FastQCFastQC Report
Thu 26 May 2016
SRR1364117_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364117_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2266545
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88720.39143277543574034No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87690.3868884138633912No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49030.21632043484687047No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24430.10778519729367828No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA54350.080.377821
GTATCAA97850.055.3649441
ATCAACG128400.041.841443
TCAACGC129550.041.4709324
TATCAAC131100.041.2066232
CAACGCA131200.040.994735
TGGTATC18000.040.9857642
AACGCAG136800.039.664526
GTGGTAT21850.034.9132541
ACGCAGA159250.034.001917
CGCAGAG160750.033.4625368
GTACATG122000.032.1438521
TACATGG121100.031.6883372
ACATGGG120900.030.6089173
GCAGAGT179350.029.5940789
GAGTACT118100.028.11357512-13
CAGAGTA172500.027.57820110-11
CATGGGA60300.026.2456784
AGAGTAC167450.025.81588710-11
GAGTACA88450.025.7393361
Produced by FastQC (version 0.11.5)