##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364113_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1205891 Sequences flagged as poor quality 0 Sequence length 125 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.10703289103244 33.0 31.0 34.0 30.0 34.0 2 32.10639601755051 33.0 31.0 34.0 30.0 34.0 3 32.23229462696048 34.0 31.0 34.0 30.0 34.0 4 35.890147616990255 37.0 35.0 37.0 35.0 37.0 5 35.83794721081756 37.0 35.0 37.0 35.0 37.0 6 35.894353635610514 37.0 35.0 37.0 35.0 37.0 7 35.822674686186396 37.0 35.0 37.0 33.0 37.0 8 35.80864854286167 37.0 35.0 37.0 33.0 37.0 9 37.442511802476346 39.0 37.0 39.0 34.0 39.0 10-11 37.373423053990784 39.0 37.0 39.0 34.0 39.0 12-13 37.314729938278006 39.0 37.0 39.0 34.0 39.0 14-15 38.55087690346806 40.0 38.0 41.0 34.0 41.0 16-17 38.54642293540627 40.0 38.0 41.0 33.5 41.0 18-19 38.75751829974682 40.0 38.0 41.0 34.0 41.0 20-21 38.83867115684585 40.0 38.0 41.0 34.0 41.0 22-23 38.79320187313779 40.0 38.0 41.0 34.0 41.0 24-25 38.75018969376171 40.0 38.0 41.0 34.0 41.0 26-27 38.60664438162321 40.0 38.0 41.0 34.0 41.0 28-29 38.50191435212635 40.0 38.0 41.0 34.0 41.0 30-31 38.25875472990511 40.0 37.5 41.0 34.0 41.0 32-33 37.97587219740424 40.0 36.5 41.0 33.0 41.0 34-35 37.781665175376546 40.0 36.0 41.0 33.0 41.0 36-37 37.597969468218935 40.0 36.0 41.0 33.0 41.0 38-39 37.44012103913206 40.0 36.0 41.0 33.0 41.0 40-41 37.23524141070793 40.0 35.5 41.0 32.5 41.0 42-43 36.98297856108056 40.0 35.0 41.0 31.5 41.0 44-45 36.65109740432593 40.0 35.0 41.0 31.0 41.0 46-47 36.10603528842988 40.0 35.0 41.0 29.0 41.0 48-49 35.57512329057933 40.0 35.0 41.0 26.5 41.0 50-51 35.30265546388521 39.0 35.0 41.0 24.5 41.0 52-53 35.12630702111551 39.0 35.0 41.0 24.0 41.0 54-55 34.944516544198436 39.0 35.0 41.0 24.0 41.0 56-57 34.73412107727813 39.0 35.0 41.0 23.5 41.0 58-59 34.51457718815382 38.0 34.5 41.0 23.0 41.0 60-61 34.27216597520008 38.0 34.0 40.0 23.0 41.0 62-63 34.0196099813333 37.5 34.0 40.0 22.0 41.0 64-65 33.79563492886173 37.0 34.0 40.0 22.0 41.0 66-67 33.57606284481765 36.5 34.0 40.0 22.0 41.0 68-69 33.259155263618354 36.0 34.0 39.0 21.5 41.0 70-71 32.938870096882724 35.5 33.5 39.0 20.0 41.0 72-73 32.61665233424911 35.0 33.0 38.5 20.0 40.5 74-75 32.346185932227705 35.0 33.0 37.0 20.0 40.0 76-77 32.15096803939991 35.0 33.0 37.0 20.0 39.0 78-79 31.875120554013588 35.0 33.0 36.5 19.0 39.0 80-81 31.61472305540053 35.0 33.0 36.0 18.0 38.5 82-83 31.356037568901336 35.0 33.0 36.0 17.5 37.0 84-85 31.142499612319853 35.0 33.0 35.0 16.0 37.0 86-87 30.95959916775231 35.0 33.0 35.0 13.0 36.5 88-89 30.8094583175428 35.0 33.0 35.0 10.5 36.0 90-91 30.6668745350948 35.0 33.0 35.0 8.5 36.0 92-93 30.548710870219615 35.0 33.0 35.0 7.0 36.0 94-95 30.451573566765155 35.0 32.0 35.0 6.0 35.5 96-97 30.37778290077627 35.0 32.0 35.0 2.0 35.0 98-99 30.30621714566242 35.0 32.0 35.0 2.0 35.0 100-101 30.225607870031368 35.0 32.0 35.0 2.0 35.0 102-103 29.062180163878825 33.5 30.0 34.5 2.0 35.0 104-105 29.944048840235144 35.0 31.5 35.0 2.0 35.0 106-107 30.067747416640476 35.0 32.0 35.0 2.0 35.0 108-109 30.046960712037823 35.0 32.0 35.0 2.0 35.0 110-111 29.982777464961593 35.0 32.0 35.0 2.0 35.0 112-113 29.901083099550455 35.0 32.0 35.0 2.0 35.0 114-115 29.805991586304234 35.0 32.0 35.0 2.0 35.0 116-117 29.721439582847868 35.0 32.0 35.0 2.0 35.0 118-119 29.61533753879911 35.0 32.0 35.0 2.0 35.0 120-121 29.47357762849213 35.0 32.0 35.0 2.0 35.0 122-123 29.3027574631538 35.0 32.0 35.0 2.0 35.0 124-125 28.443939792236613 34.5 29.5 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.02026929439727354 1101 2 0.16416655053020435 1101 3 0.12615202075225795 