##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364116_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2626494 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.06466947954193 31.0 31.0 34.0 28.0 34.0 2 31.10775391072662 33.0 31.0 34.0 28.0 34.0 3 31.197495977527456 33.0 31.0 34.0 28.0 34.0 4 34.67627871984478 37.0 35.0 37.0 32.0 37.0 5 34.722131480216596 37.0 35.0 37.0 32.0 37.0 6 34.80392530879568 37.0 35.0 37.0 32.0 37.0 7 34.69519937985771 37.0 35.0 37.0 32.0 37.0 8 34.67222616918219 37.0 35.0 37.0 32.0 37.0 9 36.22210330577568 39.0 37.0 39.0 32.0 39.0 10-11 36.14147586097665 39.0 37.0 39.0 32.0 39.0 12-13 36.05556970623196 39.0 37.0 39.0 31.0 39.0 14-15 37.32032473708297 40.0 38.0 41.0 31.0 41.0 16-17 37.31917110794846 40.0 38.0 41.0 31.0 41.0 18-19 37.32092858388407 40.0 38.0 41.0 31.0 41.0 20-21 37.34077176646892 40.0 38.0 41.0 31.0 41.0 22-23 37.3011605965976 40.0 38.0 41.0 31.0 41.0 24-25 37.30429271873456 40.0 37.5 41.0 31.0 41.0 26-27 37.272200697964664 40.0 37.0 41.0 31.0 41.0 28-29 37.22800889703156 40.0 37.0 41.0 31.0 41.0 30-31 37.175929775586766 40.0 37.0 41.0 31.0 41.0 32-33 37.08295887978423 40.0 37.0 41.0 30.0 41.0 34-35 37.05433954922418 40.0 37.0 41.0 30.0 41.0 36-37 36.993820279048805 40.0 37.0 41.0 30.0 41.0 38-39 36.91110621231193 40.0 37.0 41.0 30.0 41.0 40-41 36.83911309144433 40.0 37.0 41.0 30.0 41.0 42-43 36.734980167478014 40.0 37.0 41.0 30.0 41.0 44-45 36.64123561675755 40.0 36.0 41.0 30.0 41.0 46-47 36.54387293479444 40.0 36.0 41.0 30.0 41.0 48-49 36.44121117352638 40.0 36.0 41.0 30.0 41.0 50-51 36.32920197038333 40.0 36.0 41.0 29.5 41.0 52-53 36.19065301500784 40.0 35.0 41.0 29.0 41.0 54-55 36.02743010263873 39.0 35.0 41.0 29.0 41.0 56-57 35.85182338128318 39.0 35.0 41.0 28.0 41.0 58-59 35.65829505035991 39.0 35.0 41.0 28.0 41.0 60-61 35.428894754756726 39.0 35.0 41.0 28.0 41.0 62-63 35.17768591894746 38.0 34.5 40.0 28.0 41.0 64-65 34.90956023505098 38.0 34.0 40.0 27.5 41.0 66-67 34.61184472532585 37.0 34.0 40.0 27.0 41.0 68-69 34.29547716461565 37.0 34.0 39.5 26.0 41.0 70-71 33.95361973033253 36.0 34.0 39.0 26.0 41.0 72-73 33.61008191909062 36.0 33.0 39.0 26.0 40.5 74-75 33.332521985582304 35.0 33.0 38.5 26.0 40.0 76-77 33.10078378248723 35.0 33.0 37.0 26.0 39.5 78-79 32.78456794494866 35.0 33.0 37.0 26.0 39.0 80-81 32.56589126036458 35.0 33.0 36.5 26.0 39.0 82-83 32.44492353685179 35.0 33.0 36.0 26.0 38.0 84-85 32.21604180325559 35.0 33.0 36.0 26.0 37.0 86-87 32.01401640361638 35.0 33.0 35.5 26.0 37.0 88-89 31.817220599019073 35.0 33.0 35.0 25.0 36.0 90-91 31.66698610390886 35.0 33.0 35.0 25.0 36.0 92-93 31.537533304854303 35.0 33.0 35.0 25.0 36.0 94-95 31.428016016788924 35.0 33.0 35.0 25.0 36.0 96-97 31.335332576430787 35.0 33.0 35.0 24.0 35.5 98-99 31.25223701253458 35.0 33.0 35.0 24.0 35.0 100-101 31.175829261365152 35.0 33.0 35.0 23.5 35.0 102-103 30.065104850801106 34.0 30.5 35.0 19.5 35.0 104-105 30.898594476134345 35.0 32.0 35.0 22.5 35.0 106-107 30.989893561531076 35.0 33.0 35.0 23.0 35.0 108-109 30.97279757730267 35.0 33.0 35.0 21.0 35.0 110-111 30.893680701345595 35.0 33.0 35.0 19.5 35.0 112-113 30.811489003972596 35.0 33.0 35.0 19.0 35.0 114-115 30.705173131939382 35.0 33.0 35.0 18.0 35.0 116-117 30.608041366171022 35.0 33.0 35.0 16.0 35.0 118-119 30.47224417798023 35.0 32.0 35.0 6.0 35.0 120-121 30.327452870632868 35.0 32.0 35.0 2.0 35.0 122-123 30.163612214610048 35.0 32.0 35.0 2.0 35.0 124-125 29.34179099590557 34.5 30.5 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.38533602857336646 1101 2 0.4188835748936697 1101 3 0.4682926678284929 1101 4 0.46623617951885876 1101 5 0.3505308322748206 