FastQCFastQC Report
Thu 26 May 2016
SRR1364116_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364116_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2626494
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN108610.41351703068805795No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA26200.050.8798941
GTATCAA41450.040.918511
TCAACGC41850.039.5426064
ATCAACG43050.038.7102473
CAACGCA43250.038.400255
AACGCAG46950.035.3753856
TATCAAC47900.034.6631662
CGCAGAG54050.030.069388
ACGCAGA55000.029.8747257
TACATGG70900.027.1955762
GTACATG74300.026.5117661
ACATGGG72200.025.4719583
GAGTACA57900.021.7899781
GCAGAGT71100.021.4352199
TGGTATC16900.021.1282752
CATGGGG46250.020.464514
CAGAGTA64700.020.15161510-11
AGAGTAC60500.019.23806610-11
GTGTAGC23300.019.1561
TAGACCG2501.2576713E-519.0487255
Produced by FastQC (version 0.11.5)