563566 26.74833508061433 28.266397106588432 23.29357822423368 84-85 21.65467152903881 26.409241079755336 28.217873433968144 23.71821395723771 86-87 21.508064291365 26.31991178553537 28.207725626144754 23.964298296954873 88-89 21.59256980369709 26.340064474311397 28.26300259340807 23.804363128583443 90-91 21.403155968233072 26.396484856540724 28.383204091710457 23.817155083515743 92-93 21.386969500120415 26.172625645225494 28.371627015179545 24.068777839474546 94-95 21.120385162771935 26.078705528353552 28.429301842451014 24.371607466423498 96-97 21.272754755168965 25.958653998365545 28.359099096974393 24.4094921494911 98-99 21.129388660518778 26.019947287477628 28.03465047938666 24.816013572616928 100-101 20.94240500946564 26.010829925010952 28.334825777136807 24.711939288386596 102-103 21.15387157947321 25.606528333480433 28.35434869067779 24.885251396368567 104-105 20.803870620527885 25.562383880848127 28.42993505725418 25.20381044136981 106-107 20.76366498006889 25.68143529732987 28.293958339438586 25.26094138316266 108-109 20.893637705556685 25.63102909692661 27.973508608272663 25.501824589244038 110-111 20.629519703091134 25.85337176328058 28.06374892116798 25.453359612460307 112-113 20.520318706018966 25.73730163523937 28.099540411710187 25.642839247031475 114-115 20.2150937326876 25.845667208749575 28.10969137015923 25.829547688403597 116-117 20.12199960112628 25.82214092251342 28.191400768725437 25.864458707634856 118-119 19.896668438058065 25.870414916568897 27.981146296320787 26.251770349052254 120-121 19.925589308019877 26.054750114814766 28.060014617662354 25.959645959502996 122-123 19.90591074099937 26.02507188565196 27.826948381706202 26.242068991642466 124-125 19.71917057005351 25.90383641548118 27.814700178078066 26.562292836387247 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 836.0 1 536.5 2 330.5 3 434.5 4 322.5 5 258.5 6 603.5 7 1325.5 8 2181.5 9 2613.5 10 2117.5 11 1449.0 12 1367.5 13 1512.0 14 1691.5 15 1918.5 16 2181.0 17 2422.5 18 2672.0 19 2973.5 20 3318.0 21 3740.5 22 4250.0 23 4928.5 24 5816.0 25 7027.5 26 8738.5 27 10746.5 28 12703.0 29 15243.0 30 18168.5 31 21288.5 32 24817.0 33 27414.0 34 29851.5 35 31986.5 36 34802.5 37 38489.5 38 40174.0 39 41115.5 40 42330.0 41 44408.0 42 45916.0 43 46650.5 44 47943.0 45 49764.0 46 49843.5 47 49071.0 48 50157.0 49 49993.5 50 48012.0 51 45672.0 52 44509.5 53 44492.5 54 43015.0 55 39931.5 56 36504.5 57 33347.5 58 31070.5 59 28079.0 60 24243.0 61 20305.0 62 17004.5 63 13301.0 64 9840.0 65 7719.5 66 5514.0 67 4294.5 68 3218.0 69 1900.5 70 1428.5 71 1256.0 72 1056.5 73 1053.0 74 877.5 75 670.5 76 468.5 77 222.5 78 122.0 79 70.0 80 41.5 81 24.0 82 14.0 83 6.5 84 4.0 85 1.5 86 1.0 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.17186956207921436 2 0.0018580493197752903 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 2.143903061279181E-4 12-13 0.006395977466149557 14-15 0.0 16-17 1.7865858843993176E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 8.932929421996587E-4 96-97 0.005466952806261912 98-99 0.0020724396259032083 100-101 0.0032873180272947446 102-103 0.004466464710998294 104-105 0.011541344813219591 106-107 0.01772293197324123 108-109 0.026584397959861845 110-111 0.0167581755956656 112-113 0.024833543793150517 114-115 0.0280136666673813 116-117 0.027013178572117682 118-119 0.016329394983409764 120-121 0.023189884779503143 122-123 0.015721955782713994 124-125 0.015114516582018228 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1399317.