##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364113_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1205891 Sequences flagged as poor quality 0 Sequence length 125 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.754317761721417 31.0 31.0 34.0 27.0 34.0 2 30.72562777232768 31.0 31.0 34.0 27.0 34.0 3 30.837941405981137 31.0 31.0 34.0 27.0 34.0 4 34.18801533471931 37.0 35.0 37.0 30.0 37.0 5 34.3098903632252 37.0 35.0 37.0 30.0 37.0 6 34.29479198368675 37.0 35.0 37.0 30.0 37.0 7 34.180380316297246 37.0 35.0 37.0 30.0 37.0 8 34.07748295658563 37.0 35.0 37.0 30.0 37.0 9 35.38453973037364 39.0 35.0 39.0 28.0 39.0 10-11 35.30903041817212 39.0 35.0 39.0 27.5 39.0 12-13 35.154586525647844 39.0 35.0 39.0 27.0 39.0 14-15 36.34569210650051 40.0 36.0 41.0 27.0 41.0 16-17 36.41079790793695 40.0 36.0 41.0 27.0 41.0 18-19 36.45198405162655 40.0 36.5 41.0 27.0 41.0 20-21 36.51198035311649 40.0 37.0 41.0 27.5 41.0 22-23 36.52915644946351 40.0 36.5 41.0 28.0 41.0 24-25 36.59527643874944 40.0 36.0 41.0 29.0 41.0 26-27 36.617011404845044 40.0 36.0 41.0 30.0 41.0 28-29 36.61611497224874 40.0 36.0 41.0 30.0 41.0 30-31 36.5803414238932 40.0 36.0 41.0 30.0 41.0 32-33 36.50327185458719 40.0 36.0 41.0 30.0 41.0 34-35 36.47531742089459 40.0 36.0 41.0 30.0 41.0 36-37 36.40652928000955 40.0 36.0 41.0 30.0 41.0 38-39 36.30298882734841 40.0 36.0 41.0 29.5 41.0 40-41 36.19890645174398 40.0 35.5 41.0 29.0 41.0 42-43 36.05450534086414 40.0 35.0 41.0 29.0 41.0 44-45 35.92915860554561 40.0 35.0 41.0 28.0 41.0 46-47 35.78311140890843 40.0 35.0 41.0 28.0 41.0 48-49 35.64424023398466 40.0 35.0 41.0 27.0 41.0 50-51 35.503993727459616 39.0 35.0 41.0 27.0 41.0 52-53 35.34300571112978 39.0 35.0 41.0 27.0 41.0 54-55 35.15315563346936 39.0 34.5 41.0 26.5 41.0 56-57 34.95529736933106 39.0 34.0 41.0 26.0 41.0 58-59 34.731215756647984 38.0 34.0 41.0 26.0 41.0 60-61 34.479388269752405 38.0 34.0 40.0 26.0 41.0 62-63 34.204587728078245 37.5 34.0 40.0 25.5 41.0 64-65 33.91045583721912 37.0 33.0 40.0 25.0 41.0 66-67 33.59700793852844 36.5 33.0 40.0 24.0 41.0 68-69 33.2623520699632 36.0 33.0 39.0 23.0 41.0 70-71 32.90012488690935 35.5 33.0 39.0 23.0 41.0 72-73 32.551101633563896 35.0 32.5 38.5 22.0 40.0 74-75 32.2728509458981 35.0 32.0 37.0 21.5 40.0 76-77 32.03366805125837 35.0 32.0 37.0 21.0 39.0 78-79 31.720061763459547 35.0 32.0 36.5 20.0 39.0 80-81 31.500930017721338 35.0 32.0 36.0 20.0 38.5 82-83 31.38217964973617 35.0 32.0 36.0 20.0 37.0 84-85 31.156413805227835 35.0 32.0 35.5 18.5 37.0 86-87 30.950823084341785 35.0 32.0 35.0 17.5 36.5 88-89 30.756873962903775 35.0 32.0 35.0 13.5 36.0 90-91 30.593778376320913 35.0 32.0 35.0 8.5 36.0 92-93 30.438812463149652 35.0 32.0 35.0 6.0 36.0 94-95 30.30867922556848 35.0 32.0 35.0 2.0 35.5 96-97 30.196905856333615 35.0 32.0 35.0 2.0 35.0 98-99 30.092808139375784 35.0 32.0 35.0 2.0 35.0 100-101 29.98354619115658 35.0 32.0 35.0 2.0 35.0 102-103 28.890216031133825 33.5 29.5 34.5 2.0 35.0 104-105 29.663042513792703 35.0 31.0 35.0 2.0 35.0 106-107 29.7192955250516 35.0 31.0 35.0 2.0 35.0 108-109 29.659079469039902 35.0 31.0 35.0 2.0 35.0 110-111 29.550995902614744 35.0 31.0 35.0 2.0 35.0 112-113 29.42938084785441 35.0 31.0 35.0 2.0 35.0 114-115 29.281126154851478 35.0 31.0 35.0 2.0 35.0 116-117 29.14134237671564 35.0 31.0 35.0 2.0 35.0 118-119 28.961192595350656 35.0 30.5 35.0 2.0 35.0 120-121 28.769611847173586 35.0 30.0 35.0 2.0 35.0 122-123 28.5527265731314 35.0 30.0 35.0 2.0 35.0 124-125 27.688798987636527 34.0 27.5 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2990564689209485 