##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364109_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1441969 Sequences flagged as poor quality 0 Sequence length 125 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.67370172312997 31.0 31.0 34.0 27.0 34.0 2 30.693733360425917 31.0 31.0 34.0 27.0 34.0 3 30.79258638708599 31.0 31.0 34.0 27.0 34.0 4 34.14394345509508 37.0 35.0 37.0 30.0 37.0 5 34.294106877471016 37.0 35.0 37.0 30.0 37.0 6 34.234692285340394 37.0 35.0 37.0 30.0 37.0 7 34.13950161203188 37.0 35.0 37.0 30.0 37.0 8 33.998805799569894 37.0 35.0 37.0 29.0 37.0 9 35.330599340207726 39.0 35.0 39.0 28.0 39.0 10-11 35.29345360406499 39.0 35.0 39.0 27.5 39.0 12-13 35.15101156820985 39.0 35.0 39.0 27.0 39.0 14-15 36.33101751840712 40.0 36.0 41.0 27.0 41.0 16-17 36.381589687434335 40.0 36.0 41.0 27.0 41.0 18-19 36.41786439236904 40.0 36.0 41.0 27.0 41.0 20-21 36.45572477633014 40.0 36.0 41.0 27.5 41.0 22-23 36.466935142156316 40.0 36.0 41.0 28.0 41.0 24-25 36.50828207818614 40.0 36.0 41.0 29.0 41.0 26-27 36.506521638121214 40.0 36.0 41.0 29.0 41.0 28-29 36.47679735139937 40.0 36.0 41.0 29.0 41.0 30-31 36.397500917148705 40.0 36.0 41.0 29.0 41.0 32-33 36.28769307800653 40.0 36.0 41.0 29.0 41.0 34-35 36.23611776674811 40.0 36.0 41.0 29.0 41.0 36-37 36.143460781750505 40.0 35.5 41.0 29.0 41.0 38-39 36.01490808748316 40.0 35.0 41.0 28.0 41.0 40-41 35.8941537578131 40.0 35.0 41.0 28.0 41.0 42-43 35.73130871745509 40.0 35.0 41.0 27.0 41.0 44-45 35.60143317921536 40.0 35.0 41.0 27.0 41.0 46-47 35.4454169957884 39.5 35.0 41.0 26.5 41.0 48-49 35.288093572053214 39.0 35.0 41.0 26.0 41.0 50-51 35.12931762055911 39.0 35.0 41.0 26.0 41.0 52-53 34.94798501216046 39.0 34.0 41.0 25.5 41.0 54-55 34.746442884694474 39.0 34.0 41.0 25.0 41.0 56-57 34.54153140601497 38.5 34.0 41.0 24.0 41.0 58-59 34.30073704774513 38.0 34.0 40.0 24.0 41.0 60-61 34.04100365541839 38.0 33.0 40.0 23.0 41.0 62-63 33.75747987647446 37.0 33.0 40.0 22.5 41.0 64-65 33.445844882934374 36.5 33.0 40.0 22.0 41.0 66-67 33.13165192871691 36.0 33.0 39.5 21.0 41.0 68-69 32.79870198319104 36.0 32.5 39.0 20.0 41.0 70-71 32.421385965995114 35.0 32.0 39.0 18.5 40.5 72-73 32.08210994827212 35.0 32.0 38.0 18.0 40.0 74-75 31.808874531976763 35.0 32.0 37.0 17.0 39.5 76-77 31.58200141611921 35.0 32.0 37.0 15.0 39.0 78-79 31.269469038516085 35.0 32.0 36.0 12.0 39.0 80-81 31.062849825481685 35.0 32.0 36.0 10.0 38.0 82-83 30.950559269998177 35.0 32.0 36.0 7.0 37.0 84-85 30.730104461330306 35.0 32.0 35.0 4.5 37.0 86-87 30.53618073620168 35.0 32.0 35.0 2.0 36.5 88-89 30.332452362013328 35.0 32.0 35.0 2.0 36.0 90-91 30.168544192004127 35.0 32.0 35.0 2.0 36.0 92-93 30.025311570498395 35.0 32.0 35.0 2.0 36.0 94-95 29.893832322331477 35.0 31.0 35.0 2.0 35.5 96-97 29.78477692654974 35.0 31.0 35.0 2.0 35.0 98-99 29.680875247664822 35.0 31.0 35.0 2.0 35.0 100-101 29.572464109838698 35.0 31.0 35.0 2.0 35.0 102-103 28.47926619781701 33.5 29.0 34.5 2.0 35.0 104-105 29.251924625286673 34.5 30.5 35.0 2.0 35.0 106-107 29.315635079533607 35.0 31.0 35.0 2.0 35.0 108-109 29.26866042196469 35.0 31.0 35.0 2.0 35.0 110-111 29.152001880761652 35.0 31.0 35.0 2.0 35.0 112-113 29.034814201969667 35.0 31.0 35.0 2.0 35.0 114-115 28.888916474626015 35.0 30.0 35.0 2.0 35.0 116-117 28.752310209165387 35.0 30.0 35.0 2.0 35.0 118-119 28.56988118329867 35.0 30.0 35.0 2.0 35.0 120-121 28.383089026185722 35.0 30.0 35.0 2.0 35.0 122-123 28.17818621620853 35.0 30.0 35.0 2.0 35.0 124-125 27.330003973733138 34.0 27.