FastQCFastQC Report
Thu 26 May 2016
SRR1364112_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364112_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2154111
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN89330.4146954358433711No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA26250.065.96931
GTATCAA47200.048.161451
ATCAACG55450.039.1760063
GTACATG116800.038.772161
CAACGCA56450.038.4874045
TCAACGC57800.037.4846154
AACGCAG58750.036.679286
TACATGG121800.036.450222
TATCAAC61800.036.304582
ACATGGG123000.034.6930853
ACGCAGA70550.030.1239247
CATGGGA76550.029.702824
TGGTATC13900.029.5422042
CGCAGAG71700.029.0595728
ATGGGAG34100.028.1035485
CATGGGG54000.027.8872874
GTGGTAT17500.025.5035951
GCAGAGT79900.025.1086549
GAGTACA86700.024.4348161
AGTACAT85700.023.332812
Produced by FastQC (version 0.11.5)