##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364114_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1298070 Sequences flagged as poor quality 0 Sequence length 125 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.178137542659485 33.0 31.0 34.0 30.0 34.0 2 32.17083131110033 33.0 31.0 34.0 30.0 34.0 3 32.30865361652299 34.0 31.0 34.0 30.0 34.0 4 35.944130902031475 37.0 35.0 37.0 35.0 37.0 5 35.89900929841996 37.0 35.0 37.0 35.0 37.0 6 35.95331530657052 37.0 35.0 37.0 35.0 37.0 7 35.88339303735546 37.0 35.0 37.0 35.0 37.0 8 35.87082591847897 37.0 35.0 37.0 35.0 37.0 9 37.520387190213164 39.0 37.0 39.0 34.0 39.0 10-11 37.45790789402729 39.0 37.0 39.0 34.0 39.0 12-13 37.40780658978329 39.0 37.0 39.0 34.0 39.0 14-15 38.65773301902054 40.0 38.0 41.0 34.0 41.0 16-17 38.6626842157973 40.0 38.0 41.0 34.0 41.0 18-19 38.870400286579304 40.0 38.0 41.0 34.5 41.0 20-21 38.94956088654695 40.0 38.0 41.0 35.0 41.0 22-23 38.89933208532668 40.0 38.0 41.0 35.0 41.0 24-25 38.85728234995031 40.0 38.0 41.0 35.0 41.0 26-27 38.721531581501765 40.0 38.0 41.0 34.5 41.0 28-29 38.62020461146163 40.0 38.0 41.0 34.0 41.0 30-31 38.38603311069511 40.0 37.5 41.0 34.0 41.0 32-33 38.10962698467725 40.0 37.0 41.0 33.5 41.0 34-35 37.91900398283606 40.0 36.5 41.0 33.0 41.0 36-37 37.73991502769496 40.0 36.0 41.0 33.0 41.0 38-39 37.59642315129385 40.0 36.0 41.0 33.0 41.0 40-41 37.40187509148197 40.0 36.0 41.0 33.0 41.0 42-43 37.164825086474536 40.0 36.0 41.0 32.5 41.0 44-45 36.83801027679554 40.0 36.0 41.0 31.0 41.0 46-47 36.308884343679466 40.0 35.0 41.0 30.0 41.0 48-49 35.800553899250424 40.0 35.0 41.0 27.5 41.0 50-51 35.543702573821136 40.0 35.0 41.0 26.0 41.0 52-53 35.3845231767162 40.0 35.0 41.0 26.0 41.0 54-55 35.22315707165254 39.0 35.0 41.0 25.0 41.0 56-57 35.03749258514564 39.0 35.0 41.0 25.0 41.0 58-59 34.844996802945914 39.0 35.0 41.0 24.0 41.0 60-61 34.63081497916137 39.0 35.0 41.0 23.5 41.0 62-63 34.397925766715204 38.0 34.5 40.5 23.0 41.0 64-65 34.19357006941074 38.0 34.0 40.0 23.0 41.0 66-67 33.98714283513216 37.0 34.0 40.0 22.5 41.0 68-69 33.67390356452272 37.0 34.0 40.0 22.0 41.0 70-71 33.35880499510812 36.0 34.0 39.0 22.0 41.0 72-73 33.02774503686241 36.0 34.0 39.0 21.0 41.0 74-75 32.74001248006656 35.0 34.0 38.5 20.5 40.0 76-77 32.514585114824314 35.0 34.0 37.0 20.0 39.5 78-79 32.205947290978145 35.0 33.5 37.0 20.0 39.0 80-81 31.914016963646027 35.0 33.0 36.5 20.0 39.0 82-83 31.63295084240449 35.0 33.0 36.0 18.5 37.5 84-85 31.40228878257721 35.0 33.0 36.0 18.0 37.0 86-87 31.193421001949048 35.0 33.0 35.5 17.5 37.0 88-89 31.022459497561766 35.0 33.0 35.0 15.0 36.0 90-91 30.874261018280986 35.0 33.0 35.0 12.0 36.0 92-93 30.747516312679593 35.0 33.0 35.0 10.0 36.0 94-95 30.648057885938357 35.0 33.0 35.0 7.0 36.0 96-97 30.567378877872535 35.0 33.0 35.0 7.0 35.5 98-99 30.49567396211298 35.0 33.0 35.0 2.0 35.0 100-101 30.425666566517982 35.0 33.0 35.0 2.0 35.0 102-103 29.280318472809633 33.5 30.0 34.5 2.0 35.0 104-105 30.15316700948331 35.0 32.0 35.0 2.0 35.0 106-107 30.272302341168043 35.0 33.0 35.0 2.0 35.0 108-109 30.248250094370874 35.0 33.0 35.0 2.0 35.0 110-111 30.194446370380643 35.0 33.0 35.0 2.0 35.0 112-113 30.119609882363818 35.0 33.0 35.0 2.0 35.0 114-115 30.035044335051268 35.0 33.0 35.0 2.0 35.0 116-117 29.952672043880533 35.0 33.0 35.0 2.0 35.0 118-119 29.851411326045593 35.0 32.0 35.0 2.0 35.0 120-121 29.713844784949963 35.0 32.0 35.0 2.0 35.0 122-123 29.5614716463673 35.0 32.0 35.0 2.0 35.0 124-125 28.725770952259893 34.5 30.5 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.11897230740920861 1101 2 0.15466302845690905 