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.48451337040259546 1101 2 0.48837410394956393 1101 3 0.5743613346253227 1101 4 0.697504416320406 1101 5 0.4982827216314405 1101 6 0.6069702719138093 1101 7 0.5996625642792139 1101 8 0.6830619944403793 1101 9 0.7607575644184479 1101 10-11 0.7394441189045011 1101 12-13 0.7603993364219122 1101 14-15 0.8322159034601455 1101 16-17 0.785063942265225 1101 18-19 0.7455347699473407 1101 20-21 0.7981862897978473 1101 22-23 0.7748334073806333 1101 24-25 0.7444517946482918 1101 26-27 0.7296148943541638 1101 28-29 0.8703830531876804 1101 30-31 0.7871781631666153 1101 32-33 0.8265559279501957 1101 34-35 0.8358534084055265 1101 36-37 0.8200699041955417 1101 38-39 0.8886917926583635 1101 40-41 0.8617110536373858 1101 42-43 0.8290046474002679 1101 44-45 0.8399873141850946 1101 46-47 0.91759445635806 1101 48-49 0.8485705075948147 1101 50-51 0.983791646021956 1101 52-53 1.0339018047554376 1101 54-55 1.0074312232825946 1101 56-57 1.022421287792909 1101 58-59 1.050462377728671 1101 60-61 1.0074377728860995 1101 62-63 1.0264814065509995 1101 64-65 1.0817900465128787 1101 66-67 1.0693478455193315 1101 68-69 1.1164520038190275 1101 70-71 1.1509885157684536 1101 72-73 1.1923899417400747 1101 74-75 1.1850567158000906 1101 76-77 1.1083479801861742 1101 78-79 1.01962252646128 1101 80-81 1.0047535451908374 1101 82-83 1.0514723432490136 1101 84-85 1.031989623763053 1101 86-87 1.034094919878921 1101 88-89 1.054451680810942 1101 90-91 1.1292105296710098 1101 92-93 1.0409026063860196 1101 94-95 1.079587022176021 1101 96-97 1.1237761296594648 1101 98-99 1.022027426430629 1101 100-101 1.0428287981898947 1101 102-103 1.077850320290132 1101 104-105 1.097996583437773 1101 106-107 1.0963894804726841 1101 108-109 1.0323310014162743 1101 110-111 1.0961837198348086 1101 112-113 1.0658110334187754 1101 114-115 1.1059717823140574 1101 116-117 1.0726625818003832 1101 118-119 1.0850226611255067 1101 120-121 1.1665641420850292 1101 122-123 1.1663675144702275 1101 124-125 1.2957609405056942 1102 1 0.4602124392540148 1102 2 0.49064939078331804 1102 3 0.3842354050802186 1102 4 0.4937989222419503 1102 5 0.5124442540273364 1102 6 0.4254363857267265 1102 7 0.4865429147049838 1102 8 0.5005245202374908 1102 9 0.5340869770930823 1102 10-11 0.43502198846824314 1102 12-13 0.534829135251556 1102 14-15 0.5435730487427151 1102 16-17 0.4916745595473131 1102 18-19 0.4935228778066474 1102 20-21 0.5772535566051218 1102 22-23 0.4867766471212107 1102 24-25 0.5539145024872241 1102 26-27 0.5873437954252836 1102 28-29 0.5365624671828257 1102 30-31 0.5516859758729424 1102 32-33 0.666485879581785 1102 34-35 0.7195876424528151 1102 36-37 0.5517073797189411 1102 38-39 0.6929348288516692 1102 40-41 0.6411227143114573 1102 42-43 0.661111399359342 1102 44-45 0.6287560890154111 1102 46-47 0.607190531046129 1102 48-49 0.6280092858961908 1102 50-51 0.5525735033389054 1102 52-53 0.5962363872288634 1102 54-55 0.6266364221326199 1102 56-57 0.557297268271995 1102 58-59 0.5933786168014379 1102 60-61 0.7033672283830086 1102 62-63 0.6943623927082854 1102 64-65 0.6607805114835017 1102 66-67 0.6168161909950669 1102 68-69 0.7156344898876412 1102 70-71 0.6499003914970913 1102 72-73 0.6435344706762365 1102 74-75 0.6573315357390435 1102 76-77 0.5738060181338795 1102 78-79 0.6245182249924959 1102 80-81 0.605055989267214 1102 82-83 0.5194286220880251 1102 84-85 0.4252632495831854 1102 86-87 0.39958193831702005 1102 88-89 0.521257700641236 1102 90-91 0.476614099388307 1102 92-93 0.4399121998951401 1102 94-95 0.46086392757841566 1102 96-97 