FastQCFastQC Report
Thu 26 May 2016
SRR1364115_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364115_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1786165
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51330.2873754664322725No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50690.28379237080560865No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27990.15670444779737594No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18040.1009985079765867No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA37550.074.4281161
GTATCAA63550.049.9787981
TGGTATC15800.041.7971342
ATCAACG81750.038.4250643
TCAACGC83150.037.77814
GTGGTAT18450.037.4658661
TATCAAC83800.037.4151272
CAACGCA85250.036.8474965
AACGCAG88550.035.7430426
ACGCAGA105550.029.7607677
CGCAGAG105100.029.7183728
GTACATG71950.029.3188271
TACATGG71000.028.8257562
ACATGGG72150.027.2935543
GAGTACT74850.026.70718412-13
GCAGAGT114850.026.573879
CAGAGTA111200.025.76119610-11
GTACTTT81550.024.5129714-15
AGAGTAC107100.023.99765610-11
AGTACTT79500.022.33869412-13
Produced by FastQC (version 0.11.5)