##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364121_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3052546 Sequences flagged as poor quality 0 Sequence length 125 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.06861354423488 31.0 31.0 34.0 28.0 34.0 2 31.128310269525834 33.0 31.0 34.0 28.0 34.0 3 31.205844891444716 33.0 31.0 34.0 28.0 34.0 4 34.68591333267378 37.0 35.0 37.0 32.0 37.0 5 34.72126447889729 37.0 35.0 37.0 32.0 37.0 6 34.79930261493193 37.0 35.0 37.0 32.0 37.0 7 34.72740034056817 37.0 35.0 37.0 32.0 37.0 8 34.7050101783888 37.0 35.0 37.0 32.0 37.0 9 36.271573630667646 39.0 37.0 39.0 32.0 39.0 10-11 36.1956080268733 39.0 37.0 39.0 32.0 39.0 12-13 36.1058432862273 39.0 37.0 39.0 32.0 39.0 14-15 37.38775992237299 40.0 38.0 41.0 31.5 41.0 16-17 37.38891273055344 40.0 38.0 41.0 31.5 41.0 18-19 37.38593128490119 40.0 38.0 41.0 31.0 41.0 20-21 37.39847556760816 40.0 38.0 41.0 31.0 41.0 22-23 37.349347561019556 40.0 38.0 41.0 31.0 41.0 24-25 37.34793054715637 40.0 38.0 41.0 31.0 41.0 26-27 37.30010964617732 40.0 38.0 41.0 31.0 41.0 28-29 37.24886193361213 40.0 37.0 41.0 31.0 41.0 30-31 37.19692577933306 40.0 37.0 41.0 31.0 41.0 32-33 37.10368426880381 40.0 37.0 41.0 30.0 41.0 34-35 37.07836524003242 40.0 37.0 41.0 30.0 41.0 36-37 37.02241522322677 40.0 37.0 41.0 30.0 41.0 38-39 36.94494464620681 40.0 37.0 41.0 30.0 41.0 40-41 36.87468018499967 40.0 37.0 41.0 30.0 41.0 42-43 36.775361616172205 40.0 37.0 41.0 30.0 41.0 44-45 36.68671381201135 40.0 36.5 41.0 30.0 41.0 46-47 36.58544506782208 40.0 36.0 41.0 30.0 41.0 48-49 36.485916674146765 40.0 36.0 41.0 30.0 41.0 50-51 36.36863293788202 40.0 36.0 41.0 29.5 41.0 52-53 36.232706894507075 40.0 35.5 41.0 29.0 41.0 54-55 36.06457740522174 39.0 35.0 41.0 29.0 41.0 56-57 35.88309938654487 39.0 35.0 41.0 28.0 41.0 58-59 35.67639848834383 39.0 35.0 41.0 28.0 41.0 60-61 35.44145821225953 39.0 35.0 41.0 28.0 41.0 62-63 35.18632266311466 38.0 34.5 40.0 28.0 41.0 64-65 34.906929821860174 37.5 34.0 40.0 28.0 41.0 66-67 34.5973307199957 37.0 34.0 40.0 27.0 41.0 68-69 34.272251425531344 36.5 34.0 39.5 26.0 41.0 70-71 33.92547630731855 36.0 34.0 39.0 26.0 41.0 72-73 33.56810904733295 35.5 33.5 39.0 26.0 40.5 74-75 33.283524146728666 35.0 33.0 38.0 26.0 40.0 76-77 33.04908017766154 35.0 33.0 37.0 26.0 39.0 78-79 32.73969237482416 35.0 33.0 37.0 26.0 39.0 80-81 32.52267304079939 35.0 33.0 36.0 26.0 39.0 82-83 32.416479718896944 35.0 33.0 36.0 26.0 37.0 84-85 32.19906137368609 35.0 33.0 36.0 26.0 37.0 86-87 32.00131775245975 35.0 33.0 35.0 26.0 37.0 88-89 31.8221625489018 35.0 33.0 35.0 25.5 36.0 90-91 31.67742648268036 35.0 33.0 35.0 25.0 36.0 92-93 31.55593118007067 35.0 33.0 35.0 25.0 36.0 94-95 31.455813606084888 35.0 33.0 35.0 25.0 36.0 96-97 31.366617079644335 35.0 33.0 35.0 24.5 35.0 98-99 31.28661091429908 35.0 33.0 35.0 24.0 35.0 100-101 31.210090527710314 35.0 33.0 35.0 24.0 35.0 102-103 30.11215129927608 34.0 30.5 35.0 19.5 35.0 104-105 30.933243266440538 35.0 32.5 35.0 23.0 35.0 106-107 31.023886454127144 35.0 33.0 35.0 23.0 35.0 108-109 30.996671467031128 35.0 33.0 35.0 21.5 35.0 110-111 30.919337333491452 35.0 33.0 35.0 20.0 35.0 112-113 30.83300333557627 35.0 33.0 35.0 19.0 35.0 114-115 30.723237749734157 35.0 33.0 35.0 18.5 35.0 116-117 30.611368018696524 35.0 33.0 35.0 16.5 35.0 118-119 30.46717101069075 35.0 32.0 35.0 7.5 35.0 120-121 30.31356480786858 35.0 32.0 35.0 2.0 35.0 122-123 30.13502957858784 35.0 32.0 35.0 2.0 35.0 124-125 29.265909997752694 34.5 30.5 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.36036539215413654 