0.5155408363740719 1102 98-99 0.5178751281763425 1102 100-101 0.4879969557758521 1102 102-103 0.5411342319442056 1102 104-105 0.44351158726725615 1102 106-107 0.413499830822996 1102 108-109 0.42272532473007374 1102 110-111 0.4917173051341308 1102 112-113 0.5078639651408352 1102 114-115 0.6015393471794219 1102 116-117 0.4782230866504307 1102 118-119 0.4380612737971248 1102 120-121 0.39909862155863607 1102 122-123 0.4664577807983399 1102 124-125 0.5781593130707634 1103 1 0.3755601350702804 1103 2 0.4615544724702829 1103 3 0.4561034155642645 1103 4 0.5273044424724205 1103 5 0.361942930709489 1103 6 0.41489148260530584 1103 7 0.5223960428966024 1103 8 0.45403222769456164 1103 9 0.5108193246186801 1103 10-11 0.4980975081862269 1103 12-13 0.4550737248399912 1103 14-15 0.626061691637247 1103 16-17 0.5594623399973457 1103 18-19 0.4422520900024054 1103 20-21 0.4446582063377065 1103 22-23 0.6554023229650738 1103 24-25 0.5734868630812642 1103 26-27 0.5343152187149443 1103 28-29 0.5217984588658737 1103 30-31 0.6350695284780343 1103 32-33 0.4853978167141193 1103 34-35 0.671968790938962 1103 36-37 0.6798814893621383 1103 38-39 0.6736696180176693 1103 40-41 0.729253189241625 1103 42-43 0.6847899575834901 1103 44-45 0.5722107905459239 1103 46-47 0.6564086204167623 1103 48-49 0.7359467449428863 1103 50-51 0.6537384531699644 1103 52-53 0.5923187120585567 1103 54-55 0.6202295183669264 1103 56-57 0.661045593533629 1103 58-59 0.7007800223004423 1103 60-61 0.7262684746445842 1103 62-63 0.5778859415429167 1103 64-65 0.528097878062681 1103 66-67 0.6461014620830454 1103 68-69 0.60531144384057 1103 70-71 0.6776146802183192 1103 72-73 0.6907843992939462 1103 74-75 0.6296499639009987 1103 76-77 0.5571000454495021 1103 78-79 0.5916695591092029 1103 80-81 0.6550672914632258 1103 82-83 0.5577376900183424 1103 84-85 0.552826033968401 1103 86-87 0.6547334746835496 1103 88-89 0.6185006283035186 1103 90-91 0.6160695852088551 1103 92-93 0.5913155253947266 1103 94-95 0.6112991307617008 1103 96-97 0.6021226359033172 1103 98-99 0.6270060670914077 1103 100-101 0.6767687960112987 1103 102-103 0.6944544693726833 1103 104-105 0.8186167159933184 1103 106-107 0.677585821773917 1103 108-109 0.5942345476880817 1103 110-111 0.5444458245556056 1103 112-113 0.5291168982208561 1103 114-115 0.5403146192557671 1103 116-117 0.497637582563339 1103 118-119 0.5959185192826553 1103 120-121 0.553985265566002 1103 122-123 0.6605128256959709 1103 124-125 0.6626360213954037 1104 1 0.22688167789841884 1104 2 0.23113338680867201 1104 3 0.2186919399110714 1104 4 0.22835482323711886 1104 5 0.30986901426882696 1104 6 0.21860232722113437 1104 7 0.2249389039252634 1104 8 0.19518245974457926 1104 9 0.3531699854789849 1104 10-11 0.27319111043268407 1104 12-13 0.38287276979485085 1104 14-15 0.29823460156485737 1104 16-17 0.29650002890188176 1104 18-19 0.38340145536494674 1104 20-21 0.3026811322212595 1104 22-23 0.3360115464753264 1104 24-25 0.4410957968067848 1104 26-27 0.39046987753786766 1104 28-29 0.4294097970935482 1104 30-31 0.32387728208633604 1104 32-33 -0.3249188531081799 1104 34-35 0.31209415394766893 1104 36-37 0.3651469357328523 1104 38-39 0.37297295685335285 1104 40-41 0.5011702708071439 1104 42-43 0.3843305243348709 1104 44-45 0.37195854004746565 1104 46-47 0.4263177649028975 1104 48-49 0.49265535039386066 1104 50-51 0.4838235974356522 1104 52-53 0.521292562986531 1104 54-55 0.5082688313104455 1104 56-57 0.47041068496248784 1104 58-59 0.5314850703326996 1104 60-61 0.5874339203930603 1104 62-63 0.5872699445367644 1104 64-65 