1101 3 0.06696982907871529 1101 4 0.06553626347455577 1101 5 0.1671564466409805 1101 6 0.2245037821141267 1101 7 0.23043143679944933 1101 8 0.2083988891467925 1101 9 0.24626467468143431 1101 10-11 0.252454729225434 1101 12-13 0.2130488702330453 1101 14-15 0.3879612956057841 1101 16-17 0.31960661483500985 1101 18-19 0.27533294688652177 1101 20-21 0.24137819666054838 1101 22-23 0.2657294114447737 1101 24-25 0.193403312909048 1101 26-27 0.27853468530528147 1101 28-29 0.32948373983121115 1101 30-31 0.3575972432821928 1101 32-33 0.3367057183249713 1101 34-35 0.3506473058215249 1101 36-37 0.29638999191327997 1101 38-39 0.27942578487635217 1101 40-41 0.33513954003772994 1101 42-43 0.2722443636877685 1101 44-45 0.26465063839216185 1101 46-47 0.33422066973735554 1101 48-49 0.23616342613399866 1101 50-51 0.08620248395094166 1101 52-53 0.07061204195399995 1101 54-55 0.14789387912967555 1101 56-57 0.07979071553901207 1101 58-59 0.09733999141119654 1101 60-61 0.12026852832818236 1101 62-63 0.1440590072484369 1101 64-65 0.20958710428264027 1101 66-67 0.22537422138746876 1101 68-69 0.21945595117305317 1101 70-71 0.21259648007868748 1101 72-73 0.19543370054854847 1101 74-75 0.12622900586507058 1101 76-77 0.04629705492343561 1101 78-79 -0.008667639354868584 1101 80-81 0.042219838291334355 1101 82-83 0.06763505233749356 1101 84-85 0.04438274363124606 1101 86-87 0.07821171973566976 1101 88-89 0.041661087402015795 1101 90-91 0.046068178945724014 1101 92-93 0.06816671425294629 1101 94-95 0.08794762689722546 1101 96-97 0.11533359239681573 1101 98-99 0.12242455084232873 1101 100-101 0.1159929930893604 1101 102-103 0.22134656790288076 1101 104-105 0.14052551974462446 1101 106-107 0.12411853677808082 1101 108-109 0.1267640126872891 1101 110-111 0.05870539017542953 1101 112-113 0.1749498927801838 1101 114-115 0.10326101820176703 1101 116-117 0.20087185622470827 1101 118-119 0.1894603139480644 1101 120-121 0.2145748259902014 1101 122-123 0.2948215494733155 1101 124-125 0.37883976946680775 1102 1 0.3172421914782504 1102 2 0.32410549921652176 1102 3 0.19264410050927694 1102 4 0.17119300289093786 1102 5 0.19049310636531658 1102 6 0.19783680528530567 1102 7 0.24653356416832395 1102 8 0.20736416462820983 1102 9 0.3168835924726565 1102 10-11 0.3266295187544017 1102 12-13 0.27768102036645104 1102 14-15 0.44932054789511966 1102 16-17 0.4579022286218759 1102 18-19 0.4470936834784567 1102 20-21 0.4211207993666761 1102 22-23 0.38797928652106606 1102 24-25 0.3630282002421552 1102 26-27 0.34190582014248605 1102 28-29 0.393676117321327 1102 30-31 0.46072076271183704 1102 32-33 0.4931368374440197 1102 34-35 0.3892061059415539 1102 36-37 0.5254056815053474 1102 38-39 0.5072053408517405 1102 40-41 0.487759050866849 1102 42-43 0.4999417659520873 1102 44-45 0.5075645863550022 1102 46-47 0.4562161333213197 1102 48-49 0.5485299447571279 1102 50-51 0.4419820858617314 1102 52-53 0.5298774935505932 1102 54-55 0.5736325190234624 1102 56-57 0.47026686254762495 1102 58-59 0.573041485460358 1102 60-61 0.5467974928115709 1102 62-63 0.6072155707263178 1102 64-65 0.5060847060715403 1102 66-67 0.3654349621312605 1102 68-69 0.2603645241042045 1102 70-71 0.33301070556795054 1102 72-73 0.23975709995722383 1102 74-75 0.19921889742511212 1102 76-77 0.18549400387005477 1102 78-79 0.2498016279429791 1102 80-81 0.2390462904906876 1102 82-83 0.3101127611499983 1102 84-85 0.2559182436043024 1102 86-87 0.22650019881135108 1102 88-89 0.318237150401842 1102 90-91 0.3134649634082507 1102 92-93 0.25856824566879055 1102 94-95 0.3220284314028987 1102 96-97 0.305413281496552 1102 98-99 0.3438988831078582 