1101 6 0.3974919790783673 1101 7 0.43014303618880234 1101 8 0.3913540090183574 1101 9 0.35989656637971024 1101 10-11 0.4382799671140276 1101 12-13 0.5792429876515328 1101 14-15 0.6240454750807274 1101 16-17 0.6286796181161378 1101 18-19 0.6085299822072088 1101 20-21 0.6020633985706922 1101 22-23 0.5733335655148792 1101 24-25 0.6546867996974228 1101 26-27 0.5849655398309324 1101 28-29 0.6065346308809225 1101 30-31 0.6334213591587954 1101 32-33 0.697114718975854 1101 34-35 0.6777267422960804 1101 36-37 0.6253343651536625 1101 38-39 0.6679867283555154 1101 40-41 0.6569230186133552 1101 42-43 0.6087910737333928 1101 44-45 0.7007513812783017 1101 46-47 0.7263765527080679 1101 48-49 0.579029770261009 1101 50-51 0.6258762907476978 1101 52-53 0.6635046841035859 1101 54-55 0.7243021391128863 1101 56-57 0.6923535434683643 1101 58-59 0.6705512466558332 1101 60-61 0.6939117074037853 1101 62-63 0.634302475994815 1101 64-65 0.6875113915479218 1101 66-67 0.7497715492966321 1101 68-69 0.7496829994666996 1101 70-71 0.7721266203025152 1101 72-73 0.7482420659802926 1101 74-75 0.6673763209921972 1101 76-77 0.5998583965426008 1101 78-79 0.5827680409255862 1101 80-81 0.500499334171586 1101 82-83 0.49165277018580866 1101 84-85 0.5226465629840717 1101 86-87 0.48606853402627337 1101 88-89 0.5416231705462238 1101 90-91 0.5372088622209255 1101 92-93 0.576211737915834 1101 94-95 0.5871063952595108 1101 96-97 0.5937642421211713 1101 98-99 0.5445988266845525 1101 100-101 0.5547874013475145 1101 102-103 0.6001871203967717 1101 104-105 0.5307094120765967 1101 106-107 0.5723787927927049 1101 108-109 0.601015481184227 1101 110-111 0.5202168643069243 1101 112-113 0.47501333168977666 1101 114-115 0.5576819700292504 1101 116-117 0.6007138195304584 1101 118-119 0.533896076346128 1101 120-121 0.5906212150530692 1101 122-123 0.627347090179935 1101 124-125 0.6629049349562628 1102 1 0.38552084851746926 1102 2 0.4002013881248665 1102 3 0.44416422407419276 1102 4 0.47677969054186065 1102 5 0.4097109785837034 1102 6 0.41238476034687466 1102 7 0.4350973057120129 1102 8 0.43016402127557996 1102 9 0.5566419945604224 1102 10-11 0.5010762809353508 1102 12-13 0.5282751853494858 1102 14-15 0.5825644882306662 1102 16-17 0.6233335626005285 1102 18-19 0.5757767364166142 1102 20-21 0.6624773741873753 1102 22-23 0.631774221466344 1102 24-25 0.583493918571385 1102 26-27 0.5960356788944239 1102 28-29 0.6309237037474276 1102 30-31 0.6477105099262488 1102 32-33 0.653347769835797 1102 34-35 0.7031876023735464 1102 36-37 0.6352470456268691 1102 38-39 0.5560560359047457 1102 40-41 0.6760198745123347 1102 42-43 0.5764414766661403 1102 44-45 0.60101578681018 1102 46-47 0.5603620203713149 1102 48-49 0.6110950829478483 1102 50-51 0.5964086002641835 1102 52-53 0.537756542375817 1102 54-55 0.5413515578460846 1102 56-57 0.6279118899244125 1102 58-59 0.6581339505975876 1102 60-61 0.5706206250468142 1102 62-63 0.5343112010685118 1102 64-65 0.5883347142104114 1102 66-67 0.605430998509334 1102 68-69 0.5243080283689423 1102 70-71 0.5234529999697131 1102 72-73 0.5158378652539 1102 74-75 0.46176413318031706 1102 76-77 0.5292548264080992 1102 78-79 0.5259846576213789 1102 80-81 0.5654558232187767 1102 82-83 0.46828150559482395 1102 84-85 0.5545598361726576 1102 86-87 0.5178992945508654 1102 88-89 0.5475257215106879 1102 90-91 0.5511870199149378 1102 92-93 0.4696776053292453 1102 94-95 0.512358625698603 1102 96-97 0.5546750696217622 1102 98-99 0.5635032817226282 1102 100-101 0.6243464194166286 1102 102-103 0.6712616549881467 1102 104-105 0.5898755547474828 1102 106-107 0.6137607363685014 1102 108-109 0.6031194312770864 1102 