##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364112_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2154111 Sequences flagged as poor quality 0 Sequence length 125 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.146364323844036 31.0 31.0 34.0 28.0 34.0 2 31.14395729839363 33.0 31.0 34.0 28.0 34.0 3 31.212521546011324 33.0 31.0 34.0 28.0 34.0 4 34.66995479805822 37.0 35.0 37.0 32.0 37.0 5 34.706587543538845 37.0 35.0 37.0 32.0 37.0 6 34.75206059483472 37.0 35.0 37.0 32.0 37.0 7 34.63480526305283 37.0 35.0 37.0 31.0 37.0 8 34.60274656227093 37.0 35.0 37.0 31.0 37.0 9 36.09449605893104 39.0 37.0 39.0 31.0 39.0 10-11 36.013406226512934 39.0 37.0 39.0 30.5 39.0 12-13 35.917932037856914 39.0 37.0 39.0 30.0 39.0 14-15 37.18861586055686 40.0 37.5 41.0 31.0 41.0 16-17 37.21653573098136 40.0 38.0 41.0 31.0 41.0 18-19 37.22813471543481 40.0 37.5 41.0 31.0 41.0 20-21 37.258158237899536 40.0 38.0 41.0 31.0 41.0 22-23 37.23195369226563 40.0 37.0 41.0 31.0 41.0 24-25 37.257324251164405 40.0 37.0 41.0 31.0 41.0 26-27 37.23992078402645 40.0 37.0 41.0 31.0 41.0 28-29 37.205584345467805 40.0 37.0 41.0 31.0 41.0 30-31 37.16458019108579 40.0 37.0 41.0 31.0 41.0 32-33 37.07688902753851 40.0 37.0 41.0 30.0 41.0 34-35 37.05639820789179 40.0 37.0 41.0 30.0 41.0 36-37 36.99988347861368 40.0 37.0 41.0 30.0 41.0 38-39 36.922335014305204 40.0 37.0 41.0 30.0 41.0 40-41 36.84293288507416 40.0 37.0 41.0 30.0 41.0 42-43 36.72453856834676 40.0 36.0 41.0 30.0 41.0 44-45 36.625384671449154 40.0 36.0 41.0 30.0 41.0 46-47 36.509074044930834 40.0 36.0 41.0 30.0 41.0 48-49 36.39982804971517 40.0 36.0 41.0 30.0 41.0 50-51 36.270843285234605 40.0 35.0 41.0 29.5 41.0 52-53 36.12080691292138 39.5 35.0 41.0 29.0 41.0 54-55 35.939987540103544 39.0 35.0 41.0 29.0 41.0 56-57 35.755297661076895 39.0 35.0 41.0 28.0 41.0 58-59 35.54329628324631 39.0 35.0 41.0 28.0 41.0 60-61 35.30074309076923 38.0 35.0 40.5 28.0 41.0 62-63 35.03845228959882 38.0 34.0 40.0 28.0 41.0 64-65 34.75318356389248 37.0 34.0 40.0 27.5 41.0 66-67 34.44665014941198 37.0 34.0 40.0 27.0 41.0 68-69 34.12254800240099 36.0 34.0 39.0 26.0 41.0 70-71 33.7746499600067 36.0 33.5 39.0 26.0 41.0 72-73 33.42363856830033 35.0 33.0 38.5 26.0 40.0 74-75 33.14888044302266 35.0 33.0 37.5 26.0 40.0 76-77 32.91835239688206 35.0 33.0 37.0 26.0 39.0 78-79 32.617267169611964 35.0 33.0 37.0 26.0 39.0 80-81 32.4060238771354 35.0 33.0 36.0 26.0 39.0 82-83 32.30752732797892 35.0 33.0 36.0 26.0 37.0 84-85 32.0983700468546 35.0 33.0 35.5 26.0 37.0 86-87 31.898856883419658 35.0 33.0 35.0 25.5 36.5 88-89 31.720488405657832 35.0 33.0 35.0 25.0 36.0 90-91 31.575840567175973 35.0 33.0 35.0 25.0 36.0 92-93 31.454137460882933 35.0 33.0 35.0 25.0 36.0 94-95 31.34775343517581 35.0 33.0 35.0 24.0 36.0 96-97 31.258164737100365 35.0 33.0 35.0 24.0 35.0 98-99 31.17619263816953 35.0 33.0 35.0 24.0 35.0 100-101 31.095718837144418 35.0 33.0 35.0 23.0 35.0 102-103 30.004141151500548 34.0 30.5 35.0 19.5 35.0 104-105 30.805721478605328 35.0 32.0 35.0 20.0 35.0 106-107 30.887776906575382 35.0 33.0 35.0 20.0 35.0 108-109 30.847081000004177 35.0 33.0 35.0 19.5 35.0 110-111 30.7671301525316 35.0 33.0 35.0 19.0 35.0 112-113 30.676983451642002 35.0 33.0 35.0 17.5 35.0 114-115 30.556983135966533 35.0 32.5 35.0 14.5 35.0 116-117 30.446654559583976 35.0 32.0 35.0 5.0 35.0 118-119 30.296777649805417 35.0 32.0 35.0 2.0 35.0 120-121 30.135845367300014 35.0 32.0 35.0 2.0 35.0 122-123 29.953178132417502 35.0 32.0 35.0 2.0 35.0 124-125 29.109958818278166 34.5 30.5 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3970756060763989 1101 2 0.3990845202976985 1101 3 0.3896683688218836 1101 4 0.46974976806750135 1101 5 0.4012995990738162 1101 6 0.43331061605977794 1101 7 0.45994905549713394 1101 8 0.4538855220643683 1101 9 0.4676382126120444 