1102 100-101 0.3461837163158634 1102 102-103 0.4632821800054465 1102 104-105 0.49846482199166076 1102 106-107 0.41722648822671005 1102 108-109 0.39712377821647493 1102 110-111 0.4951008975747122 1102 112-113 0.38472630218010195 1102 114-115 0.35863965700017175 1102 116-117 0.35365685230694766 1102 118-119 0.34048327487970553 1102 120-121 0.3858894590973243 1102 122-123 0.39290925253505904 1102 124-125 0.4993273149347637 1103 1 0.16180287078923783 1103 2 0.1742186421115477 1103 3 0.0621921744630356 1103 4 0.04417142054204248 1103 5 0.04465711965517727 1103 6 0.13969892041481557 1103 7 0.14853948795927607 1103 8 0.21063341564027382 1103 9 0.1714529545049146 1103 10-11 0.16231425280898293 1103 12-13 0.16348622499777576 1103 14-15 0.2031568218969042 1103 16-17 0.22438122521799642 1103 18-19 0.23379722699412753 1103 20-21 0.20879729617651321 1103 22-23 0.24023664866033556 1103 24-25 0.28849201050870477 1103 26-27 0.29980029222082294 1103 28-29 0.32154948451320564 1103 30-31 0.25771969903486536 1103 32-33 0.31675241446696845 1103 34-35 0.34387538033240617 1103 36-37 0.288700916709395 1103 38-39 0.2256920931592319 1103 40-41 0.25002637516387693 1103 42-43 0.20438961059593197 1103 44-45 0.1290130596674075 1103 46-47 0.21332747395558727 1103 48-49 0.23394471771239722 1103 50-51 0.26809300021102445 1103 52-53 0.28299801007074166 1103 54-55 0.17508514468883618 1103 56-57 0.16524963958779182 1103 58-59 0.24475785532683147 1103 60-61 0.15539882447233566 1103 62-63 0.2540945889565407 1103 64-65 0.2671151932984017 1103 66-67 0.17501962796862358 1103 68-69 0.24463414324503674 1103 70-71 0.30042229505261275 1103 72-73 0.1435402773446981 1103 74-75 0.14173817766067032 1103 76-77 0.19914559121456676 1103 78-79 0.13045230281665 1103 80-81 0.1221479585850922 1103 82-83 0.05926818767632014 1103 84-85 0.08480015568724042 1103 86-87 -0.008659937204377854 1103 88-89 0.05905559429302798 1103 90-91 0.06374920185566424 1103 92-93 0.19213219742938037 1103 94-95 0.10995907082454437 1103 96-97 0.10659888295092301 1103 98-99 0.164717001324302 1103 100-101 0.13103687480838389 1103 102-103 0.2940252360092934 1103 104-105 0.18366558011593526 1103 106-107 0.1651331556610387 1103 108-109 0.19899097038260294 1103 110-111 0.20103550557572447 1103 112-113 0.2866039915410603 1103 114-115 0.3124506912093956 1103 116-117 0.31397062247479113 1103 118-119 0.2956410613847922 1103 120-121 0.31875757187616216 1103 122-123 0.23129214367769535 1103 124-125 0.21320635119341347 1104 1 0.18142500703292797 1104 2 0.2861207217385413 1104 3 0.16478019626011786 1104 4 0.13986671670070194 1104 5 0.14159366715831112 1104 6 0.1629802418334947 1104 7 0.17715963446261185 1104 8 0.17030421641305793 1104 9 0.19052285709089745 1104 10-11 0.20995630004959764 1104 12-13 0.2592635066148503 1104 14-15 0.18164562174873566 1104 16-17 0.3224256383975188 1104 18-19 0.2664907339157381 1104 20-21 0.2326491174431169 1104 22-23 0.28014615030769363 1104 24-25 0.2133627453532867 1104 26-27 0.11791873869408676 1104 28-29 0.25450789367247495 1104 30-31 0.13852771033101874 1104 32-33 0.14822302119334552 1104 34-35 0.13151095369537558 1104 36-37 0.1667830177120777 1104 38-39 0.12697310532706751 1104 40-41 0.16997458414268607 1104 42-43 0.1899856576224508 1104 44-45 0.25304388959813195 1104 46-47 0.21067717719371615 1104 48-49 0.1982075537465775 1104 50-51 0.16426032870848672 1104 52-53 0.1516844925802161 1104 54-55 0.03124781743177607 1104 56-57 -0.021812715238475278 1104 58-59 0.048209400646527456 1104 60-61 0.02040870520685445 1104 62-63 0.0821230808149167 1104 64-65 -0.03050179103006201 1104 66-67 -0.006766346655822986 