1101 2 0.40550587866476207 1101 3 0.4898601633125601 1101 4 0.4245573298906251 1101 5 0.4710454868249272 1101 6 0.4926153650357179 1101 7 0.490868651947892 1101 8 0.5286343300344925 1101 9 0.5759085029734408 1101 10-11 0.5912051145036514 1101 12-13 0.6513243951332299 1101 14-15 0.7522013220380543 1101 16-17 0.7043489883755356 1101 18-19 0.7129761686512168 1101 20-21 0.6191751027088586 1101 22-23 0.7134777826543512 1101 24-25 0.6593973243270383 1101 26-27 0.6299917695680364 1101 28-29 0.7358363226510249 1101 30-31 0.7312218969973827 1101 32-33 0.7196497033567439 1101 34-35 0.6440003469199738 1101 36-37 0.6359433435069519 1101 38-39 0.6013340838644794 1101 40-41 0.6994992137174947 1101 42-43 0.7536503072736593 1101 44-45 0.7398773363596547 1101 46-47 0.7926447073435483 1101 48-49 0.5923311109711591 1101 50-51 0.714817657193052 1101 52-53 0.7677484041172491 1101 54-55 0.8055904888199521 1101 56-57 0.782861928310659 1101 58-59 0.7918857674040183 1101 60-61 0.773603987536319 1101 62-63 0.7675460736840307 1101 64-65 0.826081399363666 1101 66-67 0.7955837281821303 1101 68-69 0.7876304346595759 1101 70-71 0.779082827515289 1101 72-73 0.7809191609861514 1101 74-75 0.800587130229971 1101 76-77 0.7230936087613244 1101 78-79 0.6851011967926794 1101 80-81 0.680982453255254 1101 82-83 0.697805966208108 1101 84-85 0.6668062094424272 1101 86-87 0.7239713102150986 1101 88-89 0.7125053867282354 1101 90-91 0.7127734228272011 1101 92-93 0.6998003800494175 1101 94-95 0.675453266192573 1101 96-97 0.6740682051960682 1101 98-99 0.6461389119400565 1101 100-101 0.6156758790748142 1101 102-103 0.7092410212111204 1101 104-105 0.7074611111671238 1101 106-107 0.7093117783317666 1101 108-109 0.7327970260789627 1101 110-111 0.712058076926958 1101 112-113 0.7049943997084611 1101 114-115 0.7132561759697253 1101 116-117 0.7518025014870844 1101 118-119 0.7566099672115456 1101 120-121 0.7812217662044105 1101 122-123 0.829194260781037 1101 124-125 0.8450458467731572 1102 1 0.4448869835427125 1102 2 0.5169148321425361 1102 3 0.46195572097986215 1102 4 0.49759776298303393 1102 5 0.5320893211918545 1102 6 0.590867062963035 1102 7 0.5269280534086889 1102 8 0.5827980308235112 1102 9 0.6793193224951182 1102 10-11 0.6690849214837371 1102 12-13 0.7369739233428945 1102 14-15 0.7784970893944134 1102 16-17 0.8628340481808792 1102 18-19 0.7668644091621175 1102 20-21 0.7345887688708714 1102 22-23 0.7788337321346717 1102 24-25 0.8081231913168097 1102 26-27 0.8400764260541678 1102 28-29 0.8694370918320686 1102 30-31 0.8881473367401824 1102 32-33 0.8515580905576456 1102 34-35 0.8592327081279407 1102 36-37 0.9145889541485417 1102 38-39 0.8846147440136392 1102 40-41 0.8656878270758455 1102 42-43 0.834473509449964 1102 44-45 0.8656375997014933 1102 46-47 0.8558175500614951 1102 48-49 0.8780913652798716 1102 50-51 0.8670728934977205 1102 52-53 0.951733287073985 1102 54-55 0.811532230752654 1102 56-57 0.8888362497319022 1102 58-59 0.9086147074645794 1102 60-61 0.9500346395958132 1102 62-63 0.9603469903259025 1102 64-65 0.9458224867758602 1102 66-67 0.9463264170678869 1102 68-69 0.9240104971768659 1102 70-71 0.9145516730531824 1102 72-73 0.9410346254636792 1102 74-75 0.9198848164238527 1102 76-77 0.9566105555152404 1102 78-79 0.7935658015771878 1102 80-81 0.8786493162419475 1102 82-83 0.7759011631524189 1102 84-85 0.8029225547504737 1102 86-87 0.8031731408491325 1102 88-89 0.8058153603876228 1102 90-91 0.8108885197566984 1102 92-93 0.8018746476332055 1102 94-95 0.8004991093268465 1102 96-97 0.872651303567654 1102 98-99 0.8622749876111868 1102 