1101 4 0.13057862415710275 1101 5 0.009635853767790081 1101 6 0.09778802089743266 1101 7 0.12560099523612678 1101 8 0.1832038994388867 1101 9 0.11203224667171696 1101 10-11 0.13764607362957548 1101 12-13 0.1426166807596161 1101 14-15 0.21131783690771044 1101 16-17 0.2936446750912083 1101 18-19 0.34332357093627053 1101 20-21 0.2479913954909989 1101 22-23 0.2790678075610771 1101 24-25 0.2744099528366064 1101 26-27 0.34339840187833204 1101 28-29 0.2677194095674409 1101 30-31 0.3204220313162125 1101 32-33 0.3468838544052275 1101 34-35 0.3533294293295839 1101 36-37 0.35926641087765177 1101 38-39 0.3318952042480774 1101 40-41 0.3707489983336387 1101 42-43 0.4553699248067673 1101 44-45 0.39819654892829703 1101 46-47 0.2290558542637129 1101 48-49 0.27228411046761636 1101 50-51 0.16928481961590336 1101 52-53 0.08640679683285413 1101 54-55 0.11417132186443268 1101 56-57 0.17950449701438487 1101 58-59 0.0791713655751991 1101 60-61 0.11740881244737977 1101 62-63 -0.029215895187050478 1101 64-65 0.0693538110814913 1101 66-67 0.03647833807833223 1101 68-69 0.14802501726812523 1101 70-71 0.19662181414490476 1101 72-73 0.11410070703422548 1101 74-75 0.06910789387166005 1101 76-77 0.11239790094354873 1101 78-79 0.1663701444351524 1101 80-81 0.05402784174520292 1101 82-83 0.0343633131104788 1101 84-85 0.059026091301237926 1101 86-87 0.041770890038034025 1101 88-89 0.05460585539714913 1101 90-91 0.115987383623402 1101 92-93 0.07381927973627 1101 94-95 0.14732361126117866 1101 96-97 0.1475822238501081 1101 98-99 0.17292207999662068 1101 100-101 -0.08655897471995644 1101 102-103 0.016399289425812924 1101 104-105 0.06257282607369419 1101 106-107 0.01668174220041152 1101 108-109 0.05020575599140642 1101 110-111 0.06178580669221745 1101 112-113 0.013972772445821846 1101 114-115 0.13464228241844012 1101 116-117 0.06686620420034117 1101 118-119 0.048662288554048416 1101 120-121 0.007872428996797964 1101 122-123 -0.040458629561651804 1101 124-125 0.056987417303592025 1102 1 0.2275163451600406 1102 2 0.26252873307973346 1102 3 0.15436003470565396 1102 4 0.12192353364956432 1102 5 0.20303306634538387 1102 6 0.1330837782813532 1102 7 0.2349476332717657 1102 8 0.2076073599119752 1102 9 0.2362043663254596 1102 10-11 0.27906536127960635 1102 12-13 0.29891811289735415 1102 14-15 0.26899070587904816 1102 16-17 0.27249086275472933 1102 18-19 0.33438809691880067 1102 20-21 0.23552149356986973 1102 22-23 0.289264844548903 1102 24-25 0.352044108693363 1102 26-27 0.2877064538023646 1102 28-29 0.25922795751953487 1102 30-31 0.37295746677687447 1102 32-33 0.4479873475083167 1102 34-35 0.5123996893339964 1102 36-37 0.5999051512164684 1102 38-39 0.5540061631727085 1102 40-41 0.4697717592221835 1102 42-43 0.5531153287641857 1102 44-45 0.5255408123337943 1102 46-47 0.5426814182058024 1102 48-49 0.4799698099967884 1102 50-51 0.3635358830216546 1102 52-53 0.3982331518407136 1102 54-55 0.42248474178123274 1102 56-57 0.5384040194180386 1102 58-59 0.5842303177549937 1102 60-61 0.5325345095797758 1102 62-63 0.5376941612999673 1102 64-65 0.3795796028474072 1102 66-67 0.4459050205585058 1102 68-69 0.44225006134467293 1102 70-71 0.5181906005890653 1102 72-73 0.4289398007669831 1102 74-75 0.3305650284108097 1102 76-77 0.3847584044485117 1102 78-79 0.2927692125589054 1102 80-81 0.23992438837125363 1102 82-83 0.30790152505175783 1102 84-85 0.3145512095605554 1102 86-87 0.29454297439145805 1102 88-89 0.32909121651222506 1102 90-91 0.33380580452692143 1102 92-93 0.3665100300764088 1102 94-95 0.3405045931179842 1102 96-97 0.38754149822670314 1102 98-99 0.3533363311642326 1102 100-101 0.3510558571443738 1102 