1101 10-11 0.466379283112218 1101 12-13 0.5834456278611952 1101 14-15 0.6108179618601781 1101 16-17 0.5612294204020856 1101 18-19 0.5626941643515693 1101 20-21 0.5437135290863324 1101 22-23 0.6252186818694554 1101 24-25 0.6043403735445452 1101 26-27 0.5220232572357091 1101 28-29 0.643009749212915 1101 30-31 0.6412653924657263 1101 32-33 0.6802383233233016 1101 34-35 0.6249498422469841 1101 36-37 0.6108883354622492 1101 38-39 0.6363207855830595 1101 40-41 0.6414963176596089 1101 42-43 0.5744970382756378 1101 44-45 0.65740488108991 1101 46-47 0.576896484285605 1101 48-49 0.5036166319022684 1101 50-51 0.5923802525616821 1101 52-53 0.568641282780959 1101 54-55 0.5618972588516016 1101 56-57 0.5474421404523113 1101 58-59 0.5363474125382126 1101 60-61 0.5102992172448353 1101 62-63 0.5466929953208535 1101 64-65 0.5557310904715465 1101 66-67 0.5543333768091614 1101 68-69 0.5811453944948894 1101 70-71 0.49706296945025485 1101 72-73 0.6133863613080095 1101 74-75 0.5737618630242025 1101 76-77 0.5319676857844513 1101 78-79 0.5239230367442289 1101 80-81 0.5550815954881969 1101 82-83 0.5157560259680238 1101 84-85 0.5651454907988764 1101 86-87 0.49658633891381854 1101 88-89 0.4916166124690342 1101 90-91 0.4949166933931437 1101 92-93 0.5392058592520748 1101 94-95 0.5542945500527772 1101 96-97 0.4498191050711178 1101 98-99 0.3591311886376225 1101 100-101 0.3970299177605874 1101 102-103 0.4773234812742082 1101 104-105 0.3689714612297479 1101 106-107 0.4238613958101034 1101 108-109 0.45681693336851836 1101 110-111 0.44202811846632883 1101 112-113 0.5182470629584977 1101 114-115 0.5006934379727674 1101 116-117 0.550106521599659 1101 118-119 0.6413624518219052 1101 120-121 0.6069800447711771 1101 122-123 0.6631118179513571 1101 124-125 0.671107126794567 1102 1 0.23188399935869342 1102 2 0.24651468323479975 1102 3 0.2564785974728885 1102 4 0.30957734929540237 1102 5 0.25514914055251126 1102 6 0.24739089418872595 1102 7 0.21905891771613284 1102 8 0.2668620120179739 1102 9 0.3182072731727956 1102 10-11 0.31788788720522376 1102 12-13 0.34746137128178134 1102 14-15 0.4315047250655297 1102 16-17 0.48045294232061764 1102 18-19 0.3219658646987469 1102 20-21 0.3226869659746825 1102 22-23 0.3643322100128401 1102 24-25 0.3829319767326993 1102 26-27 0.3876328354859453 1102 28-29 0.43861320689354955 1102 30-31 0.5013173250576415 1102 32-33 0.5396757924396951 1102 34-35 0.4317560279529289 1102 36-37 0.37764349503023453 1102 38-39 0.3659907481055953 1102 40-41 0.43010022616070387 1102 42-43 0.42208282251071694 1102 44-45 0.49888172720771706 1102 46-47 0.47207069992217754 1102 48-49 0.48574785755636185 1102 50-51 0.49836994786480204 1102 52-53 0.48051370379914005 1102 54-55 0.40459761726189214 1102 56-57 0.4272276959080443 1102 58-59 0.4077105668696177 1102 60-61 0.40954565720709724 1102 62-63 0.41520322621794037 1102 64-65 0.35983275449639507 1102 66-67 0.4074268715180409 1102 68-69 0.30927578909916065 1102 70-71 0.4717771155866757 1102 72-73 0.42228240467661493 1102 74-75 0.2864805350130126 1102 76-77 0.3416482530160607 1102 78-79 0.3208913894687271 1102 80-81 0.34261360213660197 1102 82-83 0.25136907762145455 1102 84-85 0.26625513482719043 1102 86-87 0.2606518169760328 1102 88-89 0.2828091288493759 1102 90-91 0.3332195468781052 1102 92-93 0.2570786845647639 1102 94-95 0.29210454928269414 1102 96-97 0.3432363642752314 1102 98-99 0.3970197605795853 1102 100-101 0.3238726664498408 1102 102-103 0.3373294895397869 1102 104-105 0.2976327330247521 1102 106-107 0.32562665471902363 1102 108-109 0.2841625233497176 1102 110-111 0.2746317429986611 1102 112-113 0.25051325399085655 1102 114-115 0.2846978948383949 1102 116-117 0.29645142652627854 1102 118-119 0.2632387954594009 1102 120-121 0.32377615520132963 1102 122-123 0.3056342546921016 1102 124-125 