##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364110_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2873318 Sequences flagged as poor quality 0 Sequence length 125 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.189258550567672 31.0 31.0 34.0 28.0 34.0 2 31.23863073979281 33.0 31.0 34.0 28.0 34.0 3 31.314242280179222 33.0 31.0 34.0 28.0 34.0 4 34.7969713063434 37.0 35.0 37.0 32.0 37.0 5 34.83649147083616 37.0 35.0 37.0 32.0 37.0 6 34.91088977969024 37.0 35.0 37.0 32.0 37.0 7 34.8368513335454 37.0 35.0 37.0 32.0 37.0 8 34.81002868460783 37.0 35.0 37.0 32.0 37.0 9 36.38385100430931 39.0 37.0 39.0 32.0 39.0 10-11 36.301705902374884 39.0 37.0 39.0 32.0 39.0 12-13 36.21800580374327 39.0 37.0 39.0 32.0 39.0 14-15 37.50740746412336 40.0 38.0 41.0 32.0 41.0 16-17 37.5067025299671 40.0 38.0 41.0 32.0 41.0 18-19 37.500773321992206 40.0 38.0 41.0 32.0 41.0 20-21 37.510795881277325 40.0 38.0 41.0 32.0 41.0 22-23 37.46432138732991 40.0 38.0 41.0 32.0 41.0 24-25 37.46054770129864 40.0 38.0 41.0 32.0 41.0 26-27 37.41497425624313 40.0 38.0 41.0 31.0 41.0 28-29 37.36601274206336 40.0 38.0 41.0 31.0 41.0 30-31 37.31413856732878 40.0 37.0 41.0 31.0 41.0 32-33 37.221439812787864 40.0 37.0 41.0 31.0 41.0 34-35 37.19785558020379 40.0 37.0 41.0 31.0 41.0 36-37 37.14058224672661 40.0 37.0 41.0 31.0 41.0 38-39 37.06146274098447 40.0 37.0 41.0 30.0 41.0 40-41 36.98468042868906 40.0 37.0 41.0 30.0 41.0 42-43 36.8736836646692 40.0 37.0 41.0 30.0 41.0 44-45 36.77740403255052 40.0 36.5 41.0 30.0 41.0 46-47 36.66789300731767 40.0 36.0 41.0 30.0 41.0 48-49 36.54986830556172 40.0 36.0 41.0 30.0 41.0 50-51 36.420926956222736 40.0 36.0 41.0 30.0 41.0 52-53 36.270323194300104 39.5 35.0 41.0 29.5 41.0 54-55 36.08084381888813 39.0 35.0 41.0 29.0 41.0 56-57 35.877964778002294 39.0 35.0 41.0 29.0 41.0 58-59 35.6540492559473 39.0 35.0 41.0 28.0 41.0 60-61 35.402425001339914 38.0 35.0 40.5 28.0 41.0 62-63 35.124139757590356 38.0 34.0 40.0 28.0 41.0 64-65 34.816477326909165 37.0 34.0 40.0 28.0 41.0 66-67 34.49869575870126 37.0 34.0 39.5 27.5 41.0 68-69 34.16340116200156 36.0 34.0 39.0 27.0 41.0 70-71 33.81449512375588 35.5 34.0 39.0 26.0 41.0 72-73 33.471607389088156 35.0 33.0 38.5 26.0 40.0 74-75 33.19829775193696 35.0 33.0 37.0 26.0 39.5 76-77 32.97330229372454 35.0 33.0 37.0 26.0 39.0 78-79 32.68607982130763 35.0 33.0 36.5 26.0 39.0 80-81 32.50242402685676 35.0 33.0 36.0 26.0 38.0 82-83 32.42291385777697 35.0 33.0 36.0 26.0 37.0 84-85 32.233941909666804 35.0 33.0 35.0 26.0 37.0 86-87 32.06232028616394 35.0 33.0 35.0 26.0 36.0 88-89 31.89847242804312 35.0 33.0 35.0 26.0 36.0 90-91 31.76891628424003 35.0 33.0 35.0 25.5 36.0 92-93 31.65932294998326 35.0 33.0 35.0 25.0 36.0 94-95 31.56479912769836 35.0 33.0 35.0 25.0 35.0 96-97 31.4840419682054 35.0 33.0 35.0 25.0 35.0 98-99 31.409267787275894 35.0 33.0 35.0 25.0 35.0 100-101 31.33516338950301 35.0 33.0 35.0 24.5 35.0 102-103 30.228289211288136 34.0 30.5 35.0 21.5 35.0 104-105 31.05540667618412 35.0 32.5 35.0 23.5 35.0 106-107 31.14588900358401 35.0 33.0 35.0 23.0 35.0 108-109 31.116473533385445 35.0 33.0 35.0 23.0 35.0 110-111 31.032067978553016 35.0 33.0 35.0 23.0 35.0 112-113 30.944130096285896 35.0 33.0 35.0 20.0 35.0 114-115 30.83640359333704 35.0 33.0 35.0 19.0 35.0 116-117 30.727050747602597 35.0 33.0 35.0 18.5 35.0 118-119 30.58276494282916 35.0 33.0 35.0 13.5 35.0 120-121 30.425401052024178 35.0 33.0 35.0 2.0 35.0 122-123 30.25217501160679 35.0 32.5 35.0 2.0 35.0 124-125 29.38973844872026 34.5 30.5 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.36141483396983887 1101 2 0.4200905517151128 1101 3 0.4436379198969327 1101 4 0.4644017673019363 1101 5 0.3701978338609493 1101 6 