102-103 0.5059321268613424 1102 104-105 0.438283147247958 1102 106-107 0.362360091187675 1102 108-109 0.4612395146870405 1102 110-111 0.43056355603000895 1102 112-113 0.47049672032379775 1102 114-115 0.5386531017700449 1102 116-117 0.4901482608931751 1102 118-119 0.5399769127196592 1102 120-121 0.5469600395626664 1102 122-123 0.5776521912879389 1102 124-125 0.559907346155871 1103 1 0.2809199409806524 1103 2 0.27825861545751707 1103 3 0.21716617456007015 1103 4 0.1164915825947972 1103 5 0.2047036087878169 1103 6 0.25063757238724094 1103 7 0.19664441173476632 1103 8 0.19730747359488277 1103 9 0.29115240747026405 1103 10-11 0.2724628575186898 1103 12-13 0.32251880144627876 1103 14-15 0.29183872170668224 1103 16-17 0.35306154507699006 1103 18-19 0.2793672322444749 1103 20-21 0.31995475544816543 1103 22-23 0.38373406854332615 1103 24-25 0.263423249493016 1103 26-27 0.3851106126612791 1103 28-29 0.39534833041510353 1103 30-31 0.4425831147352355 1103 32-33 0.4017771524096787 1103 34-35 0.40755824424147136 1103 36-37 0.5904938097093009 1103 38-39 0.6174442548638908 1103 40-41 0.7061207686755608 1103 42-43 0.7074547802514886 1103 44-45 0.6636044493461313 1103 46-47 0.7292445483859353 1103 48-49 0.7651487066176799 1103 50-51 0.9453606940470394 1103 52-53 0.9632909561282119 1103 54-55 0.8787300192486569 1103 56-57 0.8796809824520366 1103 58-59 0.8818151180670029 1103 60-61 0.9509530953175158 1103 62-63 0.9862856369392787 1103 64-65 0.9048645187973179 1103 66-67 0.8960937263621958 1103 68-69 0.8188733173677605 1103 70-71 0.7110781366220493 1103 72-73 0.688681780648146 1103 74-75 0.7126050842845402 1103 76-77 0.6602009417962265 1103 78-79 0.6153437247145668 1103 80-81 0.562577100290163 1103 82-83 0.639486604927928 1103 84-85 0.7145214936505191 1103 86-87 0.5849187340288502 1103 88-89 0.5271741493161457 1103 90-91 0.46696973762311345 1103 92-93 0.5758674137469271 1103 94-95 0.6654363393871279 1103 96-97 0.6554964082432093 1103 98-99 0.604948956907009 1103 100-101 0.6165655583137557 1103 102-103 0.7660206294433998 1103 104-105 0.6908845112473294 1103 106-107 0.6910068149597208 1103 108-109 0.6165300707948838 1103 110-111 0.7067621873386543 1103 112-113 0.7127390594351155 1103 114-115 0.7448790736709157 1103 116-117 0.7413136817398644 1103 118-119 0.6598713234893268 1103 120-121 0.6564861392223982 1103 122-123 0.6021120997355673 1103 124-125 0.8113723698455111 1104 1 0.12028171749056327 1104 2 0.18802634349935943 1104 3 0.20268541868568235 1104 4 0.15373527004614118 1104 5 0.12916185064337782 1104 6 0.1462219346997955 1104 7 0.13972822634603688 1104 8 0.07508758201642962 1104 9 0.13033425828109557 1104 10-11 0.20087666798141868 1104 12-13 0.21720353526345093 1104 14-15 0.3246485924491793 1104 16-17 0.3389942454074699 1104 18-19 0.2555429254944599 1104 20-21 0.2643922079613006 1104 22-23 0.2647402540718957 1104 24-25 0.18358704713719476 1104 26-27 0.23034840824517033 1104 28-29 0.2042322783391981 1104 30-31 0.1899781541930281 1104 32-33 0.19158990848916346 1104 34-35 0.19076455748931664 1104 36-37 0.18592574791928485 1104 38-39 0.013483558840427179 1104 40-41 0.10728888733329711 1104 42-43 0.2601608907333315 1104 44-45 0.2969312568769098 1104 46-47 0.3126829937533131 1104 48-49 0.21343778102581723 1104 50-51 0.3473655138183531 1104 52-53 0.3410403000519864 1104 54-55 0.309727581395272 1104 56-57 0.3927666149654456 1104 58-59 0.36039323891554886 1104 60-61 0.4362327492635245 1104 62-63 0.38271089552420534 1104 64-65 0.3333869372597462 1104 66-67 0.3706263157540306 1104 68-69 0.27200546953083915 1104 70-71 0.08512129082210151 1104 72-73 0.147146445757933 1104 74-75 