FastQCFastQC Report
Thu 26 May 2016
SRR1364116_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364116_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2626494
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51110.19459401011386282No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47990.182715056649663No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26370.10040000091376565No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA41700.071.454151
GTATCAA70250.047.6742061
ATCAACG84750.038.750853
TCAACGC85450.038.5030364
CAACGCA86750.037.857465
TATCAAC90150.036.694352
AACGCAG91600.035.917956
TGGTATC17450.033.0724562
ACGCAGA104750.031.0710937
CGCAGAG104650.030.9870688
GTGGTAT20750.029.5809861
GTACATG72700.027.0503581
GCAGAGT124100.025.9866739
TACATGG72800.025.9070552
GAGTACT90700.025.19110112-13
ACATGGG72100.025.0028343
CAGAGTA120500.024.7138410-11
GTACTTT96750.023.45986214-15
AGAGTAC115300.023.42878710-11
AGTACTT96800.021.23713512-13
Produced by FastQC (version 0.11.5)