FastQCFastQC Report
Thu 26 May 2016
SRR1364112_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364112_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2154111
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82170.38145666588212024No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78540.36460516658612296No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45000.20890288383467706No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23920.11104348847389946No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA51200.074.7119451
GTATCAA92350.051.357181
GTGGTAT21050.039.9109461
ATCAACG118200.039.864573
TGGTATC20850.039.093452
TCAACGC120750.038.9734344
TATCAAC121800.038.7848972
CAACGCA123350.038.1037145
AACGCAG128650.036.950166
GTACATG113100.034.4540371
TACATGG116500.032.735692
ACGCAGA147900.032.063427
CGCAGAG149150.031.5952368
ACATGGG119900.030.6654013
GTATACG1454.024956E-628.7643811
GCAGAGT161150.028.7255929
GAGTACT104650.027.09214212-13
CATGGGG52250.026.5307164
CAGAGTA160100.025.94082310-11
CATGGGA74050.025.3887544
Produced by FastQC (version 0.11.5)