110-111 0.5320979508881543 1102 112-113 0.4859905343286677 1102 114-115 0.579950913161003 1102 116-117 0.5444094027619784 1102 118-119 0.5228659827737481 1102 120-121 0.5770856284747161 1102 122-123 0.6399733528061944 1102 124-125 0.6379986750984372 1103 1 0.3347931617324278 1103 2 0.41861227486580077 1103 3 0.4485019259316836 1103 4 0.38850400148857744 1103 5 0.3878590072136703 1103 6 0.3669120331988225 1103 7 0.3378739377545017 1103 8 0.43477289010481 1103 9 0.5515374061866893 1103 10-11 0.5549391843169929 1103 12-13 0.5741916150827109 1103 14-15 0.6774849109408407 1103 16-17 0.6254801452836958 1103 18-19 0.5986723716257671 1103 20-21 0.5931124472351854 1103 22-23 0.6301526568280664 1103 24-25 0.6295090489265149 1103 26-27 0.6556181621037211 1103 28-29 0.7147741071282141 1103 30-31 0.6347395858827198 1103 32-33 0.4105338504299496 1103 34-35 0.6986346985285294 1103 36-37 0.7271137403591439 1103 38-39 0.7412301427628165 1103 40-41 0.7238940051070415 1103 42-43 0.7278936329422621 1103 44-45 0.6623441628095392 1103 46-47 0.7205432942798069 1103 48-49 0.6260625647292741 1103 50-51 0.5648529724897813 1103 52-53 0.5632727656758263 1103 54-55 0.5510525476020689 1103 56-57 0.6251780558568427 1103 58-59 0.5800438540379105 1103 60-61 0.6399668161967327 1103 62-63 0.6786545922376419 1103 64-65 0.6453042283242212 1103 66-67 0.634267614625351 1103 68-69 0.576681210364093 1103 70-71 0.5944900539840319 1103 72-73 0.649187080729412 1103 74-75 0.5618132133632514 1103 76-77 0.5834824026138108 1103 78-79 0.574704150384683 1103 80-81 0.5651901280709879 1103 82-83 0.507851762394445 1103 84-85 0.45946054023016103 1103 86-87 0.5714222522116188 1103 88-89 0.49637978651139036 1103 90-91 0.403324761100297 1103 92-93 0.48634637414600235 1103 94-95 0.48714300639791475 1103 96-97 0.5201988384977376 1103 98-99 0.5743112076111352 1103 100-101 0.645359643291318 1103 102-103 0.5851617778548395 1103 104-105 0.6525037313577364 1103 106-107 0.6257697968778011 1103 108-109 0.6205729912492117 1103 110-111 0.5897742307183407 1103 112-113 0.5684448953342596 1103 114-115 0.6240265243481495 1103 116-117 0.6558764825721326 1103 118-119 0.609962078184072 1103 120-121 0.5333356600762329 1103 122-123 0.5185958993944482 1103 124-125 0.5221753307875154 1104 1 0.2293895583581076 1104 2 0.26513509287843107 1104 3 0.23264192253827787 1104 4 0.2785845153776876 1104 5 0.35401467446888546 1104 6 0.2664712216442737 1104 7 0.33496727236035895 1104 8 0.2958735735582394 1104 9 0.31355464098395913 1104 10-11 0.28213051681258605 1104 12-13 0.28549292048406727 1104 14-15 0.3933931312047818 1104 16-17 0.3697590396161772 1104 18-19 0.3915961233556118 1104 20-21 0.3247416207284388 1104 22-23 0.29984664705081343 1104 24-25 0.40619531384557206 1104 26-27 0.402367085757362 1104 28-29 0.4206196487743341 1104 30-31 0.4487300339715361 1104 32-33 -0.25459381988993357 1104 34-35 0.34388528707259525 1104 36-37 0.4151695764893901 1104 38-39 0.5209553960753652 1104 40-41 0.568800779196259 1104 42-43 0.520593002245711 1104 44-45 0.4470914931524348 1104 46-47 0.5404663394916511 1104 48-49 0.49652709699580555 1104 50-51 0.522861546145279 1104 52-53 0.46517433300579825 1104 54-55 0.5365210432622263 1104 56-57 0.4364830396586328 1104 58-59 0.49735556632999334 1104 60-61 0.5051970509250765 1104 62-63 0.5933950032110786 1104 64-65 0.5337496103741586 1104 66-67 0.5113456457598602 1104 68-69 0.507694533464182 1104 70-71 0.577245049980867 1104 72-73 0.5670993995930473 1104 74-75 0.5068040273037937 1104 76-77 0.4737389163650576 1104 78-79 0.4721206540087408 1104 80-81 0.3638215311388464 1104 82-83 0.37229597928619285 1104 84-85 