##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364115_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1786165 Sequences flagged as poor quality 0 Sequence length 125 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.020743884243615 33.0 31.0 34.0 30.0 34.0 2 32.07787970316292 33.0 31.0 34.0 30.0 34.0 3 32.200994868895094 34.0 31.0 34.0 30.0 34.0 4 35.846515299538396 37.0 35.0 37.0 35.0 37.0 5 35.78490788924876 37.0 35.0 37.0 35.0 37.0 6 35.82326996666041 37.0 35.0 37.0 35.0 37.0 7 35.754801488104405 37.0 35.0 37.0 33.0 37.0 8 35.73377711465626 37.0 35.0 37.0 33.0 37.0 9 37.38072518496332 39.0 37.0 39.0 34.0 39.0 10-11 37.300404777834075 39.0 37.0 39.0 33.5 39.0 12-13 37.245981474275894 39.0 37.0 39.0 33.0 39.0 14-15 38.55099668843584 40.0 38.0 41.0 34.0 41.0 16-17 38.51994216659715 40.0 38.0 41.0 33.0 41.0 18-19 38.65119459848334 40.0 38.0 41.0 34.0 41.0 20-21 38.70713064022641 40.0 38.0 41.0 34.0 41.0 22-23 38.683466253117714 40.0 38.0 41.0 34.0 41.0 24-25 38.64780913297484 40.0 38.0 41.0 34.0 41.0 26-27 38.52759067611335 40.0 38.0 41.0 34.0 41.0 28-29 38.452717134195325 40.0 38.0 41.0 34.0 41.0 30-31 38.32236215579188 40.0 38.0 41.0 34.0 41.0 32-33 38.189408873200406 40.0 37.5 41.0 33.5 41.0 34-35 38.07289696080709 40.0 37.0 41.0 33.0 41.0 36-37 37.96188454034201 40.0 37.0 41.0 33.0 41.0 38-39 37.85943515856598 40.0 37.0 41.0 33.0 41.0 40-41 37.72360448222868 40.0 37.0 41.0 33.0 41.0 42-43 37.578999980404944 40.0 37.0 41.0 32.5 41.0 44-45 37.41045620085491 40.0 37.0 41.0 32.0 41.0 46-47 37.1621476739271 40.0 36.0 41.0 31.0 41.0 48-49 36.90968751487125 40.0 36.0 41.0 31.0 41.0 50-51 36.71890362872411 40.0 36.0 41.0 30.5 41.0 52-53 36.55492885595676 39.5 35.5 41.0 30.0 41.0 54-55 36.38156749236492 39.0 35.0 41.0 30.0 41.0 56-57 36.184949318791936 39.0 35.0 41.0 30.0 41.0 58-59 35.975776314058336 39.0 35.0 41.0 29.0 41.0 60-61 35.73851855791598 38.0 35.0 40.5 29.0 41.0 62-63 35.47763112590383 38.0 35.0 40.0 29.0 41.0 64-65 35.262040181058296 37.0 34.5 40.0 28.5 41.0 66-67 35.06710634235919 37.0 34.0 40.0 29.0 41.0 68-69 34.75971676748789 36.5 34.0 39.0 28.5 41.0 70-71 34.45019189156657 36.0 34.0 39.0 28.0 41.0 72-73 34.12662519979958 35.5 34.0 39.0 28.0 40.5 74-75 33.85139250853085 35.0 34.0 37.5 28.5 40.0 76-77 33.656205333773755 35.0 34.0 37.0 29.0 39.0 78-79 33.385561524271274 35.0 34.0 37.0 29.0 39.0 80-81 33.13202335730462 35.0 34.0 36.0 28.0 38.5 82-83 32.88866454106983 35.0 34.0 36.0 28.0 37.0 84-85 32.68917457233795 35.0 33.5 35.5 28.0 37.0 86-87 32.51369946225573 35.0 33.0 35.0 27.0 36.5 88-89 32.37925779533246 35.0 33.0 35.0 27.0 36.0 90-91 32.2543684374064 35.0 33.0 35.0 27.0 36.0 92-93 32.1562691576646 35.0 33.0 35.0 27.0 36.0 94-95 32.07683724627904 35.0 33.0 35.0 27.0 35.5 96-97 32.02074640360773 35.0 33.0 35.0 27.0 35.0 98-99 31.965787315281624 35.0 33.0 35.0 27.0 35.0 100-101 31.90531333891326 35.0 33.0 35.0 26.5 35.0 102-103 30.711871803556782 34.0 31.0 34.5 24.0 35.0 104-105 31.660440384846865 35.0 33.0 35.0 25.0 35.0 106-107 31.820359821181135 35.0 33.0 35.0 26.0 35.0 108-109 31.82265636153435 35.0 33.0 35.0 26.0 35.0 110-111 31.7866347733832 35.0 33.0 35.0 25.5 35.0 112-113 31.733025224433355 35.0 33.0 35.0 25.0 35.0 114-115 31.666473981966952 35.0 33.0 35.0 25.0 35.0 116-117 31.60447299101707 35.0 33.0 35.0 25.0 35.0 118-119 31.518168254332608 35.0 33.0 35.0 25.0 35.0 120-121 31.412559310030147 35.0 33.0 35.0 25.0 35.0 122-123 31.276314338261024 35.0 33.0 35.0 24.5 35.0 124-125 30.427800623122724 34.5 31.5 35.0 21.