0.5452000662929777 1104 66-67 0.5316520486304555 1104 68-69 0.6162330392921973 1104 70-71 0.6814383275414144 1104 72-73 0.5971254848133327 1104 74-75 0.5357797110149356 1104 76-77 0.5649223442957805 1104 78-79 0.5275684651273984 1104 80-81 0.601189553953084 1104 82-83 0.5080433862439406 1104 84-85 0.5997746051785526 1104 86-87 0.5595525236740606 1104 88-89 0.6102247630160846 1104 90-91 0.6589157246273132 1104 92-93 0.6385565480681592 1104 94-95 0.6423040971031142 1104 96-97 0.6555610295358711 1104 98-99 0.6033083872359768 1104 100-101 0.5889533551073889 1104 102-103 0.5657960482372424 1104 104-105 0.5847823261901866 1104 106-107 0.5646074436261763 1104 108-109 0.588267848931757 1104 110-111 0.7072247312493651 1104 112-113 0.7548970947233471 1104 114-115 0.7355566383975614 1104 116-117 0.7580832777044648 1104 118-119 0.8048047547745298 1104 120-121 0.7604277757417996 1104 122-123 0.7169809587089411 1104 124-125 0.8872421897212206 1105 1 0.2756539143346721 1105 2 0.26888670549577043 1105 3 0.34566767500779605 1105 4 0.3311026358894935 1105 5 0.3627401876660059 1105 6 0.3031249823169091 1105 7 0.14049797189876756 1105 8 0.26085493559746453 1105 9 0.16240870997819457 1105 10-11 0.2888415347534661 1105 12-13 0.24382827226845905 1105 14-15 0.5022137845533194 1105 16-17 0.41758257194117476 1105 18-19 0.36014028487144856 1105 20-21 0.36015596242902603 1105 22-23 0.3449759736559628 1105 24-25 0.380187684582296 1105 26-27 0.4337276330114932 1105 28-29 0.45833793740521145 1105 30-31 0.47547060800524577 1105 32-33 0.13422577572298167 1105 34-35 0.3903013050358197 1105 36-37 0.48612606941352965 1105 38-39 0.4580149415095036 1105 40-41 0.6200912614916163 1105 42-43 0.5044766762283786 1105 44-45 0.5911035158592597 1105 46-47 0.5822787094937425 1105 48-49 0.5801825838077335 1105 50-51 0.7238554374739294 1105 52-53 0.6902867391906042 1105 54-55 0.6703028500218267 1105 56-57 0.6496282345122353 1105 58-59 0.6265938304978675 1105 60-61 0.5561926519779732 1105 62-63 0.5059166835580271 1105 64-65 0.5241182537392177 1105 66-67 0.4958504959834471 1105 68-69 0.44933581312839266 1105 70-71 0.5145899525977953 1105 72-73 0.47557497447787256 1105 74-75 0.5189141696612403 1105 76-77 0.5558718567880021 1105 78-79 0.5825986798353533 1105 80-81 0.44240482621417243 1105 82-83 0.5221052328018025 1105 84-85 0.5129488661554689 1105 86-87 0.48679799055475925 1105 88-89 0.4053342993221314 1105 90-91 0.5140723133329104 1105 92-93 0.5086718826031422 1105 94-95 0.44495749450003075 1105 96-97 0.4769239877100979 1105 98-99 0.4317483805885587 1105 100-101 0.448554491742879 1105 102-103 0.3722607539154943 1105 104-105 0.4038695936446821 1105 106-107 0.49324562683559847 1105 108-109 0.4144837421055527 1105 110-111 0.41381357381329664 1105 112-113 0.33405136596358176 1105 114-115 0.4371217949680073 1105 116-117 0.5053560556131202 1105 118-119 0.47938646049477995 1105 120-121 0.5374370218624662 1105 122-123 0.4944652487043939 1105 124-125 0.5239988978612047 1106 1 0.1917362538821692 1106 2 0.09642150397728955 1106 3 0.21812414963435955 1106 4 0.13849418111793455 1106 5 0.05362023301058372 1106 6 0.16882913451037496 1106 7 0.15123138782904988 1106 8 0.0622480648890118 1106 9 0.25970936771257414 1106 10-11 0.1709527946918783 1106 12-13 0.156998672766683 1106 14-15 0.2424964124408433 1106 16-17 0.15644774102030112 1106 18-19 0.19739157396120532 1106 20-21 0.3099312669997616 1106 22-23 0.3118683274441665 1106 24-25 0.3930757172367336 1106 26-27 0.38865411776212255 1106 28-29 0.3644961920973486 1106 30-31 0.27466912231800933 