FastQCFastQC Report
Thu 26 May 2016
SRR1364117_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364117_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2266545
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN93230.41133090232049224No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA30450.067.0409551
GTATCAA49550.048.4055021
TCAACGC61500.037.75024
TACATGG128900.037.584162
ATCAACG62200.037.513443
GTACATG132800.037.2422871
CAACGCA62850.037.1295855
TATCAAC65100.036.6603622
ACATGGG129000.035.8066063
AACGCAG65350.035.7091756
TCGCGAC350.002788911634.002327116-117
CATGGGG59000.030.6726464
CATGGGA69650.030.2560234
ACGCAGA77750.029.709137
CGCAGAG79950.028.5193028
GAGTACA88900.027.3813531
TGGTATC14950.024.68222
ATGGGAG33150.024.6023395
AGTACAT98050.024.5226332
GCAGAGT92850.023.4670129
Produced by FastQC (version 0.11.5)