0.21112372710405936 1104 76-77 0.20085715785622682 1104 78-79 0.16651533933795903 1104 80-81 0.145228254191899 1104 82-83 0.11027105300817652 1104 84-85 0.12104962685147314 1104 86-87 0.10675919680147317 1104 88-89 0.2020400207671571 1104 90-91 0.12372277794791131 1104 92-93 0.15239503420625056 1104 94-95 0.2172032004294806 1104 96-97 0.22620161443526499 1104 98-99 0.14353929032890633 1104 100-101 0.12433266174306468 1104 102-103 0.2013998018784129 1104 104-105 0.2221533603288215 1104 106-107 0.11959495757647431 1104 108-109 0.10815979675778564 1104 110-111 0.06549487648204533 1104 112-113 0.207957510675719 1104 114-115 0.2994119427400115 1104 116-117 0.24146097842615077 1104 118-119 0.28055299017930224 1104 120-121 0.22926282159760447 1104 122-123 0.32819480029911574 1104 124-125 0.38287978362334485 1105 1 0.05240323914221534 1105 2 0.15477813103765925 1105 3 0.11142810602898123 1105 4 0.023757246707546642 1105 5 0.0606859785413576 1105 6 0.012948959802713489 1105 7 0.07629770045381434 1105 8 0.05397141524142768 1105 9 -0.015203860392873025 1105 10-11 -0.0062758067144699226 1105 12-13 0.05480838870078486 1105 14-15 0.021665492811337117 1105 16-17 0.07056789817622189 1105 18-19 0.1151592625112059 1105 20-21 0.14094295547732116 1105 22-23 0.13584326580309636 1105 24-25 0.1594529585495934 1105 26-27 0.10253632417669678 1105 28-29 0.05664274665875979 1105 30-31 0.054382927152452964 1105 32-33 0.09016761610670443 1105 34-35 0.14103384897413918 1105 36-37 0.30797592280397623 1105 38-39 0.329552057927323 1105 40-41 0.16867883378452575 1105 42-43 0.11510099659274431 1105 44-45 0.22156214182444245 1105 46-47 0.26697308568385125 1105 48-49 0.22279333864490525 1105 50-51 0.12327630714488436 1105 52-53 0.04888225380814504 1105 54-55 0.27716265738499857 1105 56-57 0.1427194115729904 1105 58-59 -0.00455011701350827 1105 60-61 0.04508067695721252 1105 62-63 0.09387101562201394 1105 64-65 0.02684655901354205 1105 66-67 0.07659715798637023 1105 68-69 -0.005336902091414686 1105 70-71 0.058820489138255994 1105 72-73 -0.058592248642661104 1105 74-75 -0.05899497782056784 1105 76-77 -0.050258699388336936 1105 78-79 -0.007046117931363227 1105 80-81 -0.0035266596038709963 1105 82-83 0.08981186459699586 1105 84-85 0.08730693950817425 1105 86-87 0.08867720218730213 1105 88-89 0.06714433252621888 1105 90-91 0.11612767471950747 1105 92-93 0.04444397096918351 1105 94-95 0.08542293609048457 1105 96-97 0.14354228364008037 1105 98-99 0.157246845967002 1105 100-101 0.164655192463961 1105 102-103 0.13579714610721538 1105 104-105 0.14320220423262242 1105 106-107 0.21220414444000113 1105 108-109 0.16309448583445985 1105 110-111 0.2579887632558133 1105 112-113 0.24207627750643468 1105 114-115 0.2421147625623412 1105 116-117 0.21727504348945104 1105 118-119 0.19105458426107802 1105 120-121 0.2989102664237997 1105 122-123 0.34006529276204844 1105 124-125 0.43696094250531914 1106 1 0.20373269361595447 1106 2 0.2305536461473423 1106 3 0.17385545445452522 1106 4 0.0745390793454419 1106 5 0.038214376601111155 1106 6 0.050003882235799324 1106 7 0.07566194139509719 1106 8 0.10570734872555931 1106 9 0.13867941633951375 1106 10-11 0.18291336165228245 1106 12-13 0.18575626447044158 1106 14-15 0.2365987427327525 1106 16-17 0.22619573721176778 1106 18-19 0.22337559316755318 1106 20-21 0.16079401589754383 1106 22-23 0.17808549524340123 1106 24-25 0.34544222183335194 1106 26-27 0.22994160747931858 1106 28-29 0.16430868193028658 1106 30-31 0.2063307289608005 1106 32-33 0.2547472385342431 1106 34-35 0.18726428774957782 1106 36-37 0.14487105799908306 1106 38-39 0.15353817169213357 1106 40-41 0.08956781786435641 