0.31836779086433964 1103 1 0.26464422506481355 1103 2 0.32678235917571996 1103 3 0.3239154011397254 1103 4 0.2819537917614454 1103 5 0.33694019474840786 1103 6 0.323813736184583 1103 7 0.28266489052647614 1103 8 0.29436013343461553 1103 9 0.4386113510299694 1103 10-11 0.4335446715044071 1103 12-13 0.5166184839632493 1103 14-15 0.639993850976964 1103 16-17 0.5999821722888115 1103 18-19 0.5648207887566485 1103 20-21 0.5916363990468199 1103 22-23 0.6342261119636134 1103 24-25 0.6500393626716274 1103 26-27 0.6971610990437966 1103 28-29 0.6457875709856822 1103 30-31 0.6324465853987249 1103 32-33 0.4425443054103013 1103 34-35 0.7029514775408217 1103 36-37 0.6447210504459804 1103 38-39 0.7149041281553323 1103 40-41 0.7643139625989122 1103 42-43 0.785257707281076 1103 44-45 0.7072471920412298 1103 46-47 0.7517288963638222 1103 48-49 0.7160204148556133 1103 50-51 0.6901499869551202 1103 52-53 0.6582463276426509 1103 54-55 0.736354058189562 1103 56-57 0.8390047864606913 1103 58-59 0.805786572084294 1103 60-61 0.7573463833954364 1103 62-63 0.7706976141350452 1103 64-65 0.7144262606133722 1103 66-67 0.7919686944046092 1103 68-69 0.8122203329239 1103 70-71 0.7454317836444417 1103 72-73 0.6561683505063556 1103 74-75 0.7614170773546078 1103 76-77 0.7784433404959259 1103 78-79 0.8013467758257704 1103 80-81 0.7069099173520428 1103 82-83 0.6507774471294354 1103 84-85 0.6861537702163574 1103 86-87 0.710449265258287 1103 88-89 0.6419196664693665 1103 90-91 0.6445574137935424 1103 92-93 0.6864245233793547 1103 94-95 0.701287123918167 1103 96-97 0.7451240200595954 1103 98-99 0.7657643245214381 1103 100-101 0.7702388156596527 1103 102-103 0.7845810875683057 1103 104-105 0.7482893496813254 1103 106-107 0.7828790712379323 1103 108-109 0.7804710330858065 1103 110-111 0.8635041196383533 1103 112-113 0.8299392654181652 1103 114-115 0.9439013261867721 1103 116-117 0.9522014201164133 1103 118-119 0.9924463784276973 1103 120-121 1.0314723315692476 1103 122-123 1.0782801086931393 1103 124-125 1.1270336811156234 1104 1 0.24930674289159782 1104 2 0.1748139921642924 1104 3 0.24602041602577884 1104 4 0.3347285741172499 1104 5 0.2672669683574682 1104 6 0.1570854398906576 1104 7 0.21425430528820755 1104 8 0.1655626407277282 1104 9 0.1780798304738198 1104 10-11 0.21127580509312338 1104 12-13 0.23251208191088324 1104 14-15 0.201342530420618 1104 16-17 0.23786040746946213 1104 18-19 0.33532736430474586 1104 20-21 0.20821388597717316 1104 22-23 0.19556204906540842 1104 24-25 0.2684468497718768 1104 26-27 0.31271135334096556 1104 28-29 0.3930182853263702 1104 30-31 0.4053554671607884 1104 32-33 -0.2736054885635184 1104 34-35 0.23630892971206663 1104 36-37 0.37008650580730773 1104 38-39 0.3424580682373488 1104 40-41 0.43798673526254106 1104 42-43 0.45961761697535053 1104 44-45 0.43252280140000465 1104 46-47 0.42424496420704116 1104 48-49 0.4881111883007776 1104 50-51 0.3768522289077083 1104 52-53 0.3171483775106765 1104 54-55 0.294757657626306 1104 56-57 0.28739930135724023 1104 58-59 0.37203525662487635 1104 60-61 0.3632654760207714 1104 62-63 0.4177961579102316 1104 64-65 0.44300073735285395 1104 66-67 0.33389149879916147 1104 68-69 0.37846716857566776 1104 70-71 0.4002535857413889 1104 72-73 0.3493352072254652 1104 74-75 0.42494549677454074 1104 76-77 0.43080711986451803 1104 78-79 0.41973672346723845 1104 80-81 0.3184934556455232 1104 82-83 0.3525756164296894 1104 84-85 0.3426831960314374 1104 86-87 0.3929643237081528 1104 88-89 0.38981238294676146 1104 90-91 0.3779322055436971 1104 92-93 0.3444191685975788 1104 94-95 0.32981437903784183 1104 96-97 0.36968440926568036 1104 98-99 0.33336289777890826 1104 100-101 0.31981142252292827 1104 102-103 0.392715049057454 1104 104-105 0.42023326139038986 1104 106-107 0.39152212676156495 1104 108-109 0.34789521115478905 