FastQCFastQC Report
Thu 26 May 2016
SRR1364118_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364118_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2424859
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN99820.4116528012556606No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA27950.057.060761
GTATCAA44800.044.4991151
ATCAACG49300.038.5115783
TCAACGC49550.038.3220374
CAACGCA51750.036.8109665
GTACATG107850.036.8035961
AACGCAG53950.035.309876
TACATGG111300.035.022632
ACATGGG108950.034.2521553
TATCAAC59050.033.3588182
CATGGGA74500.029.4031034
ACGCAGA65700.028.7254967
CGCAGAG65650.028.5668
CATGGGG40600.025.9506844
GAGTACA76000.025.5263421
GTACCGT1751.7449134E-523.8120946
AGTACAT76550.023.4781762
GCAGAGT77150.023.3819279
ATGGGAG35800.022.115985
CAGAGTA73550.021.57233410-11
Produced by FastQC (version 0.11.5)