100-101 0.8643140594652152 1102 102-103 0.9243300316658605 1102 104-105 0.9457504611872594 1102 106-107 0.992740330891138 1102 108-109 0.9844672016147555 1102 110-111 0.9997044487602977 1102 112-113 0.9858392512861052 1102 114-115 1.0473196371198377 1102 116-117 1.0604435985747642 1102 118-119 1.0360184507658303 1102 120-121 0.9852069773648644 1102 122-123 0.9754185780871865 1102 124-125 1.1575055740689884 1103 1 0.2565748034104942 1103 2 0.37537097306288914 1103 3 0.37512210091515996 1103 4 0.39245796791481524 1103 5 0.3910972847499181 1103 6 0.3609071512546578 1103 7 0.3983682563928568 1103 8 0.4506649117272019 1103 9 0.48475720998821714 1103 10-11 0.448925947873839 1103 12-13 0.4416081514945063 1103 14-15 0.5767687213828125 1103 16-17 0.5143255923104562 1103 18-19 0.5160231501408887 1103 20-21 0.5589164379071292 1103 22-23 0.6131638627326907 1103 24-25 0.6072381110861258 1103 26-27 0.6666999748147333 1103 28-29 0.708474621181459 1103 30-31 0.6116003398455376 1103 32-33 0.3399373550105267 1103 34-35 0.5963345302246523 1103 36-37 0.6393985212382773 1103 38-39 0.6761258724368346 1103 40-41 0.6463193934947356 1103 42-43 0.661013879062935 1103 44-45 0.6285574177082154 1103 46-47 0.5806298221641626 1103 48-49 0.592306749229401 1103 50-51 0.5978679779482263 1103 52-53 0.5046135848418061 1103 54-55 0.5455361367913341 1103 56-57 0.5722591189932231 1103 58-59 0.5886799794265869 1103 60-61 0.6336799961706134 1103 62-63 0.6445691909080224 1103 64-65 0.6163796382890467 1103 66-67 0.6357943139744364 1103 68-69 0.6248137507664211 1103 70-71 0.5862442537437218 1103 72-73 0.5991784726412632 1103 74-75 0.6154775388570641 1103 76-77 0.5935193749393974 1103 78-79 0.5558059850852644 1103 80-81 0.5266595087306669 1103 82-83 0.5200251988153042 1103 84-85 0.5432041195463526 1103 86-87 0.4740528651542135 1103 88-89 0.4719180318583902 1103 90-91 0.4880087088912042 1103 92-93 0.5081291785298632 1103 94-95 0.5173958373432122 1103 96-97 0.501287517198751 1103 98-99 0.5437617438678863 1103 100-101 0.6277870526161209 1103 102-103 0.5512749359625033 1103 104-105 0.5122234489281396 1103 106-107 0.5537830051751165 1103 108-109 0.5748944313569169 1103 110-111 0.5322890345146156 1103 112-113 0.5440436907533268 1103 114-115 0.5877014226447699 1103 116-117 0.5827444014226018 1103 118-119 0.6182461100640424 1103 120-121 0.5874287397348894 1103 122-123 0.5952919023316774 1103 124-125 0.6466517096875144 1104 1 0.3350532958274073 1104 2 0.38739852233027605 1104 3 0.38365790825837465 1104 4 0.3742625341182091 1104 5 0.3356689912045425 1104 6 0.30382384484504854 1104 7 0.36838538746726357 1104 8 0.4718785332217692 1104 9 0.4392028709300675 1104 10-11 0.5514373172707039 1104 12-13 0.530369232314527 1104 14-15 0.5897773560874455 1104 16-17 0.6263953397795561 1104 18-19 0.6654040257702647 1104 20-21 0.5881744139284848 1104 22-23 0.5798791589589172 1104 24-25 0.6001009723911608 1104 26-27 0.6446038618121293 1104 28-29 0.5976992603200983 1104 30-31 0.6415634141438034 1104 32-33 -0.029196495804427514 1104 34-35 0.5408071118596709 1104 36-37 0.646591706789927 1104 38-39 0.6847515519386107 1104 40-41 0.7219323671210063 1104 42-43 0.7667237113652732 1104 44-45 0.7021655217044938 1104 46-47 0.6656607154342638 1104 48-49 0.7009251024438186 1104 50-51 0.6500438094515033 1104 52-53 0.6228832977878582 1104 54-55 0.6635839375551456 1104 56-57 0.6295419643472755 1104 58-59 0.672861068868265 1104 60-61 0.7618979882582266 1104 62-63 0.763586700886492 1104 64-65 0.7097556734115429 1104 66-67 0.7547221793187973 1104 68-69 0.7355977575569597 1104 70-71 