FastQCFastQC Report
Thu 26 May 2016
SRR1364114_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364114_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1298070
Sequences flagged as poor quality0
Sequence length125
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN55840.43017710909273No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA28450.056.0599171
GTATCAA49750.048.6856841
GTACATG122500.041.779411
TACATGG124900.040.3126682
ATCAACG59000.039.446533
TCAACGC59700.038.8888244
CAACGCA61350.038.0384565
AACGCAG61700.037.5332226
ACATGGG126550.037.1106833
TATCAAC65850.036.42362
ATGGGAG36050.034.8440485
CATGGGA98650.034.3963474
CATGGGG25350.033.111264
ACGCAGA73650.031.3649087
CGCAGAG72300.031.2917868
ATGGGCG2451.8189894E-1129.1585925
ATGGGAT35600.028.5954865
GCAGAGT76200.027.65889
CATTCCG3300.027.0621579
GAGTACA84250.026.6300131
Produced by FastQC (version 0.11.5)