1101 2 0.2880881024404687 1101 3 0.26638564166207956 1101 4 0.3672806737354364 1101 5 0.31182345625347097 1101 6 0.2617676144538734 1101 7 0.2476007165180647 1101 8 0.32307929555746284 1101 9 0.39340215407661105 1101 10-11 0.41436929420069646 1101 12-13 0.34228535656788495 1101 14-15 0.4280140517856381 1101 16-17 0.3525388306047148 1101 18-19 0.36789464611693745 1101 20-21 0.30683585532791113 1101 22-23 0.35131064233678444 1101 24-25 0.3115229067640968 1101 26-27 0.18776440133760275 1101 28-29 0.3122627873000994 1101 30-31 0.33389432183151513 1101 32-33 0.3159253525026813 1101 34-35 0.2788650050806183 1101 36-37 0.3204257776957746 1101 38-39 0.3244160633887887 1101 40-41 0.43564274098638833 1101 42-43 0.4175304288766313 1101 44-45 0.35794988382287585 1101 46-47 0.42631285095961147 1101 48-49 0.35490385981167805 1101 50-51 0.4281835563016543 1101 52-53 0.39639082209793486 1101 54-55 0.3098338980296731 1101 56-57 0.2527533088493996 1101 58-59 0.2643360377823356 1101 60-61 0.2576803721579495 1101 62-63 0.3239748435016807 1101 64-65 0.42991728896414827 1101 66-67 0.4868085844093457 1101 68-69 0.49594573954939847 1101 70-71 0.4240200959747611 1101 72-73 0.44325447337722323 1101 74-75 0.4610506018724223 1101 76-77 0.45947865282904843 1101 78-79 0.3995971759473349 1101 80-81 0.31322735649752786 1101 82-83 0.3050749206038539 1101 84-85 0.3661610259326018 1101 86-87 0.4256012440038184 1101 88-89 0.4315984238604038 1101 90-91 0.41027229022004974 1101 92-93 0.49062028286734716 1101 94-95 0.5256262809685239 1101 96-97 0.42296301162327765 1101 98-99 0.31649905798555267 1101 100-101 0.30136552791011795 1101 102-103 0.2642305849237303 1101 104-105 0.3782869697121498 1101 106-107 0.4018512918934256 1101 108-109 0.4294932193659555 1101 110-111 0.3948547521657204 1101 112-113 0.3857368350460817 1101 114-115 0.5026419931991271 1101 116-117 0.4656634046596331 1101 118-119 0.47311101087962726 1101 120-121 0.6326575874868077 1101 122-123 0.6406166258329975 1101 124-125 0.4915035011794622 1102 1 0.42378707605382715 1102 2 0.41020342505735385 1102 3 0.28981183045885217 1102 4 0.3691622717846741 1102 5 0.3422572789065086 1102 6 0.4155144955536798 1102 7 0.32666365594024427 1102 8 0.20215975487840154 1102 9 0.44490218957231065 1102 10-11 0.5296950047498257 1102 12-13 0.4102158331807715 1102 14-15 0.48540670007138687 1102 16-17 0.5858903676990295 1102 18-19 0.42794800690897006 1102 20-21 0.5647027132154818 1102 22-23 0.5259096222748667 1102 24-25 0.609555435159038 1102 26-27 0.5897011642725332 1102 28-29 0.6717468490769605 1102 30-31 0.6337281758770814 1102 32-33 0.7082862532990148 1102 34-35 0.7455692190396164 1102 36-37 0.600867090268359 1102 38-39 0.6827982928708067 1102 40-41 0.6651066841880535 1102 42-43 0.5832255227188128 1102 44-45 0.6720709222253234 1102 46-47 0.800341685195967 1102 48-49 0.846127953645393 1102 50-51 0.7844220405503606 1102 52-53 0.759238509047961 1102 54-55 0.6623311674655241 1102 56-57 0.8327710501892582 1102 58-59 0.888454099755883 1102 60-61 0.7568636452788411 1102 62-63 0.7611837542915296 1102 64-65 0.6744426462495952 1102 66-67 0.7741512065455112 1102 68-69 0.6711613352552703 1102 70-71 0.7098634316071681 1102 72-73 0.5548311535452797 1102 74-75 0.5020401635297631 1102 76-77 0.6916197537951163 1102 78-79 0.55523623867834 1102 80-81 0.5911965071471634 1102 82-83 0.5656977432353969 1102 84-85 0.6397021687119633 1102 86-87 0.5221914092835469 1102 88-89 0.6096590492230973 1102 90-91 0.58255655936998 1102 92-93 0.5656515288577992 1102 94-95 0.6276207488488161 1102 96-97 0.7240006259992349 