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.15506260102174 #Duplication Level Percentage of deduplicated Percentage of total 1 80.76114062760165 50.197137514385005 2 11.231814006163516 13.962282053522523 3 3.588992054669329 6.692220774976251 4 1.5316239546427879 3.807927311281879 5 0.8435125738831406 2.621428841722779 6 0.5018791223099331 1.8716596959191842 7 0.3097569876349344 1.3477075470287285 8 0.22053079068399786 1.0965684080333369 9 0.16822661997111296 0.9410522485916537 >10 0.7669616668052529 8.464643103315748 >50 0.04567414623692312 1.9529305507508552 >100 0.02630937447527301 2.7468457089420646 >500 0.0025256999450793236 1.035708789510002 >1k 6.314249862698309E-4 1.1075294444854966 >5k 4.209499908465539E-4 2.1543580075345097 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8399 0.6002213937227947 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8069 0.5766384600487238 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4452 0.31815521429383053 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2567 0.18344663861012195 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1447 0.10340759098903252 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 3.573171768798635E-5 0.0 20-21 0.0 0.0 0.0 1.7865858843993176E-4 0.0 22-23 0.0 0.0 0.0 5.002440476318089E-4 0.0 24-25 0.0 0.0 0.0 9.290246598876452E-4 0.0 26-27 0.0 0.0 0.0 0.0019652444728392496 0.0 28-29 0.0 0.0 0.0 0.004716586734814199 0.0 30-31 0.0 0.0 0.0 0.013899638180626691 0.0 32-33 0.0 0.0 0.0 0.03190842389537181 0.0 34-35 0.0 0.0 0.0 0.05717074830077816 0.0 36-37 0.0 0.0 0.0 0.10112076105700138 0.0 38-39 0.0 0.0 0.0 0.17136931803158256 0.0 40-41 0.0 0.0 0.0 0.24704909609473766 0.0 42-43 0.0 0.0 0.0 0.3206921662496775 0.0 44-45 0.0 0.0 0.0 0.4143807300275777 0.0 46-47 0.0 0.0 0.0 0.5295083244182698 0.0 48-49 0.0 0.0 0.0 0.654890921785414 0.0 50-51 0.0 0.0 0.0 0.7941016938978087 0.0 52-53 0.0 0.0 0.0 0.932276246197252 0.0 54-55 0.0 0.0 0.0 1.0827425093813625 0.0 56-57 0.0 0.0 0.0 1.2676184166990039 0.0 58-59 0.0 0.0 0.0 1.4753983550546446 0.0 60-61 0.0 0.0 0.0 1.715229644176409 0.0 62-63 0.0 0.0 0.0 1.9539532500498455 0.0 64-65 0.0 0.0 0.0 2.1916049043926433 0.0 66-67 0.0 0.0 0.0 2.4675609600969617 0.0 68-69 0.0 0.0 0.0 2.790790078302486 0.0 70-71 0.0 0.0 0.0 3.1627215277167364 0.0 72-73 0.0 0.0 0.0 3.537761636569841 0.0 74-75 0.0 0.0 0.0 3.9093000370895226 0.0 76-77 0.0 0.0 0.0 4.310138446113354 0.0 78-79 0.0 0.0 0.0 4.763609675291589 0.0 80-81 0.0 0.0 0.0 5.274180189335226 0.0 82-83 0.0 0.0 0.0 5.817945469111002 0.0 84-85 0.0 0.0 0.0 6.342379889617577 0.0 86-87 0.0 0.0 0.0 6.894256269308527 0.0 88-89 0.0 0.0 0.0 7.508234374341196 0.0 90-91 0.0 0.0 0.0 8.176131641365037 0.0 92-93 0.0 0.0 0.0 8.887514408815157 0.0 94-95 0.0 0.0 0.0 9.579673512149142 0.0 96-97 0.0 0.0 0.0 10.268259443714326 0.0 98-99 0.0 0.0 0.0 11.01240819628433 0.0 100-101 0.0 0.0 0.0 11.839990509655781 0.0 102-103 0.0 0.0 0.0 12.725136620222582 0.0 104-105 0.0 0.0 0.0 13.580375283084534 0.0 106-107 0.0 0.0 0.0 14.427359919160562 0.0 108-109 0.0 0.0 0.0 15.29814187921679 0.0 110-111 0.0 0.0 0.0 16.25353654675817 0.0 112-113 0.0 0.0 0.0 17.27174757399503 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4290 0.0 81.28646 1 GTGGTAT 1360 0.0 56.980217 1 GTATCAA 8325 0.0 56.423702 1 TGGTATC 1470 0.0 52.213676 2 TATCAAC 11145 0.0 42.068596 2 ATCAACG 11145 0.0 42.06559 3 TCAACGC 11285 0.0 41.54373 4 CAACGCA 11405 0.0 41.106617 5 AACGCAG 11835 0.0 39.765327 6 GTACATG 9780 0.0 34.55921 1 ACGCAGA 13815 0.0 33.810078 7 CGCAGAG 13870 0.0 33.547306 8 TACATGG 10365 0.0 32.5481 2 GCAGAGT 14815 0.0 31.16646 9 ACATGGG 10270 0.0 30.703386 3 GAGTACT 9430 0.0 28.141212 12-13 CATGGGA 7745 0.0 28.115156 4 ATGGGAG 3145 0.0 27.240978 5 CAGAGTA 14755 0.0 26.696 10-11 GTATTGG 610 0.0 26.384783 1 >>END_MODULE