0.37548136594158876 1104 86-87 0.31736219288001877 1104 88-89 0.2655287438339897 1104 90-91 0.2892295283072599 1104 92-93 0.2817499642648258 1104 94-95 0.30605843500115526 1104 96-97 0.30570554312814835 1104 98-99 0.3030411056498501 1104 100-101 0.27733007955623634 1104 102-103 0.24882872553803992 1104 104-105 0.29695057283789694 1104 106-107 0.23223393943272086 1104 108-109 0.23683118977414352 1104 110-111 0.26509285972879226 1104 112-113 0.2894786517830603 1104 114-115 0.21831343825634875 1104 116-117 0.22769297612200745 1104 118-119 0.3053442606751524 1104 120-121 0.31107921657911675 1104 122-123 0.3911792897264199 1104 124-125 0.499947121499126 1105 1 0.3114205620899462 1105 2 0.2827250586106125 1105 3 0.3323336901272249 1105 4 0.3459840760988371 1105 5 0.37113053426571696 1105 6 0.23398250867029446 1105 7 0.19814106205561188 1105 8 0.2370351203892227 1105 9 0.1933184523380973 1105 10-11 0.28464175299615135 1105 12-13 0.3290835929264375 1105 14-15 0.3772924046660364 1105 16-17 0.34557982757261385 1105 18-19 0.35587281310475305 1105 20-21 0.33454296652833904 1105 22-23 0.27811918551313397 1105 24-25 0.3923555514832273 1105 26-27 0.41535066110080265 1105 28-29 0.42784665018776025 1105 30-31 0.33678777575286034 1105 32-33 0.042815311924229604 1105 34-35 0.38072477996990983 1105 36-37 0.35821655237989347 1105 38-39 0.3708619730559377 1105 40-41 0.35580099781883234 1105 42-43 0.2719575503070857 1105 44-45 0.2843013733676045 1105 46-47 0.35932946396416554 1105 48-49 0.3716395223844984 1105 50-51 0.397986110171189 1105 52-53 0.4745180331166381 1105 54-55 0.3556906206597006 1105 56-57 0.3470443531227332 1105 58-59 0.48849531445644345 1105 60-61 0.3839137618246866 1105 62-63 0.5189006950244206 1105 64-65 0.40843248053268155 1105 66-67 0.4619572169952306 1105 68-69 0.4485290997271605 1105 70-71 0.4259915291405463 1105 72-73 0.3320529931380918 1105 74-75 0.42635968962851223 1105 76-77 0.4487073304870606 1105 78-79 0.48018986076468195 1105 80-81 0.4969914695177877 1105 82-83 0.47208441110137045 1105 84-85 0.37321932136291736 1105 86-87 0.29956548899667723 1105 88-89 0.29285110028030914 1105 90-91 0.33331172022737476 1105 92-93 0.41201864124234433 1105 94-95 0.3706265155966193 1105 96-97 0.38326520675443376 1105 98-99 0.36703841608374077 1105 100-101 0.3065401529727474 1105 102-103 0.32750997382210656 1105 104-105 0.4489458216100566 1105 106-107 0.47289818345618784 1105 108-109 0.4945415758717786 1105 110-111 0.4359572705155266 1105 112-113 0.43947598255393316 1105 114-115 0.5061408001428092 1105 116-117 0.5657497010541306 1105 118-119 0.5728409304940492 1105 120-121 0.5388622057089947 1105 122-123 0.5661861534584567 1105 124-125 0.6363857851559196 1106 1 0.15520301627139332 1106 2 0.1197791794287113 1106 3 0.1621589703212436 1106 4 0.13368758070020448 1106 5 0.10636181814562917 1106 6 0.15802621713644527 1106 7 0.06767975494375378 1106 8 -0.03664630186180773 1106 9 0.1275227583513896 1106 10-11 0.1013118339177339 1106 12-13 0.09406035348136044 1106 14-15 0.18713134938362685 1106 16-17 0.13775244179633006 1106 18-19 0.17901928539151157 1106 20-21 0.22755747598619536 1106 22-23 0.22279456115621343 1106 24-25 0.2287718444578175 1106 26-27 0.24627844548196975 1106 28-29 0.31593919225437617 1106 30-31 0.31320784316751116 1106 32-33 0.23433587849544324 1106 34-35 0.32757514855936165 1106 36-37 0.30029784466412934 1106 38-39 0.3808700547099093 1106 40-41 0.3653866123246914 1106 42-43 0.3254281592000652 1106 44-45 0.31788282173206284 1106 46-47 0.2692903088384284 1106 48-49 0.35412739615161826 1106 50-51 0.2957029116071226 1106 52-53 0.2859851272029914 1106 54-55 0.34341187188282873 