1102 98-99 0.7297618818675851 1102 100-101 0.7017898155699207 1102 102-103 0.7908181710659434 1102 104-105 0.6828417526119459 1102 106-107 0.6657846589639647 1102 108-109 0.6225533855640109 1102 110-111 0.7376817213469806 1102 112-113 0.7163169716491886 1102 114-115 0.7243589172259419 1102 116-117 0.6952253681127338 1102 118-119 0.6981062401265277 1102 120-121 0.8373126376139091 1102 122-123 0.808189247115962 1102 124-125 0.8282602162179842 1103 1 0.34985604238003276 1103 2 0.4652071835334759 1103 3 0.36854530709325317 1103 4 0.30049408217761453 1103 5 0.34771953236953834 1103 6 0.38956488161613834 1103 7 0.44525359690919686 1103 8 0.32388843884481133 1103 9 0.4666211963180231 1103 10-11 0.4590659560792716 1103 12-13 0.5196891480003103 1103 14-15 0.548317920857663 1103 16-17 0.4739718440323557 1103 18-19 0.6173923096163421 1103 20-21 0.5848152035749195 1103 22-23 0.5647411809623222 1103 24-25 0.5895485144799864 1103 26-27 0.5049282221726159 1103 28-29 0.5457425930548396 1103 30-31 0.6068518947450272 1103 32-33 0.25125604570796867 1103 34-35 0.5511582538688131 1103 36-37 0.6062433239918121 1103 38-39 0.5097852266678657 1103 40-41 0.6100115297386282 1103 42-43 0.6764112930988944 1103 44-45 0.624063598817493 1103 46-47 0.6971839787559233 1103 48-49 0.6005499058829358 1103 50-51 0.6199180860860807 1103 52-53 0.6672638575012186 1103 54-55 0.7931911400369529 1103 56-57 0.8639957269065448 1103 58-59 0.836823194818443 1103 60-61 0.802389592449039 1103 62-63 0.8068157598906325 1103 64-65 0.6983268089402941 1103 66-67 0.7406929490903877 1103 68-69 0.7817876078342252 1103 70-71 0.7646042433793596 1103 72-73 0.7334555415099544 1103 74-75 0.810608609338793 1103 76-77 0.8280739419300644 1103 78-79 0.8006964980474081 1103 80-81 0.7773226961501258 1103 82-83 0.7643036369560541 1103 84-85 0.6890862967015146 1103 86-87 0.7650823176813226 1103 88-89 0.7915614735135073 1103 90-91 0.8223018822910184 1103 92-93 0.7401694517547881 1103 94-95 0.6800820375222649 1103 96-97 0.6853103156341511 1103 98-99 0.6605140365154369 1103 100-101 0.7708055053749199 1103 102-103 0.7891525161087145 1103 104-105 0.7440995371539998 1103 106-107 0.8872835333761202 1103 108-109 0.8649814491130527 1103 110-111 0.8198266626531705 1103 112-113 0.8078204696223459 1103 114-115 0.7007924410734461 1103 116-117 0.826441638355476 1103 118-119 0.8405603050968438 1103 120-121 0.9095599326352222 1103 122-123 0.902176565069361 1103 124-125 0.8895468369227402 1104 1 0.164746059300505 1104 2 0.16008955594238117 1104 3 0.10975062282556536 1104 4 0.17825461319149127 1104 5 0.1534799515125087 1104 6 0.09925150325476295 1104 7 0.11868565631264971 1104 8 0.14907430768550256 1104 9 0.18430472286172517 1104 10-11 0.1016230451984228 1104 12-13 0.29131356009541776 1104 14-15 0.3584552890014976 1104 16-17 0.29340381953263517 1104 18-19 0.30275213770805465 1104 20-21 0.23870761173589727 1104 22-23 0.23924114134138108 1104 24-25 0.26605982043291476 1104 26-27 0.30975358703489775 1104 28-29 0.31289776265579405 1104 30-31 0.34816162331824785 1104 32-33 -0.24880967844635649 1104 34-35 0.2489404132568822 1104 36-37 0.43919443174679174 1104 38-39 0.5064873657327169 1104 40-41 0.5467224320240618 1104 42-43 0.5735487964200559 1104 44-45 0.5650549614516791 1104 46-47 0.6142275105202586 1104 48-49 0.548431117631246 1104 50-51 0.49360605017552217 1104 52-53 0.5046147545660062 1104 54-55 0.586865306715282 1104 56-57 0.5075493993019364 1104 58-59 0.4790418744621334 1104 60-61 0.5009049723672803 1104 62-63 0.5018510225308788 1104 64-65 0.5103617872420045 1104 66-67 