0.7541485017465845 1104 72-73 0.6604969950428696 1104 74-75 0.7106366831102946 1104 76-77 0.7114565229780254 1104 78-79 0.7452132656857131 1104 80-81 0.6378220403679435 1104 82-83 0.6803735872899495 1104 84-85 0.6946557710553378 1104 86-87 0.7097996716968495 1104 88-89 0.6948340519764109 1104 90-91 0.7225464504956314 1104 92-93 0.6860865186052081 1104 94-95 0.6540167624940345 1104 96-97 0.6647310724776396 1104 98-99 0.6855733360263656 1104 100-101 0.724068423308367 1104 102-103 0.721032218944373 1104 104-105 0.762094063356404 1104 106-107 0.7745461130863234 1104 108-109 0.740213546535557 1104 110-111 0.7782569220168192 1104 112-113 0.7175319196999581 1104 114-115 0.7792688203930069 1104 116-117 0.7874056797035109 1104 118-119 0.8241897161567202 1104 120-121 0.8134955184649328 1104 122-123 0.8707821492685142 1104 124-125 0.9059912092368663 1105 1 0.27288886047425365 1105 2 0.3319096544003557 1105 3 0.32933675426303566 1105 4 0.3519596062592498 1105 5 0.33281299156384136 1105 6 0.3455573692588487 1105 7 0.2753969788653805 1105 8 0.3150986472984343 1105 9 0.321539360357356 1105 10-11 0.4408648108094937 1105 12-13 0.5114347100360987 1105 14-15 0.5830201303471156 1105 16-17 0.5304958121729157 1105 18-19 0.5399013167555964 1105 20-21 0.5581986664486607 1105 22-23 0.5110534463880256 1105 24-25 0.6181607299476184 1105 26-27 0.6528666627740733 1105 28-29 0.5976349543953745 1105 30-31 0.6292238217004638 1105 32-33 0.32285802745390413 1105 34-35 0.6527024830579649 1105 36-37 0.6471969990654571 1105 38-39 0.6518024209872522 1105 40-41 0.681008280775508 1105 42-43 0.6113340582906375 1105 44-45 0.6247340432398332 1105 46-47 0.6273476537781519 1105 48-49 0.6110938085269595 1105 50-51 0.6520944539397746 1105 52-53 0.6593243425243642 1105 54-55 0.6513923508115553 1105 56-57 0.6207647159875904 1105 58-59 0.6055297035003804 1105 60-61 0.6312242663129624 1105 62-63 0.6880098869490467 1105 64-65 0.6322323293401411 1105 66-67 0.6453367806424737 1105 68-69 0.6683807138330167 1105 70-71 0.626620666467737 1105 72-73 0.6005444793542623 1105 74-75 0.624529008453365 1105 76-77 0.5499312681020072 1105 78-79 0.5282307854776462 1105 80-81 0.567657131396551 1105 82-83 0.5526160879942523 1105 84-85 0.5558825647823511 1105 86-87 0.5234921383088036 1105 88-89 0.5663548973033095 1105 90-91 0.5874305160999178 1105 92-93 0.6182468302133373 1105 94-95 0.6093221033562664 1105 96-97 0.6289626881282722 1105 98-99 0.5987531925526994 1105 100-101 0.615463882289351 1105 102-103 0.6385328211744614 1105 104-105 0.6407814467381208 1105 106-107 0.6794080492866783 1105 108-109 0.6468035768716263 1105 110-111 0.6136592729597652 1105 112-113 0.6333432154949605 1105 114-115 0.707787171058321 1105 116-117 0.6381046748979173 1105 118-119 0.6799586721143065 1105 120-121 0.7530469284999199 1105 122-123 0.7533207430306348 1105 124-125 0.8438982249111788 1106 1 0.4234601906997426 1106 2 0.4464956080418965 1106 3 0.4721914811071848 1106 4 0.5048895336395844 1106 5 0.45250980751453085 1106 6 0.4286045471709201 1106 7 0.5112399243774632 1106 8 0.4654359085004245 1106 9 0.5626740849162459 1106 10-11 0.5675065271678577 1106 12-13 0.54916274258008 1106 14-15 0.6809533591381438 1106 16-17 0.6518151940260424 1106 18-19 0.7238937810689521 1106 20-21 0.7381230622961823 1106 22-23 0.6887006078712048 1106 24-25 0.6998308515128642 1106 26-27 0.7091370976251454 1106 28-29 0.684347792491522 1106 30-31 0.7437442587543686 1106 32-33 0.747006517886355 1106 34-35 0.7719432410266123 1106 36-37 0.7991660638666218 1106 38-39 0.8110659264288174 1106 40-41 0.8515357362050366 