FastQCFastQC Report
Thu 26 May 2016
SRR1364114_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364114_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1298070
Sequences flagged as poor quality0
Sequence length125
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT126380.973599266603496No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT116850.9001825787515312No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70250.5411880715215667No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30430.23442495397012486No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23930.18435061283289805No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14560.11216652414738804No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13030.1003797946181639No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA55100.085.2540361
GTATCAA100750.053.8439181
GTGGTAT17350.048.4462281
TGGTATC17250.047.93972
ATCAACG134000.040.5355033
TCAACGC135450.040.1454934
TATCAAC135950.039.8665582
CAACGCA138100.039.3320585
AACGCAG141600.038.5699426
GTACATG106100.035.0036241
ACGCAGA162750.033.4505427
CGCAGAG163900.033.1795358
TACATGG110250.033.1328932
ACATGGG109700.031.9974213
CATGGGA86950.031.2691614
GCAGAGT171800.031.20369
ATGGGAG33150.030.3300235
GAGTACT120100.029.74904412-13
CAGAGTA171650.028.752510-11
ATGGGAT32350.027.769775
Produced by FastQC (version 0.11.5)