FastQCFastQC Report
Thu 26 May 2016
SRR1364120_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364120_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2027023
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53690.26487119287743655No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51660.2548565063149259No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28650.1413402807960245No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA33000.072.587351
GTATCAA60700.052.616861
ATCAACG76050.041.6184433
TCAACGC77050.041.2327234
CAACGCA80000.039.7122655
TATCAAC81400.039.1033062
AACGCAG83300.038.4247176
ACGCAGA97150.032.5857667
GTGGTAT16700.032.469361
TGGTATC16400.032.2871932
CGCAGAG98500.032.0183338
GTACATG63000.029.7933141
GCAGAGT109600.028.1783739
TACATGG64600.028.089982
ACATGGG63900.027.2797373
GAGTACT77450.025.62320912-13
CAGAGTA105100.025.5065210-11
AGAGTAC100700.025.05506310-11
GTACTTT83450.023.7049314-15
GTACCGT2052.26651E-623.2171826
Produced by FastQC (version 0.11.5)