1106 56-57 0.24645046036184937 1106 58-59 0.3398607913115512 1106 60-61 0.37329392823021834 1106 62-63 0.2690783488384838 1106 64-65 0.31684884404352687 1106 66-67 0.3989767825519266 1106 68-69 0.32075698736140623 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2216 86-87 -1.897771912731237 2216 88-89 -1.967232956714529 2216 90-91 -1.9093035388899224 2216 92-93 -2.010167522549139 2216 94-95 -2.0564880894188136 2216 96-97 -1.905506210779354 2216 98-99 -1.9632149933036125 2216 100-101 -2.0048670972486384 2216 102-103 -2.042240122918063 2216 104-105 -1.9095995439809386 2216 106-107 -1.8938858580666569 2216 108-109 -1.9184170392786157 2216 110-111 -1.9165096279415508 2216 112-113 -1.9699110466398864 2216 114-115 -2.074421807875673 2216 116-117 -2.001630912891315 2216 118-119 -2.0462291705049402 2216 120-121 -2.0558017094517247 2216 122-123 -2.1350623496020056 2216 124-125 -2.379046402960366 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 47912.0 3 7951.0 4 4525.0 5 3674.0 6 3986.0 7 4722.0 8 5545.0 9 6282.0 10 6963.0 11 7727.0 12 8759.0 13 8648.0 14 8854.0 15 8095.0 16 7252.0 17 6745.0 18 6486.0 19 6634.0 20 6816.0 21 7379.0 22 8912.0 23 11334.0 24 14862.0 25 19144.0 26 23616.0 27 28282.0 28 34196.0 29 42243.0 30 51001.0 31 61826.0 32 79549.0 33 105723.0 34 143903.0 35 212106.0 36 393288.0 37 795360.0 38 423500.0 39 2694.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.851716518854474 18.594881352405086 12.946190852169442 24.607211276571004 2 15.549226319777626 19.62312996519558 40.52138928510364 24.306254429923158 3 19.103572780924768 23.64763299917001 29.7691710429185 27.479623176986717 4 12.35798714273038 16.11652780285112 38.1330338811391 33.3924511732794 5 13.302717200880693 37.79818598267687 35.44873060098504 13.450366215457398 6 32.54873488140315 36.74127617587386 17.409489355887782 13.300499586835219 7 28.164307709736157 32.15741416170215 21.40930422315929 18.268973905402394 8 25.397869151380885 36.29041271221602 20.111548807579073 18.200169328824025 9 25.575072326632352 16.00625312445693 20.289467297797398 38.12920725111332 10-11 24.57625171991611 26.303231260092662 28.725508662558006 20.395008357433213 12-13 24.94890038921277 23.83051909776762 28.423195327532376 22.797385185487233 14-15 22.04684893672609 25.83250792156052 25.745267878419 26.375375263294394 16-17 22.26426165954831 28.73962581349469 26.718358013453813 22.27775451350319 18-19 22.197681632003054 28.0228104350103 27.95047747600657 21.82903045698007 20-21 22.75172075858848 27.350854828460086 27.937137760219276 21.960286652732158 22-23 22.48559743256708 27.372908528980773 27.525763821822608 22.615730216629544 24-25 22.27006087352999 27.431447891132816 27.834393831563965 22.46409740377323 26-27 22.134280740349656 27.724289703170218 27.591397989433748 22.55003156704638 28-29 22.172811038298995 27.96995825536478 27.545583491854238 22.311647214481987 30-31 22.320571529741652 27.665982400482335 27.522200370365052 22.491245699410957 32-33 22.097898510737707 27.651198681298407 27.510190862991962 22.740711944971924 34-35 22.611414740099583 27.789210918087548 27.36643993065086 22.232934411162013 36-37 22.695883985219805 27.44752753112765 27.359890879838694 22.496697603813846 38-39 22.225218314743856 27.702789406760292 27.465154647140306 22.60683763135555 40-41 22.62613752975181 27.622796606830935 27.389084992543644 22.361980870873616 42-43 22.357977192501956 27.543599869636466 27.431367883750003 22.667055054111575 44-45 22.45597579129961 27.750605705249008 27.48789593671095 22.305522566740425 46-47 22.60412807211071 27.796056627048287 27.05483074567695 22.54498455516405 48-49 