0.41208895809599255 1104 68-69 0.5258614799191719 1104 70-71 0.40882261537807807 1104 72-73 0.4137303710233624 1104 74-75 0.4112322896117604 1104 76-77 0.4303892034515968 1104 78-79 0.42432829362197566 1104 80-81 0.4190221083617658 1104 82-83 0.4033046298102967 1104 84-85 0.48301683419458286 1104 86-87 0.560643839684932 1104 88-89 0.5653207553023236 1104 90-91 0.5559102315892623 1104 92-93 0.6145994928207301 1104 94-95 0.4706112334967756 1104 96-97 0.49579355131350766 1104 98-99 0.4762892319399157 1104 100-101 0.5512276315746192 1104 102-103 0.6181424617114892 1104 104-105 0.4956351983343339 1104 106-107 0.4781455452810448 1104 108-109 0.4959142193628523 1104 110-111 0.4797125543341707 1104 112-113 0.44798165694551173 1104 114-115 0.4356169202910962 1104 116-117 0.4157724173032378 1104 118-119 0.41085671637156906 1104 120-121 0.33962057598612816 1104 122-123 0.48520248648937425 1104 124-125 0.5813303853744927 1105 1 0.1662441866413289 1105 2 0.08517080768348961 1105 3 0.15752355129307816 1105 4 0.04058166305684807 1105 5 0.11583331146546527 1105 6 0.1041792278964806 1105 7 0.02533591786587408 1105 8 -0.030632876516058616 1105 9 -0.08126311073471015 1105 10-11 0.12645744641177714 1105 12-13 0.11547550642283966 1105 14-15 0.0986362095450346 1105 16-17 0.005176809906238589 1105 18-19 0.09280692459633855 1105 20-21 0.013777256085425904 1105 22-23 0.02941509744995585 1105 24-25 0.09318784222386256 1105 26-27 0.15687185861690267 1105 28-29 0.1932975212220498 1105 30-31 0.21157822034056295 1105 32-33 -0.09533752070964141 1105 34-35 0.22849670136781697 1105 36-37 0.2602982173041042 1105 38-39 0.2252828280164394 1105 40-41 0.22921848551091983 1105 42-43 0.22175816281124128 1105 44-45 0.21024331072583635 1105 46-47 0.2768594478713027 1105 48-49 0.047216984471248225 1105 50-51 0.1749970799833065 1105 52-53 0.13849905208666513 1105 54-55 0.03738763933668565 1105 56-57 0.11320452048310159 1105 58-59 0.052752680169881216 1105 60-61 0.05031144774413576 1105 62-63 0.16585031061085687 1105 64-65 0.062029382499993346 1105 66-67 0.0742682071751446 1105 68-69 0.07836945032806142 1105 70-71 0.154843601542062 1105 72-73 0.16872696618849403 1105 74-75 0.2140853864638359 1105 76-77 0.21475017785343198 1105 78-79 0.2912294295987081 1105 80-81 0.2511227367085631 1105 82-83 0.203234985460778 1105 84-85 0.16712786276424296 1105 86-87 0.19588233289073997 1105 88-89 0.12890914172012558 1105 90-91 0.16523251468580114 1105 92-93 0.2899438454378114 1105 94-95 0.26377215837374024 1105 96-97 0.3413053598998843 1105 98-99 0.23973987731088897 1105 100-101 0.19158885360080902 1105 102-103 0.2459050209091842 1105 104-105 0.3462565806972897 1105 106-107 0.3827343340884255 1105 108-109 0.3352663721470748 1105 110-111 0.28285970510644987 1105 112-113 0.30645273132567397 1105 114-115 0.27824622663337806 1105 116-117 0.3024842754144821 1105 118-119 0.2315797794848251 1105 120-121 0.23648503260543663 1105 122-123 0.2313612137001968 1105 124-125 0.42302877891149393 1106 1 0.1014072405036508 1106 2 0.2702195296937582 1106 3 0.28650343998131333 1106 4 0.2927983450293965 1106 5 0.18316552716128598 1106 6 0.11988984196022301 1106 7 0.14859397502563354 1106 8 0.17974636007999578 1106 9 0.21125693586090932 1106 10-11 0.22094532600277716 1106 12-13 0.2038820717214307 1106 14-15 0.07385100550428092 1106 16-17 0.11763158564939857 1106 18-19 0.15746759628827078 1106 20-21 0.1973943645681615 1106 22-23 0.22448403555051044 1106 24-25 0.24622903867125245 1106 26-27 0.2712853762782501 1106 28-29 0.25520020663379483 1106 30-31 0.34171930153159025 1106 