FastQCFastQC Report
Thu 26 May 2016
SRR1364118_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364118_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2424859
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75170.30999740603474263No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73080.30137834818436865No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41420.17081405558013887No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA48700.069.729491
GTATCAA83350.050.6770441
ATCAACG106700.039.253513
TCAACGC107300.039.0348134
CAACGCA110150.038.0248375
TATCAAC112000.037.612
AACGCAG113950.037.0700536
TGGTATC22050.035.8865052
GTACATG99800.034.5635761
TACATGG104550.032.8352172
GTGGTAT25400.032.6025621
ACATGGG98450.032.571913
ACGCAGA132150.031.7010947
CGCAGAG134050.031.2517688
GCAGAGT148850.027.5847459
CATGGGA70200.025.5946394
GAGTACT99900.025.37644412-13
CAGAGTA144850.025.22478910-11
AGAGTAC140100.024.91194210-11
GAGTACA73950.024.8132231
Produced by FastQC (version 0.11.5)