22.30502814500109 27.760054300415366 27.314673814894963 22.620243739688583 50-51 22.45163632482548 27.915080461555323 27.24193591269109 22.39134730092811 52-53 22.63991105094124 27.728985392760475 27.252221192117076 22.37888236418121 54-55 22.38606069890434 27.737573600610627 27.392977296385908 22.483388404099124 56-57 22.357681835504533 27.997517211153504 27.254478016782535 22.39032293655943 58-59 22.298439136418544 27.71867811233658 27.360707671051998 22.62217508019288 60-61 22.229374801463333 27.760594375510028 27.4966948007825 22.51333602224414 62-63 22.218328663613367 27.814934483650372 27.536836209891035 22.42990064284523 64-65 22.04861406662174 27.828862815998413 27.45791740731099 22.664605710068855 66-67 22.077063000122617 27.930024391722917 27.2665641720769 22.726348436077558 68-69 22.37650875605095 27.610989022341574 27.447723591280855 22.56477863032662 70-71 22.156264939627405 27.61634185521405 27.387389524474347 22.84000368068419 72-73 22.263461448317624 27.564546170278103 27.42784384365999 22.744148537744287 74-75 22.180619706583656 27.85601524932431 27.301230016253292 22.66213502783874 76-77 22.28301344291567 27.628175047342225 27.390923492399516 22.69788801734259 78-79 22.340885727618275 27.690787989663136 27.41476460143061 22.553561681287977 80-81 22.304862370577837 27.656301414939126 27.3315311799322 22.70730503455084 82-83 22.282128749163963 27.775953925669484 27.396599064249084 22.545318260917476 84-85 22.297979797979796 27.761000153045607 27.33478726660545 22.606232782369144 86-87 22.300452644834202 27.658525639958842 27.366792285507312 22.674229429699647 88-89 22.267026409495436 27.706593555007252 27.31888929146553 22.70749074403178 90-91 22.239833946959656 27.708915232730753 27.378285757130215 22.67296506317938 92-93 22.322731932734534 27.43303673894083 27.613135586736647 22.63109574158799 94-95 22.10540569591177 27.42457619954549 27.66850746731103 22.801510637231708 96-97 22.281520822643934 27.25425891421664 27.634516209413835 22.829704053725592 98-99 22.112686045451323 27.261062651511253 27.5413124753037 23.084938827733723 100-101 22.05205895031432 27.29937863027211 27.63612833421108 23.012434085202493 102-103 21.954679148371373 27.025053117839416 27.858232901942827 23.16203483184639 104-105 21.894184920725184 27.214223112557907 27.65027520866446 23.241316758052445 106-107 21.95555902169292 27.280826704345618 27.460084624724367 23.303529649237092 108-109 21.96526498790461 27.163798017862256 27.522301056908177 23.34863593732496 110-111 21.98731359565466 27.23511686058818 27.583645923384033 23.193923620373127 112-113 21.98381583267348 27.369098562263574 27.61723998738737 23.02984561767558 114-115 21.910945912873224 27.43365302251591 27.534225097895256 23.121175966715615 116-117 21.898789477256457 27.351084098015725 27.61966853300474 23.130457891723076 118-119 21.84562546574632 27.467173969925984 27.450380324427222 23.236820239900478 120-121 21.77513056846508 27.661216456092525 27.517331508967324 23.046321466475074 122-123 21.856948006434717 27.535753630043562 27.46763235850258 23.13966600501914 124-125 21.818261779063562 27.50412537310008 27.50886740104363 23.168745446792727 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 10771.0 1 5625.5 2 479.5 3 434.5 4 387.0 5 341.0 6 307.5 7 280.5 8 256.0 9 277.0 10 286.5 11 283.0 12 284.0 13 302.0 14 343.0 15 398.5 16 476.5 17 612.0 18 763.5 19 989.5 20 1389.5 21 2054.5 22 3008.0 23 4269.5 24 6085.0 25 8700.5 26 12888.0 27 18280.5 28 22979.0 29 28162.5 30 34072.5 31 40419.5 32 48672.5 33 54144.0 34 57412.0 35 62097.5 36 67723.0 37 73135.5 38 76811.5 39 80418.5 40 83967.0 41 88010.5 