32-33 0.2757582468546289 1106 34-35 0.3062810363632238 1106 36-37 0.27600944771206315 1106 38-39 0.3272592898308275 1106 40-41 0.3831812706471638 1106 42-43 0.30574060932796954 1106 44-45 0.3290627650146618 1106 46-47 0.3155224123637126 1106 48-49 0.39275819632724307 1106 50-51 0.25027859520392326 1106 52-53 0.277555552360937 1106 54-55 0.31607245323155553 1106 56-57 0.36991866619920444 1106 58-59 0.46265378774042887 1106 60-61 0.5913595102660878 1106 62-63 0.5588786870985629 1106 64-65 0.6495476416973958 1106 66-67 0.5780252874293268 1106 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2216 94-95 -2.3560302681090697 2216 96-97 -2.39384061686269 2216 98-99 -2.4683151592273695 2216 100-101 -2.520420926867466 2216 102-103 -2.691291102157507 2216 104-105 -2.627162025359347 2216 106-107 -2.5657925521267515 2216 108-109 -2.5813218963272817 2216 110-111 -2.5749637881625524 2216 112-113 -2.5756741176209843 2216 114-115 -2.6119165209630566 2216 116-117 -2.624669366576061 2216 118-119 -2.6606104511267326 2216 120-121 -2.677145676654785 2216 122-123 -2.735142193347585 2216 124-125 -3.008044353003953 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 26959.0 3 5366.0 4 3210.0 5 2816.0 6 3370.0 7 3594.0 8 3874.0 9 4265.0 10 4737.0 11 5209.0 12 5687.0 13 5883.0 14 6268.0 15 5720.0 16 4962.0 17 4678.0 18 4403.0 19 4381.0 20 4366.0 21 4669.0 22 5710.0 23 7341.0 24 9289.0 25 11361.0 26 13675.0 27 14893.0 28 17131.0 29 22001.0 30 25258.0 31 28866.0 32 37215.0 33 49603.0 34 67459.0 35 99640.0 36 180863.0 37 339352.0 38 160807.0 39 1010.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.022140737907954 18.769803479496865 12.56466463539102 22.643391147204163 2 15.923490466300263 20.744367548347483 39.60423919934751 23.727902786004744 3 19.23604319291692 26.63085584422292 28.85473124388736 25.278369718972794 4 12.25257556332508 16.369894794892108 36.8514441231672 34.52608551861561 5 13.02857647483219 40.34055833304869 32.94938112491388 13.681484067205243 6 31.443543035868505 36.10574823024932 17.975932057579 14.47477667630318 7 28.918939645270296 34.13519005996629 20.020355529918962 16.925514764844447 8 27.23408441240685 35.19164895882918 19.51002156618702 18.064245062576955 9 28.148270422948535 16.308262552897745 19.22201546665066 36.32145155750306 10-11 25.017052039990993 28.085168477490473 26.53118146872681 20.366598013791723 12-13 25.35454923369499 25.965808665790778 26.881697116364865 21.797944984149368 14-15 22.737830825462886 27.264545315084575 24.71607204791633 25.2815518115362 16-17 22.17278956764884 30.664331248158085 25.35770127457327 21.805177909619804 18-19 22.14877604135183 29.310640242161174 27.234502547803363 21.306081168683637 20-21 23.53740623116181 28.181595335508387 26.92254779428011 21.358450639049693 22-23 23.170505444528434 28.601441840045062 26.524361643269046 21.70369107215746 24-25 22.341910864989238 28.743562473761074 26.906014305660186 22.008512355589502 26-27 22.184984806689727 28.978862786563177 26.7525675193377 22.083584887409398 28-29 22.13623039547533 28.798600712982758 26.988048034628687 22.077120856913233 30-31 22.172799074198366 28.61155601208278 26.997698002239467 22.217946911479387 32-33 21.939481662069817 28.798487875321417 26.88807142404525 22.373959038563516 34-35 22.41821771479835 28.800095714204723 26.79939369887044 21.98229287212649 36-37 22.523134741903363 28.508043515397468 26.56205860662397 22.4067631360752 38-39 22.21332263717058 28.63497720192881 26.793387872100883 22.358312288799727 40-41 22.334971466985547 28.559504486436975 26.91170780327235 