1106 32-33 0.3820871362630669 1106 34-35 0.48732098329558937 1106 36-37 0.44365938256041915 1106 38-39 0.4442336145018473 1106 40-41 0.3773912184301409 1106 42-43 0.37093715905469793 1106 44-45 0.3993508915109629 1106 46-47 0.2657654686390174 1106 48-49 0.4530042215632619 1106 50-51 0.4600581332020255 1106 52-53 0.3536726674740578 1106 54-55 0.40684812033289575 1106 56-57 0.4673225808526098 1106 58-59 0.5575189383388945 1106 60-61 0.5176505321630387 1106 62-63 0.5062755659693323 1106 64-65 0.4243189722533174 1106 66-67 0.48870469209786904 1106 68-69 0.5212377995541075 1106 70-71 0.47434619904698394 1106 72-73 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-2.683481851706585 2216 108-109 -2.746220991744675 2216 110-111 -2.7573510062974798 2216 112-113 -2.7616209466778443 2216 114-115 -2.804194316373575 2216 116-117 -2.8708872755012713 2216 118-119 -3.1476742345379023 2216 120-121 -3.196207660126692 2216 122-123 -3.1789651713831404 2216 124-125 -3.3244813948247582 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 31225.0 3 6419.0 4 4186.0 5 3878.0 6 5499.0 7 5725.0 8 5915.0 9 6439.0 10 7074.0 11 7382.0 12 8009.0 13 8069.0 14 8196.0 15 7731.0 16 6684.0 17 6009.0 18 5667.0 19 5648.0 20 5818.0 21 6037.0 22 6822.0 23 9048.0 24 11752.0 25 14010.0 26 16526.0 27 18465.0 28 21694.0 29 26798.0 30 30778.0 31 36425.0 32 46200.0 33 60256.0 34 80577.0 35 119982.0 36 215297.0 37 394298.0 38 180328.0 39 1103.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.91207062441519 18.485749778271053 12.498545611369243 23.103633985944512 2 15.496753149490278 20.593482890724548 39.71670436835677 24.193059591428398 3 19.22842475511215 26.494214718280574 28.727029028466973 25.55033149814031 4 12.302882408302048 16.472785459571256 36.92780429968352 34.29652783244317 5 12.908152033061812 40.17940993693621 33.32836365764415 13.584074372357827 6 31.835767044107925 36.623536497296755 17.58691791001079 13.953778548584525 7 28.50985998548762 33.99096775003015 20.4564638999273 17.042708364554937 8 27.201229692472317 34.95421765696181 19.714187123781375 18.1303655267845 9 27.548190545241596 16.702058686149705 19.251120669822434 36.49863009878626 10-11 24.658146982677774 27.726404628743197 27.097399260477673 20.51804912810136 12-13 25.394774919894424 25.578346565135686 27.082832128698893 21.944046386271 14-15 22.50543715265057 27.205807252023483 24.64721689942688 25.64153869589907 16-17 22.14993262136694 30.40320517334051 25.738045259758053 21.7088169455345 18-19 22.22802459353285 28.86267983332398 27.464312220874664 21.44498335226851 20-21 22.96519096668441 28.156896703790117 27.367992813061154 21.509919516464315 22-23 22.55024875968803 28.467277728773492 27.114910589457608 21.867562922080864 24-25 22.162658148194502 28.68336126379804 27.359014486306638 21.794966101700826 26-27 21.563503015452756 28.911145774426423 27.156298004411088 22.369053205709733 28-29 21.97344259140743 29.199938371530436 27.033146914184446 21.793472122877684 30-31 21.71838881918854 28.843116345822345 26.97487196845601 22.463622866533107 32-33 21.3501829645167 29.002098244119324 27.00017325591083 22.647545535453144 34-35 22.38948375320885 28.967401838091238 26.804242306775222 21.838872101924682 36-37 22.620116944556905 28.442223521451332 26.597256241816424 22.340403292175335 38-39 21.956101728742166 28.83801623588886 26.69904045457981 22.506841580789168 40-41 22.294324497157163 28.836889026971075 26.995488799321233 21.873297676550536 42-43 21.9049685896958 28.761102638577025 26.685033758152155 22.64889501357502 