1104 68-69 0.011251703595675622 1104 70-71 0.048219732348606215 1104 72-73 0.09863178670973127 1104 74-75 0.026688366184743018 1104 76-77 -0.043923026790963604 1104 78-79 -0.010405511459524064 1104 80-81 -0.04230297672880923 1104 82-83 -0.04898405226835223 1104 84-85 -0.01434830001257481 1104 86-87 -0.010416889826124986 1104 88-89 0.02341443402462673 1104 90-91 0.11599645300643502 1104 92-93 0.09464044270269056 1104 94-95 0.059515924553991795 1104 96-97 0.14914423832602353 1104 98-99 0.08834648482440954 1104 100-101 0.08306476175851074 1104 102-103 0.18709156012117134 1104 104-105 0.15246607763598163 1104 106-107 0.17998696666798963 1104 108-109 0.1714697688580955 1104 110-111 0.19637210473394973 1104 112-113 0.2415247332673296 1104 114-115 0.24004818593768462 1104 116-117 0.2246553242431304 1104 118-119 0.27251181661954504 1104 120-121 0.22042982035072 1104 122-123 0.23975193851864063 1104 124-125 0.3493897199546616 1105 1 0.08288036271363808 1105 2 0.19810177104353244 1105 3 0.138084215338786 1105 4 0.07613071634498425 1105 5 0.11284037432051264 1105 6 0.0767791978859691 1105 7 0.12700736952162117 1105 8 0.1535127770864051 1105 9 0.174915700638671 1105 10-11 0.14070679594094315 1105 12-13 0.15210447868117427 1105 14-15 0.24610254888172278 1105 16-17 0.22575678644697916 1105 18-19 0.2636289880962437 1105 20-21 0.22053231483336333 1105 22-23 0.2935009189441544 1105 24-25 0.3066322065804954 1105 26-27 0.2535862317559463 1105 28-29 0.15980633946387712 1105 30-31 0.22205950865154733 1105 32-33 0.29741555066756575 1105 34-35 0.31943384408305064 1105 36-37 0.3344302152888545 1105 38-39 0.5008477925820287 1105 40-41 0.6449732827329342 1105 42-43 0.5958503522195358 1105 44-45 0.6156028190584664 1105 46-47 0.6917535948898532 1105 48-49 0.6375436937869452 1105 50-51 0.6072172346935645 1105 52-53 0.7096355910242806 1105 54-55 0.8841332055284283 1105 56-57 0.7646323159248638 1105 58-59 0.7145344472532642 1105 60-61 0.6634089644042973 1105 62-63 0.6420925844463952 1105 64-65 0.7385351223730439 1105 66-67 0.750964433962281 1105 68-69 0.6725702539987424 1105 70-71 0.5912661644795421 1105 72-73 0.6210708021064875 1105 74-75 0.5829118431828704 1105 76-77 0.5542500379313893 1105 78-79 0.5067031464942033 1105 80-81 0.5292463624261607 1105 82-83 0.5121746145241133 1105 84-85 0.5249176723519895 1105 86-87 0.5820907044420309 1105 88-89 0.5056801504452686 1105 90-91 0.4966283037087109 1105 92-93 0.46643490780701313 1105 94-95 0.5305317670487071 1105 96-97 0.5076236549040338 1105 98-99 0.47199706389752194 1105 100-101 0.41554187592576497 1105 102-103 0.33056832507680767 1105 104-105 0.3975922981143185 1105 106-107 0.45939462459677216 1105 108-109 0.4725656914328944 1105 110-111 0.4411529093739084 1105 112-113 0.49913906482532155 1105 114-115 0.45432706394895916 1105 116-117 0.32726009586198757 1105 118-119 0.3596654653929079 1105 120-121 0.35872616111184996 1105 122-123 0.36500544229327403 1105 124-125 0.34313156580918047 1106 1 0.09165034399399019 1106 2 0.20144132988102115 1106 3 0.03081504064222429 1106 4 0.06518468413339207 1106 5 0.08375914658783046 1106 6 0.13479715839356032 1106 7 0.13474539800653673 1106 8 0.10262682889755581 1106 9 0.07594743776999735 1106 10-11 0.11351316838424452 1106 12-13 0.1597589373211079 1106 14-15 0.10648753089532903 1106 16-17 0.19743307892202466 1106 18-19 0.08854423209862716 1106 20-21 0.07545883806901088 1106 22-23 0.15278547480546933 1106 24-25 0.10191897515564818 1106 26-27 0.21989464438178175 1106 28-29 0.2727900740762408 1106 30-31 0.2324345701158208 1106 32-33 0.20861207674997928 1106 34-35 0.16668434226716045 