FastQCFastQC Report
Thu 26 May 2016
SRR1364120_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364120_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2027023
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN84440.4165714942553686No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20550.053.86081
GTATCAA32650.042.2840421
ATCAACG33800.039.4457243
TCAACGC34550.038.2467964
CAACGCA35100.037.4797635
AACGCAG36650.035.732256
TATCAAC40800.032.8215182
GTACATG65250.031.4637341
TACATGG65800.030.3008792
ACATGGG65450.029.7376563
ACGCAGA45300.028.781377
CGCAGAG47100.027.5550448
CATGGGG40300.024.8138624
GAGTACA48150.023.6052591
GCAGAGT58950.021.3090559
CAGAGTA51900.020.81997310-11
AGTACAT53400.019.9502372
AGAGTAC48650.019.2126710-11
TAGGGTG7350.017.817515
GTGGTAT14350.017.4168281
Produced by FastQC (version 0.11.5)