FastQCFastQC Report
Thu 26 May 2016
SRR1364119_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364119_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2908488
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59500.2045736478885249No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58850.20233880971831414No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32590.11205134764179876No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA45800.072.9716951
GTATCAA79300.051.460551
TCAACGC105150.038.362084
ATCAACG105300.038.3074383
CAACGCA106600.037.840275
TATCAAC108050.037.3881762
AACGCAG111050.036.7531666
TGGTATC19350.034.4370772
GTGGTAT21450.033.0503961
ACGCAGA130550.031.267727
CGCAGAG132150.030.8441188
TACATGG89300.030.514292
GTACATG91100.030.4736581
ACATGGG90050.029.136453
GCAGAGT157250.025.4667429
CAGAGTA143000.025.1749310-11
GAGTACT108500.024.92581612-13
AGAGTAC136450.024.7698710-11
GTACTTT114250.023.58977514-15
GAGTACA66300.022.9130921
Produced by FastQC (version 0.11.5)