1106 42-43 0.042656821970098235 1106 44-45 -0.009286810698561965 1106 46-47 0.05632234946068593 1106 48-49 0.0906617738085771 1106 50-51 0.18401672145146364 1106 52-53 0.22789604142825226 1106 54-55 0.22027886292346466 1106 56-57 0.2328602154091186 1106 58-59 0.1176895854155049 1106 60-61 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16-17 -0.7396016232254752 2216 18-19 -0.6563057680338673 2216 20-21 -0.6516518148428361 2216 22-23 -0.6019224612603935 2216 24-25 -0.6636907921743784 2216 26-27 -0.7060312804143791 2216 28-29 -0.7617458683121114 2216 30-31 -0.7402510355488161 2216 32-33 -0.8283283976725713 2216 34-35 -0.899469225678736 2216 36-37 -0.9119318879726706 2216 38-39 -0.9149713582865004 2216 40-41 -0.9778238430017154 2216 42-43 -0.9955610736231648 2216 44-45 -0.940293276464331 2216 46-47 -0.8329752923800982 2216 48-49 -0.884354410890495 2216 50-51 -0.7632629582623807 2216 52-53 -0.7813815468757852 2216 54-55 -0.8029486697268453 2216 56-57 -0.7941392803019696 2216 58-59 -0.8847145769473315 2216 60-61 -0.7987382886902736 2216 62-63 -0.8771705891402988 2216 64-65 -0.94666660332107 2216 66-67 -1.0201438610558284 2216 68-69 -1.0170195757551568 2216 70-71 -0.9227839709970169 2216 72-73 -0.8254489511844731 2216 74-75 -0.7644541864185186 2216 76-77 -0.6235678921219758 2216 78-79 -0.7600225632196675 2216 80-81 -0.6968151783816694 2216 82-83 -0.6428233974661701 2216 84-85 -0.666818912253774 2216 86-87 -0.7229154478562592 2216 88-89 -0.7572087564800718 2216 90-91 -0.7707509104869033 2216 92-93 -0.683474808839339 2216 94-95 -0.7072425940542075 2216 96-97 -0.8645446074099326 2216 98-99 -0.9014971545567683 2216 100-101 -0.9058719556381121 2216 102-103 -1.0037426128561115 2216 104-105 -0.9239598231748509 2216 106-107 -1.0309685397495834 2216 108-109 -1.1649469275489714 2216 110-111 -1.2368278907361976 2216 112-113 -1.2765115225932817 2216 114-115 -1.3046552151269104 2216 116-117 -1.4788476454846062 2216 118-119 -1.6305595203627377 2216 120-121 -1.7278485307095437 2216 122-123 -1.8750627044579176 2216 124-125 -2.400074900745299 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5.0 3 14.0 4 23.0 5 115.0 6 398.0 7 862.0 8 1511.0 9 2893.0 10 4776.0 11 6944.0 12 8930.0 13 13499.0 14 20747.0 15 19659.0 16 8006.0 17 5765.0 18 5248.0 19 4856.0 20 4689.0 21 4513.0 22 4638.0 23 4940.0 24 6011.0 25 7488.0 26 9361.0 27 11589.0 28 14644.0 29 18282.0 30 23100.0 31 29163.0 32 37044.0 33 48349.0 34 65492.0 35 99125.0 36 179966.0 37 358996.0 38 173116.0 39 1134.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.77782835536365 18.81538802745477 13.831714527553201 21.575069089628375 2 16.669734995870193 21.06267020934298 38.90566795038926 23.361926844397562 3 19.176277126207925 26.433732402016435 30.72906257696591 23.66092789480973 4 12.40427202790302 17.080731177195947 36.551479362562624 33.96351743233841 5 12.042216087523665 39.28240612128294 33.634549059575036 15.040828731618364 6 30.42422573847885 37.345000501703716 16.664524405605484 15.566249354211948 7 26.725881526605637 35.3650537237611 20.342634616229823 17.566430133403433 8 27.229741328196326 34.1561550753758 19.06457548816601 19.549528108261857 9 27.78271649984659 14.599590344061234 19.8619277048487 37.75576545124348 10-11 25.039193190398258 27.150255973722214 26.296277714592303 21.514273121287218 12-13 26.594543490565453 25.334184768298424 27.605235495715526 20.4660362454206 14-15 23.22150177752384 25.529131571593123 27.494110164185653 23.755256486697387 16-17 20.668238677520645 29.119448637993706 28.12752278095824 22.084789903527405 18-19 20.486884801362642 25.505870762780386 33.25988833153245 