1106 36-37 0.21054304212781716 1106 38-39 0.18811595365725253 1106 40-41 0.22212873774108743 1106 42-43 0.17047768462695245 1106 44-45 0.03858754572576828 1106 46-47 0.13643305331412137 1106 48-49 0.20321719125504245 1106 50-51 0.17190999959379383 1106 52-53 0.28742671601457204 1106 54-55 0.18543915958334622 1106 56-57 0.22735908674292205 1106 58-59 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-0.23911895001453587 2216 78-79 -0.17950981885604733 2216 80-81 -0.2550185840121557 2216 82-83 -0.41029697489064887 2216 84-85 -0.2975693090939302 2216 86-87 -0.3915629455388725 2216 88-89 -0.36182324054422565 2216 90-91 -0.4147361244018839 2216 92-93 -0.3488459514908513 2216 94-95 -0.3890926199647282 2216 96-97 -0.46595842428775214 2216 98-99 -0.47943838313103626 2216 100-101 -0.5233897015375426 2216 102-103 -0.6604908234377511 2216 104-105 -0.5488026898609313 2216 106-107 -0.7430037944648546 2216 108-109 -0.647578273640562 2216 110-111 -0.6703468349545361 2216 112-113 -0.6229009872230264 2216 114-115 -0.7764153277743553 2216 116-117 -0.7849245291803726 2216 118-119 -0.7863429325337812 2216 120-121 -0.8877003654370306 2216 122-123 -0.9878688362861077 2216 124-125 -1.484058176975747 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5.0 3 11.0 4 33.0 5 126.0 6 346.0 7 838.0 8 1584.0 9 2914.0 10 5100.0 11 7574.0 12 9623.0 13 14311.0 14 22525.0 15 21293.0 16 8061.0 17 5991.0 18 5435.0 19 4770.0 20 4534.0 21 4520.0 22 4573.0 23 4957.0 24 5747.0 25 7255.0 26 8832.0 27 11225.0 28 14381.0 29 18266.0 30 22819.0 31 29069.0 32 37032.0 33 48423.0 34 65884.0 35 98660.0 36 176170.0 37 391746.0 38 232071.0 39 1366.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.396553240808615 19.286434115107003 14.063746680021175 21.253265964063207 2 15.958772035921548 21.525656542000277 39.99604021721797 22.51953120486021 3 18.45200952182856 27.871070127188823 31.41294383199673 22.263976518985878 4 11.80213701880484 17.806281633502046 37.877541272812714 32.5140400748804 5 11.356321307787717 39.95169752016455 34.305391850978765 14.386589321068971 6 28.735045105425748 38.42859013766592 17.72593157533877 15.11043318156956 7 25.828961458164812 35.837589652329996 21.337909357738795 16.99553953176639 8 26.448188464412553 34.68225904612232 19.901083916892002 18.968468572573126 9 27.788023758348935 14.810449359433619 20.487647045228684 36.91387983698876 10-11 24.26957428442335 27.75877722280493 27.261266126450867 20.710382366320857 12-13 25.977169743071972 25.9257818263204 28.505116256494272 19.591932174113357 14-15 22.653824524101164 25.97818299475375 28.512406880984848 22.85558560016024 16-17 19.993567335933363 29.658972507101932 28.826154364678125 21.52130579228658 18-19 19.7852581139692 26.299352115063137 33.99231166269924 19.92307810826843 20-21 21.21430277257775 25.019105287080052 34.422450253068014 19.34414168727418 22-23 20.69191183834462 25.50397898418421 34.087799579375535 19.716309598095634 24-25 20.1281903133113 25.660480559600025 34.56281248314806 19.64851664394062 26-27 20.02184011648062 25.863243122481837 34.41582503254832 19.69909172848922 28-29 19.780905498162657 25.70808970240434 34.587580022649014 19.923424776783992 30-31 19.800588566101982 25.41168812159591 34.87897416934372 19.908749142958392 32-33 19.828090935003505 25.51877017418167 34.50819293258453 20.144945958230295 34-35 20.20711517868836 25.628394462548243 34.248538214426034 19.91595214433736 36-37 20.469735838591138 25.267435500396747 34.01480659748704 20.248022063525077 38-39 20.223485636367837 25.49716117004476 33.99882132704708 20.280531866540326 40-41 20.12745845755622 