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.34794139858658113 1101 2 0.38546201494539645 1101 3 0.2578750168167616 1101 4 0.24269829046102842 1101 5 0.23452606974566947 1101 6 0.33061377594249564 1101 7 0.31687457811195685 1101 8 0.37932477854114666 1101 9 0.39088571128507255 1101 10-11 0.4313014354545217 1101 12-13 0.4349960872199219 1101 14-15 0.5060246070522467 1101 16-17 0.5720329332821521 1101 18-19 0.5528855615734543 1101 20-21 0.511724007695598 1101 22-23 0.507859830996864 1101 24-25 0.4846964205377091 1101 26-27 0.5646976026554285 1101 28-29 0.5996737217187942 1101 30-31 0.6596049995091064 1101 32-33 0.6239775065758693 1101 34-35 0.5680964550375194 1101 36-37 0.6880009166910668 1101 38-39 0.6473475965345088 1101 40-41 0.6396063673532169 1101 42-43 0.6114912856110877 1101 44-45 0.7146964263428046 1101 46-47 0.7914374863148481 1101 48-49 0.755160600656474 1101 50-51 0.7286089742783588 1101 52-53 0.7303857315689513 1101 54-55 0.6841825385018439 1101 56-57 0.6423013347150288 1101 58-59 0.5939194585366181 1101 60-61 0.639255992659109 1101 62-63 0.6482401877468646 1101 64-65 0.6219733473299911 1101 66-67 0.6178150208176589 1101 68-69 0.6248676682013823 1101 70-71 0.5978909513724986 1101 72-73 0.5937584975558607 1101 74-75 0.6313817783783833 1101 76-77 0.6482305849437253 1101 78-79 0.5954961199285052 1101 80-81 0.5985615455389421 1101 82-83 0.6364469415605569 1101 84-85 0.5871109235026566 1101 86-87 0.643150126602265 1101 88-89 0.5721763922807028 1101 90-91 0.532641020066059 1101 92-93 0.48470361195320777 1101 94-95 0.532537387222078 1101 96-97 0.6114400119702275 1101 98-99 0.6291583300247012 1101 100-101 0.6282461428034658 1101 102-103 0.6918349957733092 1101 104-105 0.6644352534156504 1101 106-107 0.6890017275218554 1101 108-109 0.7219871437966852 1101 110-111 0.7185782192710874 1101 112-113 0.7838006227597774 1101 114-115 0.7239701752997085 1101 116-117 0.7563494990333801 1101 118-119 0.6908217726006178 1101 120-121 0.6703677483118611 1101 122-123 0.6008267218791978 1101 124-125 0.6931135079831847 1102 1 0.4509987029755571 1102 2 0.4328387800440865 1102 3 0.3709233187228591 1102 4 0.27252660840807863 1102 5 0.31688611333760264 1102 6 0.30643987135876216 1102 7 0.25742039531451155 1102 8 0.22561971559228056 1102 9 0.38472453715303345 1102 10-11 0.3968011097939339 1102 12-13 0.4007586765367961 1102 14-15 0.42512369876521205 1102 16-17 0.4841991197554947 1102 18-19 0.537234841009429 1102 20-21 0.6047223734549192 1102 22-23 0.6134766312467903 1102 24-25 0.564792483561483 1102 26-27 0.6042814079606416 1102 28-29 0.5914848764847633 1102 30-31 0.6517530622624861 1102 32-33 0.5909447660392146 1102 34-35 0.7056640764271549 1102 36-37 0.733738645270094 1102 38-39 0.66262058735024 1102 40-41 0.6875494416074872 1102 42-43 0.7139288985417735 1102 44-45 0.7651461279956848 1102 46-47 0.724861533403633 1102 48-49 0.737499679562255 1102 50-51 0.7898030775835636 1102 52-53 0.7229313925640426 1102 54-55 0.6879252374014584 1102 56-57 0.7220096766504298 1102 58-59 0.6688185973858651 1102 60-61 0.660973454918107 1102 62-63 0.6838761576673278 1102 64-65 0.6930781177780929 1102 66-67 0.6805722264579401 1102 68-69 0.6472052215722641 1102 70-71 0.6579723048599462 1102 72-73 0.6476667443965383 1102 74-75 0.6463196311012354 1102 76-77 0.5872187807749043 1102 78-79 0.6057997036566221 1102 80-81 0.600223476500723 1102 82-83 0.5430077578770138 1102 84-85 0.5242905725930953 1102 86-87 0.5525947291319113 1102 88-89 0.5817404455629784 1102 