FastQCFastQC Report
Thu 26 May 2016
SRR1364121_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364121_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3052546
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43620.14289710949482826No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38380.12573111101356046No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA46400.057.284571
GTATCAA73800.050.1482771
ATCAACG86150.042.5394133
TCAACGC87250.041.93564
CAACGCA90400.040.5401655
TATCAAC93150.039.5981522
AACGCAG93850.039.1766626
CGCAGAG112650.032.3802878
ACGCAGA114700.032.0090837
GTACATG85300.028.7153171
GTGGTAT27100.028.588731
TGGTATC24950.028.3754332
TACATGG86600.027.6168582
GCAGAGT130150.027.569239
ACATGGG88450.025.6267323
GAGTACT84300.025.12851312-13
CAGAGTA125600.024.2091310-11
AGAGTAC116400.023.2598210-11
GTACTTT90500.023.23843814-15
GAGTACA67050.021.0654341
Produced by FastQC (version 0.11.5)