1104 110-111 0.3376416130048412 1104 112-113 0.3299140805542713 1104 114-115 0.3492759960102063 1104 116-117 0.3885632166766797 1104 118-119 0.43122732108116324 1104 120-121 0.43101680534050857 1104 122-123 0.48482787348649836 1104 124-125 0.5765901976491854 1105 1 0.20266641261898144 1105 2 0.1846127622794853 1105 3 0.21953086620776574 1105 4 0.17644848540687263 1105 5 0.13876727906773567 1105 6 0.17777066599735747 1105 7 0.04262767106016696 1105 8 0.0973883294688207 1105 9 0.17504630655552944 1105 10-11 0.18930395056470672 1105 12-13 0.16953546955653565 1105 14-15 0.261045588177538 1105 16-17 0.3218413537721787 1105 18-19 0.2844860472770989 1105 20-21 0.2527996096201832 1105 22-23 0.22953803680015739 1105 24-25 0.33306798718739117 1105 26-27 0.3215190096898084 1105 28-29 0.31662204855954457 1105 30-31 0.2881293220714838 1105 32-33 -0.07336321853110661 1105 34-35 0.27700477675143986 1105 36-37 0.3362496778832025 1105 38-39 0.30936332599890903 1105 40-41 0.41291287659292664 1105 42-43 0.3519001257868055 1105 44-45 0.3218998493085721 1105 46-47 0.2830248485764386 1105 48-49 0.35744742399590734 1105 50-51 0.3838043211434652 1105 52-53 0.36963341983045694 1105 54-55 0.3050777771757822 1105 56-57 0.28223609249195647 1105 58-59 0.3043363080812256 1105 60-61 0.264700441602713 1105 62-63 0.3747269962841173 1105 64-65 0.3743754563340147 1105 66-67 0.3594667126773956 1105 68-69 0.3927308492246979 1105 70-71 0.3539025719141762 1105 72-73 0.352671843604881 1105 74-75 0.35424921386103847 1105 76-77 0.4412769961642482 1105 78-79 0.36718170135762307 1105 80-81 0.34896075668898163 1105 82-83 0.40950717459568864 1105 84-85 0.39130465415433235 1105 86-87 0.25543374253999573 1105 88-89 0.28279748884817124 1105 90-91 0.269320872496678 1105 92-93 0.3436216989068228 1105 94-95 0.2936933108023503 1105 96-97 0.344225961665348 1105 98-99 0.2727737959115295 1105 100-101 0.2547490125469274 1105 102-103 0.27187894652386646 1105 104-105 0.3987462668344186 1105 106-107 0.369856883683763 1105 108-109 0.3574493519189339 1105 110-111 0.3307767955641161 1105 112-113 0.3123141634103419 1105 114-115 0.32624184827207614 1105 116-117 0.4145633078503508 1105 118-119 0.44260233597686494 1105 120-121 0.43617871458112134 1105 122-123 0.4044489869089425 1105 124-125 0.3688417953877625 1106 1 0.07389439098981754 1106 2 0.12388510042822531 1106 3 0.18107050367035882 1106 4 0.1259788997045419 1106 5 -0.0014963631220723528 1106 6 0.020692876656326575 1106 7 0.05956127250420451 1106 8 0.05324452962678805 1106 9 0.08405937303248123 1106 10-11 0.09698410704100979 1106 12-13 0.08817906067203296 1106 14-15 0.057888770855392124 1106 16-17 0.09016626944861628 1106 18-19 0.1804094123015858 1106 20-21 0.10979606420893617 1106 22-23 0.15309209109256727 1106 24-25 0.16839862765446867 1106 26-27 0.19350487116202686 1106 28-29 0.14910729561140812 1106 30-31 0.24693630784476284 1106 32-33 0.24102024444785997 1106 34-35 0.31435977060723985 1106 36-37 0.27946341973257915 1106 38-39 0.3114332954444805 1106 40-41 0.3362820658128385 1106 42-43 0.2287258949881732 1106 44-45 0.1791285979097026 1106 46-47 0.23554196317220288 1106 48-49 0.239456127794341 1106 50-51 0.1150698408921329 1106 52-53 0.13930908035683842 1106 54-55 0.18904351270651887 1106 56-57 0.2810023030960451 1106 58-59 0.2681310250460811 1106 60-61 0.19855456233483437 1106 62-63 0.21079268732572842 1106 64-65 0.2940320570901349 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70-71 -1.647415446168317 2216 72-73 -1.6931466794196837 2216 74-75 -1.8384860811921158 2216 76-77 -1.8630947286701591 2216 78-79 -1.8516421016911124 2216 80-81 -1.727455963652627 2216 82-83 -1.576567656228839 2216 84-85 -1.5436318702538543 2216 86-87 -1.6715409984286964 2216 88-89 -1.633356780894264 2216 90-91 -1.6523678804156816 2216 92-93 -1.780385314619263 2216 94-95 -1.7895793897232686 2216 96-97 -1.6490467786194891 2216 98-99 -1.7225713936206652 2216 100-101 -1.6727515440821286 2216 102-103 -1.9000573075216387 2216 104-105 -1.7372496419420322 2216 106-107 -1.7581509011711525 2216 108-109 -1.7497822534787737 2216 110-111 -1.6343066590348307 2216 112-113 -1.6912721180690191 2216 114-115 -1.797530585866781 2216 116-117 -1.899554395339134 2216 118-119 -1.987177914824283 2216 120-121 -1.9931875433607829 2216 122-123 -2.1045445482421847 2216 124-125 -2.3144606251534405 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 38114.0 3 6633.0 4 3933.0 5 3330.0 6 3771.0 7 4384.0 8 5028.0 9 5467.0 10 6160.0 11 6720.0 12 7299.0 13 7346.0 14 7524.0 15 6869.0 16 5982.0 17 5727.0 18 5571.0 19 5518.0 20 5615.0 21 6271.0 22 7514.0 23 9654.0 24 12799.0 25 16235.0 26 20102.0 27 23599.0 28 28000.0 29 35857.0 30 42594.0 31 51005.0 32 65780.0 33 86759.0 34 119781.0 35 179587.0 36 335598.0 37 656406.0 38 314152.0 39 1427.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.05444529699449 18.17966831397993 12.70164816840377 24.06423822062181 2 15.685310628009411 20.080704889865622 40.03226516574725 24.20171931637772 3 19.251368571862965 24.758768513846153 29.22634299129841 26.763519922992472 4 12.58822432085811 15.999533636469629 36.97810423224904 34.43413781042322 5 13.639993097240296 38.563852859281646 33.849454542062524 13.946699501415532 6 32.57325235509833 35.81322177825441 17.63675344106164 13.976772425585612 7 29.216572012562477 32.61655987941518 20.436513306795963 17.73035480122638 8 26.545937199706728 35.44668238206336 19.740635283476852 18.26674513475307 9 27.290293402310233 16.06905406774068 19.37412983482789 37.266522695121196 10-11 25.31143217253291 26.54866492391 27.468747082438615 20.671155821118475 12-13 25.491535757752875 24.186943951268226 27.679316306032042 22.642203984946857 14-15 22.663217368778486 25.77169288646489 25.14440142546086 26.420688319295767 16-17 22.79745541610854 29.263228149109256 25.63112868070886 22.30818775407334 18-19 22.662758544718574 28.101789788533853 27.25896387935638 21.976487787391196 20-21 23.551833395258694 27.309178085516745 26.950298828770464 22.188689690454098 22-23 23.232252501812432 27.391677601957287 26.731405454434267 22.64466444179601 24-25 22.894566095159853 27.587884926877315 26.897082094394314 22.62046688356852 26-27 22.75537477857712 27.82343496125202 26.762705862883422 22.65848439728744 28-29 22.6154034643501 27.749432623699704 27.012619382391208 22.622544529558994 30-31 22.724215445480965 27.502723547977855 27.000489165086684 22.7725718414545 32-33 22.539812863902974 27.706126499371265 26.875281810382575 22.87877882634319 34-35 22.964232553515345 27.712897966461338 26.6386003137569 22.684269166266418 36-37 23.079770640340037 27.488030275251184 26.627838246483726 22.804360837925053 38-39 22.68840156268144 27.55396609464417 26.829964421341973 22.92766792133241 40-41 22.898558159219235 27.371750553500373 26.990765083832425 22.738926203447964 42-43 22.75558499133057 27.43775441313136 26.795761714810094 23.010898880727975 44-45 22.745839942106333 27.60691169793051 26.76588630088522 22.881362059077944 46-47 22.735873589691263 27.738806448791582 26.58168566514096 22.9436342963762 48-49 22.801457705613537 27.689898626338955 26.5660153426245 22.942628325423005 50-51 22.72013515527394 27.62140291230323 26.621638230620814 23.036823701802017 52-53 22.99465440186836 27.487303009193237 26.674023928670632 22.844018660267775 54-55 22.71886078175042 27.399158729129713 26.72198651485423 23.159993974265635 56-57 22.64270730916305 27.75748704663816 26.59377702381488 23.006028620383905 58-59 22.69531016804967 27.434299631365295 26.667787557459256 23.202602643125783 60-61 22.75652087126526 27.450503499686597 26.963583485061037 