1106 42-43 0.8370634312954763 1106 44-45 0.7945744272096675 1106 46-47 0.7823460435765455 1106 48-49 0.8794333368018457 1106 50-51 0.7913567115816917 1106 52-53 0.8368960391311191 1106 54-55 0.8055138700119002 1106 56-57 0.8978552565008542 1106 58-59 0.8206406283416214 1106 60-61 0.8677648000471834 1106 62-63 0.8772772892774583 1106 64-65 0.8616852757816744 1106 66-67 0.8481254707456074 1106 68-69 0.8656434137474349 1106 70-71 0.7846185363969909 1106 72-73 0.7638868542609245 1106 74-75 0.8098455900053168 1106 76-77 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>>END_MODULE >>Per sequence quality scores pass #Quality Count 2 56441.0 3 9335.0 4 5175.0 5 4372.0 6 4687.0 7 5688.0 8 6521.0 9 7441.0 10 8332.0 11 8867.0 12 9569.0 13 9595.0 14 9436.0 15 8781.0 16 7804.0 17 7299.0 18 7063.0 19 7270.0 20 7789.0 21 8629.0 22 10076.0 23 13112.0 24 17099.0 25 21683.0 26 26927.0 27 32384.0 28 39095.0 29 48607.0 30 58233.0 31 71490.0 32 91697.0 33 121269.0 34 167763.0 35 248662.0 36 472155.0 37 953796.0 38 456640.0 39 1764.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.25096603887854 18.21363447853649 12.824026226227545 24.711373256357426 2 15.879511497875054 19.73182869711374 39.997551367794756 24.391108437216445 3 19.74296512576944 23.577524471989513 28.70275418485501 27.97675621738604 4 12.879862413308999 15.935841583115748 36.98547323068742 34.19882277288784 5 14.209490985673245 37.09036969946101 34.58517048710178 14.114968827763974 6 33.35207096986863 35.331542555346005 17.56296125787829 13.753425216907079 7 29.001579619646556 31.32115409375703 21.093602589825434 18.583663696770984 8 25.722109520108543 35.322935465598775 20.090695928233636 18.86425908605904 9 25.798955397742894 16.54524430644345 19.98352638725637 37.67227390855729 10-11 24.940682909925314 25.999101390966413 28.152666691481627 20.907549007626645 12-13 25.436591025588655 23.42042533117404 28.02755929375892 23.115424349478385 14-15 22.504704515890616 25.52256067948574 25.41802137127313 26.554713433350507 16-17 22.817347825807737 28.45717129325524 26.061259450190775 22.664221430746245 18-19 22.553695156961663 27.766492930895236 27.27296981450764 22.40684209763546 20-21 23.277254542615907 27.147428908711586 27.23530694401168 22.34000960466083 22-23 22.987666887371404 26.97249096592912 27.00556373590537 23.03427841079411 24-25 22.782502317253208 27.1978496153722 27.119257345873837 22.90039072150075 26-27 22.825813220541935 27.436836899653045 26.917861410534826 22.81948846927019 28-29 22.707200443698067 27.487345613854234 27.038624983903098 22.766828958544597 30-31 22.932992284910252 27.283270007061628 26.965480375003953 22.818257333024167 32-33 22.642431676256063 27.23755331095688 27.100386238366365 23.01962877442069 34-35 23.1067510923057 27.356347658453288 26.807605158354065 22.72929609088695 36-37 23.152476896754067 27.157763752284815 26.794300664395777 22.895458686565345 38-39 22.764946946603697 27.32762459065972 26.976310176846845 22.931118285889735 40-41 23.12639653544033 27.15679370271524 26.940291631153396 22.776518130691038 42-43 22.88355770113702 27.199677197356888 26.83227855287786 23.084486548628234 44-45 22.869919955527006 27.321497895909435 26.888929592164697 22.91965255639886 46-47 22.923915878829572 27.31866032172883 26.69016257600307 23.067261223438525 48-49 22.913066386276185 27.24686712852308 26.890327236168133 22.949739249032604 50-51 22.932996032568877 27.34145580431816 26.816891895894923 22.90865626721804 52-53 22.984518430248183 27.215376212200184 26.79055586887807 23.00954948867357 54-55 22.86291534826074 