FastQCFastQC Report
Thu 26 May 2016
SRR1364113_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364113_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1205891
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT113700.9428712877034491No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110940.9199836469465317No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64840.5376937053183082No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32060.2658615082125997No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22100.18326697852459303No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13060.10830166242222555No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA51950.081.998751
GTATCAA95300.052.8263361
TGGTATC17650.048.8782122
GTGGTAT18950.048.731431
ATCAACG125650.040.009973
TCAACGC127600.039.3519064
TATCAAC128500.039.1705132
CAACGCA130150.038.535185
AACGCAG132950.038.08166
GTACATG94550.035.3498881
TACATGG95500.034.4519422
ACATGGG94800.033.449753
ACGCAGA153950.032.853777
CGCAGAG154450.032.670368
GCAGAGT161150.031.0905069
CATGGGA74250.030.2879094
GAGTACT112300.029.80497712-13
CAGAGTA158450.028.50332810-11
GTACTTT117800.028.1814214-15
ATGGGAT26500.028.0632615
Produced by FastQC (version 0.11.5)