22.829392143987107 62-63 22.60285506114723 27.567143474192594 26.815502261827127 23.014499202833044 64-65 22.458721801442508 27.4449439381483 26.76168319660422 23.334651063804976 66-67 22.548896811429916 27.800048748118005 26.4655165376241 23.185537902827974 68-69 22.461941189451256 27.554947400440128 26.81786775487658 23.165243655232036 70-71 22.452402312250907 27.42784246052906 26.905178772114713 23.214576455105316 72-73 22.608909666913952 27.35845359237434 26.852899047725693 23.179737692986016 74-75 22.34803529141731 27.612473664862165 26.73273077418307 23.306760269537453 76-77 22.518654671073655 27.383704249696184 26.746448089980944 23.351192989249217 78-79 22.672347549069695 27.445576484058208 26.82073074175733 23.061345225114763 80-81 22.49807670865017 27.651891030440957 26.724232444630434 23.125799816278438 82-83 22.46325813233767 27.553339070234745 26.86218163521285 23.121221162214738 84-85 22.574031045815286 27.383293612647858 26.738626537461112 23.304048804075748 86-87 22.516940289386696 27.183158511474986 26.9499408967461 23.349960302392212 88-89 22.55073310239735 27.146609516580906 26.91519925284411 23.387458128177634 90-91 22.49307754433042 27.42787364410707 26.97137521144012 23.107673600122393 92-93 22.60659225230545 27.030291411937174 26.950461789760073 23.412654545997302 94-95 22.49474832048864 26.98628686669689 27.019331404297237 23.499633408517234 96-97 22.561695469738993 26.90182471922688 26.997450219826725 23.5390295912074 98-99 22.407161848537477 26.812063243292002 26.972541942978665 23.808232965191856 100-101 22.288709143970458 26.86967954743624 27.13744006679096 23.704171241802342 102-103 22.43907668536151 26.71904951122681 27.186556494117127 23.655317309294556 104-105 22.392346705591628 26.746303414704865 27.1682209381378 23.693128941565718 106-107 22.345999719391404 26.84739040999153 27.08017435023029 23.726435520386772 108-109 22.378213423935136 26.98541861688043 26.75384044485386 23.882527514330576 110-111 22.223390556589052 26.98449817176396 27.077384639739694 23.714726631907297 112-113 22.24500339930186 27.07763091508833 27.045246619583153 23.632119066026657 114-115 22.143001684139065 27.189637229358105 26.949791953806475 23.717569132696354 116-117 22.080526320819878 27.17024931562861 27.045462121736634 23.70376224181488 118-119 22.109306401452475 27.308269519375074 26.803051875588146 23.77937220358431 120-121 22.171582807298044 27.32175356082436 26.991953455788316 23.51471017608928 122-123 22.217860327414083 27.32736162996031 26.9061175963214 23.548660446304208 124-125 22.18779506906802 27.42435157661596 26.730640554875563 23.65721279944046 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 9501.0 1 5131.0 2 858.0 3 941.5 4 851.0 5 726.5 6 669.5 7 636.5 8 648.5 9 654.0 10 586.5 11 527.0 12 531.0 13 571.5 14 621.0 15 665.0 16 727.0 17 824.5 18 984.5 19 1273.0 20 1655.0 21 2194.5 22 2951.5 23 3990.5 24 5361.0 25 7032.5 26 9655.0 27 12935.5 28 15947.0 29 19153.0 30 23452.5 31 28253.5 32 32856.0 33 37224.0 34 42177.0 35 46499.0 36 50984.0 37 56407.5 38 59416.5 39 60405.5 40 61488.5 41 65389.5 42 70152.5 43 73856.5 44 76202.0 45 77648.0 46 77557.5 47 77701.0 48 81151.0 49 82566.5 50 81526.5 51 79428.5 52 77367.5 53 77130.5 54 74531.0 55 68448.5 56 62024.0 57 56380.5 58 51981.5 59 46831.0 60 40237.5 61 33083.5 62 27025.5 63 21390.0 64 16532.0 65 13346.0 66 10397.5 67 8670.0 68 6522.0 69 3968.5 70 2810.0 71 2243.5 72 1740.0 73 1451.0 74 1101.5 75 847.0 76 633.0 77 358.0 78 204.5 79 133.0 80 79.5 81 56.5 82 43.5 83 26.0 84 16.5 85 17.0 86 20.0 87 14.5 88 6.0 89 8.0 90 6.0 91 4.0 92 4.5 93 4.0 94 3.5 95 5.0 96 4.5 97 1.0 98 1.5 99 2.0 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4742559691677912 2 0.46436789933294986 3 0.45981845875166133 4 0.45777585277638894 5 0.46613196812977603 6 