FastQCFastQC Report
Thu 26 May 2016
SRR1364111_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364111_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2337812
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72940.3120011361050418No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70880.3031894780247514No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38510.16472667605436195No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA44950.076.5019151
GTATCAA83500.052.60261
TGGTATC17700.050.422962
GTGGTAT20950.044.6623761
ATCAACG106500.040.8934023
TCAACGC107700.040.438634
CAACGCA108900.039.9930235
TATCAAC110600.039.432942
AACGCAG114650.038.2467426
ACGCAGA132550.032.908567
CGCAGAG134900.032.2911728
TACATGG94000.031.7750472
GTACATG95450.031.7185971
ACATGGG94500.029.8427123
GCAGAGT153550.028.136599
GAGTACT104200.026.24121312-13
CAGAGTA146250.025.87807510-11
AGAGTAC140100.025.59113910-11
GTACTTT112650.024.10971314-15
CATGGGG50400.023.96444
Produced by FastQC (version 0.11.5)