90-91 0.645166004125592 1102 92-93 0.5870065987130317 1102 94-95 0.5974475839155957 1102 96-97 0.5760980158215183 1102 98-99 0.5547227322616664 1102 100-101 0.5561529148081696 1102 102-103 0.6177677644455812 1102 104-105 0.5287729520320745 1102 106-107 0.47742493876367575 1102 108-109 0.5204301276150609 1102 110-111 0.48417384473125935 1102 112-113 0.541705599748866 1102 114-115 0.5504747226725861 1102 116-117 0.5864037697089408 1102 118-119 0.5279778307098013 1102 120-121 0.5119224510700668 1102 122-123 0.5585583862286363 1102 124-125 0.755297610356628 1103 1 0.3471666030005487 1103 2 0.37604021154252365 1103 3 0.25117193347535505 1103 4 0.22182846556110292 1103 5 0.2898271484098629 1103 6 0.21445854779742746 1103 7 0.23970717575666356 1103 8 0.28744465260160723 1103 9 0.4021722927510396 1103 10-11 0.35223207137554624 1103 12-13 0.3385955238466707 1103 14-15 0.31223292623771925 1103 16-17 0.4663998068601529 1103 18-19 0.457500380229682 1103 20-21 0.444818538002437 1103 22-23 0.4682856340327959 1103 24-25 0.4462222304858514 1103 26-27 0.4967593247344908 1103 28-29 0.5183336482350214 1103 30-31 0.5613005511193805 1103 32-33 0.5586606767265607 1103 34-35 0.4552649682401011 1103 36-37 0.5116940071859659 1103 38-39 0.5442391124470802 1103 40-41 0.6492921281210045 1103 42-43 0.5719483275844439 1103 44-45 0.5555030926388014 1103 46-47 0.5749832385945055 1103 48-49 0.6455505127177048 1103 50-51 0.7353138060677509 1103 52-53 0.7407250039138518 1103 54-55 0.7838638896838006 1103 56-57 0.7950265764875013 1103 58-59 0.7045164870842271 1103 60-61 0.7165066794203412 1103 62-63 0.8498594370868489 1103 64-65 0.7744494352638682 1103 66-67 0.7282686766300799 1103 68-69 0.693425712653216 1103 70-71 0.6454150499397429 1103 72-73 0.6473536155160673 1103 74-75 0.5720283218074513 1103 76-77 0.5761591508188957 1103 78-79 0.5753497445252975 1103 80-81 0.5321934904763523 1103 82-83 0.5452846700841647 1103 84-85 0.4556759500901393 1103 86-87 0.36671117430493894 1103 88-89 0.29619267755841605 1103 90-91 0.41997377081651877 1103 92-93 0.3490337809453763 1103 94-95 0.42949481962387637 1103 96-97 0.42955421289977025 1103 98-99 0.43656800782420646 1103 100-101 0.5061937492772479 1103 102-103 0.5698029425253779 1103 104-105 0.638043072102068 1103 106-107 0.591730378767906 1103 108-109 0.4986071770241942 1103 110-111 0.47955057549241786 1103 112-113 0.4529742141547075 1103 114-115 0.4659884228023081 1103 116-117 0.5176889710510473 1103 118-119 0.4934886686636659 1103 120-121 0.5414525798611365 1103 122-123 0.47254880443928116 1103 124-125 0.5336337784983982 1104 1 0.24298315696579564 1104 2 0.30643065345514486 1104 3 0.21709233774626568 1104 4 0.2078477562412857 1104 5 0.23316741721533418 1104 6 0.2776381122050253 1104 7 0.2717774421570951 1104 8 0.27581550180542536 1104 9 0.30320478320077626 1104 10-11 0.29086834920832416 1104 12-13 0.25171824814487564 1104 14-15 0.28676202026370845 1104 16-17 0.328341788000138 1104 18-19 0.3323789045961192 1104 20-21 0.3321987403056852 1104 22-23 0.4746894099499315 1104 24-25 0.4074877583927261 1104 26-27 0.44310205171738914 1104 28-29 0.4734862426049844 1104 30-31 0.4924576004115764 1104 32-33 0.46798846727529764 1104 34-35 0.42446521461715747 1104 36-37 0.45949549048209803 1104 38-39 0.33675069021106196 1104 40-41 0.419395384157383 1104 42-43 0.46750217956804363 1104 44-45 0.5193330673019503 1104 46-47 0.5334604200521795 1104 48-49 0.6045230351041724 1104 50-51 0.5669501510180055 1104 52-53 0.5136255204387297 1104 54-55 0.5081379722718538 