0.351575813698652 1101 7 0.4197389960375233 1101 8 0.4434294694530436 1101 9 0.4287099271567598 1101 10-11 0.49920472715846387 1101 12-13 0.5238508259262176 1101 14-15 0.5879523963439226 1101 16-17 0.5813795596579965 1101 18-19 0.5294905844631046 1101 20-21 0.5410066201503767 1101 22-23 0.6434773898460975 1101 24-25 0.6408492230053966 1101 26-27 0.635811244779596 1101 28-29 0.6950482392868906 1101 30-31 0.6556166168042452 1101 32-33 0.6538296019558274 1101 34-35 0.6472765354941288 1101 36-37 0.6795125176605978 1101 38-39 0.5882163060139973 1101 40-41 0.6769211027219626 1101 42-43 0.6292948913732843 1101 44-45 0.6558420166329171 1101 46-47 0.6608454022934325 1101 48-49 0.562456490573588 1101 50-51 0.5972089749441452 1101 52-53 0.625430140568632 1101 54-55 0.6389596071073882 1101 56-57 0.6775045596019567 1101 58-59 0.5980278632870508 1101 60-61 0.5835165103094511 1101 62-63 0.5602712685219018 1101 64-65 0.5767472503516089 1101 66-67 0.577242366616737 1101 68-69 0.5310457387272223 1101 70-71 0.5796414279862816 1101 72-73 0.5773718321456087 1101 74-75 0.5499717364572589 1101 76-77 0.5035279079987234 1101 78-79 0.5329910618789455 1101 80-81 0.4715816894734459 1101 82-83 0.5081079037267884 1101 84-85 0.5900933970007074 1101 86-87 0.5499721307985936 1101 88-89 0.5614910618264055 1101 90-91 0.6139216118320405 1101 92-93 0.5920217126393759 1101 94-95 0.5204234951576794 1101 96-97 0.5133884165970883 1101 98-99 0.5028683331889106 1101 100-101 0.5614954662496388 1101 102-103 0.5634060011752666 1101 104-105 0.48664374095366725 1101 106-107 0.559484366356596 1101 108-109 0.608429641559578 1101 110-111 0.5495649317571889 1101 112-113 0.5342162214650195 1101 114-115 0.605952905989227 1101 116-117 0.6016112669579847 1101 118-119 0.5813108948725336 1101 120-121 0.6353028816223443 1101 122-123 0.6105986010617208 1101 124-125 0.680782317748541 1102 1 0.39672033553247843 1102 2 0.36361543232475313 1102 3 0.33174765750878166 1102 4 0.35406682614512164 1102 5 0.37675039407766775 1102 6 0.4038670725145579 1102 7 0.47084454020597377 1102 8 0.41585358917491533 1102 9 0.469655878408183 1102 10-11 0.5112926163982223 1102 12-13 0.4926879787986138 1102 14-15 0.5247541134464484 1102 16-17 0.5932859269967636 1102 18-19 0.6484029654008907 1102 20-21 0.6656307411731817 1102 22-23 0.6502507905774522 1102 24-25 0.570755634250915 1102 26-27 0.6923455744012941 1102 28-29 0.614519304620373 1102 30-31 0.5981286510877553 1102 32-33 0.5946858695983792 1102 34-35 0.6443876047118522 1102 36-37 0.6067111715585298 1102 38-39 0.6750068605795221 1102 40-41 0.715926735563464 1102 42-43 0.7163072737746177 1102 44-45 0.7026275950997416 1102 46-47 0.7694560451045547 1102 48-49 0.829681208588859 1102 50-51 0.7824280452500076 1102 52-53 0.7692609687777789 1102 54-55 0.8250040198336492 1102 56-57 0.8831630366137517 1102 58-59 0.9007599610053632 1102 60-61 0.877959872572454 1102 62-63 0.8591322251269347 1102 64-65 0.8138250747231979 1102 66-67 0.8153334148196123 1102 68-69 0.8341970123599225 1102 70-71 0.8454755041336028 1102 72-73 0.7399793020549552 1102 74-75 0.7342129232219463 1102 76-77 0.7257702444698779 1102 78-79 0.6847129674227403 1102 80-81 0.6830291499324019 1102 82-83 0.6096441870828571 1102 84-85 0.689195933672714 1102 86-87 0.6830843971142002 1102 88-89 0.6507856371121967 1102 90-91 0.6435512072603515 1102 92-93 0.5546774799772543 1102 94-95 0.6888331902334173 1102 96-97 0.6753560733494268 1102 98-99 0.6814768526787454 1102 100-101 0.6519639083288489 1102 102-103 0.7266331893519435 1102 104-105 0.7834474687067079 1102 106-107 0.7629120202236308 1102 108-109 0.8170779884238577 1102 110-111 0.8409418486993232 1102 112-113 0.8024611326374753 1102 114-115 0.8751656822968634 