42 91111.5 43 93296.5 44 95957.5 45 99286.5 46 100546.5 47 100333.5 48 99089.0 49 96474.0 50 94463.0 51 91489.5 52 89277.5 53 88619.5 54 87065.5 55 80481.5 56 71877.5 57 64390.0 58 58389.5 59 51274.5 60 41514.5 61 33837.5 62 27909.0 63 20726.5 64 14626.5 65 11088.5 66 8165.0 67 6219.0 68 4676.5 69 3207.5 70 2249.5 71 1558.0 72 1076.0 73 815.0 74 599.5 75 448.5 76 359.5 77 232.5 78 132.0 79 77.0 80 61.5 81 50.5 82 36.0 83 35.0 84 24.0 85 18.0 86 20.0 87 15.5 88 13.5 89 11.0 90 7.0 91 7.0 92 7.0 93 6.0 94 7.5 95 5.5 96 3.5 97 3.0 98 2.5 99 3.0 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4708177517253037 2 0.4633934058101789 3 0.4570351198213284 4 0.45467455855600664 5 0.4639645093421116 6 0.5229404674063599 7 0.518409712719694 8 0.5264051621667516 9 0.5345529058889912 10-11 0.5500869219575601 12-13 0.5590532474089033 14-15 0.5391788444976459 16-17 0.5330299631371707 18-19 0.5432527163587657 20-21 0.543462120987141 22-23 0.5538371684839182 24-25 0.5466222271971686 26-27 0.5556075894329094 28-29 0.5354095611868902 30-31 0.5408921550934439 32-33 0.5416917000381497 34-35 0.5408350447402507 36-37 0.48968320506348006 38-39 0.45602617024824726 40-41 0.4548649263999842 42-43 0.4670103948457525 44-45 0.47182670129838483 46-47 0.4764336031226418 48-49 0.49061600749897005 50-51 0.47285468765586364 52-53 0.47262624624309063 54-55 0.4618514262739607 56-57 0.44517520314152637 58-59 0.4634314793789744 60-61 0.45888168790791073 62-63 0.47553887425594726 64-65 0.4758244260219136 66-67 0.48343913978101605 68-69 0.49337634123664476 70-71 0.4892263222379339 72-73 0.5007245400141785 74-75 0.49000683039824194 76-77 0.4878176001925 78-79 0.4998488479318818 80-81 0.493205010177065 82-83 0.4941187758281573 84-85 0.4909205960493342 86-87 0.4810405049468988 88-89 0.4863898413626683 90-91 0.47171248059199833 92-93 0.47112234027566785 94-95 0.46575396707550065 96-97 0.45492203675317744 98-99 0.4176860864711665 100-101 0.4150590102242762 102-103 0.4040367120579754 104-105 0.40934797490494934 106-107 0.40776792179993554 108-109 0.42014183165847707 110-111 0.44346189254572826 112-113 0.4437284075272968 114-115 0.4360946569837967 116-117 0.430288437742481 118-119 0.4724929887523063 120-121 0.4664964016670131 122-123 0.43042169523326534 124-125 0.44085385308323566 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2626494.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 74.40053328372801 #Duplication Level Percentage of deduplicated Percentage of total 1 84.32416199097952 62.73762620832344 2 10.16265721374389 15.122142325645417 3 2.820758904378786 6.29597900251818 4 1.1064765411311 3.292897789043546 5 0.5379267533869321 2.001101865978609 6 0.3067575546133214 1.369375539122606 7 0.18274100119053885 0.9517219558964926 8 0.11555685147275456 0.6877993099329193 9 0.08169861705640344 0.5470578609785545 >10 0.3335113668852895 4.237138696902824 >50 0.01989476790695374 1.0114645442669625 >100 0.007654330157263152 0.8804150323735321 >500 1.0205354865543327E-4 0.040621685672516984 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 1.0205354865543327E-4 0.8246581833443121 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 10861 0.41351703068805795 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 3.8073568795512195E-5 2 0.0 0.0 0.0 0.0 3.8073568795512195E-5 3 0.0 0.0 0.0 0.0 3.8073568795512195E-5 4 0.0 0.0 0.0 0.0 3.8073568795512195E-5 5 0.0 0.0 0.0 0.0 3.8073568795512195E-5 6 0.0 0.0 0.0 0.0 7.614713759102439E-5 7 0.0 0.0 0.0 0.0 7.614713759102439E-5 8 0.0 0.0 0.0 0.0 7.614713759102439E-5 9 0.0 0.0 0.0 0.0 7.614713759102439E-5 10-11 0.0 0.0 0.0 0.0 7.614713759102439E-5 12-13 