20.74735610432452 20-21 21.63342292130881 24.381391021244873 33.928190856387516 20.056995201058804 22-23 21.306320388824528 24.751200564561806 33.736506865048334 20.205972181565333 24-25 20.693536978051913 24.90826285294442 33.981139257196546 20.41706091180712 26-27 20.55509162934295 25.076686035470868 33.88407409956621 20.48414823561997 28-29 20.55011605526536 24.973981893885934 34.01501462404148 20.460887426807233 30-31 20.586852377204906 24.73494702257501 34.090021403261154 20.58817919695893 32-33 20.469180050269884 24.847560849197812 33.917203130299505 20.7660559702328 34-35 20.89861355628328 24.941433346795026 33.73480687723849 20.425146219683203 36-37 21.09340728142096 24.681542527475532 33.306078244219414 20.91897194688409 38-39 20.871289361973844 24.7946539115061 33.31656841289967 21.017488313620387 40-41 21.0766561820264 24.789014927551495 33.21684961576129 20.91747927466081 42-43 21.049166135247713 24.830809749803258 32.699721616630356 21.420302498318673 44-45 21.15862876495471 25.13921241637926 32.4380478832664 21.264110935399632 46-47 21.628779052169726 25.50176591416637 31.283880549734594 21.58557448392931 48-49 21.735505116134046 25.912831259209995 30.299007124192816 22.052656500463144 50-51 21.8063655836224 26.282972507465434 29.7944424496078 22.116219459304364 52-53 22.000827603821573 26.38426690306172 29.584182981712274 22.03072251140443 54-55 21.730819783877646 26.314152771685002 29.60408527802264 22.350942166414708 56-57 21.842355569450305 26.538136531411215 29.24451712468208 22.3749907744564 58-59 21.85537498828667 26.08465441735613 29.531110191551306 22.528860402805893 60-61 21.77518531940283 26.08884219220477 29.967426575038708 22.168545913353693 62-63 21.82875566697156 26.0898787701376 30.05014549407865 22.03122006881219 64-65 21.566128281909393 25.960513844120243 30.052177186827002 22.421180687143366 66-67 21.61497183410441 26.21986564291466 29.74808668445158 22.417075838529353 68-69 21.887135736148625 25.983899042284914 29.780759620894425 22.34820560067203 70-71 21.755697654265603 26.101861611041134 29.623407090690616 22.51903364400265 72-73 21.838126331484357 26.230272885360286 29.325743371498753 22.60585741165661 74-75 21.62853027346584 26.587187399192796 29.023394320050482 22.760888007290873 76-77 21.687490826285295 26.444595738752508 29.09064749633259 22.77726593862961 78-79 21.662447103428086 26.492817344187824 29.017506557392004 22.827228994992087 80-81 21.48125328076916 26.743835056402276 28.835981029794567 22.938930633033998 82-83 21.57068922481385 26.626660286875016 28.720216006255956 23.082434482055177 84-85 21.673725071337294 26.253284915469145 28.54428799949581 23.528702013697757 86-87 21.571394097808177 26.45471273937694 28.456966674434092 23.51692648838079 88-89 21.542577231275466 26.32074540733781 28.527205195162747 23.60947216622398 90-91 21.528230992685078 26.45205909986889 28.430886373644054 23.58882353380198 92-93 21.460954150046856 26.209936229672692 28.481785237459466 23.847324382820986 94-95 21.373176336344404 26.089429903232737 28.549079802932788 23.98831395749007 96-97 21.501198565453368 26.011918065476795 28.420286171237496 24.06659719783234 98-99 21.406998295829137 25.987112985284423 28.25386755565507 24.352021163231374 100-101 21.30079864726295 26.103048343846652 28.382350563066844 24.213802445823553 102-103 21.39810057089024 25.79762090367181 28.349253132266742 24.455025393171212 104-105 21.23540851997296 25.939971221350937 28.24284369544393 24.581776563232165 106-107 21.263559854122967 26.04673904118281 28.034135292073394 24.655565812620836 108-109 21.358664005693555 26.030007282837886 27.79767148262985 