1102 116-117 0.9216935305578708 1102 118-119 0.8456039369066275 1102 120-121 0.8553116072183755 1102 122-123 0.8810324323069274 1102 124-125 0.8876774726034746 1103 1 0.34026727735331974 1103 2 0.3573770597755406 1103 3 0.3330948980509376 1103 4 0.3812325208300251 1103 5 0.3755913052473048 1103 6 0.3588482062719649 1103 7 0.31003518373822203 1103 8 0.3356552278242688 1103 9 0.39916363824441703 1103 10-11 0.4038155068356559 1103 12-13 0.4134285769005217 1103 14-15 0.5221344403684611 1103 16-17 0.5002531465810662 1103 18-19 0.49784932811839155 1103 20-21 0.4785982476859516 1103 22-23 0.499862613051377 1103 24-25 0.5177384050791147 1103 26-27 0.5700271715620957 1103 28-29 0.5538828016561439 1103 30-31 0.5534744230947766 1103 32-33 0.30717658969130923 1103 34-35 0.4986621307111392 1103 36-37 0.5024616589422664 1103 38-39 0.5626421591870852 1103 40-41 0.597193139351667 1103 42-43 0.6051542995847186 1103 44-45 0.5731362129356512 1103 46-47 0.5958273588515439 1103 48-49 0.5822486495445034 1103 50-51 0.5280656183923895 1103 52-53 0.4732776073783853 1103 54-55 0.5340967501900806 1103 56-57 0.6042300390517141 1103 58-59 0.6237424314408244 1103 60-61 0.6206444156249518 1103 62-63 0.5703588117879193 1103 64-65 0.4836897401335065 1103 66-67 0.582103756445548 1103 68-69 0.6127591618533614 1103 70-71 0.5992232169470029 1103 72-73 0.5283419968053309 1103 74-75 0.5871460122482404 1103 76-77 0.5648922898147859 1103 78-79 0.5775412997044711 1103 80-81 0.5622590232706628 1103 82-83 0.507773093175075 1103 84-85 0.4418617451254292 1103 86-87 0.44290217897149375 1103 88-89 0.4042370777205555 1103 90-91 0.34408640080009434 1103 92-93 0.3157664175851771 1103 94-95 0.3803205614348002 1103 96-97 0.37299197784960825 1103 98-99 0.3795407704036329 1103 100-101 0.42937480381028337 1103 102-103 0.4254531380298161 1103 104-105 0.37744764606377856 1103 106-107 0.40639586564068964 1103 108-109 0.42867209190474753 1103 110-111 0.39511369795323503 1103 112-113 0.2866184915399437 1103 114-115 0.28747253371202675 1103 116-117 0.3123943324254128 1103 118-119 0.3484118197321031 1103 120-121 0.4035701418310964 1103 122-123 0.38684760859382195 1103 124-125 0.39188604684296635 1104 1 0.13882969199431727 1104 2 0.16563839652081924 1104 3 0.14313881027617725 1104 4 0.20833719175747945 1104 5 0.22764720206634337 1104 6 0.17765420505597973 1104 7 0.25160735596137584 1104 8 0.27386721421238036 1104 9 0.22902429646541833 1104 10-11 0.25248965162384707 1104 12-13 0.22716962520396322 1104 14-15 0.28979284148866213 1104 16-17 0.3330600922073401 1104 18-19 0.3368012593108318 1104 20-21 0.3289973430789317 1104 22-23 0.28516630304473267 1104 24-25 0.30320099480311313 1104 26-27 0.29065315023606075 1104 28-29 0.36860036730536194 1104 30-31 0.38387458126987895 1104 32-33 -0.29199720072114843 1104 34-35 0.2792172894486953 1104 36-37 0.3364274856659861 1104 38-39 0.3469151575508178 1104 40-41 0.4451885528256767 1104 42-43 0.4296102966089279 1104 44-45 0.43212469279144017 1104 46-47 0.44193702416488634 1104 48-49 0.4136703552822141 1104 50-51 0.4194879314341762 1104 52-53 0.401177674112823 1104 54-55 0.4367793779119893 1104 56-57 0.441802423831291 1104 58-59 0.5234961972436665 1104 60-61 0.5552258623474842 1104 62-63 0.5850662672045459 1104 64-65 0.6206481957040424 1104 66-67 0.512182871603514 1104 68-69 0.5711805715303342 1104 70-71 0.499565998689512 1104 72-73 0.43187329746432823 1104 74-75 0.5104193536007386 1104 76-77 0.5266763007407391 1104 78-79 0.5046147772863208 1104 80-81 0.4606422141980957 1104 82-83 0.45920515758795233 1104 84-85 0.44532389593421584 1104 86-87 0.44505931614008887 1104 88-89 0.46109160838147645 1104 90-91 0.41637145829039923 1104 92-93 0.4278524932759673 