22.193816243305122 42-43 22.146397953827826 28.580717993151655 26.67738629831124 22.595497754709278 44-45 22.141737413729825 28.708289643663093 26.90442179038706 22.24555115222002 46-47 22.333839645853416 28.86893726862865 26.477381328316312 22.319841757201623 48-49 22.14827823992767 28.87240288081533 26.492542754941116 22.48677612431589 50-51 22.17574040986294 28.967977401007555 26.49436920658157 22.361912982547935 52-53 22.34635614762874 28.845501725923512 26.513317897364413 22.294824229083332 54-55 22.067930482492955 28.76125860627587 26.711622643049854 22.459188268181325 56-57 22.08530829373001 29.04231930075469 26.404656526604647 22.467715878910653 58-59 22.131881964962382 28.732478380056957 26.427753504532266 22.707886150448395 60-61 21.936319889236046 28.83465838824949 26.76258267622078 22.466439046293686 62-63 21.907243230002248 28.950965398784454 26.737174154216348 22.40461721699695 64-65 21.643774553301757 28.93988060273539 26.613092039210294 22.80325280475256 66-67 21.736275566917705 29.104164201565553 26.343445386413972 22.816114845102767 68-69 21.969447283584927 28.828704203321443 26.591938359563382 22.609910153530254 70-71 21.714639992766816 28.82011992255158 26.642914373831534 22.82232571085007 72-73 21.819425872132392 28.862449417217167 26.55552865493031 22.76259605572013 74-75 21.62195945622188 29.156262252486748 26.43603832129942 22.785739969991955 76-77 21.73675730471626 28.931122109914796 26.592471268414652 22.739649316954292 78-79 21.926238730292074 28.97621297810314 26.517253966587162 22.58029432501763 80-81 21.76363039394934 29.192034679942203 26.49424750958748 22.550087416520974 82-83 21.82810322873124 29.063081261625236 26.454529090581815 22.654286419061716 84-85 21.83609915283757 28.73493965863504 26.59620035233157 22.83276083619582 86-87 21.8539122913805 28.776378058388254 26.55901923263603 22.810690417595218 88-89 21.82804945727985 28.676131146120298 26.646159461229637 22.84965993537022 90-91 21.947457108162208 28.725336021645354 26.54080478563975 22.78640208455269 92-93 21.894415242170584 28.41506220792519 26.70370898038657 22.986813569517658 94-95 21.80535034330557 28.29968454127269 26.839026905842672 23.055938209579065 96-97 22.009949618808978 28.18172882124007 26.68112238097777 23.127199178973186 98-99 21.835449494224015 28.06747565842478 26.65467543525464 23.44239941209657 100-101 21.639887019198742 28.294412912852902 26.816577734255297 23.24912233369306 102-103 21.62810138238872 28.113413658363562 26.9486285601526 23.309856399095118 104-105 21.44919137342869 28.256925345569933 26.893963048725755 23.39992023227562 106-107 21.4007312318951 28.529867517300357 26.61256949492376 23.45683175588079 108-109 21.429068102432826 28.60084831895527 26.437247755852024 23.532835822759882 110-111 21.390081023507438 28.844368647588347 26.56642324214805 23.199127086756167 112-113 21.476426773171852 28.96571829572265 26.61241454562113 22.945440385484364 114-115 21.285057441587067 29.307306972988485 26.37332388293218 23.034311702492268 116-117 21.216547828791843 29.329434579343904 26.460752270189978 22.993265321674272 118-119 21.142799296295387 29.486189817779163 26.238992117691808 23.132018768233646 120-121 21.19912438009902 29.52663621575574 26.33137339440178 22.942866009743458 122-123 21.238704787920682 29.600826164083514 26.284093842912228 22.876375205083573 124-125 21.283873125262627 29.676999404048722 26.14939261695075 22.8897348537379 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 6578.0 1 3870.5 2 1383.0 3 1740.0 4 1702.0 5 1428.5 6 1229.0 7 1110.0 8 1097.5 9 1092.5 10 