25.591532043726456 33.92270832851849 20.358301170198835 42-43 20.410609597325262 25.58151717549901 33.45066136649025 20.557211860685477 44-45 20.38584205782431 25.884197308311567 33.07429491475806 20.655665719106057 46-47 20.819139183557127 26.172163288574573 31.989338017210166 21.01935951065813 48-49 21.054064880938625 26.407358617023736 31.138651998736588 21.399924503301055 50-51 21.150169097198148 26.808608164428726 30.378754612617193 21.66246812575593 52-53 21.20216937453296 26.917924303003687 30.22190636868582 21.657999953777534 54-55 20.85380603511367 27.040529401341995 30.32355728119439 21.782107282349948 56-57 21.054796736693707 27.079471831257173 30.09849237714453 21.76723905490459 58-59 21.095433990462766 26.617439737456376 30.281918540602586 22.005207731478272 60-61 21.085573197131126 26.71928324358471 30.51410940858351 21.681034150700658 62-63 21.11943115548468 26.60199372915174 30.50571232676204 21.77286278860154 64-65 21.010192054357624 26.579537313087894 30.514340520927224 21.895930111627262 66-67 21.12370673384332 26.791582888442072 30.188510635019682 21.896199742694925 68-69 21.270463072099346 26.557196453195896 30.30283420770837 21.869506266996385 70-71 21.08095095025692 26.736539631915075 30.236504965063517 21.94600445276449 72-73 21.3210766753719 26.77802429761107 29.96302202500636 21.937877002010676 74-75 20.876801713312844 26.959062300184115 29.781290685402173 22.382845301100865 76-77 20.824031061498996 27.067531026832143 29.797391512013988 22.31104639965487 78-79 20.889705485836664 26.819971187994483 29.735530441347542 22.55479288482131 80-81 20.531673946705496 27.004360319551335 29.743811966997157 22.720153766746016 82-83 20.643070096373847 27.030707126734306 29.49517360388885 22.831049173002995 84-85 20.55050960271788 26.510742872110132 29.614350535795452 23.324396989376535 86-87 20.57500751115117 26.491213879066617 29.46393491876401 23.469843691018205 88-89 20.59361205481985 26.40142673353517 29.566317687027666 23.438643524617316 90-91 20.433720831696288 26.64629026169621 29.471022363971127 23.448966542636377 92-93 20.478256364547505 26.410951031512564 29.545066731789017 23.565725872150914 94-95 20.295887481404957 26.220570527887364 29.45481760872563 24.028724381982048 96-97 20.45367872917259 26.189705767922533 29.42640500035054 23.930210502554335 98-99 20.27086264038495 26.18632890562526 29.21474563918776 24.32806281480203 100-101 20.23974931097111 26.12909252343469 29.392094512999584 24.23906365259462 102-103 20.450473666835773 25.934246345275064 29.354901709268372 24.2603782786208 104-105 20.241448599138927 25.820237981502654 29.383295991420095 24.555017427938324 106-107 20.11723370074008 26.041022163834388 29.096803522789877 24.74494061263566 108-109 20.254475023897488 26.092204814481896 28.814919584613293 24.83840057700732 110-111 20.17853345373741 26.104864036885562 28.779090373940942 24.93751213543609 112-113 20.026484320157444 25.959488313996005 28.86744663259228 25.146580733254275 114-115 19.65722656513385 26.067988303998934 28.838178990119896 25.436606140747326 116-117 19.60984589872376 26.09114615039631 28.82016740154641 25.47884054933352 118-119 19.60626966686905 26.14795957678285 28.60618337149849 25.639587384849616 120-121 19.43541722451354 26.336280049084102 28.68368685574706 25.544615870655296 122-123 19.619985198831472 26.284016779794285 28.41277307712903 25.683224944245207 124-125 19.402339358814434 26.237386131351432 28.40423245037858 25.95604205945556 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1612.0 1 1082.0 2 745.5 3 983.5 4 698.5 5 503.5 6 1150.5 7 2650.5 8 4345.0 9 4971.0 