0.5251818499603781 7 0.5209109465575358 8 0.5298705591308898 9 0.5365554514135994 10-11 0.552942722078853 12-13 0.5617166431999093 14-15 0.5406406633641442 16-17 0.5348378054798476 18-19 0.5447722981777634 20-21 0.5453293725346559 22-23 0.5554031338217946 24-25 0.5487646644021594 26-27 0.5570511454609349 28-29 0.5372750057912522 30-31 0.5424511550240447 32-33 0.5437509951901272 34-35 0.5425904236132678 36-37 0.4910610456007142 38-39 0.4571723555564221 40-41 0.45510653814961255 42-43 0.4681049398104369 44-45 0.47260795752865103 46-47 0.47808585537142706 48-49 0.4923376743352594 50-51 0.4738149519685847 52-53 0.4733971462009154 54-55 0.46302163630379306 56-57 0.4452184683147712 58-59 0.4634394420714625 60-61 0.4604683788347026 62-63 0.47671638091073304 64-65 0.4769252837945677 66-67 0.48458505620183917 68-69 0.49449633746821775 70-71 0.49078250842226795 72-73 0.5023418013277867 74-75 0.4914788513683835 76-77 0.48957551398233423 78-79 0.5005313096678862 80-81 0.494403491742069 82-83 0.49568012047661425 84-85 0.49215198288296197 86-87 0.4830298902888477 88-89 0.4881596166585659 90-91 0.4727008032547998 92-93 0.4721205174663701 94-95 0.46585343095132986 96-97 0.4553386524649844 98-99 0.4162506017563626 100-101 0.41365092142419774 102-103 0.403345045821687 104-105 0.4078944864029755 106-107 0.4073142006145458 108-109 0.41998764223384955 110-111 0.44368651383331686 112-113 0.4434776109494822 114-115 0.4361427985837313 116-117 0.4298525006371538 118-119 0.4727472261178742 120-121 0.4681745741050484 122-123 0.430502420720195 124-125 0.44036727912349927 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2154111.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 68.00960096321694 #Duplication Level Percentage of deduplicated Percentage of total 1 82.09551021151032 55.83282890356518 2 10.69138010012004 14.542329887104847 3 3.345042594487653 6.824850361682076 4 1.4602430407437628 3.9724218604119144 5 0.7608951745245941 2.587408859712748 6 0.4329814209199555 1.7668136196751707 7 0.27139734171136964 1.292033743858766 8 0.18181724538217062 0.9892254645338177 9 0.12723570399565112 0.7787924511316398 >10 0.5796426912980702 6.889745846756437 >50 0.038746967413627154 1.8167533748188702 >100 0.014667483717054498 1.6294643569560734 >500 1.4667472524225258E-4 0.05247002295470533 >1k 1.4667472524225258E-4 0.13376863266589323 >5k 1.4667472524225258E-4 0.8910926141718302 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 8933 0.4146954358433711 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 4.6422863074372675E-5 5 0.0 0.0 0.0 0.0 4.6422863074372675E-5 6 0.0 0.0 0.0 0.0 4.6422863074372675E-5 7 0.0 0.0 0.0 0.0 4.6422863074372675E-5 8 4.6422863074372675E-5 0.0 0.0 0.0 4.6422863074372675E-5 9 4.6422863074372675E-5 0.0 0.0 0.0 4.6422863074372675E-5 10-11 4.6422863074372675E-5 0.0 0.0 0.0 4.6422863074372675E-5 12-13 9.284572614874535E-5 0.0 0.0 2.3211431537186338E-5 4.6422863074372675E-5 14-15 9.284572614874535E-5 0.0 0.0 6.963429461155902E-5 6.963429461155902E-5 16-17 9.284572614874535E-5 0.0 0.0 1.3926858922311803E-4 9.284572614874535E-5 18-19 9.284572614874535E-5 0.0 0.0 1.6248002076030437E-4 9.284572614874535E-5 20-21 9.284572614874535E-5 0.0 0.0 3.713829045949814E-4 9.284572614874535E-5 22-23 9.284572614874535E-5 0.0 0.0 5.106514938180995E-4 1.3926858922311803E-4 24-25 9.284572614874535E-5 0.0 0.0 8.124001038015219E-4 2.0890288383467704E-4 26-27 9.284572614874535E-5 0.0 0.0 0.001299840166082435 2.3211431537186337E-4 28-29 1.6248002076030437E-4 0.0 0.0 0.003110331825982969 2.3211431537186337E-4 30-31 1.856914522974907E-4 0.0 0.0 0.008913189710279554 2.3211431537186337E-4 32-33 1.856914522974907E-4 0.0 0.0 0.022584722885682307 2.3211431537186337E-4 34-35 2.5532574690904973E-4 0.0 0.0 0.04470521714062089 2.3211431537186337E-4 36-37 