44-45 21.848759694891353 28.90673117386567 27.21380039741042 22.030708733832558 46-47 22.427505988620574 29.220113682761273 26.266702990896185 22.085677337721968 48-49 21.810014062502177 29.438677595186697 26.155452669018313 22.595855673292814 50-51 22.114140895116833 29.39592241972427 26.42857267272236 22.06136401243653 52-53 22.35406167187874 28.981818650523117 26.509599631293895 22.154520046304246 54-55 21.718650181862767 29.213200568752207 26.76552486618927 22.302624383195756 56-57 21.951664615369612 29.64336685758705 26.2470597495119 22.15790877753144 58-59 21.76398626731912 28.895164301717703 26.50911796506638 22.831731465896805 60-61 21.28442616641176 29.28285735483181 26.863076953621484 22.569639525134946 62-63 21.495133055819448 29.261132344441904 27.032346776000033 22.211387823738622 64-65 21.314714110036196 29.463266109506677 26.61596719746665 22.60605258299048 66-67 21.34737236711502 29.622758452737934 26.423526878708476 22.60634230143857 68-69 21.97547804023625 28.937571058387128 26.781437902940038 22.305512998436587 70-71 21.63621375318193 28.979301867451955 26.551070793703662 22.833413585662452 72-73 21.617287585741888 29.190798677958956 26.561700185204224 22.630213551094936 74-75 21.545668508075543 29.68420795899773 26.34897572719972 22.421147805727006 76-77 21.740792215606625 29.147052439364728 26.75839929090888 22.353756054119764 78-79 21.954419345644002 29.15722064217402 26.612595798891498 22.27576421329048 80-81 22.026667484317137 29.279459458706096 26.450307524846583 22.24356553213018 82-83 21.862261421971567 29.34651301597611 26.590528900446103 22.200696661606223 84-85 21.74940140540812 29.286884994578504 26.659479815027996 22.30423378498538 86-87 22.07703933281446 29.26813888039198 26.36125652626217 22.29356526053138 88-89 21.818614477896283 29.221871955782813 26.36488936644048 22.594624199880425 90-91 21.852048865809227 29.19059428273237 26.468097498917487 22.489259352540916 92-93 22.056632463775184 28.71255811037008 26.795965324503634 22.434844101351104 94-95 21.744313109135565 28.387655015084807 27.161154380585828 22.706877495193798 96-97 22.13889774146191 28.0409459075861 26.9775574212716 22.842598929680385 98-99 21.6572376193051 28.41885512366507 26.735901604127637 23.188005652902195 100-101 21.647385521245468 28.28970182115954 27.086945501692757 22.97596715590223 102-103 21.436682733781392 27.965552365959812 27.32796200149415 23.269802898764645 104-105 21.141684009850977 28.47846021767118 27.011606297995478 23.368249474482365 106-107 21.442596446617447 28.59921501675592 26.55483985965831 23.403348676968324 108-109 21.50139585539927 28.55351220059093 26.489289621367696 23.455802322642107 110-111 21.48247414279162 28.922496459097395 26.70207975571864 22.89294964239234 112-113 21.407571424392323 29.239354077483398 26.82484115917237 22.528233338951907 114-115 21.120152889948766 29.63078526038373 26.527611010973644 22.721450838693862 116-117 21.219906441757313 29.498877705016447 26.597137538416753 22.68407831480949 118-119 20.933045398506284 29.76363123951205 26.314788384275246 22.98853497770642 120-121 20.90946490159516 30.0038700782341 26.47049910075545 22.616165919415288 122-123 21.00878347603348 29.935481983845424 26.34492624025161 22.710808299869488 124-125 21.01119346069543 29.99176736323689 26.40026216281537 22.596777013252318 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 8142.0 1 4787.5 2 1923.0 3 2465.0 4 2239.0 5 1772.0 6 1444.5 7 1258.5 8 1236.5 9 1216.5 10 1060.5 11 937.5 12 893.0 13 861.0 14 862.5 15 882.5 16 975.5 