10 3737.0 11 2294.0 12 2009.0 13 2137.0 14 2364.5 15 2595.5 16 2824.0 17 3057.5 18 3297.5 19 3515.5 20 3869.0 21 4337.0 22 4873.0 23 5646.0 24 6662.5 25 8043.5 26 10345.0 27 12445.0 28 13914.0 29 15921.0 30 18714.0 31 22156.5 32 25753.5 33 28501.5 34 31587.5 35 34716.5 36 37320.0 37 40070.0 38 40707.5 39 40358.5 40 40842.0 41 42139.0 42 43113.5 43 44267.5 44 45508.0 45 46567.0 46 46781.5 47 46232.5 48 45908.5 49 44786.5 50 43372.0 51 40995.5 52 38790.5 53 37145.5 54 34947.0 55 31674.5 56 27611.0 57 24054.0 58 21900.0 59 19859.5 60 17360.5 61 14957.5 62 12423.5 63 8963.5 64 6215.5 65 4875.5 66 3532.0 67 2699.0 68 2067.0 69 1401.5 70 1013.0 71 860.5 72 753.5 73 811.5 74 683.0 75 502.0 76 356.0 77 196.0 78 100.0 79 45.5 80 23.0 81 11.5 82 8.0 83 2.0 84 1.5 85 1.0 86 0.5 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.16393568913849021 2 0.0014637115101650913 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 6.548183071791198E-4 12-13 0.007665226066390873 14-15 0.0 16-17 5.777808592756939E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 3.851872395171293E-5 94-95 6.933370311308326E-4 96-97 0.006240033280177495 98-99 0.0024651983329096274 100-101 0.003659278775412728 102-103 0.004506690702350413 104-105 0.011863766977127582 106-107 0.018103800257305076 108-109 0.026154213563213076 110-111 0.016794163642946836 112-113 0.0247290207769997 114-115 0.027849037417088447 116-117 0.027117181662005902 118-119 0.015561564476492023 120-121 0.022918640751269193 122-123 0.01536897085673346 124-125 0.01486822744536119 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1298070.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.74518056109463 #Duplication Level Percentage of deduplicated Percentage of total 1 77.41980717701546 40.061019013797946 2 11.792532242850726 12.204134203576821 3 4.320108609478958 6.706344001230844 4 2.068958757058269 4.282345778297522 5 1.152278601569762 2.981243214745647 6 0.7319556622198248 2.2725106742568255 7 0.48483550045124757 1.7561530359294937 8 0.35891267620687584 1.4857600988792388 9 0.24927523191622855 1.1608912696422586 >10 1.2741552171563195 11.833675109827983 >50 0.0909791810348649 3.2392658196859485 >100 0.050914896913867934 4.90568378130774 >500 0.002782234804030738 1.083785400018834 >1k 0.0016693408824184426 1.5141034872473538 >5k 2.782234804030738E-4 1.0113698771429742 >10k+ 5.564469608061476E-4 3.5017152344126066 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12638 0.973599266603496 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11685 0.9001825787515312 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7025 0.5411880715215667 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3043 0.23442495397012486 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2393 0.18435061283289805 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1456 0.11216652414738804 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1303 0.1003797946181639 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 3.0814979161370343E-4 0.0 0.0 0.0 0.0 3 3.0814979161370343E-4 0.0 0.0 0.0 0.0 4 3.0814979161370343E-4 0.0 0.0 0.0 0.0 5 3.0814979161370343E-4 0.0 0.0 0.0 0.0 6 4.622246874205551E-4 0.0 0.0 0.0 0.0 7 7.703744790342585E-4 0.0 0.0 0.0 0.0 8 7.703744790342585E-4 0.0 0.0 0.0 0.0 9 7.703744790342585E-4 0.0 0.0 0.0 0.0 10-11 7.703744790342585E-4 0.0 0.0 0.0 0.0 12-13 9.629680987928231E-4 0.0 0.0 0.0 0.0 14-15 0.0012325991664548137 0.0 0.0 3.851872395171293E-5 0.0 16-17 0.0013481553383099523 0.0 0.0 1.1555617185513879E-4 0.0 18-19 0.0018874174736339335 0.0 0.0 2.696310676619905E-4 0.0 20-21 0.00223408598919935 