FastQCFastQC Report
Thu 26 May 2016
SRR1364110_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364110_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2873318
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49470.17217029232406575No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44560.15508203408046028No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA46450.056.8288571
GTATCAA74450.052.7438281
ATCAACG93400.041.339773
TCAACGC94250.041.0300644
CAACGCA96050.040.261155
TATCAAC98950.039.6223142
AACGCAG101050.038.446316
ACGCAGA117250.032.784847
TGGTATC22000.032.4510542
CGCAGAG120950.031.4867218
GTGGTAT25000.030.746851
GTACATG100900.028.6418761
TACATGG100000.028.4974352
ACATGGG103000.026.6854763
GCAGAGT140400.026.404289
GAGTACT84250.023.59031112-13
AGAGTAC124750.023.32520310-11
CAGAGTA132000.023.17108310-11
GTACTTT85500.023.10200914-15
CATGGGG68600.022.461874
Produced by FastQC (version 0.11.5)