1104 94-95 0.43095402099076807 1104 96-97 0.42137082103196377 1104 98-99 0.4083587289371877 1104 100-101 0.41375380433960274 1104 102-103 0.44473797537239435 1104 104-105 0.4667679278804755 1104 106-107 0.5256303588557927 1104 108-109 0.5274397477179242 1104 110-111 0.5458615791176342 1104 112-113 0.4910156490808326 1104 114-115 0.5068509510914154 1104 116-117 0.44553401857342934 1104 118-119 0.5036540961010054 1104 120-121 0.48544905091974755 1104 122-123 0.515243939373029 1104 124-125 0.6118774617717442 1105 1 0.10635157572912846 1105 2 0.1174931631536289 1105 3 0.1896470213158068 1105 4 0.11772749753495049 1105 5 0.17934131681855803 1105 6 0.15397686317352566 1105 7 0.10923623588222853 1105 8 0.14264004736929792 1105 9 0.11978508479842986 1105 10-11 0.181963221847532 1105 12-13 0.18457654116574673 1105 14-15 0.26376088193225655 1105 16-17 0.24257292046336687 1105 18-19 0.29843138784251266 1105 20-21 0.2883714819899339 1105 22-23 0.256105089594314 1105 24-25 0.3847834259947973 1105 26-27 0.3219614564466795 1105 28-29 0.33611208319963737 1105 30-31 0.3607765521024149 1105 32-33 0.007819625631455551 1105 34-35 0.32175216259980743 1105 36-37 0.2706344398588527 1105 38-39 0.3360744601589687 1105 40-41 0.37584184674845744 1105 42-43 0.4219366273504761 1105 44-45 0.35582903029053625 1105 46-47 0.25118299744583084 1105 48-49 0.26892784059222663 1105 50-51 0.2559682114158335 1105 52-53 0.3738465187309288 1105 54-55 0.3503321434120963 1105 56-57 0.2665355434366177 1105 58-59 0.18851058982195212 1105 60-61 0.2601902088146133 1105 62-63 0.3424074480772177 1105 64-65 0.347719206885607 1105 66-67 0.3139252327591109 1105 68-69 0.3488330306734042 1105 70-71 0.40239398017997274 1105 72-73 0.3360261485776519 1105 74-75 0.3201063569652831 1105 76-77 0.3228443975141815 1105 78-79 0.3004247118766017 1105 80-81 0.3170339221207712 1105 82-83 0.3152409910915921 1105 84-85 0.2964949261296539 1105 86-87 0.2736839225175629 1105 88-89 0.2466721869926154 1105 90-91 0.2979641590010331 1105 92-93 0.31052069732012555 1105 94-95 0.3384675975998519 1105 96-97 0.2797531379371172 1105 98-99 0.2393720152544283 1105 100-101 0.2579941802243333 1105 102-103 0.33306559946326075 1105 104-105 0.39356100114078174 1105 106-107 0.35376817986723097 1105 108-109 0.36236358550833003 1105 110-111 0.3672088078218536 1105 112-113 0.3407680113243856 1105 114-115 0.2769052371920786 1105 116-117 0.33368218027869645 1105 118-119 0.3576461936553308 1105 120-121 0.40257072845075115 1105 122-123 0.39347896494006207 1105 124-125 0.4480501645769479 1106 1 0.148357087019793 1106 2 0.20033708888836088 1106 3 0.261017986041054 1106 4 0.17619904954931798 1106 5 0.0974255822084018 1106 6 0.11617439680860997 1106 7 0.18006455044974246 1106 8 0.10504157357286914 1106 9 0.26193748071359124 1106 10-11 0.24943089600805735 1106 12-13 0.184513198730059 1106 14-15 0.2789992265500274 1106 16-17 0.2466649914368162 1106 18-19 0.24981232775447637 1106 20-21 0.2655013713900516 1106 22-23 0.23978568411543932 1106 24-25 0.2787748722721801 1106 26-27 0.2948536371471917 1106 28-29 0.3065186967484266 1106 30-31 0.30528034890786415 1106 32-33 0.3003023249169843 1106 34-35 0.38509310201301616 1106 36-37 0.3448164122026469 1106 38-39 0.39831264267994726 1106 40-41 0.4478464852102846 1106 42-43 0.3686344664756547 1106 44-45 0.3508072719144977 1106 46-47 0.35093860537111965 1106 48-49 0.3683196035817886 1106 50-51 0.2898621502429961 1106 52-53 0.28548307280267693 1106 54-55 0.3021170783863525 1106 56-57 0.43422242232537656 1106 58-59 0.3384850284059766 1106 60-61 0.2790769779763238 1106 62-63 0.3279227288163753 1106 64-65 0.3304494154917066 1106 66-67 0.3610632269984393 1106 68-69 0.3268327563557776 