970.0 11 834.5 12 830.5 13 862.0 14 877.5 15 924.5 16 967.0 17 1041.0 18 1241.5 19 1460.5 20 1740.0 21 2266.0 22 2907.0 23 3742.5 24 4855.5 25 6099.5 26 7736.0 27 9564.5 28 11295.0 29 13356.5 30 15806.5 31 18405.0 32 21807.0 33 24181.5 34 25689.5 35 27203.0 36 29396.0 37 32521.5 38 33447.5 39 33457.0 40 33998.0 41 35268.5 42 36320.5 43 37308.0 44 38762.5 45 40194.5 46 40866.0 47 41109.5 48 41244.0 49 41450.5 50 41450.5 51 39870.5 52 38757.0 53 38987.0 54 38753.5 55 36478.5 56 33918.5 57 31719.5 58 29691.5 59 27277.0 60 23528.0 61 20312.0 62 17183.0 63 12546.5 64 8798.0 65 6739.5 66 4989.5 67 3991.0 68 2989.5 69 1862.0 70 1360.0 71 1012.0 72 849.5 73 804.5 74 614.5 75 472.0 76 353.5 77 207.0 78 118.5 79 75.0 80 57.5 81 42.5 82 27.0 83 16.5 84 13.5 85 13.0 86 12.0 87 7.5 88 4.5 89 4.0 90 2.5 91 3.5 92 5.5 93 5.5 94 4.5 95 3.5 96 3.0 97 1.5 98 1.0 99 1.0 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4693624879860618 2 0.4618991268696756 3 0.4568406265574584 4 0.4539382083455304 5 0.4617332744004226 6 0.5213572370968853 7 0.5155524006730293 8 0.5247572127165723 9 0.5313083852520667 10-11 0.5490545994621404 12-13 0.557886243449864 14-15 0.5362839593296574 16-17 0.5300230286153558 18-19 0.541052217820682 20-21 0.5416741645803808 22-23 0.5522472594952612 24-25 0.5452814557866341 26-27 0.5538643210704782 28-29 0.5326352050060909 30-31 0.5388546726030794 32-33 0.5405961235302361 34-35 0.5379424840221877 36-37 0.48561602997285824 38-39 0.4528187041780725 40-41 0.4510772532509157 42-43 0.46446984014309756 44-45 0.4697356560418811 46-47 0.47350879971738735 48-49 0.4878965014250874 50-51 0.46940395110337496 52-53 0.4696941929245678 54-55 0.45870646683655486 56-57 0.4427846297882645 58-59 0.4604479177637117 60-61 0.45613575356313296 62-63 0.4716429594382909 64-65 0.4723478324326162 66-67 0.4813867920069061 68-69 0.4892233211791115 70-71 0.4864867554364366 72-73 0.4976817971110158 74-75 0.48756479648658124 76-77 0.48607212426330404 78-79 0.4969769241166905 80-81 0.48835259571553313 82-83 0.49050867781582247 84-85 0.4880208907770271 86-87 0.47844291067766487 88-89 0.4830453166994364 90-91 0.46716494276845916 92-93 0.4678698157627845 94-95 0.46235522116012145 96-97 0.4513674950721085 98-99 0.41575067730002135 100-101 0.41288972220540665 102-103 0.4025239428770926 104-105 0.40667025460841816 106-107 0.40667025460841816 108-109 0.4184043168080697 110-111 0.4406285476879751 112-113 0.44145781003424023 114-115 0.4342432276217337 116-117 0.4283554649632512 118-119 0.4703161396842666 120-121 0.4642625245565312 122-123 0.4279822969074319 124-125 0.4386383180569388 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1205891.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 70.86254236845608 #Duplication Level Percentage of deduplicated Percentage of total 1 83.46042714680073 59.14218054779609 2 10.153504472204219 14.390062816997592 3 3.032325374383889 6.446348559516683 4 1.2354728860760629 3.501949989385748 5 0.6499421480661413 2.3028276502191147 6 0.37021974483477316 1.5740827412395864 7 0.23060445789241227 1.1438852717429138 8 0.1647795274628542 0.9341356997032544 9 0.11893938249806066 0.7585512328392144 >10 0.5488216379758671 6.763672168785229 >50 0.025297049684342668 1.2347124515180052 >100 0.009049182411824265 0.9032853520043707 >500 4.1132647256085514E-4 0.17969519806687045 >1k 2.0566323628042757E-4 0.724610320185304 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 4972 0.41230923856302104 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 8.292623462651267E-5 0.0 0.0 0.0 0.0 14-15 