2.7853717844623607E-4 0.0 0.0 0.08126322181168938 2.7853717844623607E-4 38-39 2.7853717844623607E-4 0.0 0.0 0.13516016584103605 2.7853717844623607E-4 40-41 2.7853717844623607E-4 0.0 0.0 0.19274772748479535 2.7853717844623607E-4 42-43 2.7853717844623607E-4 0.0 0.0 0.2487337003524888 2.7853717844623607E-4 44-45 2.7853717844623607E-4 0.0 0.0 0.31929645222553527 2.7853717844623607E-4 46-47 2.7853717844623607E-4 0.0 0.0 0.4104245324405288 2.7853717844623607E-4 48-49 3.2496004152060873E-4 0.0 0.0 0.5071929905190586 2.7853717844623607E-4 50-51 3.2496004152060873E-4 0.0 0.0 0.6169598502584128 2.7853717844623607E-4 52-53 3.2496004152060873E-4 0.0 0.0 0.725728618441668 3.017486099834224E-4 54-55 3.4817147305779507E-4 0.0 0.0 0.844942530816657 3.4817147305779507E-4 56-57 3.713829045949814E-4 0.0 0.0 0.9874607204549812 3.713829045949814E-4 58-59 3.713829045949814E-4 0.0 0.0 1.1493836668583932 3.713829045949814E-4 60-61 4.1780576766935407E-4 0.0 0.0 1.3276474610639841 3.713829045949814E-4 62-63 4.1780576766935407E-4 0.0 0.0 1.5054006037757572 3.713829045949814E-4 64-65 4.1780576766935407E-4 0.0 0.0 1.6901403873802232 3.713829045949814E-4 66-67 4.1780576766935407E-4 0.0 0.0 1.9053799920245522 3.713829045949814E-4 68-69 4.1780576766935407E-4 0.0 0.0 2.163328630697304 3.713829045949814E-4 70-71 5.570743568924721E-4 0.0 0.0 2.4512896503476376 3.713829045949814E-4 72-73 5.570743568924721E-4 0.0 0.0 2.744171493483855 4.1780576766935407E-4 74-75 5.570743568924721E-4 0.0 0.0 3.038515656806915 4.1780576766935407E-4 76-77 5.570743568924721E-4 0.0 0.0 3.3634060640329118 4.1780576766935407E-4 78-79 5.570743568924721E-4 0.0 0.0 3.725898990349151 4.1780576766935407E-4 80-81 5.570743568924721E-4 0.0 0.0 4.14119792341249 4.410171992065404E-4 82-83 5.570743568924721E-4 0.0 0.0 4.572535955668022 5.106514938180995E-4 84-85 5.570743568924721E-4 0.0 0.0 4.994891163918665 5.338629253552859E-4 86-87 5.570743568924721E-4 0.0 0.0 5.429594853747091 5.570743568924721E-4 88-89 5.802857884296584E-4 0.0 0.0 5.9204005736008956 5.802857884296584E-4 90-91 6.034972199668448E-4 2.3211431537186338E-5 0.0 6.472995124206691 6.034972199668448E-4 92-93 6.034972199668448E-4 4.6422863074372675E-5 0.0 7.0496367178850114 6.034972199668448E-4 94-95 6.034972199668448E-4 4.6422863074372675E-5 0.0 7.627044288804059 6.034972199668448E-4 96-97 6.034972199668448E-4 4.6422863074372675E-5 0.0 8.202594945200131 6.034972199668448E-4 98-99 6.034972199668448E-4 4.6422863074372675E-5 0.0 8.821783092886115 6.034972199668448E-4 100-101 6.267086515040312E-4 4.6422863074372675E-5 0.0 9.513414118399655 6.034972199668448E-4 102-103 7.195543776527765E-4 4.6422863074372675E-5 0.0 10.245015229020233 6.267086515040312E-4 104-105 8.124001038015219E-4 4.6422863074372675E-5 0.0 10.965915869702165 6.499200830412175E-4 106-107 8.356115353387081E-4 4.6422863074372675E-5 0.0 11.682174224076661 6.499200830412175E-4 108-109 8.356115353387081E-4 4.6422863074372675E-5 0.0 12.426030970548872 6.963429461155901E-4 110-111 8.588229668758944E-4 4.6422863074372675E-5 0.0 13.22921613603013 7.891886722643355E-4 112-113 8.820343984130808E-4 4.6422863074372675E-5 0.0 14.091265491889693 7.891886722643355E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2625 0.0 65.9693 1 GTATCAA 4720 0.0 48.16145 1 ATCAACG 5545 0.0 39.176006 3 GTACATG 11680 0.0 38.77216 1 CAACGCA 5645 0.0 38.487404 5 TCAACGC 5780 0.0 37.484615 4 AACGCAG 5875 0.0 36.67928 6 TACATGG 12180 0.0 36.45022 2 TATCAAC 6180 0.0 36.30458 2 ACATGGG 12300 0.0 34.693085 3 ACGCAGA 7055 0.0 30.123924 7 CATGGGA 7655 0.0 29.70282 4 TGGTATC 1390 0.0 29.542204 2 CGCAGAG 7170 0.0 29.059572 8 ATGGGAG 3410 0.0 28.103548 5 CATGGGG 5400 0.0 27.887287 4 GTGGTAT 1750 0.0 25.503595 1 GCAGAGT 7990 0.0 25.108654 9 GAGTACA 8670 0.0 24.434816 1 AGTACAT 8570 0.0 23.33281 2 >>END_MODULE