17 1083.0 18 1231.0 19 1436.0 20 1698.0 21 2188.5 22 2835.0 23 3684.5 24 4611.0 25 6134.0 26 8598.0 27 11261.0 28 13717.0 29 16212.0 30 19006.5 31 23007.5 32 29538.0 33 31921.5 34 31067.0 35 33351.0 36 36979.5 37 40495.5 38 42077.0 39 43348.0 40 44616.0 41 45177.5 42 45024.5 43 45578.0 44 46490.0 45 48244.5 46 48684.5 47 47977.0 48 48028.5 49 47787.0 50 47582.0 51 46730.0 52 45874.5 53 45803.5 54 47166.0 55 45160.5 56 41370.0 57 38286.0 58 35676.5 59 31304.5 60 24908.5 61 21256.0 62 18628.5 63 13396.0 64 8564.5 65 6617.5 66 5303.0 67 4358.5 68 3306.5 69 2328.0 70 1723.5 71 1140.5 72 815.0 73 747.0 74 625.5 75 457.5 76 321.5 77 186.0 78 108.5 79 72.5 80 46.0 81 26.5 82 18.5 83 14.5 84 10.5 85 7.5 86 6.0 87 5.0 88 7.5 89 7.0 90 4.0 91 2.5 92 2.5 93 4.5 94 4.0 95 2.5 96 1.5 97 1.0 98 1.5 99 2.0 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4617297597937265 2 0.4561817903158806 3 0.45188211397055 4 0.4485533322838424 5 0.457499433066869 6 0.5129791278453282 7 0.5084714026445784 8 0.5176255522830241 9 0.5242137660379662 10-11 0.540927024089977 12-13 0.5495263767806381 14-15 0.5287561660479525 16-17 0.5227574240500317 18-19 0.5332638912487023 20-21 0.5340267370519061 22-23 0.5446719034875229 24-25 0.537632917212506 26-27 0.5448106027244691 28-29 0.5247685629857507 30-31 0.5308366546021447 32-33 0.5322236469716062 34-35 0.5308713294113813 36-37 0.4805928560184026 38-39 0.4469929658681983 40-41 0.4459873964003387 42-43 0.4585396773439651 44-45 0.4630474025447149 46-47 0.4679018758378301 48-49 0.48177179953244487 50-51 0.4633594758278437 52-53 0.4632207765908976 54-55 0.4539626025247422 56-57 0.43634779943258145 58-59 0.4550028468018383 60-61 0.4499056498440674 62-63 0.466410859040659 64-65 0.46561333842821867 66-67 0.47348452012491254 68-69 0.4833321659480891 70-71 0.4793445628858872 72-73 0.4900590789399772 74-75 0.48035013235374685 76-77 0.4779922453256623 78-79 0.48974700565684837 80-81 0.48288139342801406 82-83 0.484233710988239 84-85 0.4813557018216065 86-87 0.4719935033277414 88-89 0.4765012285284913 90-91 0.4612096376551784 92-93 0.46197248345838227 94-95 0.4566325628359556 96-97 0.4449818269324791 98-99 0.40864262685258834 100-101 0.4057646176859558 102-103 0.3954315245334678 104-105 0.3998699001157445 106-107 0.39945380240490613 108-109 0.4113819367822748 110-111 0.43326174141052964 112-113 0.43378186354907766 114-115 0.42754039788650106 116-117 0.4210908833685052 118-119 0.4639836223941014 120-121 0.4575341078761055 122-123 0.42240852611949353 124-125 0.43135462690252013 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1441969.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.80042661561134 #Duplication Level Percentage of deduplicated Percentage of total 1 79.71889657985838 46.87505128221473 2 10.81404688482541 12.717411405379142 3 3.801244060456628 6.705443172747255 4 1.7784231044563066 4.1828814898036315 5 1.0096821211787796 2.968486973573382 6 0.6355945049778009 2.2423936828339803 7 0.42312691232965355 1.7416030070271011 8 0.3113015858673289 1.464373284409225 9 0.21184780709221615 1.1211067291143642 >10 1.1813238387427987 12.671561513440546 >50 0.08475940734649484 3.3868370330107216 >100 0.02900311237577057 2.8241935574199237 >500 5.00053661628362E-4 0.23757921856632572 >1k 0.0 0.0 >5k 2.50026830814181E-4 0.8610776504597585 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 5857 0.40618071539679423 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 3.4674809236536986E-5 0.0 0.0 0.0 0.0 14-15 2.080488554192219E-4 0.0 0.0 3.4674809236536986E-5 