8.292623462651267E-5 0.0 0.0 1.6585246925302535E-4 0.0 16-17 8.292623462651267E-5 0.0 0.0 2.4877870387953805E-4 0.0 18-19 2.0731558656628167E-4 0.0 0.0 2.902418211927944E-4 0.0 20-21 3.317049385060507E-4 0.0 0.0 4.146311731325634E-4 0.0 22-23 3.317049385060507E-4 0.0 0.0 6.634098770121014E-4 0.0 24-25 4.146311731325634E-4 0.0 0.0 0.0010780410501446647 0.0 26-27 4.146311731325634E-4 0.0 0.0 0.0019487665137230478 0.0 28-29 4.975574077590761E-4 0.0 0.0 0.004685332256397967 0.0 30-31 4.975574077590761E-4 0.0 0.0 0.014346238590386695 0.0 32-33 4.975574077590761E-4 0.0 0.0 0.03590705959327999 0.0 34-35 4.975574077590761E-4 0.0 0.0 0.06658976640508968 0.0 36-37 4.975574077590761E-4 0.0 0.0 0.12040889267769642 0.0 38-39 4.975574077590761E-4 0.0 0.0 0.20652778733732982 0.0 40-41 4.975574077590761E-4 0.0 0.0 0.29716616178410815 0.0 42-43 5.390205250723325E-4 0.0 0.0 0.37669242079093385 0.0 44-45 5.804836423855888E-4 0.0 0.0 0.47798681638721907 0.0 46-47 5.804836423855888E-4 0.0 0.0 0.6094248982702417 0.0 48-49 6.634098770121014E-4 0.0 0.0 0.7522238742970966 0.0 50-51 6.634098770121014E-4 0.0 0.0 0.9218080241083149 0.0 52-53 6.634098770121014E-4 0.0 0.0 1.07161426696111 0.0 54-55 6.634098770121014E-4 0.0 0.0 1.2283033872879057 0.0 56-57 6.634098770121014E-4 0.0 0.0 1.424382469062295 0.0 58-59 6.634098770121014E-4 0.0 0.0 1.651144257648494 0.0 60-61 6.634098770121014E-4 0.0 0.0 1.9100814252697798 0.0 62-63 6.634098770121014E-4 0.0 0.0 2.1666551952042097 0.0 64-65 6.634098770121014E-4 0.0 0.0 2.414148542447037 0.0 66-67 6.634098770121014E-4 0.0 0.0 2.71392688062188 0.0 68-69 6.634098770121014E-4 0.0 0.0 3.061263414355029 0.0 70-71 6.634098770121014E-4 0.0 0.0 3.449689897345614 0.0 72-73 6.634098770121014E-4 0.0 0.0 3.841930987129019 0.0 74-75 6.634098770121014E-4 0.0 0.0 4.212072235384458 0.0 76-77 6.634098770121014E-4 0.0 0.0 4.616586407892587 0.0 78-79 6.634098770121014E-4 0.0 0.0 5.090468375665794 0.0 80-81 7.463361116386142E-4 0.0 0.0 5.622854801968005 0.0 82-83 7.463361116386142E-4 0.0 0.0 6.1756410819883385 0.0 84-85 7.463361116386142E-4 0.0 0.0 6.709271401809948 0.0 86-87 7.463361116386142E-4 0.0 0.0 7.2473797383013885 0.0 88-89 7.463361116386142E-4 0.0 0.0 7.852492472371052 0.0 90-91 7.877992289518705E-4 0.0 0.0 8.535390014520384 0.0 92-93 8.292623462651268E-4 0.0 0.0 9.247519054375562 0.0 94-95 8.292623462651268E-4 0.0 0.0 9.955418856264787 0.0 96-97 8.292623462651268E-4 0.0 0.0 10.635704222023385 0.0 98-99 8.707254635783831E-4 0.0 0.0 11.366906295842659 0.0 100-101 9.536516982048958E-4 0.0 0.0 12.199568617727472 0.0 102-103 0.0011195041674579211 0.0 0.0 13.072491626523458 0.0 104-105 0.0011609672847711775 0.0 0.0 13.934924466639192 0.0 106-107 0.0012438935193976903 0.0 0.0 14.744699147767086 0.0 108-109 0.0012438935193976903 0.0 0.0 15.580263887863829 0.0 110-111 0.0012438935193976903 0.0 0.0 16.500952407804682 0.0 112-113 0.0012438935193976903 0.0 0.0 17.471438131638763 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2475 0.0 61.558537 1 GTATCAA 4215 0.0 49.419006 1 GTATACG 70 5.4442302E-5 42.51043 1 ATCAACG 5095 0.0 39.837124 3 TCAACGC 5240 0.0 38.746063 4 CAACGCA 5320 0.0 38.16501 5 GTACATG 10350 0.0 38.008842 1 AACGCAG 5410 0.0 36.97981 6 TATCAAC 5670 0.0 36.73894 2 TACATGG 10515 0.0 36.621544 2 ACATGGG 10825 0.0 33.76123 3 CATGGGA 8275 0.0 33.097435 4 GGACCGA 295 0.0 32.293907 6 ATGGGAG 3215 0.0 31.484047 5 TGGTATC 1245 0.0 30.595125 2 ACGCAGA 6695 0.0 29.793177 7 CGCAGAG 6640 0.0 29.770945 8 GTGGTAT 1335 0.0 29.422947 1 ATGGGAT 2800 0.0 29.345648 5 GAGTACA 7440 0.0 28.31743 1 >>END_MODULE