27.08032237092073 26.883534396921956 23.173227883896576 56-57 22.831525774474766 27.40607365442449 26.8589607223457 22.903439848755045 58-59 22.907921320932125 27.211413052600264 26.847140434441624 23.033525192025987 60-61 23.00241704440013 27.138378383537177 26.89838524189449 22.9608193301682 62-63 22.73676938098709 27.19199486924862 27.014403330446164 23.056832419318127 64-65 22.71537552330417 27.078446171301124 26.953731995193124 23.25244631020158 66-67 22.713492410950877 27.234942872284744 26.768720806601863 23.282843910162512 68-69 22.636630067379887 27.077595919842047 27.015738434024577 23.270035578753482 70-71 22.659154731135132 26.860743838312878 27.05919706519 23.420904365361984 72-73 22.641472783995805 26.875252682883144 27.015899103313224 23.467375429807824 74-75 22.559192841867066 27.102255491331572 26.84996096578371 23.488590701017657 76-77 22.706382833040742 26.926804330386346 26.937552278976582 23.429260557596333 78-79 22.60046931084065 26.890902102351244 27.093145279771647 23.415483307036453 80-81 22.562883674152985 27.005277924318204 27.0471523651899 23.384686036338916 82-83 22.64993808425118 27.01240175000259 27.036236391580115 23.301423774166114 84-85 22.722503109678446 26.849845120977477 26.994560181927508 23.43309158741657 86-87 22.56634124070472 26.72033633018397 27.20271484186629 23.510607587245023 88-89 22.59980515706681 26.73920525216754 27.1361825038683 23.524807086897347 90-91 22.65884026884427 26.746683838750705 27.174773624876963 23.41970226752806 92-93 22.503033375939655 26.532613913490238 27.273773019386123 23.69057969118398 94-95 22.450667137518217 26.576827488471572 27.32348702873637 23.64901834527384 96-97 22.45512198950528 26.448700960965894 27.293665799483374 23.802511250045455 98-99 22.429924373572412 26.357797759218176 27.28781909396648 23.924458773242925 100-101 22.374635605915568 26.30380877756352 27.41246811654558 23.90908749997533 102-103 22.27378361963641 26.204868267911465 27.473895911906816 24.04745220054531 104-105 22.213829018097087 26.308435285271358 27.374481852587923 24.103253844043632 106-107 22.197020867675455 26.259657938073083 27.41049421243245 24.132826981819008 108-109 22.169216150346806 26.30789507922976 27.288716669871377 24.23417210055206 110-111 22.153773561468967 26.395376786899146 27.362637308399187 24.088212343232705 112-113 22.144370623623878 26.361403507040126 27.474276643977504 24.0199492253585 114-115 22.014056106967367 26.48706396283773 27.287703711307742 24.211176218887157 116-117 21.87777491547149 26.31814962097472 27.55570228987793 24.248373173675862 118-119 22.0044964130894 26.38087130223015 27.344665812761804 24.269966471918647 120-121 21.995999575438283 26.41028953299034 27.36603061945694 24.22768027211444 122-123 21.888199223682488 26.488314769838766 27.41729007415089 24.206195932327855 124-125 21.87139038220552 26.4395326451345 27.31836892697872 24.37070804568126 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 12572.0 1 6609.5 2 637.0 3 569.0 4 451.5 5 365.5 6 324.0 7 283.5 8 278.5 9 300.0 10 283.5 11 256.0 12 258.5 13 291.5 14 314.5 15 338.5 16 407.0 17 488.5 18 576.5 19 738.5 20 1003.5 21 1416.5 22 2012.5 23 2844.0 24 4175.5 25 6199.0 26 9294.0 27 13270.5 28 17258.5 29 22468.0 30 29015.0 31 35594.0 32 43138.5 33 50202.0 34 56666.5 35 63451.0 36 71442.0 37 79911.0 38 86222.0 39 91696.0 40 96196.0 41 100994.0 42 106305.5 43 110516.5 44 114512.0 45 118105.5 46 120781.0 47 122451.5 48 121851.5 49 120670.0 50 118525.5 51 113635.5 52 111519.5 53 112179.0 54 110603.5 55 102963.0 56 90841.0 57 80579.0 58 73499.0 59 