FastQCFastQC Report
Thu 26 May 2016
SRR1364109_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364109_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1441969
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN58570.40618071539679423No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA24650.057.2185361
GTATCAA43550.053.157771
ATCAACG53650.040.82813
CAACGCA54450.040.3417745
TCAACGC54450.040.121724
TATCAAC56100.040.1004642
GTACATG98550.038.8896981
AACGCAG56250.038.7333536
TACATGG97950.038.1571272
ACATGGG99800.035.1886443
CATGGGA70700.033.3416564
ACGCAGA67500.031.8390627
TAATGCG750.003901578531.7486485
CGCAGAG67350.031.4680088
ATGGGAG28700.030.9051555
TCGACCG755.276008E-627.78152318-19
ATGGGAT23950.027.3409565
GTACCGT1755.6780846E-727.2131276
GGTAAGG8200.026.1318243
GAGTACA67750.026.0008581
Produced by FastQC (version 0.11.5)