24.81365722883871 110-111 21.290624108392883 26.234938060261033 27.77426664277989 24.7001711885662 112-113 21.15601393839204 26.218054049839456 27.76654769320607 24.85938431856243 114-115 20.984464339483505 26.336963491322212 27.58780439451238 25.09076777468191 116-117 20.89403241533165 26.36761900890988 27.665592625572188 25.07275595018628 118-119 20.81482058984773 26.450380318171753 27.48218671129907 25.252612380681445 120-121 20.919728335791294 26.52612047385298 27.427783206122065 25.126367984233656 122-123 20.89125724416061 26.617373544688306 27.211171817493806 25.28019739365728 124-125 20.85353084003243 26.57167324308129 27.038622871823154 25.536173045063126 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1469.0 1 1011.5 2 746.0 3 950.0 4 643.0 5 442.5 6 1104.5 7 2604.5 8 4220.5 9 4872.5 10 3726.0 11 2281.5 12 2005.5 13 2156.5 14 2375.5 15 2562.0 16 2767.0 17 3004.5 18 3250.5 19 3521.5 20 3770.0 21 4159.0 22 4738.5 23 5489.0 24 6441.5 25 7608.5 26 9045.0 27 10565.5 28 12097.5 29 14004.0 30 16177.0 31 18471.5 32 21544.5 33 23555.0 34 24729.0 35 26132.5 36 27988.0 37 30654.0 38 31665.0 39 31679.0 40 32497.5 41 33873.0 42 34727.0 43 35612.5 44 37245.5 45 39147.5 46 40111.5 47 40550.5 48 40977.5 49 41170.5 50 41108.5 51 39474.5 52 37914.5 53 38190.0 54 38222.0 55 35987.5 56 33361.0 57 30933.5 58 28792.0 59 26574.0 60 22833.5 61 19199.5 62 16197.5 63 12107.5 64 8558.5 65 6551.5 66 4793.0 67 3742.5 68 2772.5 69 1713.0 70 1237.5 71 927.0 72 758.0 73 747.0 74 580.0 75 468.5 76 352.5 77 166.5 78 78.0 79 48.0 80 36.0 81 19.5 82 10.5 83 7.0 84 4.5 85 4.5 86 3.5 87 2.5 88 1.5 89 0.5 90 1.0 91 0.5 92 1.5 93 1.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1675109939455556 2 0.0019073033964097917 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 8.292623462651267E-5 10-11 4.560942904458197E-4 12-13 0.006924340591313809 14-15 0.0 16-17 3.73168055819307E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 8.292623462651267E-5 94-95 6.634098770121014E-4 96-97 0.0061780044796751945 98-99 0.00215608210028933 100-101 0.0032755862677472506 102-103 0.004560942904458197 104-105 0.011277967909205724 106-107 0.018077919148579764 108-109 0.02628761637660452 110-111 0.016834025629182073 112-113 0.024877870387953804 114-115 0.028360772242267334 116-117 0.0264949319631708 118-119 0.01575598457903741 120-121 0.023633976868556113 122-123 0.014802332880832512 124-125 0.016046226400230203 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1205891.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 64.20464877877774 #Duplication Level Percentage of deduplicated Percentage of total 1 82.70640977781805 53.10135991538477 2 10.619636603210736 13.636600765347954 3 3.0834443606253994 5.939143866085699 4 1.299093873126968 3.336314634191161 5 0.6630866901608715 2.128662402583049 6 0.3966301917582034 1.527930129413683 7 0.2549930404264137 1.1460217021127397 8 0.16555182030722004 0.850335718200991 9 0.12224587494543127 0.706387811897322 >10 0.6214058030232583 7.037801415254198 >50 0.0407958660622475 1.8031139174351445 >100 0.022500413729289088 2.519598758483883 >500 0.002313126643268316 0.9986856718334017 >1k 0.001261705441782718 1.3597084495111786 >5k 2.1028424029711964E-4 0.8754194803525452 >10k+ 4.205684805942393E-4 3.0329153619123344 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11370 0.9428712877034491 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11094 0.9199836469465317 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6484 0.5376937053183082 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3206 0.2658615082125997 