1104 56-57 0.5520637735499889 1104 58-59 0.6292814710599259 1104 60-61 0.5117732196824321 1104 62-63 0.5705374913513239 1104 64-65 0.5011491852512862 1104 66-67 0.3879430660009149 1104 68-69 0.41712719930523434 1104 70-71 0.25655669017890403 1104 72-73 0.3567014014784533 1104 74-75 0.4159219951467037 1104 76-77 0.323201437623041 1104 78-79 0.30864937929438696 1104 80-81 0.2431547175515405 1104 82-83 0.21741546805497336 1104 84-85 0.2803541008359858 1104 86-87 0.29562258483455395 1104 88-89 0.3936066972797647 1104 90-91 0.3413220559651009 1104 92-93 0.2521089846748623 1104 94-95 0.34998805614025486 1104 96-97 0.31873959555326437 1104 98-99 0.34056695072401055 1104 100-101 0.39773593400690643 1104 102-103 0.37018404392309634 1104 104-105 0.38345374014463474 1104 106-107 0.3828539881777786 1104 108-109 0.3589640322350256 1104 110-111 0.3419055737865122 1104 112-113 0.3963644714942802 1104 114-115 0.39173447545596574 1104 116-117 0.42600335768124253 1104 118-119 0.45718248574618414 1104 120-121 0.5002985578466088 1104 122-123 0.5343152314406403 1104 124-125 0.5544578482222633 1105 1 0.18505304342685847 1105 2 0.26199027817278875 1105 3 0.20669547329968196 1105 4 0.15736001591221793 1105 5 0.20454971468289074 1105 6 0.2332182380446426 1105 7 0.23119174373977813 1105 8 0.20116113208446507 1105 9 0.3674720731015526 1105 10-11 0.3088797496958833 1105 12-13 0.28776294804572444 1105 14-15 0.3689153742472584 1105 16-17 0.38627632791769173 1105 18-19 0.3106558691627228 1105 20-21 0.37197301363409707 1105 22-23 0.38196796055787985 1105 24-25 0.40988918527082063 1105 26-27 0.37177165936319057 1105 28-29 0.350224644561159 1105 30-31 0.37224554426087053 1105 32-33 0.43957111661665493 1105 34-35 0.4573320076452845 1105 36-37 0.38690288132293915 1105 38-39 0.48870547219095073 1105 40-41 0.5738189205636459 1105 42-43 0.556409255437103 1105 44-45 0.515474197025604 1105 46-47 0.5294032946076825 1105 48-49 0.5708720741606825 1105 50-51 0.6883002524053694 1105 52-53 0.7300891794044375 1105 54-55 0.620303803983731 1105 56-57 0.6548941398118586 1105 58-59 0.5973982659517958 1105 60-61 0.5306789816159494 1105 62-63 0.6521394117473278 1105 64-65 0.5988267808796977 1105 66-67 0.6144639230969133 1105 68-69 0.6082167593884336 1105 70-71 0.6210377508696823 1105 72-73 0.6053492592975758 1105 74-75 0.5855662168122961 1105 76-77 0.5154111044611653 1105 78-79 0.5520321762438201 1105 80-81 0.525861695015216 1105 82-83 0.5589841952707744 1105 84-85 0.5734956834480514 1105 86-87 0.5487424828507841 1105 88-89 0.5514532221532136 1105 90-91 0.5057277750326392 1105 92-93 0.4938491292264118 1105 94-95 0.5899991174274177 1105 96-97 0.5945116025758317 1105 98-99 0.6682177457947986 1105 100-101 0.6215375968342762 1105 102-103 0.637104458567606 1105 104-105 0.5880132362643344 1105 106-107 0.5866815010562121 1105 108-109 0.6122810205829374 1105 110-111 0.5471977520493638 1105 112-113 0.5941467160316662 1105 114-115 0.6056445798998702 1105 116-117 0.6425133771386733 1105 118-119 0.7216951833956351 1105 120-121 0.7186921178850731 1105 122-123 0.7448484749417865 1105 124-125 0.7954604183174645 1106 1 0.15871460581927366 1106 2 0.24365159284975135 1106 3 0.19398776115233574 1106 4 0.1826438413675362 1106 5 0.13623124731302738 1106 6 0.10572081993190352 1106 7 0.16264741815423633 1106 8 0.13627197564668592 1106 9 0.2135314847409049 1106 10-11 0.1753010080539994 1106 12-13 0.20452599909878444 1106 14-15 0.22379532331987662 1106 16-17 0.27900574248955223 1106 18-19 0.2209760413978259 