FastQCFastQC Report
Thu 26 May 2016
SRR1364119_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364119_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2908488
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN117460.40385244841993506No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA28650.052.965161
GTATCAA42700.044.0386471
TCAACGC48400.037.145094
CAACGCA48650.036.9567645
ATCAACG49200.036.6551323
TACATGG94850.034.823142
AACGCAG51750.034.5128446
GTACATG98950.034.339591
TATCAAC53750.033.9916422
ACATGGG96400.032.846713
ACGCAGA61650.029.0677077
CATGGGG68200.028.281374
CGCAGAG63450.028.055438
TAAGGTG15350.024.0465725
GAGTACA70350.022.9234491
CAGAGTA72950.020.93428810-11
AGAGTAC66250.020.6249510-11
CATGGGA35000.020.4104714
GCAGAGT84450.020.3034659
GTAAGGT15900.019.4691094
Produced by FastQC (version 0.11.5)