FastQCFastQC Report
Thu 26 May 2016
SRR1364113_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364113_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1205891
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN49720.41230923856302104No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA24750.061.5585371
GTATCAA42150.049.4190061
GTATACG705.4442302E-542.510431
ATCAACG50950.039.8371243
TCAACGC52400.038.7460634
CAACGCA53200.038.165015
GTACATG103500.038.0088421
AACGCAG54100.036.979816
TATCAAC56700.036.738942
TACATGG105150.036.6215442
ACATGGG108250.033.761233
CATGGGA82750.033.0974354
GGACCGA2950.032.2939076
ATGGGAG32150.031.4840475
TGGTATC12450.030.5951252
ACGCAGA66950.029.7931777
CGCAGAG66400.029.7709458
GTGGTAT13350.029.4229471
ATGGGAT28000.029.3456485
GAGTACA74400.028.317431
Produced by FastQC (version 0.11.5)