1106 70-71 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116-117 -2.1814905433617398 2216 118-119 -2.3199358557860137 2216 120-121 -2.224323538228262 2216 122-123 -2.267666753263331 2216 124-125 -2.56867788640821 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 48341.0 3 8058.0 4 4673.0 5 3977.0 6 4322.0 7 5078.0 8 5976.0 9 6726.0 10 7160.0 11 7799.0 12 8399.0 13 8717.0 14 8482.0 15 7912.0 16 7030.0 17 6701.0 18 6558.0 19 6636.0 20 7111.0 21 7666.0 22 9439.0 23 12016.0 24 16150.0 25 20339.0 26 25629.0 27 30294.0 28 37095.0 29 45898.0 30 55578.0 31 68383.0 32 87985.0 33 116138.0 34 161579.0 35 244988.0 36 474307.0 37 904798.0 38 384158.0 39 1222.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.04589154649284 17.635942800533066 12.68622295455046 24.631942698423632 2 16.238910389372435 19.408654337029056 39.35886910098452 24.993566172613992 3 20.06802673517824 23.210976583642534 28.394029199469102 28.326967481710124 4 13.251574754696293 15.544129493505185 36.05240744470177 35.15188830709675 5 14.502236629960036 37.15134500257528 33.98352310216887 14.362895265295819 6 34.13790268265589 34.68167624445669 17.07652610494459 14.103894967942832 7 29.883761000722448 31.149882711438654 20.296676211850606 18.669680075988293 8 26.68045689701623 34.68210276753968 19.441320727122086 19.196119608322 9 26.324684463140336 16.318410641074045 19.251676139216567 38.105228756569055 10-11 25.487575730108063 25.734780221260884 27.473838530863027 21.303805517768033 12-13 26.06462632355862 23.06361218955818 27.31949246049559 23.55226902638761 14-15 23.071861232516717 25.00043741011222 24.61282206506563 27.314879292305434 16-17 23.46315730910686 28.038073830592236 25.40148940436072 23.097279455940175 18-19 23.36502447349699 27.262499295733 26.556710028517127 22.815766202252885 20-21 23.883431849335082 26.556556910357145 26.612618470289295 22.947392770018475 22-23 23.711417959967633 26.33126237187151 26.427349990620474 23.52996967754038 24-25 23.60089485615196 26.556282409882265 26.51272947749605 23.330093256469727 26-27 23.307561358206772 26.79251780915696 26.397853451480064 23.50206738115621 28-29 23.364251832505435 26.93711255649379 26.369830179198527 23.328805431802245 30-31 23.51696231217457 26.641689472222772 26.25201934370739 23.58932887189527 32-33 23.20937776673514 26.71295243227732 26.31260038983626 23.765069411151277 34-35 23.734554557290277 26.760138776934223 26.19342022084702 23.311886444928483 36-37 23.77314936257279 26.478481366839794 26.173163352744698 23.575205917842716 38-39 23.415634968002745 26.761265121469997 26.28210171082089 23.540998199706372 40-41 23.64586092150842 26.49678032339241 26.427273418591174 23.430085336507993 42-43 23.48598849959167 26.453212038625622 26.272535794754305 23.788263667028403 44-45 23.422608109388733 26.69672371631302 26.370375214229043 23.510292960069208 46-47 23.64985348955053 26.742554614878078 26.08435043187273 23.523241463698668 48-49 23.52593475538077 26.65419078687581 26.202862632064377 23.617011825679047 50-51 23.433974100962953 26.80696506521968 26.28775689832612 23.471303935491246 52-53 23.661610218596756 26.59317629541248 26.178492535272685 23.56672095071808 54-55 23.46903934674257 26.62065644980174 26.231872681445388 23.678431522010303 56-57 23.345661914403323 26.9274179916876 26.179470089512876 23.547450004396197 58-59 23.386159319940088 26.575736527310422 26.301376137726713 23.736728015022774 60-61 23.31943482912707 26.69680631603975 26.342508094348993 23.64125076048419 62-63 23.4041876103428 26.692811764909536 26.345135229980755 23.55786539476691 64-65 23.21250597119419 26.68197087525992 26.29874785019524 23.80677530335065 66-67 23.199238289088175 26.790523378981778 