64582.5 60 52765.0 61 42166.5 62 33791.5 63 26550.5 64 20552.0 65 15737.0 66 11416.5 67 8992.0 68 6685.0 69 4173.0 70 2891.0 71 2079.0 72 1666.0 73 1344.5 74 948.0 75 714.5 76 511.0 77 286.0 78 173.0 79 109.5 80 74.0 81 52.0 82 32.5 83 24.5 84 23.0 85 21.0 86 19.5 87 17.5 88 14.0 89 11.0 90 11.5 91 9.0 92 6.0 93 8.5 94 7.0 95 2.5 96 3.5 97 3.5 98 2.0 99 1.5 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.47062353851506256 2 0.4624008942043789 3 0.45765076103685254 4 0.4547024025190775 5 0.462073298813515 6 0.5201232020745961 7 0.5156023856806744 8 0.5250043733984681 9 0.52988554472234 10-11 0.5484929629234089 12-13 0.5563224927650559 14-15 0.5361917559964698 16-17 0.5295743291010193 18-19 0.5411220666289714 20-21 0.5411875857071442 22-23 0.5508844092767152 24-25 0.5445945777721286 26-27 0.5521128919924548 28-29 0.53224423153656 30-31 0.5378461127203324 32-33 0.5394677099051087 34-35 0.5379935306462212 36-37 0.4856110276470854 38-39 0.45257303247846226 40-41 0.4512954104540931 42-43 0.46377679484600726 44-45 0.4683795100876449 46-47 0.47267100970796183 48-49 0.4873800427577504 50-51 0.46872348524805196 52-53 0.4691329794866318 54-55 0.4585188888226418 56-57 0.4423356765139657 58-59 0.4594525356866039 60-61 0.4559308852348171 62-63 0.4715571853790246 64-65 0.4717046033049134 66-67 0.4788134232866597 68-69 0.4887231838602924 70-71 0.48492307732627127 72-73 0.49604494084610024 74-75 0.4857093062643446 76-77 0.48343251829784056 78-79 0.4951112939821382 80-81 0.4879041953831326 82-83 0.48977148911105683 84-85 0.4865610542805907 86-87 0.4776013203404633 88-89 0.48204023788666905 90-91 0.46785535746226264 92-93 0.4672984452977941 94-95 0.46123793056681206 96-97 0.4514428283799818 98-99 0.4150469804550038 100-101 0.41172188723773534 102-103 0.40181212666410265 104-105 0.40582517020218534 106-107 0.4050880805727416 108-109 0.4176022245037421 110-111 0.4405339018642144 112-113 0.43997698969974575 114-115 0.4332940437261224 116-117 0.4271516301474244 118-119 0.46998472750287795 120-121 0.46251555259118127 122-123 0.4281016567809297 124-125 0.43755278380735296 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 3052546.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 73.7393134177586 #Duplication Level Percentage of deduplicated Percentage of total 1 84.28598201921486 62.1519044483845 2 10.011753673364977 14.765196839633122 3 2.8522742063100717 6.309742249574611 4 1.1733682273214892 3.460934698755965 5 0.5418426549616041 1.9977552678662076 6 0.3124349593022241 1.3823243631990814 7 0.18593360885058294 0.959743166054965 8 0.12965222947983587 0.7648373107939823 9 0.08637443979033645 0.5732272698284646 >10 0.39288521288888173 4.9978710404069595 >50 0.020852516312783415 1.0555869261591817 >100 0.006459053891649127 0.6760324190279557 >500 9.359915538714049E-5 0.03616615226278437 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 9.359915538714049E-5 0.8686778480522979 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 12586 0.4123115589412903 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 6.551907817277773E-5 0.0 5 0.0 0.0 0.0 6.551907817277773E-5 0.0 6 0.0 0.0 0.0 6.551907817277773E-5 0.0 7 0.0 0.0 0.0 6.551907817277773E-5 0.0 8 0.0 0.0 0.0 6.551907817277773E-5 0.0 9 0.0 0.0 0.0 6.551907817277773E-5 0.0 10-11 0.0 0.0 0.0 6.551907817277773E-5 0.0 12-13 0.0 0.0 0.0 1.1465838680236104E-4 0.0 14-15 0.0 0.0 0.0 1.9655723451833323E-4 0.0 16-17 0.0 0.0 0.0 1.9655723451833323E-4 0.0 18-19 0.0 0.0 0.0 1.9655723451833323E-4 0.0 20-21 0.0 0.0 0.0 2.456965431479165E-4 0.0 22-23 0.0 0.0 0.0 4.5863354720944416E-4 