FastQCFastQC Report
Thu 26 May 2016
SRR1364111_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364111_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2337812
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN96030.41076870167489943No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA24600.064.596931
GTATCAA43450.047.3938521
TCAACGC48600.040.6703224
ATCAACG49300.040.20673
CAACGCA50000.039.5315555
TATCAAC54400.036.9812472
AACGCAG53500.036.9453776
GTACATG99300.034.762781
TACATGG97350.034.666572
ACATGGG100400.031.9562763
ACGCAGA63500.030.8492557
CGCAGAG65350.029.7026048
CATGGGG52750.026.8615024
CATGGGA50200.025.3796634
TAAGGTG14650.024.7895135
GAGTACA69650.024.267941
GCAGAGT78950.023.2284979
TAAGGTA10000.022.0281244
GTACCGT3307.3305273E-1021.6492636
CAGAGTA72900.021.64416510-11
Produced by FastQC (version 0.11.5)