26.17001576405847 23.84022256787157 68-69 23.327569225259104 26.490641019183848 26.45396828061693 23.727821474940118 70-71 23.318988303808244 26.338264644897123 26.400310438820103 23.942436612474527 72-73 23.27475072498798 26.516991766417192 26.222659151498657 23.985598357096173 74-75 23.135535140407914 26.680835205829425 26.30815470953533 23.875474944227335 76-77 23.269158735338277 26.509418091787616 26.400175431785676 23.821247741088424 78-79 23.23505615996715 26.53237081423645 26.458810718996588 23.773762306799814 80-81 23.283471209660156 26.529161087956844 26.34053424103664 23.84683346134636 82-83 23.25388941742683 26.54389235547596 26.482508114611896 23.71971011248531 84-85 23.270933324472757 26.534364307742887 26.38767517485789 23.807027192926462 86-87 23.230338989363663 26.41001344164756 26.531630013536073 23.8280175554527 88-89 23.23419134986296 26.372305040186244 26.45902360042382 23.934480009526975 90-91 23.215431261344165 26.415706956960676 26.51491401734218 23.853947764352984 92-93 23.234376139212984 26.28698174933809 26.59383163540149 23.884810476047438 94-95 23.132676180297544 26.11472618516666 26.735368128841202 24.017229505694594 96-97 23.276542645285986 25.962833443816972 26.68464822848576 24.075975682411286 98-99 23.04419086516962 26.13871701639881 26.559517491442 24.257574626989562 100-101 22.997532924915152 26.03803957874841 26.82929301295957 24.135134483376863 102-103 23.001378067738308 25.818597921348974 26.820234933728866 24.359789077183848 104-105 22.876212489533344 26.098509576203544 26.65823975665637 24.36703817760674 106-107 23.00566276032657 25.99299249135594 26.5671315274286 24.43421322088889 108-109 23.01755732501218 25.979870974596857 26.575751920037522 24.42681978035344 110-111 22.912225405053547 26.066934080432485 26.749510397883437 24.27133011663053 112-113 23.04133433317427 26.099099618766946 26.75791089179868 24.101655156260104 114-115 22.87556852681685 26.332714949541767 26.548712318002377 24.243004205639004 116-117 22.778174316326375 26.26353260618805 26.715336044864706 24.24295703262087 118-119 22.861772338521547 26.24847750909823 26.576265472756372 24.313484679623855 120-121 22.758306949092415 26.320944381280007 26.75485288494606 24.165895784681517 122-123 22.8066581843095 26.39282518421163 26.601242991568906 24.199273639909958 124-125 22.85970180246585 26.432541814023054 26.499679170482217 24.20807721302888 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 12107.0 1 6364.5 2 641.5 3 612.0 4 499.5 5 399.5 6 360.0 7 357.0 8 359.0 9 343.5 10 319.5 11 305.5 12 292.5 13 306.5 14 327.5 15 358.5 16 435.0 17 515.0 18 587.0 19 705.5 20 915.5 21 1228.5 22 1744.0 23 2597.0 24 3640.5 25 4975.5 26 7301.0 27 10693.0 28 14079.0 29 18092.0 30 23339.5 31 29808.0 32 37367.0 33 42828.5 34 47690.0 35 53601.0 36 60738.0 37 68001.0 38 72251.5 39 75571.0 40 79165.0 41 82644.5 42 85906.0 43 90043.5 44 95479.0 45 101292.5 46 105449.5 47 107532.0 48 108187.0 49 108830.5 50 109662.5 51 109783.0 52 111090.0 53 112954.5 54 114363.0 55 109756.5 56 101254.5 57 92990.5 58 86361.0 59 78146.5 60 64820.0 61 53304.0 62 43529.5 63 33059.0 64 23990.5 65 17795.5 66 12904.5 67 9735.0 68 7606.0 69 5718.5 70 4153.5 71 2748.0 72 1987.0 73 1491.0 74 1009.5 75 701.5 76 493.5 77 252.0 78 143.5 79 96.5 80 59.5 81 39.0 82 32.5 83 22.5 84 18.5 85 14.5 86 13.5 87 12.0 88 9.5 89 7.0 90 8.5 91 7.0 92 7.5 93 8.5 94 6.0 95 5.5 96 5.0 97 3.5 98 1.5 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.47474731303670537 2 0.4675430982578329 3 0.46169619930686406 4 0.4590511735909496 5 0.46757790122777915 6 0.5270213738959628 7 0.5214528987045638 8 0.5309541094998883 9 0.536348569841556 10-11 0.5556990211316672 12-13 