FastQCFastQC Report
Thu 26 May 2016
SRR1364110_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364110_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2873318
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN119940.4174268215352425No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA42800.045.605571
GGTATCA30850.042.8238451
TCAACGC49100.038.0684624
ATCAACG49750.037.6821563
CAACGCA50250.037.435485
TATCAAC54150.035.6075442
AACGCAG53650.035.1752436
TACATGG100250.032.055672
GTACATG103700.031.5051441
ACATGGG105700.029.334663
ACGCAGA65300.027.8978717
CGCAGAG66900.026.8746998
GAGTACA75300.025.1315921
CATGGGG72100.025.0163844
TGGTATC18250.023.8043022
AGTACAT77650.022.5320842
GTGGTAT20100.022.2051181
GTATATA15550.020.2830541
ATGGGGA41550.020.2013535
GCAGAGT85300.019.472339
Produced by FastQC (version 0.11.5)