FastQCFastQC Report
Thu 26 May 2016
SRR1364121_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364121_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3052546
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN125860.4123115589412903No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA44250.042.900911
GGTATCA31000.040.1211781
ATCAACG46700.039.5059553
TCAACGC46900.039.470234
CAACGCA48950.037.576515
AACGCAG51750.035.4283646
TATCAAC55200.033.5293162
GTACATG91900.031.9242361
TACATGG92600.030.9127392
ACGCAGA63950.028.5778797
ACATGGG95600.028.5118453
CGCAGAG63600.028.0799478
CATGGGG59950.023.7296244
GTGGTAT22450.021.2061961
GCAGAGT77400.020.8430149
GAGTACA66500.020.31391
AGTACAT67200.019.1288072
TGGTATC22550.019.0015642
AGAGTAC65500.018.95024510-11
CAGAGTA71050.018.39164410-11
Produced by FastQC (version 0.11.5)