0.5638255146141151 14-15 0.5425086955220411 16-17 0.5358613282623086 18-19 0.5476943380440313 20-21 0.5479727618036012 22-23 0.5578916082382807 24-25 0.5525145493815861 26-27 0.5596317567355927 28-29 0.5388195807077393 30-31 0.5444750633240039 32-33 0.5466850519155902 34-35 0.5442662455043263 36-37 0.49308847819837553 38-39 0.46040848941885304 40-41 0.4583725156769978 42-43 0.4700663135789355 44-45 0.4748865249164903 46-47 0.4801069704084268 48-49 0.49423697620660156 50-51 0.4759306140148776 52-53 0.4757217961952001 54-55 0.4654375185760852 56-57 0.44866248706199596 58-59 0.46613357797501004 60-61 0.4628098943451438 62-63 0.47901067685512005 64-65 0.4787496545805233 66-67 0.4860408767842612 68-69 0.49580310985418247 70-71 0.4920965935549076 72-73 0.5038599973967378 74-75 0.4930362737434562 76-77 0.49026943763272984 78-79 0.5022416592942375 80-81 0.4953854742148276 82-83 0.4969168048924623 84-85 0.4945676044210909 86-87 0.48450954610662655 88-89 0.4896255826887243 90-91 0.4747995174916247 92-93 0.4737902313631836 94-95 0.46832616508162345 96-97 0.4582159023122397 98-99 0.42127254971430245 100-101 0.4188537433030385 102-103 0.40728175579591264 104-105 0.4124151938629835 106-107 0.4112318928848112 108-109 0.4237609620654588 110-111 0.44659171035019446 112-113 0.447670602418528 114-115 0.4397355252707845 116-117 0.4332969758307295 118-119 0.47662667341380244 120-121 0.46978788981936564 122-123 0.43427145898922426 124-125 0.444242509878823 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2873318.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 68.7946945950546 #Duplication Level Percentage of deduplicated Percentage of total 1 83.65425102768681 57.54968651027745 2 9.953670148710444 13.69519395960893 3 2.9889616258308056 6.1687410661610445 4 1.2218032600337823 3.362143285170645 5 0.6282573528996824 2.1610386359915545 6 0.3676035668422039 1.5173505067777293 7 0.24423138193123725 1.176127633134132 8 0.16857369011107629 0.9277580422362294 9 0.12573111061901374 0.7784670020518868 >10 0.5838698349930399 7.393417876478023 >50 0.04373446629774291 2.050041156849009 >100 0.018954897758957503 2.130592274407343 >500 2.3842419013503122E-4 0.10579495977146194 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 1.1921209506751561E-4 0.9836470910844297 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 11994 0.4174268215352425 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 3.4802969946243335E-5 3 0.0 0.0 0.0 0.0 3.4802969946243335E-5 4 0.0 0.0 0.0 0.0 3.4802969946243335E-5 5 0.0 0.0 0.0 0.0 6.960593989248667E-5 6 1.0440890983873E-4 0.0 0.0 0.0 1.0440890983873E-4 7 1.3921187978497334E-4 0.0 0.0 0.0 1.0440890983873E-4 8 1.3921187978497334E-4 0.0 0.0 0.0 1.0440890983873E-4 9 1.3921187978497334E-4 0.0 0.0 0.0 1.3921187978497334E-4 10-11 1.7401484973121666E-4 0.0 0.0 1.7401484973121668E-5 1.3921187978497334E-4 12-13 1.7401484973121666E-4 0.0 0.0 3.4802969946243335E-5 4.3503712432804167E-4 14-15 1.7401484973121666E-4 0.0 0.0 3.4802969946243335E-5 5.916504890861367E-4 16-17 2.958252445430683E-4 0.0 0.0 6.960593989248667E-5 6.612564289786233E-4 18-19 3.3062821448931164E-4 0.0 0.0 6.960593989248667E-5 7.3086236887111E-4 20-21 3.480296994624333E-4 0.0 0.0 1.2181039481185167E-4 7.83066823790475E-4 22-23 3.480296994624333E-4 0.0 0.0 2.958252445430683E-4 8.3527127870984E-4 24-25 3.8283266940867665E-4 0.0 0.0 7.3086236887111E-4 8.700742486560833E-4 26-27 3.8283266940867665E-4 0.0 0.0 0.0011833009781722732 8.700742486560833E-4 28-29 6.264534590323799E-4 0.0 0.0 0.0031496687801350213 8.700742486560833E-4 30-31 6.960593989248666E-4 0.0 0.0 0.00897916624613078 9.222787035754483E-4 32-33 6.960593989248666E-4 0.0 0.0 0.023352792833929277 