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2210 0.18326697852459303 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1306 0.10830166242222555 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 1.6585246925302535E-4 0.0 16-17 0.0 0.0 0.0 3.317049385060507E-4 0.0 18-19 0.0 0.0 0.0 3.731680558193071E-4 0.0 20-21 0.0 0.0 0.0 4.975574077590761E-4 0.0 22-23 0.0 0.0 0.0 7.463361116386142E-4 0.0 24-25 0.0 0.0 0.0 0.0011609672847711775 0.0 26-27 0.0 0.0 0.0 0.0020316927483495606 0.0 28-29 0.0 0.0 0.0 0.004768258491024479 0.0 30-31 1.6585246925302535E-4 0.0 0.0 0.014677943528892744 0.0 32-33 2.073155865662817E-4 0.0 0.0 0.03681924817417163 0.0 34-35 2.4877870387953805E-4 0.0 0.0 0.06775073368986086 0.0 36-37 2.4877870387953805E-4 0.0 0.0 0.12123815502396153 0.0 38-39 2.4877870387953805E-4 0.0 0.0 0.20743997591822147 0.0 40-41 2.4877870387953805E-4 0.0 0.0 0.29899053894589145 0.0 42-43 2.4877870387953805E-4 0.0 0.0 0.3783094823661508 0.0 44-45 2.4877870387953805E-4 0.0 0.0 0.4801014353701951 0.0 46-47 2.902418211927944E-4 0.0 0.0 0.6106687917896394 0.0 48-49 4.146311731325634E-4 0.0 0.0 0.753758009637687 0.0 50-51 4.146311731325634E-4 0.0 0.0 0.9237982537393512 0.0 52-53 4.146311731325634E-4 0.0 0.0 1.0747654638769175 0.0 54-55 4.146311731325634E-4 0.0 0.0 1.2336521294213159 0.0 56-57 4.146311731325634E-4 0.0 0.0 1.431223883418982 0.0 58-59 4.146311731325634E-4 0.0 0.0 1.6602661434574104 0.0 60-61 4.146311731325634E-4 0.0 0.0 1.9207789095366 0.0 62-63 4.146311731325634E-4 0.0 0.0 2.1792185197501266 0.0 64-65 4.146311731325634E-4 0.0 0.0 2.4287850228586167 0.0 66-67 4.975574077590761E-4 0.0 0.0 2.7303048119606164 0.0 68-69 4.975574077590761E-4 0.0 0.0 3.0821193623635965 0.0 70-71 4.975574077590761E-4 0.0 0.0 3.474899472672074 0.0 72-73 4.975574077590761E-4 0.0 0.0 3.8706234643097925 0.0 74-75 4.975574077590761E-4 0.0 0.0 4.246776864575654 0.0 76-77 4.975574077590761E-4 0.0 0.0 4.656059295574808 0.0 78-79 4.975574077590761E-4 0.0 0.0 5.135455857950677 0.0 80-81 6.634098770121014E-4 0.0 0.0 5.672527616509287 0.0 82-83 6.634098770121014E-4 0.0 0.0 6.232528478942127 0.0 84-85 6.634098770121014E-4 0.0 0.0 6.770761204785507 0.0 86-87 6.634098770121014E-4 0.0 0.0 7.313264631712153 0.0 88-89 6.634098770121014E-4 0.0 0.0 7.924099275971045 0.0 90-91 7.463361116386142E-4 0.0 0.0 8.615330904700343 0.0 92-93 7.463361116386142E-4 0.0 0.0 9.334508674498773 0.0 94-95 7.463361116386142E-4 0.0 0.0 10.049457206331251 0.0 96-97 7.463361116386142E-4 0.0 0.0 10.738118121787126 0.0 98-99 9.121885808916395E-4 0.0 0.0 11.479893290521282 0.0 100-101 9.121885808916395E-4 0.0 0.0 12.32341894914217 0.0 102-103 9.536516982048958E-4 0.0 0.0 13.209112598070638 0.0 104-105 0.0010780410501446647 0.0 0.0 14.082367311805129 0.0 106-107 0.0010780410501446647 0.0 0.0 14.905244338004014 0.0 108-109 0.0011195041674579211 0.0 0.0 15.754367517462192 0.0 110-111 0.0011609672847711775 0.0 0.0 16.688614476764485 0.0 112-113 0.0011609672847711775 0.0 0.0 17.67270010307731 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5195 0.0 81.99875 1 GTATCAA 9530 0.0 52.826336 1 TGGTATC 1765 0.0 48.878212 2 GTGGTAT 1895 0.0 48.73143 1 ATCAACG 12565 0.0 40.00997 3 TCAACGC 12760 0.0 39.351906 4 TATCAAC 12850 0.0 39.170513 2 CAACGCA 13015 0.0 38.53518 5 AACGCAG 13295 0.0 38.0816 6 GTACATG 9455 0.0 35.349888 1 TACATGG 9550 0.0 34.451942 2 ACATGGG 9480 0.0 33.44975 3 ACGCAGA 15395 0.0 32.85377 7 CGCAGAG 15445 0.0 32.67036 8 GCAGAGT 16115 0.0 31.090506 9 CATGGGA 7425 0.0 30.287909 4 GAGTACT 11230 0.0 29.804977 12-13 CAGAGTA 15845 0.0 28.503328 10-11 GTACTTT 11780 0.0 28.18142 14-15 ATGGGAT 2650 0.0 28.063261 5 >>END_MODULE