1106 20-21 0.2607731327560927 1106 22-23 0.26370545684670077 1106 24-25 0.27287384694796657 1106 26-27 0.30845850148283205 1106 28-29 0.33735436914164296 1106 30-31 0.2795571575624578 1106 32-33 0.2657406614577056 1106 34-35 0.2716656257299732 1106 36-37 0.24460696881021704 1106 38-39 0.1678702339378546 1106 40-41 0.3645177969190243 1106 42-43 0.4162619558088565 1106 44-45 0.39305084765754117 1106 46-47 0.466463303678438 1106 48-49 0.4210121882190734 1106 50-51 0.4481499018697761 1106 52-53 0.38242212173590673 1106 54-55 0.42479141547845956 1106 56-57 0.5842656346835966 1106 58-59 0.6026838490940349 1106 60-61 0.5794764232252234 1106 62-63 0.5307731790077241 1106 64-65 0.5674516139905066 1106 66-67 0.5755951357916018 1106 68-69 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70-71 -2.4289046256887588 2216 72-73 -2.3474802828218273 2216 74-75 -2.2711125389513924 2216 76-77 -2.1427077266860195 2216 78-79 -2.102241150230604 2216 80-81 -2.111433008035924 2216 82-83 -1.9914111864683974 2216 84-85 -1.892768129783196 2216 86-87 -2.0404236113441883 2216 88-89 -1.9965966382496987 2216 90-91 -2.1323433931428823 2216 92-93 -2.0779757761410593 2216 94-95 -2.2335273458155953 2216 96-97 -2.2597448991437368 2216 98-99 -2.416045652461662 2216 100-101 -2.282004551595538 2216 102-103 -2.26803066625272 2216 104-105 -2.2346207893028414 2216 106-107 -2.456653934027649 2216 108-109 -2.4043249587091786 2216 110-111 -2.262458098396305 2216 112-113 -2.277398430752225 2216 114-115 -2.1748461224073274 2216 116-117 -2.3269396140662266 2216 118-119 -2.5362158109635047 2216 120-121 -2.7737034154381526 2216 122-123 -2.747654161653184 2216 124-125 -2.8103588236117574 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7.0 3 18.0 4 47.0 5 114.0 6 327.0 7 742.0 8 1355.0 9 2249.0 10 3556.0 11 5016.0 12 6498.0 13 9093.0 14 12866.0 15 11895.0 16 6002.0 17 4595.0 18 4590.0 19 4168.0 20 4022.0 21 4313.0 22 4698.0 23 5627.0 24 7277.0 25 9766.0 26 12999.0 27 17323.0 28 22577.0 29 29002.0 30 37090.0 31 46043.0 32 59063.0 33 76611.0 34 103276.0 35 153640.0 36 296680.0 37 571077.0 38 250687.0 39 1256.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.018668999590616 19.04327686087137 14.097422006875396 23.840632132662616 2 16.8744817686128 20.432411429671976 38.102247157670554 24.59085964404467 3 19.90902296260424 23.840742596568624 29.637351532473204 26.61288290835393 4 13.702429506792486 16.48638283697195 35.77183518879835 34.03935246743722 5 13.65825665602002 37.5980382551444 34.2304322389029 14.513272849932676 6 33.257677762132836 35.97612762538735 16.32251219792124 14.443682414558564 7 28.583921418234038 32.533052657509245 20.430363376283825 18.452662547972892 8 26.559920276122305 35.17754518759465 19.032900096015766 19.229634440267276 9 26.24271903363857 15.68920882964834 19.571159199267257 38.496912937445835 10-11 25.373539182983812 26.21114705417998 27.057572734326975 21.357741028509235 12-13 26.04436374610144 23.45911469028045 27.725703644666755 22.770817918951355 14-15 22.943883388120188 25.084559680813275 25.698080999818323 26.273475931248214 16-17 22.503213605082646 28.374733225989218 26.428878552601226 22.693174616326907 18-19 22.32730458832191 26.625087827832257 28.879190892218805 22.168416691627034 20-21 22.905050765186864 25.73829965316754 29.339394736768437 22.017254844877154 22-23 22.742215864715746 25.615970529038474 29.224343775631027 22.417469830614753 24-25 22.567875868130884 25.853350614304947 29.236352744567274 22.342420772996896 26-27 