9.744831584948134E-4 34-35 8.004683087635966E-4 0.0 0.0 0.046670782697912305 0.0010788920683335434 36-37 9.048772186023267E-4 0.0 0.0 0.08243083431767734 0.0010788920683335434 38-39 9.91884643467935E-4 0.0 0.0 0.13317356449930012 0.0010788920683335434 40-41 0.0010440890983873 0.0 0.0 0.18708336494603103 0.0011136950382797867 42-43 0.0010962935533066652 0.0 0.0 0.24148040697200937 0.0011310965232529083 44-45 0.0011136950382797867 0.0 0.0 0.3109819379546573 0.0011658994931991518 46-47 0.001235505433091638 0.0 0.0 0.3980763702451312 0.001200702463145395 48-49 0.0013399143429303683 0.0 0.0 0.49108730742646656 0.0012529069180647599 50-51 0.00135731582790349 0.0 0.0 0.5923291470000884 0.0013225128579572466 52-53 0.00135731582790349 0.0 0.0 0.6931359494493823 0.00146172473774222 54-55 0.00135731582790349 0.0 0.0 0.8046272636721727 0.00156613364758095 56-57 0.0014791262227153417 0.0 0.0 0.9424644261442694 0.0016357395874734366 58-59 0.0014965277076884633 0.0 0.0 1.094884033023842 0.00167054255741968 60-61 0.0016009366175271933 0.0 0.0 1.262042697675649 0.0017053455273659233 62-63 0.0016879440423928018 0.0 0.0 1.428801128173074 0.0017053455273659233 64-65 0.0017053455273659233 0.0 0.0 1.6097591704085659 0.0017053455273659233 66-67 0.0017053455273659233 0.0 0.0 1.8229621642992528 0.0017227470123390449 68-69 0.0017053455273659233 0.0 0.0 2.0724820573288443 0.0017401484973121667 70-71 0.0017227470123390449 0.0 0.0 2.3433187694505095 0.0017401484973121667 72-73 0.00177495146725841 0.0 0.0 2.6174965666870147 0.0018271559221777751 74-75 0.0017923529522315316 0.0 0.0 2.9020630504524734 0.001931564832016505 76-77 0.0018097544372046533 0.0 0.0 3.226444131836434 0.001966367801962748 78-79 0.0018271559221777751 0.0 0.0 3.5951815984168825 0.002053375226828357 80-81 0.00198376928693587 0.0 0.0 3.994615284489917 0.0022273900765595735 82-83 0.0020533752268283565 0.0 0.0 4.413068793638574 0.002436207896237033 84-85 0.002140382651693965 0.0 0.0 4.8377172314376615 0.002436207896237033 86-87 0.0022273900765595735 0.0 0.0 5.289511985794819 0.002471010866183276 88-89 0.0022621930465058166 0.0 0.0 5.782826683297846 0.0025058138361295198 90-91 0.002366601956344547 0.0 0.0 6.321437446185907 0.0025232153211026415 92-93 0.00240140492629079 0.0 0.0 6.882670139539028 0.0025754197760220064 94-95 0.00240140492629079 0.0 0.0 7.449662724418252 0.0025754197760220064 96-97 0.00240140492629079 0.0 0.0 8.02928878738796 0.0028886465055381963 98-99 0.0025058138361295198 0.0 0.0 8.644953325737006 0.0030452598702962914 100-101 0.002592821260995128 0.0 0.0 9.322706362470147 0.0030626613552694132 102-103 0.0026972301708338585 0.0 0.0 10.035175361724669 0.0031670702651081435 104-105 0.002975653930403805 0.0 0.0 10.74837522334806 0.00323667620500063 106-107 0.0029930554153769265 0.0 0.0 11.461383668636747 0.0032714791749468733 108-109 0.0029930554153769265 0.0 0.0 12.202721731461676 0.003323683629866238 110-111 0.0030278583853231697 0.0 0.0 12.993723632399895 0.003515099964570577 112-113 0.003080062840242535 0.0 0.0 13.816866076083468 0.00354990293451682 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 4280 0.0 45.60557 1 GGTATCA 3085 0.0 42.823845 1 TCAACGC 4910 0.0 38.068462 4 ATCAACG 4975 0.0 37.682156 3 CAACGCA 5025 0.0 37.43548 5 TATCAAC 5415 0.0 35.607544 2 AACGCAG 5365 0.0 35.175243 6 TACATGG 10025 0.0 32.05567 2 GTACATG 10370 0.0 31.505144 1 ACATGGG 10570 0.0 29.33466 3 ACGCAGA 6530 0.0 27.897871 7 CGCAGAG 6690 0.0 26.874699 8 GAGTACA 7530 0.0 25.131592 1 CATGGGG 7210 0.0 25.016384 4 TGGTATC 1825 0.0 23.804302 2 AGTACAT 7765 0.0 22.532084 2 GTGGTAT 2010 0.0 22.205118 1 GTATATA 1555 0.0 20.283054 1 ATGGGGA 4155 0.0 20.201353 5 GCAGAGT 8530 0.0 19.47233 9 >>END_MODULE