0.0 0.0 0.0 0.0 3.99772472352878E-4 14-15 0.0 0.0 0.0 0.0 5.901403163304389E-4 16-17 0.0 0.0 0.0 5.711035319326829E-5 7.99544944705756E-4 18-19 0.0 0.0 0.0 7.614713759102439E-5 7.99544944705756E-4 20-21 0.0 0.0 0.0 1.5229427518204878E-4 9.518392198878047E-4 22-23 0.0 0.0 0.0 2.4747819717082923E-4 0.0010279863574788291 24-25 0.0 0.0 0.0 2.8555176596634145E-4 0.0010279863574788291 26-27 0.0 0.0 0.0 5.520667475349268E-4 0.0012373909858541463 28-29 0.0 0.0 0.0 0.0013135381234451707 0.0013135381234451707 30-31 0.0 0.0 0.0 0.005006674296609854 0.0013325749078429267 32-33 0.0 0.0 0.0 0.014525066495487902 0.0013896852610361951 34-35 0.0 0.0 0.0 0.029830641151283803 0.0014087220454339511 36-37 0.0 0.0 0.0 0.05604429326699394 0.0014087220454339511 38-39 0.0 0.0 0.0 0.0941368988469039 0.0014277588298317071 40-41 0.0 0.0 0.0 0.13335267470628145 0.0014467956142294634 42-43 0.0 0.0 0.0 0.174053319748684 0.0014658323986272196 44-45 0.0 0.0 0.0 0.226423513626911 0.0014848691830249756 46-47 0.0 0.0 0.0 0.29312840615664837 0.0015039059674227316 48-49 0.0 0.0 0.0 0.3651826351021552 0.0015610163206159998 50-51 0.0 0.0 0.0 0.4454988284762882 0.0017133105957980487 52-53 0.0 0.0 0.0 0.5215507821453238 0.0020940462837531707 54-55 0.0 0.0 0.0 0.6085488868430691 0.002170193421344195 56-57 0.0 0.0 0.0 0.7143934080945931 0.002208266990139707 58-59 0.0 0.0 0.0 0.8372187410289154 0.002246340558935219 60-61 0.0 0.0 0.0 0.9731223448444961 0.0023415244809239996 62-63 0.0 0.0 0.0 1.1106821489026817 0.002398634834117268 64-65 0.0 0.0 0.0 1.2543337239681494 0.0024367084029127805 66-67 0.0 0.0 0.0 1.4243702822089066 0.0024367084029127805 68-69 0.0 0.0 0.0 1.626712263572656 0.0024367084029127805 70-71 0.0 0.0 0.0 1.8453878059496804 0.0024557451873105365 72-73 0.0 0.0 0.0 2.0714115471042387 0.0026270762468903414 74-75 0.0 0.0 0.0 2.301718564748292 0.0028174440908679023 76-77 0.0 0.0 0.0 2.5618752603280264 0.0028745544440611703 78-79 0.0 0.0 0.0 2.860771812157195 0.003007811934845463 80-81 0.0 0.0 0.0 3.186148531083642 0.003064922288038731 82-83 0.0 0.0 0.0 3.5283728042021036 0.0031220326412319996 84-85 0.0 0.0 0.0 3.8716631372468395 0.0031220326412319996 86-87 0.0 0.0 0.0 4.244232044695323 0.0032743269164140485 88-89 0.0 0.0 0.0 4.652590106811591 0.0034266211915960974 90-91 0.0 0.0 0.0 5.10918738059177 0.0034266211915960974 92-93 0.0 0.0 0.0 5.578367968858867 0.003578915466778146 94-95 0.0 0.0 0.0 6.052707525697755 0.0036740993887669268 96-97 0.0 0.0 0.0 6.542029031857679 0.004188092567506341 98-99 0.0 0.0 0.0 7.066739920213029 0.004454607549074926 100-101 0.0 0.0 0.0 7.645020319863667 0.004454607549074926 102-103 0.0 0.0 0.0 8.251874932895335 0.004568828255461463 104-105 0.0 0.0 0.0 8.857035272115603 0.004683048961847999 106-107 0.0 0.0 0.0 9.459911197208143 0.004683048961847999 108-109 0.0 0.0 0.0 10.090257202186642 0.004740159315041268 110-111 0.0 0.0 0.0 10.781711285081938 0.004797269668234536 112-113 0.0 0.0 0.0 11.508821265154232 0.004797269668234536 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2620 0.0 50.879894 1 GTATCAA 4145 0.0 40.91851 1 TCAACGC 4185 0.0 39.542606 4 ATCAACG 4305 0.0 38.710247 3 CAACGCA 4325 0.0 38.40025 5 AACGCAG 4695 0.0 35.375385 6 TATCAAC 4790 0.0 34.663166 2 CGCAGAG 5405 0.0 30.06938 8 ACGCAGA 5500 0.0 29.874725 7 TACATGG 7090 0.0 27.195576 2 GTACATG 7430 0.0 26.511766 1 ACATGGG 7220 0.0 25.471958 3 GAGTACA 5790 0.0 21.789978 1 GCAGAGT 7110 0.0 21.435219 9 TGGTATC 1690 0.0 21.128275 2 CATGGGG 4625 0.0 20.46451 4 CAGAGTA 6470 0.0 20.151615 10-11 AGAGTAC 6050 0.0 19.238066 10-11 GTGTAGC 2330 0.0 19.156 1 TAGACCG 250 1.2576713E-5 19.048725 5 >>END_MODULE