0.0 16-17 2.080488554192219E-4 0.0 0.0 3.120732831288329E-4 0.0 18-19 2.080488554192219E-4 0.0 0.0 5.201221385480548E-4 0.0 20-21 2.080488554192219E-4 0.0 0.0 6.588213754942028E-4 0.0 22-23 2.080488554192219E-4 0.0 0.0 0.0010749190863326465 0.0 24-25 2.080488554192219E-4 0.0 0.0 0.0016990656525903121 0.0 26-27 2.080488554192219E-4 0.0 0.0 0.002808659548159496 0.0 28-29 2.080488554192219E-4 0.0 0.0 0.006449514517995879 0.0 30-31 2.080488554192219E-4 0.0 0.0 0.018308299276891527 0.0 32-33 2.080488554192219E-4 0.0 0.0 0.04108964894529633 0.0 34-35 2.080488554192219E-4 0.0 0.0 0.07264372535054497 0.0 36-37 2.080488554192219E-4 0.0 0.0 0.12202065370337364 0.0 38-39 2.773984738922959E-4 0.0 0.0 0.2010098691442049 0.0 40-41 2.773984738922959E-4 0.0 0.0 0.28825168918333194 0.0 42-43 2.773984738922959E-4 0.0 0.0 0.3705696863108707 0.0 44-45 4.160977108384438E-4 0.0 0.0 0.47095325905064533 0.0 46-47 4.160977108384438E-4 0.0 0.0 0.5895064318303653 0.0 48-49 4.160977108384438E-4 0.0 0.0 0.7189128199011213 0.0 50-51 4.160977108384438E-4 0.0 0.0 0.8800813332325452 0.0 52-53 4.160977108384438E-4 0.0 0.0 1.0254381335521083 0.0 54-55 4.160977108384438E-4 0.0 0.0 1.181752173590417 0.0 56-57 4.160977108384438E-4 0.0 0.0 1.3652165892609343 0.0 58-59 4.160977108384438E-4 0.0 0.0 1.5751725591881658 0.0 60-61 4.160977108384438E-4 0.0 0.0 1.8165439062836994 0.0 62-63 4.160977108384438E-4 0.0 0.0 2.0518124869536027 0.0 64-65 4.160977108384438E-4 0.0 0.0 2.280319479822382 0.0 66-67 4.160977108384438E-4 0.0 0.0 2.5444721765863205 0.0 68-69 4.160977108384438E-4 0.0 0.0 2.853563426120811 0.0 70-71 4.160977108384438E-4 0.0 0.0 3.2073158299519617 0.0 72-73 4.507725200749808E-4 0.0 0.0 3.5669976261625598 0.0 74-75 4.854473293115178E-4 0.0 0.0 3.9064986833974933 0.0 76-77 4.854473293115178E-4 0.0 0.0 4.274051661304785 0.0 78-79 4.854473293115178E-4 0.0 0.0 4.702077506520598 0.0 80-81 5.547969477845918E-4 0.0 0.0 5.186970038884331 0.0 82-83 6.241465662576658E-4 0.0 0.0 5.695198717864253 0.0 84-85 6.241465662576658E-4 0.0 0.0 6.203115323561048 0.0 86-87 6.241465662576658E-4 0.0 0.0 6.7085353429928105 0.0 88-89 6.241465662576658E-4 0.0 0.0 7.2515428556369805 0.0 90-91 6.934961847307398E-4 0.0 0.0 7.876313568460903 0.0 92-93 6.934961847307398E-4 0.0 0.0 8.529933722569625 0.0 94-95 6.934961847307398E-4 0.0 0.0 9.177693833917374 0.0 96-97 6.934961847307398E-4 0.0 0.0 9.825939392594432 0.0 98-99 8.321954216768876E-4 0.0 0.0 10.493498820016242 0.0 100-101 9.015450401499616E-4 0.0 0.0 11.2488895392342 0.0 102-103 9.708946586230356E-4 0.0 0.0 12.05133397458614 0.0 104-105 9.708946586230356E-4 0.0 0.0 12.848958611454199 0.0 106-107 0.0010055694678595727 0.0 0.0 13.614162301686097 0.0 108-109 0.0010402442770961096 0.0 0.0 14.426627756907395 0.0 110-111 0.0010402442770961096 0.0 0.0 15.293393963393111 0.0 112-113 0.0010402442770961096 0.0 0.0 16.21720023107293 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2465 0.0 57.218536 1 GTATCAA 4355 0.0 53.15777 1 ATCAACG 5365 0.0 40.8281 3 CAACGCA 5445 0.0 40.341774 5 TCAACGC 5445 0.0 40.12172 4 TATCAAC 5610 0.0 40.100464 2 GTACATG 9855 0.0 38.889698 1 AACGCAG 5625 0.0 38.733353 6 TACATGG 9795 0.0 38.157127 2 ACATGGG 9980 0.0 35.188644 3 CATGGGA 7070 0.0 33.341656 4 ACGCAGA 6750 0.0 31.839062 7 TAATGCG 75 0.0039015785 31.748648 5 CGCAGAG 6735 0.0 31.468008 8 ATGGGAG 2870 0.0 30.905155 5 TCGACCG 75 5.276008E-6 27.781523 18-19 ATGGGAT 2395 0.0 27.340956 5 GTACCGT 175 5.6780846E-7 27.213127 6 GGTAAGG 820 0.0 26.131824 3 GAGTACA 6775 0.0 26.000858 1 >>END_MODULE