22.36291160111188 26.11866764828557 29.138797367544434 22.379623383058117 28-29 22.35361794683022 26.23721772624589 29.162227453790663 22.246936873133222 30-31 22.464945847668048 25.90684511229366 29.164774810837745 22.463434229200548 32-33 22.294160953775265 25.939008994129882 29.22798285712686 22.53884719496799 34-35 22.762062855335312 25.9993617610915 28.897806193716708 22.34076918985648 36-37 22.952722732782245 25.7499447139542 28.752578849098487 22.544753704165068 38-39 22.566000341513803 25.98320983783693 28.82759991378176 22.62318990686751 40-41 22.86955572413523 25.916810597005313 28.74471283448058 22.46892084437888 42-43 22.748458289127825 25.966498055890693 28.520097527384085 22.764946127597394 44-45 22.74092818972491 26.1249940515014 28.578322831317376 22.55575492745631 46-47 22.969882401681815 26.326935081585408 27.910551376832487 22.792631139900287 48-49 23.08582913672589 26.38927534690244 27.649881169992696 22.875014346378975 50-51 23.109679116990872 26.556281194626475 27.495192213485314 22.838847474897335 52-53 23.181480994197067 26.448228467134893 27.449367779572437 22.920922759095603 54-55 22.96596339084015 26.464268418651134 27.469214770192004 23.100553420316714 56-57 22.964703708783905 26.676902749745963 27.401835776649975 22.95655776482016 58-59 22.999302975928877 26.430620911281995 27.467283257705756 23.102792855083376 60-61 22.92198649060977 26.48352755764445 27.69299028925099 22.901495662494785 62-63 22.804332186556113 26.423034826009918 27.8063616743134 22.96627131312057 64-65 22.72273278224576 26.398317064772854 27.711073724991813 23.167876427989576 66-67 22.71954158770326 26.592084157958528 27.505633578084893 23.182740676253314 68-69 22.64723583767457 26.33480109620332 27.79653615427466 23.221426911847452 70-71 22.7805381921603 26.23800152841423 27.61668154957689 23.364778729848588 72-73 22.733146153910752 26.461833033342387 27.39447363485456 23.410547177892298 74-75 22.65196664361915 26.64373112226474 27.25867990919092 23.44562232492519 76-77 22.80144891429403 26.52434125626692 27.314105919665877 23.360103909773173 78-79 22.812954010407775 26.51289214602234 27.3081994104688 23.365954433101084 80-81 22.764862148793643 26.664669837333054 27.140325781772678 23.430142232100618 82-83 22.74929807716532 26.663382162342224 27.137078601361015 23.450241159131437 84-85 22.85401964544149 26.484087416336116 27.114124395002705 23.54776854321969 86-87 22.714726802954935 26.352912524878718 27.242444007132598 23.689916665033746 88-89 22.723740527890758 26.527224528528997 27.09906419619688 23.64997074738336 90-91 22.79923747246195 26.51980640086442 27.0953411358973 23.585614990776328 92-93 22.594027593763062 26.2939947580416 27.13336313648603 23.97861451170931 94-95 22.524881008133967 26.316944559294104 27.180617097709796 23.977557334862134 96-97 22.571732110538626 26.214697331715257 27.14998971206376 24.063580845682356 98-99 22.50665403603537 26.14481892176063 27.047270809468944 24.301256232735057 100-101 22.333590595675698 26.164643979991205 27.208306074442596 24.293459349890504 102-103 22.303619603341033 25.938955603388063 27.325837085236582 24.43158770803432 104-105 22.296744189171637 26.017622857194052 27.146081470299716 24.539551483334602 106-107 22.28547510590106 26.015600283336088 27.167731043499927 24.531193567262925 108-109 22.263310523679376 25.9015058627596 27.057453840060635 24.777729773500386 110-111 22.2200228849289 26.108500992361517 27.07454512214208 24.596931000567505 112-113 22.1991661743418 26.160874032966635 