0.0 0.0 5.007434113722681E-4 0.0 22-23 0.0024266796089579146 0.0 0.0 0.001078524270647962 0.0 24-25 0.003004460468233608 0.0 0.0 0.0024651983329096274 0.0 26-27 0.003004460468233608 0.0 0.0 0.0041600221867849965 0.0 28-29 0.0038133536712195797 0.0 0.0 0.009051900128652538 0.0 30-31 0.004237059634688422 0.0 0.0 0.02168604158481438 0.0 32-33 0.004237059634688422 0.0 0.0 0.04533653809116612 0.0 34-35 0.0045837281502538385 0.0 0.0 0.07992635219980432 0.0 36-37 0.0053155839053363844 0.0 0.0 0.13928370580939395 0.0 38-39 0.005700771144853513 0.0 0.0 0.230380487955195 0.0 40-41 0.0057392898688052255 0.0 0.0 0.340544038457094 0.0 42-43 0.0063170707280809195 0.0 0.0 0.43637862364895574 0.0 44-45 0.006933370311308327 0.0 0.0 0.5482369980047301 0.0 46-47 0.007857819686149438 0.0 0.0 0.6859029174081521 0.0 48-49 0.00855115671728027 0.0 0.0 0.8482978575885738 0.0 50-51 0.00855115671728027 0.0 0.0 1.0333803261765544 0.0 52-53 0.008628194165183695 0.0 0.0 1.2089101512245102 0.0 54-55 0.0089363439567974 0.0 0.0 1.383785157965287 0.0 56-57 0.009475606092121381 0.0 0.0 1.590823299205744 0.0 58-59 0.009475606092121381 0.0 0.0 1.8363031269500105 0.0 60-61 0.009475606092121381 0.0 0.0 2.120378716093893 0.0 62-63 0.009668199711879946 0.0 0.0 2.399331314952198 0.0 64-65 0.009899312055590224 0.0 0.0 2.67439352269138 0.0 66-67 0.010245980571155638 0.0 0.0 2.990131502923571 0.0 68-69 0.010400055466962491 0.0 0.0 3.35709938601154 0.0 70-71 0.010554130362769342 0.0 0.0 3.7805357184127204 0.0 72-73 0.011093392498093324 0.0 0.0 4.2125617262551325 0.0 74-75 0.011478579737610453 0.0 0.0 4.619781675872641 0.0 76-77 0.011478579737610453 0.0 0.0 5.060243284260479 0.0 78-79 0.011478579737610453 0.0 0.0 5.584097930003775 0.0 80-81 0.013211922315437535 0.0 0.0 6.160646190113014 0.0 82-83 0.013481553383099525 0.0 0.0 6.772978344773394 0.0 84-85 0.01394377807052008 0.0 0.0 7.345597695039558 0.0 86-87 0.014444521481892347 3.851872395171293E-5 0.0 7.93050451824632 0.0 88-89 0.014483040205844061 7.703744790342586E-5 0.0 8.579814647900344 0.0 90-91 0.015985270439960862 7.703744790342586E-5 0.0 9.299845154729715 0.0 92-93 0.016486013851333133 7.703744790342586E-5 0.0 10.069372221837035 0.0 94-95 0.016486013851333133 7.703744790342586E-5 0.0 10.822875499780444 0.0 96-97 0.016486013851333133 7.703744790342586E-5 0.0 11.565092791605998 0.0 98-99 0.017140832158512254 7.703744790342586E-5 0.0 12.350759204049089 0.0 100-101 0.0178726879135948 7.703744790342586E-5 0.0 13.23522614342832 0.0 102-103 0.018643062392629056 7.703744790342586E-5 0.0 14.158674031446687 0.0 104-105 0.021069742001586973 7.703744790342586E-5 0.0 15.070990008243008 0.0 106-107 0.021570485412959237 7.703744790342586E-5 0.0 15.948523577310931 0.0 108-109 0.022032710100379792 7.703744790342586E-5 0.0 16.84643355134931 0.0 110-111 0.022417897339896922 7.703744790342586E-5 0.0 17.83832921182987 0.0 112-113 0.022533453511752063 7.703744790342586E-5 0.0 18.89840301370496 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5510 0.0 85.254036 1 GTATCAA 10075 0.0 53.843918 1 GTGGTAT 1735 0.0 48.446228 1 TGGTATC 1725 0.0 47.9397 2 ATCAACG 13400 0.0 40.535503 3 TCAACGC 13545 0.0 40.145493 4 TATCAAC 13595 0.0 39.866558 2 CAACGCA 13810 0.0 39.332058 5 AACGCAG 14160 0.0 38.569942 6 GTACATG 10610 0.0 35.003624 1 ACGCAGA 16275 0.0 33.450542 7 CGCAGAG 16390 0.0 33.179535 8 TACATGG 11025 0.0 33.132893 2 ACATGGG 10970 0.0 31.997421 3 CATGGGA 8695 0.0 31.269161 4 GCAGAGT 17180 0.0 31.2036 9 ATGGGAG 3315 0.0 30.330023 5 GAGTACT 12010 0.0 29.749044 12-13 CAGAGTA 17165 0.0 28.7525 10-11 ATGGGAT 3235 0.0 27.76977 5 >>END_MODULE