FastQCFastQC Report
Thu 26 May 2016
SRR1364115_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364115_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1786165
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN69490.3890458048388587No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA32050.050.315921
GGTATCA19600.049.1839181
ATCAACG37250.042.8199353
TCAACGC37450.042.5960544
CAACGCA38400.041.545755
AACGCAG39600.040.438256
TATCAAC41750.038.9108162
GTACATG73750.032.0326351
CGCAGAG48900.031.6527868
ACGCAGA49700.031.6224147
TACATGG72700.031.5130542
ACATGGG74600.030.2369423
CATGGGG43150.025.3818574
GCAGAGT59850.024.6680589
GTGGTAT14650.023.5588741
TAGATCG1300.002046944322.8954755
CATGGGA35100.022.554384
TGGTATC13550.022.3972682
GAGTACA58550.022.257761
AGAGTAC49850.021.61588910-11
Produced by FastQC (version 0.11.5)