27.155640922976126 24.48431886971544 114-115 22.055484512983803 26.18427282662231 26.908272388700155 24.851970271693737 116-117 21.908878160561656 26.01153932953617 27.170092270956403 24.909490238945768 118-119 21.881098085064803 26.100685981200613 27.002327666191277 25.015888267543307 120-121 21.956251793676458 26.190070207121508 27.010184582432125 24.843493416769913 122-123 21.790617389300763 26.298573041575224 26.917045810547606 24.993763758576407 124-125 21.68635773158932 26.20747289912202 26.97158887296183 25.134580496326826 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 277.0 1 195.0 2 175.5 3 229.5 4 150.5 5 113.5 6 294.5 7 709.5 8 1165.0 9 1401.0 10 1227.0 11 965.5 12 913.5 13 982.0 14 1191.0 15 1383.5 16 1594.0 17 1780.5 18 1960.5 19 2224.5 20 2541.5 21 2906.0 22 3396.5 23 4123.5 24 5123.0 25 6583.0 26 8718.0 27 11176.5 28 13399.0 29 16111.5 30 19471.5 31 22839.5 32 26625.5 33 29484.5 34 31718.0 35 34406.5 36 38447.0 37 42390.5 38 44506.0 39 46974.0 40 49526.0 41 52475.5 42 54861.5 43 57155.0 44 60277.0 45 63044.0 46 64772.0 47 66787.0 48 67886.0 49 67278.5 50 66469.5 51 64515.5 52 63866.0 53 65381.5 54 66269.0 55 62354.5 56 55905.0 57 50728.0 58 47275.5 59 42797.0 60 36023.5 61 29930.5 62 24629.5 63 18971.0 64 14510.0 65 11237.5 66 8066.5 67 6494.5 68 4950.0 69 3319.5 70 2421.5 71 1756.0 72 1320.0 73 1038.0 74 781.5 75 576.5 76 386.5 77 194.0 78 97.0 79 50.0 80 23.0 81 14.5 82 7.0 83 2.5 84 2.0 85 1.0 86 0.5 87 0.5 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.16767767815403392 2 0.0023514065049981383 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 5.598586916662235E-5 10-11 3.919010841663564E-4 12-13 0.0050107352904127 14-15 2.7992934583311174E-5 16-17 5.038728224996011E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 2.7992934583311174E-5 94-95 7.558092337494016E-4 96-97 0.005178692897912567 98-99 0.0017355619441652924 100-101 0.0028272863929144285 102-103 0.004002989645413498 104-105 0.010581329272491623 106-107 0.0167957607499867 108-109 0.024885718844563632 110-111 0.015312135217071213 112-113 0.023514065049981384 114-115 0.025725506882062966 116-117 0.024969697648313565 118-119 0.014332382506655321 120-121 0.021134665610399936 122-123 0.013072700450406316 124-125 0.013716537945822473 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1786165.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 76.09669691449699 #Duplication Level Percentage of deduplicated Percentage of total 1 86.36760301240267 65.72289309666402 2 9.035308162820836 13.751142135905155 3 2.368507861102852 5.4070687453784165 4 0.8658323637900214 2.6354793186436702 5 0.4347527944777772 1.6541625817053003 6 0.24769863601430564 1.1309428818548957 7 0.14989940641924163 0.7984794788563624 8 0.09585218911129206 0.5835227986714433 9 0.07928621707744067 0.5430077307395117 >10 0.331179272428903 4.35511976994241 >50 0.01688724688528318 0.885930334070685 >100 0.006448751988052693 1.0053324640525123 >500 2.4802849376750303E-4 0.12975977517514609 >1k 2.4802849376750303E-4 0.43438854191359805 >5k 2.4802849376750303E-4 0.9627703464267929 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5133 0.2873754664322725 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5069 0.28379237080560865 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2799 0.15670444779737594 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1804 0.1009985079765867 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 5.598586916662234E-5 0.0 14-15 0.0 0.0 0.0 