0.0 24-25 0.0 0.0 0.0 7.043300903573607E-4 0.0 26-27 0.0 0.0 0.0 0.0011793434071099994 0.0 28-29 0.0 0.0 0.0 0.0022767879665040267 0.0 30-31 0.0 0.0 0.0 0.00753469398986944 0.0 32-33 0.0 0.0 0.0 0.02104800386300485 0.0 34-35 0.0 0.0 0.0 0.04253826150367594 0.0 36-37 0.0 0.0 0.0 0.07893410942865398 0.0 38-39 0.0 0.0 0.0 0.12740184750696631 0.0 40-41 0.0 0.0 0.0 0.18001366727970686 0.0 42-43 0.0 0.0 0.0 0.2322323725834107 0.0 44-45 0.0 0.0 0.0 0.3024360648455421 0.0 46-47 0.0 0.0 0.0 0.3901005914407187 0.0 48-49 0.0 0.0 0.0 0.48086089447955904 1.6379769543194433E-5 50-51 0.0 0.0 0.0 0.5766825463072465 3.275953908638887E-5 52-53 0.0 0.0 0.0 0.6692118644567518 3.275953908638887E-5 54-55 0.0 0.0 0.0 0.7812331083626586 3.275953908638887E-5 56-57 0.0 0.0 0.0 0.9182007412828505 3.275953908638887E-5 58-59 0.0 0.0 0.0 1.068370468454857 4.91393086295833E-5 60-61 0.0 0.0 0.0 1.23036638923705 6.551907817277773E-5 62-63 0.0 0.0 0.0 1.3966210501004737 6.551907817277773E-5 64-65 0.0 0.0 0.0 1.575848488442107 6.551907817277773E-5 66-67 0.0 0.0 0.0 1.7863285270721554 6.551907817277773E-5 68-69 0.0 0.0 0.0 2.029764662023111 6.551907817277773E-5 70-71 0.0 0.0 0.0 2.2997687831731284 6.551907817277773E-5 72-73 0.0 0.0 0.0 2.570673791648021 6.551907817277773E-5 74-75 0.0 0.0 0.0 2.8583025448265156 6.551907817277773E-5 76-77 0.0 0.0 0.0 3.1799848388853107 6.551907817277773E-5 78-79 0.0 0.0 0.0 3.5459088904802742 6.551907817277773E-5 80-81 0.0 0.0 0.0 3.94102169140121 6.551907817277773E-5 82-83 0.0 0.0 0.0 4.350990943297824 6.551907817277773E-5 84-85 0.0 0.0 3.275953908638887E-5 4.766824152690901 6.551907817277773E-5 86-87 0.0 3.275953908638887E-5 3.275953908638887E-5 5.205736457370339 6.551907817277773E-5 88-89 0.0 3.275953908638887E-5 3.275953908638887E-5 5.700667573887502 6.551907817277773E-5 90-91 0.0 3.275953908638887E-5 3.275953908638887E-5 6.233403198510358 6.551907817277773E-5 92-93 0.0 3.275953908638887E-5 3.275953908638887E-5 6.786171281284541 6.551907817277773E-5 94-95 0.0 3.275953908638887E-5 3.275953908638887E-5 7.346343019892247 6.551907817277773E-5 96-97 0.0 3.275953908638887E-5 3.275953908638887E-5 7.92341868066853 6.551907817277773E-5 98-99 0.0 3.275953908638887E-5 3.275953908638887E-5 8.550092283621606 6.551907817277773E-5 100-101 0.0 3.275953908638887E-5 3.275953908638887E-5 9.229066490726101 6.551907817277773E-5 102-103 0.0 3.275953908638887E-5 3.275953908638887E-5 9.929661993627615 6.551907817277773E-5 104-105 0.0 3.275953908638887E-5 3.275953908638887E-5 10.62242796668748 6.551907817277773E-5 106-107 0.0 3.275953908638887E-5 3.275953908638887E-5 11.321270834247871 6.551907817277773E-5 108-109 0.0 3.275953908638887E-5 3.275953908638887E-5 12.060850188662187 6.551907817277773E-5 110-111 0.0 3.275953908638887E-5 3.275953908638887E-5 12.840805675000475 6.551907817277773E-5 112-113 0.0 3.275953908638887E-5 3.275953908638887E-5 13.661022634875938 6.551907817277773E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 4425 0.0 42.90091 1 GGTATCA 3100 0.0 40.121178 1 ATCAACG 4670 0.0 39.505955 3 TCAACGC 4690 0.0 39.47023 4 CAACGCA 4895 0.0 37.57651 5 AACGCAG 5175 0.0 35.428364 6 TATCAAC 5520 0.0 33.529316 2 GTACATG 9190 0.0 31.924236 1 TACATGG 9260 0.0 30.912739 2 ACGCAGA 6395 0.0 28.577879 7 ACATGGG 9560 0.0 28.511845 3 CGCAGAG 6360 0.0 28.079947 8 CATGGGG 5995 0.0 23.729624 4 GTGGTAT 2245 0.0 21.206196 1 GCAGAGT 7740 0.0 20.843014 9 GAGTACA 6650 0.0 20.3139 1 AGTACAT 6720 0.0 19.128807 2 TGGTATC 2255 0.0 19.001564 2 AGAGTAC 6550 0.0 18.950245 10-11 CAGAGTA 7105 0.0 18.391644 10-11 >>END_MODULE