5.598586916662234E-5 0.0 16-17 0.0 0.0 0.0 5.598586916662234E-5 0.0 18-19 0.0 0.0 0.0 1.3996467291655585E-4 0.0 20-21 0.0 0.0 0.0 1.959505420831782E-4 0.0 22-23 0.0 0.0 0.0 3.079222804164229E-4 0.0 24-25 0.0 0.0 0.0 3.919010841663564E-4 0.0 26-27 0.0 0.0 0.0 6.43837495416157E-4 0.0 28-29 0.0 0.0 0.0 0.001763554878748604 0.0 30-31 0.0 0.0 0.0 0.005906509197078658 0.0 32-33 0.0 0.0 0.0 0.014948227067488166 0.0 34-35 0.0 0.0 0.0 0.03079222804164229 0.0 36-37 0.0 0.0 0.0 0.05816931806412061 0.0 38-39 0.0 0.0 0.0 0.09931893190158804 0.0 40-41 0.0 0.0 0.0 0.14170023486072114 0.0 42-43 0.0 0.0 0.0 0.185677135091103 0.0 44-45 0.0 0.0 0.0 0.24191494066897515 0.0 46-47 0.0 0.0 0.0 0.31256910755725253 0.0 48-49 0.0 0.0 0.0 0.391229253736357 0.0 50-51 0.0 0.0 0.0 0.47669168301920595 0.0 52-53 0.0 0.0 0.0 0.5622100981712216 0.0 54-55 0.0 0.0 0.0 0.6541948812119821 0.0 56-57 0.0 0.0 0.0 0.7704215456018901 2.799293458331117E-5 58-59 0.0 0.0 0.0 0.9038078788913678 5.598586916662234E-5 60-61 0.0 0.0 0.0 1.0479714919954204 5.598586916662234E-5 62-63 0.0 0.0 0.0 1.2000291126519667 5.598586916662234E-5 64-65 0.0 0.0 0.0 1.356313666430593 5.598586916662234E-5 66-67 0.0 0.0 0.0 1.541822843914196 5.598586916662234E-5 68-69 0.0 0.0 0.0 1.7609515358323558 5.598586916662234E-5 70-71 0.0 0.0 0.0 2.002390596613415 5.598586916662234E-5 72-73 0.0 0.0 0.0 2.2433817704411405 5.598586916662234E-5 74-75 0.0 0.0 0.0 2.4949262806067747 5.598586916662234E-5 76-77 0.0 0.0 0.0 2.7699848558223903 5.598586916662234E-5 78-79 0.0 0.0 0.0 3.0932752573250513 5.598586916662234E-5 80-81 5.598586916662234E-5 0.0 0.0 3.4586950253755955 5.598586916662234E-5 82-83 5.598586916662234E-5 0.0 0.0 3.836991543334462 5.598586916662234E-5 84-85 5.598586916662234E-5 0.0 0.0 4.21901112159291 5.598586916662234E-5 86-87 5.598586916662234E-5 0.0 0.0 4.60612541394552 8.397880374993351E-5 88-89 5.598586916662234E-5 0.0 0.0 5.046258324398922 1.1197173833324468E-4 90-91 5.598586916662234E-5 0.0 0.0 5.542349111084363 1.1197173833324468E-4 92-93 5.598586916662234E-5 0.0 0.0 6.0569712204639545 1.6795760749986703E-4 94-95 5.598586916662234E-5 0.0 0.0 6.574308644498129 1.6795760749986703E-4 96-97 5.598586916662234E-5 0.0 0.0 7.098868245654797 1.6795760749986703E-4 98-99 5.598586916662234E-5 0.0 0.0 7.656963382442271 1.6795760749986703E-4 100-101 5.598586916662234E-5 0.0 0.0 8.285124834491775 1.6795760749986703E-4 102-103 5.598586916662234E-5 0.0 0.0 8.94771759607875 1.6795760749986703E-4 104-105 1.1197173833324468E-4 0.0 0.0 9.603760010973229 1.6795760749986703E-4 106-107 1.1197173833324468E-4 0.0 0.0 10.2521043688573 1.6795760749986703E-4 108-109 1.1197173833324468E-4 0.0 0.0 10.943613831868838 1.6795760749986703E-4 110-111 1.1197173833324468E-4 0.0 0.0 11.678456357615339 1.6795760749986703E-4 112-113 1.1197173833324468E-4 0.0 0.0 12.460578949873051 1.6795760749986703E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3755 0.0 74.428116 1 GTATCAA 6355 0.0 49.978798 1 TGGTATC 1580 0.0 41.797134 2 ATCAACG 8175 0.0 38.425064 3 TCAACGC 8315 0.0 37.7781 4 GTGGTAT 1845 0.0 37.465866 1 TATCAAC 8380 0.0 37.415127 2 CAACGCA 8525 0.0 36.847496 5 AACGCAG 8855 0.0 35.743042 6 ACGCAGA 10555 0.0 29.760767 7 CGCAGAG 10510 0.0 29.718372 8 GTACATG 7195 0.0 29.318827 1 TACATGG 7100 0.0 28.825756 2 ACATGGG 7215 0.0 27.293554 3 GAGTACT 7485 0.0 26.707184 12-13 GCAGAGT 11485 0.0 26.57387 9 CAGAGTA 11120 0.0 25.761196 10-11 GTACTTT 8155 0.0 24.51297 14-15 AGAGTAC 10710 0.0 23.997656 10-11 AGTACTT 7950 0.0 22.338694 12-13 >>END_MODULE